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Ankara tavşanı ince bağırsağında Paneth hücrelerinin morfolojisi, histokimyası ve ince yapısı üzerinde çalışmalar

Year 2009, Volume: 56 Issue: 1, 25 - 30, 01.03.2009

Emel Ergün Levent Ergün Asuman Özen Aytül Kürüm

https://doi.org/10.1501/Vetfak_0000002181
Cited By: 1

Abstract

Bu araştırma, Ankara tavşanı ince bağırsağındaki Paneth hücrelerinin morfolojisini, histokimyasal özelliklerini, yerleşim bölgelerini ve sayısal dağılımını ortaya koymak amacıyla yapıldı. Çalışmada materyal olarak özel yetiştiricilerden sağlanan 10 adet sağlıklı, erişkin Ankara tavşanının duodenum, jejunum ve ileumlarından alınan doku örnekleri kullanıldı. Lieberhkühn kriptlerinde gözlenen Paneth hücreleri, bazalinde çekirdeği ve apikalindeki asidofilik granülleriyle tanındı. Granüller, Mallory’nin üçlü boyama tekniği, Phloxine-tartrazine, Alcian blue-performik asit ve Mallory’nin fosfotungstik asit-hematoksilen uygulamasına pozitif reaksiyon, Periyodik asit-Schiff (PAS), Alcian blue (pH 2.5), PAS-Alcian blue pH 2.5 boyamalarına ise negatif reaksiyon verdi. Paneth hücrelerinin ince bağırsağın tüm uzunluğu boyunca homojen bir dağılım göstermediği, hücre yoğunluğunun duodenumdan ileuma doğru gidildikçe arttığı gözlendi. İnce bağırsağın üç bölgesi arasındaki farkın istatistiksel olarak önemli olduğu (p<0.01) saptandı. Elektron mikroskobik incelemelerde bazı Paneth hücrelerinin apikal sitoplazmasında elektron yoğun ve homojen granüller, bazılarında ise elektron yoğunlukları farklı olan homojen granüller görüldü

