In vitro evaluation of Saanen buck semen frozen in different extenders supplemented with various antioxidants
Abstract
The aim of this present study was to investigate the effects of extenders containing different antioxidants upon the principle sperm characteristics of Saanen buck semen following freezing. In this study, four bucks were used. Semen was collected twice a week by an artificial vagina during the breeding season to obtain ten ejaculates per animal. The samples were then extended in three extenders (tris, T-, skimmed milk, M- and laiciphos 488, L- based) without (as controls) or with the two antioxidants (5 mM cysteine, C and 1000 µg/ml hyaluronic acid, H). By this way, nine experimental groups were assigned, as follows: T-C, T-H, T-O (control); M-C, M-H, M-O (control); L-C, L-H, L-O (control). For the T extender groups, the rates of motility and viability were significantly (p<0.01) higher for the T-C extender as compared to those of the T-H and T-O. However, there was no such difference (p>0.05) among the extenders based on the rates of abnormal spermatozoa, abnormal acrosome and hypoosmotic swelling (HOS) test. For the M groups, the motility was significantly (p<0.01) higher for the M-C and M-O extenders as compared to that of the MH. However, there was no such difference (p>0.05) among the extenders based on the rates of viability, abnormal spermatozoa, abnormal acrosome and HOS test. For the L groups, the rates of motility and viability were significantly (p<0.01) higher for the L-C extender as compared to that of the L-H but not that of the L-O. Additionally, there was no such difference (p>0.05) among the extenders mentioned above based on the rates of abnormal spermatozoa, abnormal acrosome and HOS test. As a result, the present in vitro findings of semen quality parameters studied suggest that; i) both skimmed milk and laiciphos extenders were more favourable than tris, and that ii) the cysteine, as an antioxidant, provided a superior protection than the hyaluronic acid did
References
- Aitken RJ, Clarkson JS (1989): Significance of reactive species and antioxidants in defining the efficacy of sperm preparative techniques. J Androl, 9, 367-376.
- Alvarez JG, Storey BT (1983): Role of superoxide dismutase in protecting rabbit spermatozoa from O2 toxicity due to lipid peroxidation. Biol Reprod, 28, 1129- 1136.
- Ateşşahin A, Bucak MN, Tuncer PB, Kızıl M (2008): Effect of anti-oxidant additives on microscopic and oxidative parameters of Angora goat semen following the freeze-thawing process. Small Rum Res, 77, 38-44.
- Aurich JE, Schonherr U, Hoppe H, Aurich C (1997): Effect of antioxidants on motility and membrane integrity of chilled stored stallion semen. Theriogenology, 48, 185- 192.
- Balogh GT, Illes J, Szekely Z, Forrai E, Gere A (2003): Effect of different metal ions on the oxidative damage and antioxidant capacity of hyaluronic acid. Arch Biochem Biophys, 410, 76-82.
- Bucak MN, Uysal O (2008): The role of antioxidants in freezing of Saanen goat semen. Indian Vet J, 85, 148-150.
- Daniel WW (1991): Analysis of variance. In: Biostatistics: Daniel, W.W. (Ed.). A Foundation for Analysis in the Health Sciences. John Wiley and Sons, Hoboken, pp: 274- 320.
- Donnelly ET, McClure N, Sheena E, Lewis EM (1999): Antioxidant supplementation in vitro does not improve human sperm motility. Fertil Steril, 72, 484-495.
- Evans G, Maxwell WMC (1987): Salamon’s Artificial Insemination of Sheep and Goats. Sidney: Butterworths, pp. 8-21, 107-141.
- Foote RH, Brockett CC, Kaproth MT (2002): Motility and fertility of bull sperm in whole milk extender containing antioxidants. Animal Reprod Sci, 71, 13-23.
- Funahashi H, Sano T (2005): Select antioxidants improve the function of extended boar semen stored at 10oC. Theriogenology, 63, 1605-1616.
- Huszar G, Willets M, Corrales M (1990): Hyaluronic acid (sperm select) improves retention of sperm motility and velocity in normospermic and oligospermic specimens. Fertil Steril, 54, 1127-1134.
- Griveau JF, Le Lannou D (1997): Reactive oxygen species and human spermatozoa: physiology and pathology. Int J Androl, 20, 61-69.
- Güney O, Tuncel E, Biçer O (1989): The potential of the Mediterranean goat populations with special reference to Mediterranean and Aegean parts of Turkey. Proceedings of the International Symposium on the Constraints and Possibilities of Ruminant Production in the Dry Subtropics. 5-7 November, 1988. EAAP publication, 38.
- Mara L, Dattena M, Pilichi S, Sana D, Branca A, Cappai P (2007): Effect of different diluents on goat semen fertility. Anim Reprod Sci, 102, 152-157.
- Mari G, Lacono E, Rizzato G, Merlo B, Belluzzi S (2005): Effect of hyaluronic acid supplementation on motility of stallion spermatozoa after cryopreservation. Anim Reprod Sci, 89, 284-285.
