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Fertilizing ability of short-term preserved spermatozoa Abant trout (Salmo trutta abanticus T, 1954)

Year 2010, Volume: 57 Issue: 1, 33 - 38, 01.03.2010

Taner Hatipoğlu Ergun Akçay

https://doi.org/10.1501/Vetfak_0000002306
Cited By: 5

Abstract

The objective of this experiment was to evaluate spermatological parameters and fertilizing ability of short term stored semen in different extenders from endemic Abant trout (Salmo trutta abanticus T,1954). Semen was collected from 30 adult males by the hand stripping method without anesthesia. Having determined the main spermatological properties (volume, motility, movement duration, concentration and pH), the pooled samples were diluted at a 1:2 ratio with two extenders (0.3 M glucose or Ringer solution). The diluted semen was stored for 48 hours at 4°C. Following the cooled storage, motility of the spermatozoa was evaluated after 24 and 48 hours regarding the post-cooled period. Dry fertilization technique was used for the fertilization trials. Eggs were pooled from 30 females by the hand stripping method. Fertilization took place in dry plastic dishes and 600 eggs were used in each fertilization trial. The sperm-egg ratio was approximately 0.25x106 sperm/egg. After swelling, eggs were rinsed with hatchery water (7oC) and batches were placed into vertical incubation trays. The experimental success was assessed from sperm motility and the percent of eyed-egg 25 days after fertilization. Average fresh semen motility was 81.4 %. According to the results of the experiment, the highest motility (67 % after 24 h, 53 % after 48 h) and movement duration (60 s after 24 h, 42 s after 48 h) were determined by using glucose extender after 24 and 48 hours storage. Fertility rate of fresh semen was 84.4 %. The highest eyed-egg rate (80.4 %) was obtained from semen stored with glucose based extender after 24 h storage. After 48 hour storage fertilization yield for the semen diluted with 0.3 M Glucose decreased to 61.9 % and to 43.8 % for the one diluted with Ringer solution. Our results indicate that glucose based extender is a better preservative than Ringer solution for the short term preservation of Abant trout semen

