Evaluation of the effect of platelet rich plasma (PRP) on enhancement of bone healing in diaphyseal bone defects by radiography and computed tomography
Abstract
The effect of platelet-rich plasma (PRP) with autogenous cancellous bone graft on enhancement of bone healing in diaphyseal bone defects was evaluated. A 4-mm defect was created in the middiaphysis of the tibias of 20 rabbits. Rabbits were divided into two groups of ten animals each: only autogenous cancellous graft, PRP and autogenous cancellous graft. In animals of group 1, only autogenous cancellous grafts, and to those in group 2, PRP and autogenous cancellous grafts, were applied to the defect. Radiographical and computed tomography (CT) views were taken and evaluated on postoperative days 0, 15, 30, 60, and 90. According to the bone formation, union, and remodeling scores, group 1 had better scores than group 2 on days 30, 60, and 90. The density was significantly increased on day 60 than on days 0, 15, and 30 in group 1. In conclusion, it was evaluated that PRP could not enhance the bone regeneration in diaphyseal defects when used with autogenous cancellous bone graft
References
- Aghaloo TL, Moy PK, Freymiller EG (2004): Evaluation of platelet-rich plasma in combination with anorganic bovine bone in the rabbit cranium: A pilot study. Int J Oral Maxillofac Implants, 19, 59–65.
- Akca K, Cehreli M, Demiralp B, Guzel E, Dagdeviren A (2007): Platelet-rich plasma and bone healing: A histologic study in titanium bone chambers. Int J Periodontics Restorative Dent, 27, 387–392.
- Borzini P, Mazzucco L (2007): Platelet- Rich plasma (PRP) and platelet derivatives for topical therapy. What is true from the biological wiev point? ISBT Sci Ser, 2, 272- 281.
- Boudrieau RJ (2002): Fractures of the tibia and fibula. 2144. In: Slatter D. (Ed), Textbook of Small Animal Surgery. Saunders Co., New York.
- Choi BH, Im CJ, Huh JY, Suh JJ, Lee SH (2004): Effect of platelet-rich plasma on bone regeneration in autogenous bone graft. Int J Oral Maxillofac Surg, 33, 56– 59.
- Dallari D, Fini M, Stagni C, Torricelli P, Nicoli Aldini N, Giavaresi G, Cenni E, Baldini N, Cenacchi A, Bassi A, Giardino R, Fornasari PM, Giunti (2006): In vivo study on the healing of bone defects treated with bone marrow stromal cells, platelet-rich plasma, and freeze- dried bone allografts, alone and in combination. J Orthop Res, 24, 877-888.
- Dugrillon A, Klüter H (2002): Topical application of platelets for improved wound healing. Blood Ther Med, 3, 21–26.
- Hatakeyama M, Beletti ME, Zanetta-Barbosa D, Dechichi P (2008): Radiographic and histomorphometric analysis of bone healing using autogenous graft associated with platelet-rich plasma obtained by 2 different methods. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 105, 13-18.
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- Kasten P, Vogel J, Geiger F, Niemeyer P, Luginbühl R, Szalay K (2008): The effect of platelet-rich plasma on healing in critical-size long-bone defects. Biomaterials, 29, 3983–3992.
- Katz MH, Alvarez AF, Kirsner RS, Eaglstein WH, Falanga V (1991): Human wound fluid from acute wounds stimulates fibroblast and endothelial cell growth. J Am Acad Dermatol, 25, 1054-1062.
- Lane JM, Sandhu HS (1987): Current approaches to experimental bone grafting. Orthop Clin North Am, 18, 213-225.
- Marcellin-Little DJ (2002): External skeletal fixation. 1820-1826. In: Slatter D. (Ed), Textbook of Small Animal Surgery. Saunders Co., New York.
- Marx RE, Carlson ER, Eichstaedt RM, Schimmele SR, Strauss JE, Georgeff KR (1998): Platelet-rich plasma: Growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 85, 638–646.
- Marx RE (2001): The biology of platelet-rich plasma. J Oral Maxillofac Surg, 59, 1119–1121.
- Mehta S, Watson JT (2008): Platelet rich concentrate: Basic science and current clinical applications. J Orthop Trauma, 22, 432-438.