- Maxwell WMC, Stojanov T (1996): Liquid storage of ram semen in the absence or presence of some antioxidants. Reprod Fertil Dev, 8, 1013-1020.
- Meister A, Tate SS (1976): Glutathione and related gamma-glutamyl compounds: biosynthesis and utilization. Ann Rev Biochem, 45, 559-604.
- Neild DM, Gabella BM, Chaves MG, Miragaya MH, Colenbrander B, Aguero A (2003): Membrane changes during different stages of a freeze-thaw protocol for equine semen cryopreservation. Theriogenology, 59, 1693-1705.
- Pena, FJ, Johannisson AM, Rodrıguez-Martines H (2004): Effect of hyaluronan supplementation on boar sperm motility and membrane lipid architecture status after cryopreservation. Theriogenology, 61, 63-70.
- Purdy PH (2006): A review on goat sperm cryopreservation. Small Rum Res, 63, 215-225.
- Revell G, Mrode RA (1994): An osmotic resistance test for bovine semen. Anim Reprod Sci, 36, 77-86.
- Schafer S, Holzmann H (2000): The use of transmigration and spermac stain to evaluate epididymal cat spermatozoa. Anim Reprod Sci, 59, 201-211.
- Sinha MP, Sinha AK, Singh BK, Prasad RL (1996): The effect of glutathione on the motility, enzyme leakage and fertility of frozen goat semen. Anim Reprod Sci, 41, 237-243.
- Upreti GC, Jensen K, Oliver JE, Duganzich DM, Munday R, Smith JF (1997): Motility of ram spermatozoa drying storage in a chemically-defined diluent containing antioxidants. Animal Reprod Sci, 48, 269-278.
- Uysal O, Bucak MN (2007): Effects of oxidized glutathione, bovine serum albumin, cysteine and lycopene on the quality of frozen-thawed ram semen. Acta Vet Brno, 76, 383-390.
Farklı antioksidanlar eklenmiş sulandırıcılarla dondurulmuş Saanen tekesi spermasının in vitrodeğerlendirilmesi
Abstract
Sunulan çalışmada, Saanen teke spermasının farklı antioksidanlar içeren sulandırıcılarla dondurulmasının başlıca spermatolojik özellikler üzerine etkileri araştırıldı. Araştırmada, dört adet teke kullanıldı. Her bir tekeden aşım mevsiminde suni vajen yardımıyla haftada iki kez toplam on ejekulat alındı. Split-sperma örnekleri, üç farklı sulandırıcı (tris, T-, yağsız süt tozu, Y- ve laiciphos 488, L) ve iki farklı antioksidan (5 mM sistein, S ve 1000 µg/ml hyaluronik asit, H) kullanılarak, antioksidan kullanılmadan (kontrol grupları) sulandırıldı. Böylece, örnekler toplam dokuz ayrı çalışma grubuna ayrıldı: T-S, T-H, T-K (kontrol); Y-S, Y-H, YK; L-S, L-H, L-K. T sulandırıcı grupları için, T-S grubundaki motilite ve canlılık oranları, T-H ve T-K gruplarındaki oranlardan istatistiksel olarak daha yüksek (p<0.01) olmasına karşın, anılan gruplar arasında anormal spermatozoa, anormal akrozom ve HOS testi oranları yönlerinden önemli bir fark belirlenmedi (p>0.05). Y grupları için, Y-S ve Y-K sulandırıcı gruplarındaki motilite oranları, Y-H grubundaki orandan daha yüksek (p<0.01) olmasına karşın, gruplar arasında canlılık, anormal spermatozoa, anormal akrozom ve HOS testi oranları yönlerinden önemli bir fark belirlenmedi (p>0.05). L grupları için, L-S grubundaki motilite ve canlılık oranları, L-H grubundaki oranlardan daha yüksek (p<0.01) iken, L-K grubundaki değerlerden ise önemli bir farklılık belirlenmedi (p>0.05). Ayrıca, anılan gruplar arasında anormal spermatozoa, anormal akrozom ve HOS testi oranları yönünden önemli bir fark belirlenmedi (p>0.05). Sonuç olarak, sunulan çalışmada elde edilen in vitro sperma kalitesi bulgularına göre; i) yağsız süt tozu ve laiciphos sulandırıcısının tris sulandırıcından daha üstün olduğu, ayrıca ii) sisteinin bir antioksidan olarak hyaluronik asitten daha iyi koruma sağladığı kanısına varıldı
Keywords
Antioksidanlar in vitro değerlendirme Saanen tekesi spermanın dondurulması spermanın sulandırılması
References
- Aitken RJ, Clarkson JS (1989): Significance of reactive species and antioxidants in defining the efficacy of sperm preparative techniques. J Androl, 9, 367-376.
- Alvarez JG, Storey BT (1983): Role of superoxide dismutase in protecting rabbit spermatozoa from O2 toxicity due to lipid peroxidation. Biol Reprod, 28, 1129- 1136.