Keywords

Abant trout semen short-term preservation fertility

References

  • Akçay E, Tekin N, Seçer S (1995): Balık spermasının prezervasyonu. Ege University J Fish Aquat Sci, 12, 367- 373.
  • Akçay E, Bozkurt Y, Kayam S (2002): Cryopreservation of mirror carp (Cyprinus carpio L. 1758) semen: with emphasis on post-thaw motility. pp. 5. In: 1st Int Cong Aquacult-Fish Technol Environ Management., June 8-10, Athens.
  • Akçay E., Bozkurt Y., Tekin N., Seçer S (2004): Cryopreservation of mirror carp semen. Turk J Vet Anim Sci, 28, 837-345
  • Baynes SM, Scott AP (1985): Seasonal variations in parameters of milt production and in plasma concentration of sex steroids of male Rainbovv trout (Salmo gairdneri). Gen Comp Endocrinol, 57, 150-160.
  • Benau D, Terner C (1980): Initiation, prolongation and reactivation of the motility of salmonid spermatozoa. Gamete Res, 3,247- 257.
  • Bencic DC, Cloud JG, Ingermann RL (2000): Short- term storage of salmonid sperm in air versus oxygen. N Am J Aquaculture, 62, 19-25.
  • Billard R (1981): Short-term preservation of sperm under oxygen atmosphere in rainbow trout (Salmo Gairdneri). Aquaculture, 23, 287-293.
  • Billard R (1992): Reproduction in Rainbow trout: sex differentiation, dynamics of gametogenesis, biology and preservation of gametes. Aquaculture, 100, 263-298.
  • Billard R, Cosson MP (1992): Some problems related to the assesment of sperm motility in freshwater fishes. J Exp Zool, 261, 122-131.
  • Bozkurt Y, Secer S (2004): Determination of some spermatological properties of mirror carp (Cyprinus carpio L. 1758) semen and evaluation of short-term preservation using different extenders. In: 3th Nat. Cong. Reprod. Art. Insem., September 30-October 2. Antalya.
  • Bozkurt Y, Secer S, Bukan N, Akcay E, Tekin N (2006): Relationship between body condition, physiological and biochemical parameters in brown trout (Salmo trutta fario) sperm. Pakistan J Biol Sci, 9, 940-944.
  • Büyükhatipoglu Ş, Holtz W (1978): Preservation of trout sperm in liquid or frozen state. Aquaculture, 14, 49-56.
  • Büyükhatipoglu Ş, Holtz W (1984): Sperm output in Rainbow trout (Salmo gairdneri) effect of age, timing and frequency of stripping and presence of females. Aquaculture, 37, 63-71.
  • Ciereszko A, Dabrowski K (1994): Relationship between biochemical constituents of fish semen and fertility: The effect of short-term storage. Fish Physiol and Biochem, 12, 357–357.
  • Çevik M (2000): Alabalık (Gökkuşağı Alabalığı) Spermasının Dondurulması ve Değerlendirilmesi. Ankara Üniversitesi, Sağlık Bilimleri Enstitüsü, Doktora Tezi, Ankara
  • Erdahl AW, Erdahl DA, Graham EF (1984): Some factors affecting the preservation of Salmonid spermatozoa. Aquaculture, 43, 341-350.
  • Geldiay R, Kocataş A (1988): Deniz Biyolojisine Giriş. Ege Üniversitesi Fen Fakültesi Yayınları.
  • Gjerde B (1984): Variation in semen production of farmed Atlantic salmon and Rainbow trout. Aquaculture, 40, 109- 114.
  • Glogowski J, Kwasnik M, Piros B, Dabrowski K, Goryczko K, Dobosz S, Kuzminski H, Ciereszko A (2000): Characterization of rainbow trout milt collected with a catheter: semen parameters and cryopreservation success. Aquacult Res, 31, 289–296.
  • Hatipoğlu T, Akçay E (2008): Short-term storage of Abant trout (Salmo trutta abanticus Tontonese, 1954) semen and fertility trials. 16th International Congress on Animal Reproduction, 13-17 July Budapest, Rep Dom Anim, 43, 141.
  • Jenkins JA, Tiersch TR (1997): A preliminary bacteriological study of refrigerated channel catfish sperm. J World Aquaculture Soc, 28, 282-288.
  • Jensen JOT, Aiderdice DF (1984): Effect of temperature on short-term storage of eggs and sperm of chum salmon (Oncorhynchus keta). Aquaculture, 37, 251-265.
  • Lahnsteiner F, Berger B, Weismann T, Patzner RA (1996): Motility of spermatozoa of Alburnus alburnus (Cyprinidae) and its relationship to seminal plasma composition and sperm metabolism. Fish Physiol Biochem, 15, 167-179.
  • Levanduski MJ, Cloud JG (1988): Rainbow trout (Salmo gairdneri) semen: effect of non-motile sperm on fertility. Aquaculture, 75, 171-179
  • Linhart O, Rodino M, Cosson J (2000): Cryopreservation of sperm in common carp Cyprinus carpio: sperm motility and hatching success of embryos. Cryobiology, 41, 241- 250.
  • Magyary I, Urbanyi B, Horvath L (1996): Cryopreservation of common carp (Cyprinus carpio L.) sperm: II. Optimal conditions for fertilization. J App Ichthyol, 12, 117-119.
  • Mansour N, Lahnsteiner F, Berger B (2004): Characterization of the testicular semen of the African catfish, Clarias gariepinus (Burchell, 1822) and its short- term storage. Aquacult Res, 35, 232-244.
  • Mc Niven MA, Gallant RK, Richardson GF (1993): Fresh storage of Rainbow trout (Oncorhynchus mykiss) semen using a non-aqueous medium. Aquaculture, 109, 71- 82.
  • Munkittrick KR, Moccia RD (1987): Seasonal changes in the quality of Rainbow trout (Salmo gairdneri) semen: effect of a delay in stripping on spermatocrit, motility, volume and seminal plasma contituents. Aquaculture, 64, 147-156.
  • Piironen J (1985): Variation in the properties of milt from the Finnish landlocked salmon (Salmo salar Salmo salar m. sebago Girard) during a spavvning season. Aquaculture, 48, 337-350.
  • Rana K (1995): Preservation of gametes. 53-75. In: NR Bromage, RJ Roberts (Eds), Broodstock Management and Egg and Larval Quality. Oxford Blackwell Science, England.
  • Saad A, Billard R, Theron MC, Hollebecq MG (1988): Short-term preservation of carp (Cyprinus carpio) semen. Aquaculture , 71, 133-150.
  • Scott AP, Baynes SM (1980): A review of the biology, handling and storage of salmonid spermatozoa. J Fish Biol, 17, 707-739.
  • Schmidt-Baulain R, Holtz W (1989): Deep freezing of rainbow trout (Salmogairdneri) sperm at varying intervals after collection. Theriogenology, 32, 439-443.
  • Stoss J (1983): Fish gamete preservation and spermatozoan physiology. 305–350. In: W Hoar, D Randall, E Donaldson (Eds), Fish Physiology. Academic Pres, New York.
  • Stoss J, Holtz W (1983): Successfull storage of chilled rainbow trout (Salmo gairdneri). Aquaculture, 31, 269-274.
  • Stoss J, Refstie T (1983): Short-term storage and cryopreservation of milt from Atlantic salmon and sea trout. Aquaculture, 30, 229-236.
  • Tekin N, Seçer S, Akçay E, Bozkurt Y, Kayam S (2003): Gökkuşağı alabalıklarında (Oncorhynchus mykiss W., 1792) yaşın spermatolojik özellikler üzerine etkisi. Turk J Vet Anim Sci, 27, 37-44.
  • Tekin N, Seçer S, Akçay E, Bozkurt Y (2003): Cryopreservation of rainbow trout (Oncorhynchus mykiss) semen. Isr J Aquacult-Bamid, 55, 208-212.
  • Truscott B, Idler DR, Hoyle RJ, Freeman HC (1968): Sub-zero preservation of Atlantic salmon sperm. J Fish Res Bd Can, 25, 363–72.
  • Uysal İ, Alpbaz A (2003): Abant alabalığı (Salmo trutta abanticus T., 1954) ile gökkuşağı alabalığı (Oncorhynchus mykiss w., 1792) yumurtalarının döllenme, gözlenme, larva çıkışı ve yaşama oranlarının karşılaştırılması. Ege University J Fish Aquat Sci, 20, 95-101. Geliş tarihi: 26.12.2008 / Kabul tarihi: 15.05.2009 Address for correspondence Assoc. Prof. Ergun Akçay Department of Animal Reproduction and Artificial Insemination Faculty of Veterinary Medicine University of Ankara 06110 Dışkapı, Ankara, Turkey E-mail: akcay@veterinary.ankara.edu.tr