- Messora MR, Nagata MJ, Dornelles RC, Bomfim SR, Furlaneto FA, de Melo LG, Deliberador TM, Bosco AF, Garcia VG, Fucini SE (2008): Bone healing in critical- size defects treated with platelet-rich plasma activated by two different methods. A histologic and histometric study in rat calvaria. J Periodontal Res, 43, 723-729.
- Mooren RE, Merkx MA, Bronkhorst EM, Jansen JA, Stoelinga PJ (2007): The effect of platelet-rich plasma on early and late bone healing: An experimental study in goats. Int J Oral Maxillofac Surg, 36, 626-631.
- Rai B, Oest ME, Dupont KM, Ho KH, Teoh SH, Guldberg RE (2007): Combination of platelet-rich plasma with polycaprolactone-tricalcium phosphate scaffolds for segmental bone defect repair. J Biomed Mater Res A, 81, 888-899.
- Sarkar MR, Augat P, Shefelbine SJ, Schorlemmer S, Huber-Lang M, Claes L, Kinzl L, Ignatius A (2006): Bone formation in a long bone defect model using a platelet-rich plasma-loaded collagen scaffold. Biomaterials, 27, 1817-1823.
- Thibault L, Beausejour A, de Grandmont MJ, Lemieux R, Leblanc JF (2006) : Characterization of blood components prepared from whole-blood donations after a 24-hour hold with the platelet-rich plasma method. Transfusion, 46, 1292-1299.
- Wiltfang J, Kloss FR, Kessler P, Nkenke E, Schultze- Mosgau S, Zimmermann R, Schlegel KA (2004): Effects of platelet-rich plasma on bone healing in combination with autogenous bone and bone substitutes in critical-size defects. Clin Oral Implants Res, 15, 187–193.
Diyafizeal kemik defektlerinde trombositten zengin plazmanın (PRP) kemik iyileşmesini artırmaüzerindeki etkisinin radyografi ve bilgisayarlı tomografi ile değerlendirilmesi
Abstract
Bu çalışmada; trombositten zengin plazmanın (PRP), otojen kansellöz greftler ile birlikte diyafizer defektlerde kemik rejenerasyonuna olan etkisi değerlendirildi. Yirmi tavşanda tibianın orta 1/3 diyafizinde 4 mm. genişliğinde defekt oluşturuldu. Tavşanlar, her birinde 10 tavşan içeren iki gruba ayrıldı, birinci gruptaki tavşanlara sadece otojenöz kansellöz greft, ikinci gruptakilere PRP ile birlikte otojenöz kansellöz greft yerleştirildi. Operasyon sonrası 0., 15., 30., 60. ve 90. günlerde radyografi ve bilgisayarlı tomografi (BT) çekimleri yapıldı ve değerlendirildi. Kemik oluşumu, kaynama ve yeniden şekillenme derecelendirmesine göre 30., 60. ve 90. günlerde birinci grupta, ikinci gruba göre daha iyi sonuçlar elde edildi. Birinci grupta dansite, 60. günde 0., 15. ve 30. güne göre daha belirgin bir artış gösterdi. Sonuç olarak; PRP, otojenöz kansellöz greftler ile birlikte diyafizer kemik defektlerine uygulandığında, kemik rejenerasyonunu artırıcı hiçbir katkısı olmadığı anlaşıldı
Keywords
Bilgisayarlı tomografi (BT) kemik rejenerasyonu radyografi trombositten zengin plazma (PRP)
References
- Aghaloo TL, Moy PK, Freymiller EG (2004): Evaluation of platelet-rich plasma in combination with anorganic bovine bone in the rabbit cranium: A pilot study. Int J Oral Maxillofac Implants, 19, 59–65.
- Akca K, Cehreli M, Demiralp B, Guzel E, Dagdeviren A (2007): Platelet-rich plasma and bone healing: A histologic study in titanium bone chambers. Int J Periodontics Restorative Dent, 27, 387–392.
- Borzini P, Mazzucco L (2007): Platelet- Rich plasma (PRP) and platelet derivatives for topical therapy. What is true from the biological wiev point? ISBT Sci Ser, 2, 272- 281.
- Boudrieau RJ (2002): Fractures of the tibia and fibula. 2144. In: Slatter D. (Ed), Textbook of Small Animal Surgery. Saunders Co., New York.