- Ateşşahin A, Bucak MN, Tuncer PB, Kızıl M (2008): Effect of anti-oxidant additives on microscopic and oxidative parameters of Angora goat semen following the freeze-thawing process. Small Rum Res, 77, 38-44.
- Aurich JE, Schonherr U, Hoppe H, Aurich C (1997): Effect of antioxidants on motility and membrane integrity of chilled stored stallion semen. Theriogenology, 48, 185- 192.
- Balogh GT, Illes J, Szekely Z, Forrai E, Gere A (2003): Effect of different metal ions on the oxidative damage and antioxidant capacity of hyaluronic acid. Arch Biochem Biophys, 410, 76-82.
- Bucak MN, Uysal O (2008): The role of antioxidants in freezing of Saanen goat semen. Indian Vet J, 85, 148-150.
- Daniel WW (1991): Analysis of variance. In: Biostatistics: Daniel, W.W. (Ed.). A Foundation for Analysis in the Health Sciences. John Wiley and Sons, Hoboken, pp: 274- 320.
- Donnelly ET, McClure N, Sheena E, Lewis EM (1999): Antioxidant supplementation in vitro does not improve human sperm motility. Fertil Steril, 72, 484-495.
- Evans G, Maxwell WMC (1987): Salamon’s Artificial Insemination of Sheep and Goats. Sidney: Butterworths, pp. 8-21, 107-141.
- Foote RH, Brockett CC, Kaproth MT (2002): Motility and fertility of bull sperm in whole milk extender containing antioxidants. Animal Reprod Sci, 71, 13-23.
- Funahashi H, Sano T (2005): Select antioxidants improve the function of extended boar semen stored at 10oC. Theriogenology, 63, 1605-1616.
- Huszar G, Willets M, Corrales M (1990): Hyaluronic acid (sperm select) improves retention of sperm motility and velocity in normospermic and oligospermic specimens. Fertil Steril, 54, 1127-1134.
- Griveau JF, Le Lannou D (1997): Reactive oxygen species and human spermatozoa: physiology and pathology. Int J Androl, 20, 61-69.
- Güney O, Tuncel E, Biçer O (1989): The potential of the Mediterranean goat populations with special reference to Mediterranean and Aegean parts of Turkey. Proceedings of the International Symposium on the Constraints and Possibilities of Ruminant Production in the Dry Subtropics. 5-7 November, 1988. EAAP publication, 38.
- Mara L, Dattena M, Pilichi S, Sana D, Branca A, Cappai P (2007): Effect of different diluents on goat semen fertility. Anim Reprod Sci, 102, 152-157.
- Mari G, Lacono E, Rizzato G, Merlo B, Belluzzi S (2005): Effect of hyaluronic acid supplementation on motility of stallion spermatozoa after cryopreservation. Anim Reprod Sci, 89, 284-285.
- Maxwell WMC, Stojanov T (1996): Liquid storage of ram semen in the absence or presence of some antioxidants. Reprod Fertil Dev, 8, 1013-1020.
- Meister A, Tate SS (1976): Glutathione and related gamma-glutamyl compounds: biosynthesis and utilization. Ann Rev Biochem, 45, 559-604.
- Neild DM, Gabella BM, Chaves MG, Miragaya MH, Colenbrander B, Aguero A (2003): Membrane changes during different stages of a freeze-thaw protocol for equine semen cryopreservation. Theriogenology, 59, 1693-1705.
- Pena, FJ, Johannisson AM, Rodrıguez-Martines H (2004): Effect of hyaluronan supplementation on boar sperm motility and membrane lipid architecture status after cryopreservation. Theriogenology, 61, 63-70.
- Purdy PH (2006): A review on goat sperm cryopreservation. Small Rum Res, 63, 215-225.
- Revell G, Mrode RA (1994): An osmotic resistance test for bovine semen. Anim Reprod Sci, 36, 77-86.
- Schafer S, Holzmann H (2000): The use of transmigration and spermac stain to evaluate epididymal cat spermatozoa. Anim Reprod Sci, 59, 201-211.
- Sinha MP, Sinha AK, Singh BK, Prasad RL (1996): The effect of glutathione on the motility, enzyme leakage and fertility of frozen goat semen. Anim Reprod Sci, 41, 237-243.
- Upreti GC, Jensen K, Oliver JE, Duganzich DM, Munday R, Smith JF (1997): Motility of ram spermatozoa drying storage in a chemically-defined diluent containing antioxidants. Animal Reprod Sci, 48, 269-278.
- Uysal O, Bucak MN (2007): Effects of oxidized glutathione, bovine serum albumin, cysteine and lycopene on the quality of frozen-thawed ram semen. Acta Vet Brno, 76, 383-390.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery |
| Other ID | JA93FH84ME |
| Journal Section | Research Article |
| Authors | |
| Publication Date | September 1, 2010 |
| Published in Issue | Year 2010Volume: 57 Issue: 3 |