Keywords

Ankara tavşanı ince bağırsak Paneth hücresi

References

  • Ayabe T, Satchell DP, Wilson CL, Parks WC, Selsted ME, Ouellette AJ (2000): Secretion of microbicidal alpha-defensins by intestinal Paneth cells in response to bacteria. Nature Immunol, 1, 113-118.
  • Ayabe T, Ashida T, Kohgo Y, Kono T (2004): The role of Paneth cells and their antimicrobial peptides in innate host defense. Trends Microbiol, 12, 394-398.
  • Behnke O, Moe H (1964): An electron microscope study of mature and differentiating Paneth cells in the rat, especially of their endoplasmic reticulum and lysosomes. J Cell Biol, 22, 633-652.
  • Bjerknes M, Cheng H (1981): The stem-cell zone of the small intestinal epithelium. I. Evidence from Paneth cells in the adult mouse. Am J Anat, 160, 51-63.
  • Bloom W, Fawcett DW (1994): A Textbook of Histology, 12. Ed. Chapman and Hall, New York-London.
  • Cheng H, Merzel J, Leblond CP (1969): Renewal of Paneth cells in the small intestine of the mouse. Am J Anat, 126, 507-526.
  • Creamer B (1967): Paneth cell function. Lancet, 1, 314- 316.
  • Culling CFA, Allison RT and Barr WT (1985): Cellular Pathology Technique. Fourth edition, Butterworth, Wellington.
  • Dellman HD, Eurell JA (1998): Textbook of Veterinary Histology. 5. Ed. Williams and Wilkins, Baltimore.
  • Deschner EE (1967): Observations on the Paneth cell in human ileum. Exp Cell Res, 47, 624-628.
  • Dinsdale D (1984): Ultrastructural localization of zinc and calcium within the granules of rat Paneth cells. J Histochem Cytochem, 32, 139-145.
  • Dinsdale D, Biles B (1986) : Postnatal changes in the distribution and elemental composition of Paneth cells in normal and corticosteroid-treated rats. Cell Tissue Res, 246, 183-187.
  • Ergün E, Ergün L, Asti RN, Kürüm A (2003): Light and electron microscopic morphology of Paneth cells in the sheep small intestine. Revue Med Vet, 154, 351-355.
  • Garabedian EM, Roberts LJJ, McNevin MS, Gordon JI (1997): Examining the role of Paneth cells in the small intestine by lineage ablation in transgenic mice. J Biol Chem, 272, 23729-23740
  • Glerean A, Castro NM (1965): A histochemical study of the Paneth cells of the Tamandua tetradactyla (Edentata, Mammalia). Acta Anat, 61, 146-153.
  • Irmak MK, Özcan O, Karaöz E, Köksal V, Sarsılmaz M (1994): Variations in the number of Paneth cells in the ileum of three strains of rabbit. Tr J Med Sci, 20, 69-72.
  • Karnovsky MJ (1965): A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microsco . J Cell Biol, 27, 137A- 138A.
  • Krause WJ (1971): Paneth cells of the Echidna (Tachyglossus aculeatus). Acta Anat, 80, 435-448.
  • Lencer WI (1998): Paneth cells: on the front line or in the backfield? Gastroenterology, 114, 1343-1345.
  • Lewin K (1969): Histochemical observations on Paneth cells. J Anat, 105, 171-176.
  • Lopez-Lewellyn J, Erlandsen SL (1979): Morphometric analysis of a polarized cell: The intestinal Paneth cell. J Histochem Cytochem, 27, 1554-1556.
  • Mathan M, Hughes J, Whitehead R (1987): The morphogenesis of the human Paneth cell: An İmmunocytochemical ultrastructural study. Histochemistry, 87, 91-96.
  • Merzel J (1967): Some histophysiological aspects of Paneth’s cells of mice as shown by histochemical and radioautographical studies. Acta Anat, 66, 603- 630.
  • Ouellette AJ (1999): Mucosal immunity and inflammation IV. Paneth cell antimicrobial peptides and the biology of the mucosal barrier. Am J Physiol, 277, 257- 261.
  • Ouellette AJ, Satchell DP, Hsiech MM, Hagen S.J, Selsted ME (2000): Characterization luminal Paneth cell α-defensins in mouse small intestine. J Biol Chem, 275, 33969-33973.
  • Porter EM, Bevins CL, Ghosh D, Ganz T (2002): The multifaceted Paneth cell. Cell Mol Life Sci, 59, 156-170.
  • Riecken EO, Pearse AGE (1966): Histochemical study on the Paneth cell in the rat. Gut, 7, 86-93.
  • Rodning CB, Erlandsen SL, Wilson ID, Carpenter AM (1982): Light microscopic morphometric analysis of rat ileal mucosa: II. Component quantitation of Paneth cells. Anat Rec, 204, 33-38.
  • Sandow MJ, Whitehead R (1979) : The Paneth cell. Gut, 20, 420-431.
  • Satoh Y, Ishikawa K, Ono K, Vollrath L (1986): Quantitative light microscopic observations on Paneth cells of germ-free and ex-germ-free Wistar rats. Digestion, 34, 115-121.
  • Satoh Y, Yamano M, Matsuda M, Ono K (1990): Ultrastructure of Paneth cells in the intestine of various mammals. J Electron Microsc Tech, 16, 69-80.
  • Satoh Y, Ono K, Moutairou K (1994): Paneth cells of African giant rats (Cricetomys gambianus). Acta Anat, 151, 49-53.
  • Sawada M, Nishikawa M, Adachi T, Midorikawa O, Hiai H (1993) : A paneth cell specific zinc-binding protein in the rat. Lab Invest, 68, 338-344.
  • Singh I (1971): The distribution of Paneth cells in the human small intestine. Anat Anz Bd, 128, 60-65.
  • Subbuswamy SG (1973) Paneth cells and goblet cells. J Pathol, 111, 181-189.
  • Sümbüloğlu K, Sümbüloğlu V (2000): Biyoistatistik. Hatipoğlu Yayınları, Ankara.
  • Takehana K, Masty J, Yamaguchi M, Kobayashi A, Yamada O, Kuroda M, Park YS, Iwasa K, Abe M (1998): Fine structural and histochemical study of equine Paneth cells. Anat Histol Embryol, 27, 125-129.
  • Taylor JJ, Flaa RC, Forks G (1964) : Histochemical analysis of Paneth cell granules in rats. Arch Path, 77, 278-285.
  • Trier JS (1966) : The Paneth cells: an enigma. Gastroenterology, 51, 560-562.
  • Veneable JH, Coggeshall R (1965) : A simplified lead citrate stain for use in electron microscopy. J Cell Biol, 25, 407-408.
  • Wehkamp J, Stange EF (2006): Paneth cells and the innate immune response. Curr Opin Gastroenterol, 22, 644- 650. Geliş tarihi: 10.03.2008 / Kabul tarihi: 01.05.2008