Kısa süreli saklanmış Abant alabalığı spermatozoonlarının fertilizasyon yeteneği

Year 2010, Volume: 57 Issue: 1, 33 - 38, 01.03.2010

Taner Hatipoğlu Ergun Akçay

https://doi.org/10.1501/Vetfak_0000002306
Cited By: 5

Abstract

Bu araştırma ile, ülkemize ait endemik bir balık türü olan Abant alabalığının spermatolojik parametrelerinin değerlendirilmesi ve farklı sulandırıcılarda kısa süreli saklanan spermatozoonların fertilizasyon yeteneğinin ortaya konulması amaçlanmıştır. Bu araştırmada, 30 ergin erkek balıktan anestezi yapılmaksızın masaj yoluyla sperma alınmıştır. Birincil spermatolojik parametreler (miktar, motilite, haraket süresi, yoğunluk ve pH) belirlendikten sonra birleştirilen örnekler iki farklı sulandırıcı ile (0,3 M glukoz veya Ringer solusyonu) 1:2 (sperma:sulandırıcı) oranında sulandırılmıştır. Sulandırılan sperma 4°C‘de 48 saat saklanmıştır. Saklama işlemi esnasında 24. ve 48. saatlerde motilite değerlendirmesi yapılmıştır. Fertilizasyon denemeleri için kuru fertilizasyon tekniği kullanılmıştır. Yumurtalar 30 ergin dişiden masaj yoluyla alınmıştır. Fertilizasyon kuru plastic kaplarda gerçekleştirilmiş ve her bir fertilizasyon denemesi için 600 yumurta kullanılmıştır. Tohumlama dozu olarak herbir yumurta için 0,25x106 spermatozoa kullanılmıştır. Yumurtaların şişmesinden sonra üzerlerine kuluçka suyu (7oC) eklenerek dikey inkubasyon tepsilerine yerleştirilmiştir. Deneysel başarının tesbiti için spermatozoon motilitesi ve fertilizasyon sonrası 25. günde gözlü yumurta sayısı dikkate alınmıştır. Araştırmadan elde edilen sonuçlara göre taze sperma motilitesi ortalama % 81,4 olarak bulundu. Saklama sonrası en yüksek motilite (24. saat: % 67; 48. saat: % 53) ve hareket süreleri (24. saat: 60 saniye; 48. saat: 42 saniye) glukoz sulandırıcısı ile elde edildi. Taze spermadan elde edilen fertilizasyon oranı % 84,4 olarak bulundu. En yüksek gözlü yumurta oranı (% 80,4) 24 saat glukoz sulandırıcısında saklanan spermadan elde edildi. Saklamanın 48. saatinde bu oran glukoz sulandırıcısı için % 61,9’a, ringer sulandırıcısı için % 43,8’e düştü. Sonuçlara göre, Abant alabalığı spermasının kısa süreli saklanmasında glukoz tabanlı sulandırıcı Ringer sulandırıcısına oranla daha iyi koruyucu özellliklere sahip olduğu tesbit edilmiştir