- Choi BH, Im CJ, Huh JY, Suh JJ, Lee SH (2004): Effect of platelet-rich plasma on bone regeneration in autogenous bone graft. Int J Oral Maxillofac Surg, 33, 56– 59.
- Dallari D, Fini M, Stagni C, Torricelli P, Nicoli Aldini N, Giavaresi G, Cenni E, Baldini N, Cenacchi A, Bassi A, Giardino R, Fornasari PM, Giunti (2006): In vivo study on the healing of bone defects treated with bone marrow stromal cells, platelet-rich plasma, and freeze- dried bone allografts, alone and in combination. J Orthop Res, 24, 877-888.
- Dugrillon A, Klüter H (2002): Topical application of platelets for improved wound healing. Blood Ther Med, 3, 21–26.
- Hatakeyama M, Beletti ME, Zanetta-Barbosa D, Dechichi P (2008): Radiographic and histomorphometric analysis of bone healing using autogenous graft associated with platelet-rich plasma obtained by 2 different methods. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 105, 13-18.
- John PWM (1971): Statistical Design and Analysis of Experiments. Siam, Philadelphia.
- Kasten P, Vogel J, Geiger F, Niemeyer P, Luginbühl R, Szalay K (2008): The effect of platelet-rich plasma on healing in critical-size long-bone defects. Biomaterials, 29, 3983–3992.
- Katz MH, Alvarez AF, Kirsner RS, Eaglstein WH, Falanga V (1991): Human wound fluid from acute wounds stimulates fibroblast and endothelial cell growth. J Am Acad Dermatol, 25, 1054-1062.
- Lane JM, Sandhu HS (1987): Current approaches to experimental bone grafting. Orthop Clin North Am, 18, 213-225.
- Marcellin-Little DJ (2002): External skeletal fixation. 1820-1826. In: Slatter D. (Ed), Textbook of Small Animal Surgery. Saunders Co., New York.
- Marx RE, Carlson ER, Eichstaedt RM, Schimmele SR, Strauss JE, Georgeff KR (1998): Platelet-rich plasma: Growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 85, 638–646.
- Marx RE (2001): The biology of platelet-rich plasma. J Oral Maxillofac Surg, 59, 1119–1121.
- Mehta S, Watson JT (2008): Platelet rich concentrate: Basic science and current clinical applications. J Orthop Trauma, 22, 432-438.
- Messora MR, Nagata MJ, Dornelles RC, Bomfim SR, Furlaneto FA, de Melo LG, Deliberador TM, Bosco AF, Garcia VG, Fucini SE (2008): Bone healing in critical- size defects treated with platelet-rich plasma activated by two different methods. A histologic and histometric study in rat calvaria. J Periodontal Res, 43, 723-729.
- Mooren RE, Merkx MA, Bronkhorst EM, Jansen JA, Stoelinga PJ (2007): The effect of platelet-rich plasma on early and late bone healing: An experimental study in goats. Int J Oral Maxillofac Surg, 36, 626-631.
- Rai B, Oest ME, Dupont KM, Ho KH, Teoh SH, Guldberg RE (2007): Combination of platelet-rich plasma with polycaprolactone-tricalcium phosphate scaffolds for segmental bone defect repair. J Biomed Mater Res A, 81, 888-899.
- Sarkar MR, Augat P, Shefelbine SJ, Schorlemmer S, Huber-Lang M, Claes L, Kinzl L, Ignatius A (2006): Bone formation in a long bone defect model using a platelet-rich plasma-loaded collagen scaffold. Biomaterials, 27, 1817-1823.
- Thibault L, Beausejour A, de Grandmont MJ, Lemieux R, Leblanc JF (2006) : Characterization of blood components prepared from whole-blood donations after a 24-hour hold with the platelet-rich plasma method. Transfusion, 46, 1292-1299.
- Wiltfang J, Kloss FR, Kessler P, Nkenke E, Schultze- Mosgau S, Zimmermann R, Schlegel KA (2004): Effects of platelet-rich plasma on bone healing in combination with autogenous bone and bone substitutes in critical-size defects. Clin Oral Implants Res, 15, 187–193.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery |
| Other ID | JA87RT67ER |
| Journal Section | Research Article |
| Authors | |
| Publication Date | June 1, 2010 |
| Published in Issue | Year 2010Volume: 57 Issue: 2 |