Abstract
Nasal swab samples from clinically healthy Anatolian water buffalo calves, breeding in Afyonkarahisar province of Turkey, were collected to determine the bacterial microflora of their nasal mucosa. A total of 160 samples were examined and 165 bacterial isolates were identified by using standard microbiological and biochemical methods. Ninety-seven isolates were detected to Gram positive bacteria (58.8%) and 68 isolates to Gram negative bacteria (41.2%). Ten bacterial genera including Staphylococcus, Micrococcus, Corynebacterium, Arcanobacterium, Bacillus, Escherichia, Neisseria, Moraxella, Pasteurella and Mannheimia were isolated. Staphylococcus epidermidis (48.8%), Staphylococcus aureus (33.8%), Mannheimia haemolytica (25.0%) and Pasteurella multocida (17.5%) were determined as the most frequently isolated species from the animals sampled.Also, the isolation rates of S. epidermidis, S. aureus, M. haemolytica and P. multocida among the 165 bacterial isolates recovered from samples were 23.6%, 16.3%, 12.1% and 8.4% respectively
References
- Aly R, Shinefield HR, Litz C, Maibach HI (1980): Role of teichoic acid in the binding of Staphylococcus aureus to nasal epithelial cells. J Infect Dis, 141, 463-465.
- Baker JC (1998): Respiratory Diseases. 1049-1125. In:, SE Aillo, A Mays (Eds.), The Merck Veterinary Manual. NJ: Merck and Rhone-Poulence Company.
- Barbour EK, Nabbut NH, Hamadeh SK, Al-Nakhli HM (1997): Bacterial identity and characteristics in healthy and unhealthy respiratory tracts of sheep and calves. Vet Res Comm, 21, 421-430.
- Ghandrasekaran S, Yeap PC, Chuink BH (1981): Biochemical and serological studies of Pasteurella multocida isolated from cattle and buffaloes in Malaysia. Br Vet J, 137, 361-367.
- Hajikolaei MRH, Ghorbanpour M, Sayfi- Abadshapouri MR, Rasooli A, Jahferian H (2006): Occurence of Pasteurella multocida in the nasopharynx of
- Ankara Üniv Vet Fak Derg, 57, 2010 67
- healthy buffaloes and their immunity status. Bull Vet Inst
- Pulawy, 50, 435-438.
- Hiramune T, De Alwis MCL (1982): Haemorrhagic septicaemia carrier status of cattle and buffalo in Sri Lanka. Trop Anim Health Prod, 14, 91-92.
- Holth JG, Krieg NR, Sneath PHA, Staley JT, Williams ST (2000): Bergey’s Manuel of Determinative Bacteriology. Lippincott Williams&Wilkins, Philadelphia.
- Magwood SE, Barnum DA, Thomson RG (1969): Nasal bacterial flora of calves in healthy and in pneumonia- prone herds. Can J Comp Med, 33, 237-243.
- Marshall MM, Songer JG, Chilelli CJ, deVos JC (1983): Isolations of aerobic bacteria from wild desert bighorn sheep (Ovis canadensis nelsoni and O. c. mexicana) in Arizona. J Wildl Dis, 19, 98-100.
- McSween RN, Whaley K (1992): Respiratory system. 524-584. In: Muir’s Textbook of Pathology. Edward Arnold, London.
- Megra T, Sisay T, Asseged B (2006): The aerobic bacterial flora of the respiratory passageways of healthy goats in Dire Dawa Abattoir, Eastern Ethiopia. Revue Méd Vét, 157, 84-87.
- Queen C, Ward ACS, Hunter DL (1994): Bacteria isolated from nasal and tonsillar samples of clinally healthy Rocky Mountain bighorn and domestic sheep. J Wildl Dis, 30, 1-7.
- Quinn PJ, Carter ME, Markey B, Carter GR (1999): Clinical Veterinary Microbiology. Harcourt Publishers Limited.