Keywords

Abant alabalığı fertilite kısa süreli saklama sperma

References

  • Akçay E, Tekin N, Seçer S (1995): Balık spermasının prezervasyonu. Ege University J Fish Aquat Sci, 12, 367- 373.
  • Akçay E, Bozkurt Y, Kayam S (2002): Cryopreservation of mirror carp (Cyprinus carpio L. 1758) semen: with emphasis on post-thaw motility. pp. 5. In: 1st Int Cong Aquacult-Fish Technol Environ Management., June 8-10, Athens.
  • Akçay E., Bozkurt Y., Tekin N., Seçer S (2004): Cryopreservation of mirror carp semen. Turk J Vet Anim Sci, 28, 837-345
  • Baynes SM, Scott AP (1985): Seasonal variations in parameters of milt production and in plasma concentration of sex steroids of male Rainbovv trout (Salmo gairdneri). Gen Comp Endocrinol, 57, 150-160.
  • Benau D, Terner C (1980): Initiation, prolongation and reactivation of the motility of salmonid spermatozoa. Gamete Res, 3,247- 257.
  • Bencic DC, Cloud JG, Ingermann RL (2000): Short- term storage of salmonid sperm in air versus oxygen. N Am J Aquaculture, 62, 19-25.
  • Billard R (1981): Short-term preservation of sperm under oxygen atmosphere in rainbow trout (Salmo Gairdneri). Aquaculture, 23, 287-293.
  • Billard R (1992): Reproduction in Rainbow trout: sex differentiation, dynamics of gametogenesis, biology and preservation of gametes. Aquaculture, 100, 263-298.
  • Billard R, Cosson MP (1992): Some problems related to the assesment of sperm motility in freshwater fishes. J Exp Zool, 261, 122-131.
  • Bozkurt Y, Secer S (2004): Determination of some spermatological properties of mirror carp (Cyprinus carpio L. 1758) semen and evaluation of short-term preservation using different extenders. In: 3th Nat. Cong. Reprod. Art. Insem., September 30-October 2. Antalya.
  • Bozkurt Y, Secer S, Bukan N, Akcay E, Tekin N (2006): Relationship between body condition, physiological and biochemical parameters in brown trout (Salmo trutta fario) sperm. Pakistan J Biol Sci, 9, 940-944.
  • Büyükhatipoglu Ş, Holtz W (1978): Preservation of trout sperm in liquid or frozen state. Aquaculture, 14, 49-56.
  • Büyükhatipoglu Ş, Holtz W (1984): Sperm output in Rainbow trout (Salmo gairdneri) effect of age, timing and frequency of stripping and presence of females. Aquaculture, 37, 63-71.
  • Ciereszko A, Dabrowski K (1994): Relationship between biochemical constituents of fish semen and fertility: The effect of short-term storage. Fish Physiol and Biochem, 12, 357–357.
  • Çevik M (2000): Alabalık (Gökkuşağı Alabalığı) Spermasının Dondurulması ve Değerlendirilmesi. Ankara Üniversitesi, Sağlık Bilimleri Enstitüsü, Doktora Tezi, Ankara
  • Erdahl AW, Erdahl DA, Graham EF (1984): Some factors affecting the preservation of Salmonid spermatozoa. Aquaculture, 43, 341-350.
  • Geldiay R, Kocataş A (1988): Deniz Biyolojisine Giriş. Ege Üniversitesi Fen Fakültesi Yayınları.
  • Gjerde B (1984): Variation in semen production of farmed Atlantic salmon and Rainbow trout. Aquaculture, 40, 109- 114.
  • Glogowski J, Kwasnik M, Piros B, Dabrowski K, Goryczko K, Dobosz S, Kuzminski H, Ciereszko A (2000): Characterization of rainbow trout milt collected with a catheter: semen parameters and cryopreservation success. Aquacult Res, 31, 289–296.
  • Hatipoğlu T, Akçay E (2008): Short-term storage of Abant trout (Salmo trutta abanticus Tontonese, 1954) semen and fertility trials. 16th International Congress on Animal Reproduction, 13-17 July Budapest, Rep Dom Anim, 43, 141.
  • Jenkins JA, Tiersch TR (1997): A preliminary bacteriological study of refrigerated channel catfish sperm. J World Aquaculture Soc, 28, 282-288.
  • Jensen JOT, Aiderdice DF (1984): Effect of temperature on short-term storage of eggs and sperm of chum salmon (Oncorhynchus keta). Aquaculture, 37, 251-265.
  • Lahnsteiner F, Berger B, Weismann T, Patzner RA (1996): Motility of spermatozoa of Alburnus alburnus (Cyprinidae) and its relationship to seminal plasma composition and sperm metabolism. Fish Physiol Biochem, 15, 167-179.
  • Levanduski MJ, Cloud JG (1988): Rainbow trout (Salmo gairdneri) semen: effect of non-motile sperm on fertility. Aquaculture, 75, 171-179
  • Linhart O, Rodino M, Cosson J (2000): Cryopreservation of sperm in common carp Cyprinus carpio: sperm motility and hatching success of embryos. Cryobiology, 41, 241- 250.
  • Magyary I, Urbanyi B, Horvath L (1996): Cryopreservation of common carp (Cyprinus carpio L.) sperm: II. Optimal conditions for fertilization. J App Ichthyol, 12, 117-119.
  • Mansour N, Lahnsteiner F, Berger B (2004): Characterization of the testicular semen of the African catfish, Clarias gariepinus (Burchell, 1822) and its short- term storage. Aquacult Res, 35, 232-244.
  • Mc Niven MA, Gallant RK, Richardson GF (1993): Fresh storage of Rainbow trout (Oncorhynchus mykiss) semen using a non-aqueous medium. Aquaculture, 109, 71- 82.
  • Munkittrick KR, Moccia RD (1987): Seasonal changes in the quality of Rainbow trout (Salmo gairdneri) semen: effect of a delay in stripping on spermatocrit, motility, volume and seminal plasma contituents. Aquaculture, 64, 147-156.
  • Piironen J (1985): Variation in the properties of milt from the Finnish landlocked salmon (Salmo salar Salmo salar m. sebago Girard) during a spavvning season. Aquaculture, 48, 337-350.
  • Rana K (1995): Preservation of gametes. 53-75. In: NR Bromage, RJ Roberts (Eds), Broodstock Management and Egg and Larval Quality. Oxford Blackwell Science, England.
  • Saad A, Billard R, Theron MC, Hollebecq MG (1988): Short-term preservation of carp (Cyprinus carpio) semen. Aquaculture , 71, 133-150.
  • Scott AP, Baynes SM (1980): A review of the biology, handling and storage of salmonid spermatozoa. J Fish Biol, 17, 707-739.
  • Schmidt-Baulain R, Holtz W (1989): Deep freezing of rainbow trout (Salmogairdneri) sperm at varying intervals after collection. Theriogenology, 32, 439-443.
  • Stoss J (1983): Fish gamete preservation and spermatozoan physiology. 305–350. In: W Hoar, D Randall, E Donaldson (Eds), Fish Physiology. Academic Pres, New York.
  • Stoss J, Holtz W (1983): Successfull storage of chilled rainbow trout (Salmo gairdneri). Aquaculture, 31, 269-274.
  • Stoss J, Refstie T (1983): Short-term storage and cryopreservation of milt from Atlantic salmon and sea trout. Aquaculture, 30, 229-236.
  • Tekin N, Seçer S, Akçay E, Bozkurt Y, Kayam S (2003): Gökkuşağı alabalıklarında (Oncorhynchus mykiss W., 1792) yaşın spermatolojik özellikler üzerine etkisi. Turk J Vet Anim Sci, 27, 37-44.
  • Tekin N, Seçer S, Akçay E, Bozkurt Y (2003): Cryopreservation of rainbow trout (Oncorhynchus mykiss) semen. Isr J Aquacult-Bamid, 55, 208-212.
  • Truscott B, Idler DR, Hoyle RJ, Freeman HC (1968): Sub-zero preservation of Atlantic salmon sperm. J Fish Res Bd Can, 25, 363–72.
  • Uysal İ, Alpbaz A (2003): Abant alabalığı (Salmo trutta abanticus T., 1954) ile gökkuşağı alabalığı (Oncorhynchus mykiss w., 1792) yumurtalarının döllenme, gözlenme, larva çıkışı ve yaşama oranlarının karşılaştırılması. Ege University J Fish Aquat Sci, 20, 95-101. Geliş tarihi: 26.12.2008 / Kabul tarihi: 15.05.2009 Address for correspondence Assoc. Prof. Ergun Akçay Department of Animal Reproduction and Artificial Insemination Faculty of Veterinary Medicine University of Ankara 06110 Dışkapı, Ankara, Turkey E-mail: akcay@veterinary.ankara.edu.tr
There are 41 citations in total.