- Quinn PJ, Markey BK, Carter ME, Donnelly WJ, Leonard FC (2002): Veterinary Microbiology and Microbial Disease. Blackwell Publishing Professional, Iowa. Geliş tarihi: 12.05.2008 / Kabul tarihi: 07.11.2008 Address for correspondence Esra Şeker, DVM, PhD Afyon Kocatepe University Faculty of Veterinary Medicine Department of Microbiology 03200, Afyonkarahisar, Turkey e-mail: eseker@aku.edu.tr
Abstract
Türkiye’nin Afyonkarahisar ilinde yetiştirilen klinik olarak sağlıklı Anadolu manda yavrularından nazal mukozanın bakteriyel mikroflorasını belirlemek amacıyla nazal sıvap örnekleri toplandı. Toplam 160 örnek incelendi ve 165 bakteriyel izolat standart mikrobiyolojik ve biyokimyasal yöntemler kullanılarak identifiye edildi. Doksanyedi izolat Gram pozitif (%58.8), 68 izolat Gram negatif (%41.2) olarak belirlendi. Staphylococcus, Micrococcus, Corynebacterium, Arcanobacterium, Bacillus, Escherichia, Neisseria, Moraxella, Pasteurella ve Mannheimia cinslerini içeren 10 cins izole edildi. Staphylococcus epidermidis (%48.8), Staphylococcus aureus (%33.8), Mannheimia haemolytica (%25.0) ve Pasteurella multocida (%17.5) örneklenen hayvanlardan en sık izole edilen türler olarak belirlendi. Ayrıca, S. epidermidis, S. aureus, M. haemolytica ve P. multocida türlerinin örneklerden elde edilen 165 bakteriyel izolat arasındaki izolasyon oranları sırasıyla %23.6, %16.3, %12.1 ve %8.4 şeklindeydi
Keywords
References
- Aly R, Shinefield HR, Litz C, Maibach HI (1980): Role of teichoic acid in the binding of Staphylococcus aureus to nasal epithelial cells. J Infect Dis, 141, 463-465.
- Baker JC (1998): Respiratory Diseases. 1049-1125. In:, SE Aillo, A Mays (Eds.), The Merck Veterinary Manual. NJ: Merck and Rhone-Poulence Company.
- Barbour EK, Nabbut NH, Hamadeh SK, Al-Nakhli HM (1997): Bacterial identity and characteristics in healthy and unhealthy respiratory tracts of sheep and calves. Vet Res Comm, 21, 421-430.
- Ghandrasekaran S, Yeap PC, Chuink BH (1981): Biochemical and serological studies of Pasteurella multocida isolated from cattle and buffaloes in Malaysia. Br Vet J, 137, 361-367.
- Hajikolaei MRH, Ghorbanpour M, Sayfi- Abadshapouri MR, Rasooli A, Jahferian H (2006): Occurence of Pasteurella multocida in the nasopharynx of
- Ankara Üniv Vet Fak Derg, 57, 2010 67
- healthy buffaloes and their immunity status. Bull Vet Inst
- Pulawy, 50, 435-438.
- Hiramune T, De Alwis MCL (1982): Haemorrhagic septicaemia carrier status of cattle and buffalo in Sri Lanka. Trop Anim Health Prod, 14, 91-92.
- Holth JG, Krieg NR, Sneath PHA, Staley JT, Williams ST (2000): Bergey’s Manuel of Determinative Bacteriology. Lippincott Williams&Wilkins, Philadelphia.
- Magwood SE, Barnum DA, Thomson RG (1969): Nasal bacterial flora of calves in healthy and in pneumonia- prone herds. Can J Comp Med, 33, 237-243.
- Marshall MM, Songer JG, Chilelli CJ, deVos JC (1983): Isolations of aerobic bacteria from wild desert bighorn sheep (Ovis canadensis nelsoni and O. c. mexicana) in Arizona. J Wildl Dis, 19, 98-100.
- McSween RN, Whaley K (1992): Respiratory system. 524-584. In: Muir’s Textbook of Pathology. Edward Arnold, London.
- Megra T, Sisay T, Asseged B (2006): The aerobic bacterial flora of the respiratory passageways of healthy goats in Dire Dawa Abattoir, Eastern Ethiopia. Revue Méd Vét, 157, 84-87.
- Queen C, Ward ACS, Hunter DL (1994): Bacteria isolated from nasal and tonsillar samples of clinally healthy Rocky Mountain bighorn and domestic sheep. J Wildl Dis, 30, 1-7.
- Quinn PJ, Carter ME, Markey B, Carter GR (1999): Clinical Veterinary Microbiology. Harcourt Publishers Limited.
- Quinn PJ, Markey BK, Carter ME, Donnelly WJ, Leonard FC (2002): Veterinary Microbiology and Microbial Disease. Blackwell Publishing Professional, Iowa. Geliş tarihi: 12.05.2008 / Kabul tarihi: 07.11.2008 Address for correspondence Esra Şeker, DVM, PhD Afyon Kocatepe University Faculty of Veterinary Medicine Department of Microbiology 03200, Afyonkarahisar, Turkey e-mail: eseker@aku.edu.tr
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery |
| Other ID | JA83EE48EH |
| Journal Section | Research Article |
| Authors | |
| Publication Date | March 1, 2010 |
| Published in Issue | Year 2010Volume: 57 Issue: 1 |