Details

Primary Language English
Subjects Veterinary Surgery
Other ID JA85BH99CD
Journal Section Research Article
Authors

Taner Hatipoğlu

Ergun Akçay

Publication Date March 1, 2010
Published in Issue Year 2010Volume: 57 Issue: 1

Cite

APA Hatipoğlu, T., & Akçay, E. (2010). Fertilizing ability of short-term preserved spermatozoa Abant trout (Salmo trutta abanticus T, 1954). Ankara Üniversitesi Veteriner Fakültesi Dergisi, 57(1), 33-38. https://doi.org/10.1501/Vetfak_0000002306
AMA Hatipoğlu T, Akçay E. Fertilizing ability of short-term preserved spermatozoa Abant trout (Salmo trutta abanticus T, 1954). Ankara Univ Vet Fak Derg. March 2010;57(1):33-38. doi:10.1501/Vetfak_0000002306
Chicago Hatipoğlu, Taner, and Ergun Akçay. “Fertilizing Ability of Short-Term Preserved Spermatozoa Abant Trout (Salmo Trutta Abanticus T, 1954)”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 57, no. 1 (March 2010): 33-38. https://doi.org/10.1501/Vetfak_0000002306.
EndNote Hatipoğlu T, Akçay E (March 1, 2010) Fertilizing ability of short-term preserved spermatozoa Abant trout (Salmo trutta abanticus T, 1954). Ankara Üniversitesi Veteriner Fakültesi Dergisi 57 1 33–38.
IEEE T. Hatipoğlu and E. Akçay, “Fertilizing ability of short-term preserved spermatozoa Abant trout (Salmo trutta abanticus T, 1954)”, Ankara Univ Vet Fak Derg, vol. 57, no. 1, pp. 33–38, 2010, doi: 10.1501/Vetfak_0000002306.
ISNAD Hatipoğlu, Taner - Akçay, Ergun. “Fertilizing Ability of Short-Term Preserved Spermatozoa Abant Trout (Salmo Trutta Abanticus T, 1954)”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 57/1 (March 2010), 33-38. https://doi.org/10.1501/Vetfak_0000002306.
JAMA Hatipoğlu T, Akçay E. Fertilizing ability of short-term preserved spermatozoa Abant trout (Salmo trutta abanticus T, 1954). Ankara Univ Vet Fak Derg. 2010;57:33–38.
MLA Hatipoğlu, Taner and Ergun Akçay. “Fertilizing Ability of Short-Term Preserved Spermatozoa Abant Trout (Salmo Trutta Abanticus T, 1954)”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 57, no. 1, 2010, pp. 33-38, doi:10.1501/Vetfak_0000002306.
Vancouver Hatipoğlu T, Akçay E. Fertilizing ability of short-term preserved spermatozoa Abant trout (Salmo trutta abanticus T, 1954). Ankara Univ Vet Fak Derg. 2010;57(1):33-8.

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