Studies on the morphology, histochemistry and ultrastructure of Paneth cells in the small intestine ofthe Angora rabbit

Year 2009, Volume: 56 Issue: 1, 25 - 30, 01.03.2009

Emel Ergün Levent Ergün Asuman Özen Aytül Kürüm

https://doi.org/10.1501/Vetfak_0000002181
Cited By: 1

Abstract

The present study was undertaken to determine the morphology, histochemical properties, localization and quantitative distribution of Paneth cells in the small intestine of the Angora rabbit. Tissue samples taken from the duodenum, jejunum and ileum of 10 healthy adult Angora rabbits, obtained from private breeders, constituted the material of the study. The Paneth cells, which were determined to be located within the crypts of Lieberkühn, were identified on their basally located nucleus and apically located acidophilic granules. These granules gave positive reactions with Mallory’s triple staining technique as well as with the application of Phloxine-tartrazine, Alcian blue-performic acid and Mallory’s phosphotungstic acid-hematoxylin method. Staining with periodic acid-Schiff (PAS), Alcian blue (pH 2.5), and PAS-Alcian blue pH 2.5 gave negative reactions. Paneth cells were determined not to display a uniform distribution throughout the small intestine and cell numbers were ascertained to show a gradual increase from the duodenum towards the ileum. The difference between the three regions of the small intestine was determined to be statistically significant (p<0.01). Electron microscopic examination revealed the presence of electron-dense and homogenous granules in the apical cytoplasm of some Paneth cells, whereas homogenous granules of different electron density existed in some other cells

Keywords

Angora rabbit Paneth cell small intestine

References

  • Ayabe T, Satchell DP, Wilson CL, Parks WC, Selsted ME, Ouellette AJ (2000): Secretion of microbicidal alpha-defensins by intestinal Paneth cells in response to bacteria. Nature Immunol, 1, 113-118.
  • Ayabe T, Ashida T, Kohgo Y, Kono T (2004): The role of Paneth cells and their antimicrobial peptides in innate host defense. Trends Microbiol, 12, 394-398.
  • Behnke O, Moe H (1964): An electron microscope study of mature and differentiating Paneth cells in the rat, especially of their endoplasmic reticulum and lysosomes. J Cell Biol, 22, 633-652.
  • Bjerknes M, Cheng H (1981): The stem-cell zone of the small intestinal epithelium. I. Evidence from Paneth cells in the adult mouse. Am J Anat, 160, 51-63.
  • Bloom W, Fawcett DW (1994): A Textbook of Histology, 12. Ed. Chapman and Hall, New York-London.
  • Cheng H, Merzel J, Leblond CP (1969): Renewal of Paneth cells in the small intestine of the mouse. Am J Anat, 126, 507-526.
  • Creamer B (1967): Paneth cell function. Lancet, 1, 314- 316.
  • Culling CFA, Allison RT and Barr WT (1985): Cellular Pathology Technique. Fourth edition, Butterworth, Wellington.
  • Dellman HD, Eurell JA (1998): Textbook of Veterinary Histology. 5. Ed. Williams and Wilkins, Baltimore.
  • Deschner EE (1967): Observations on the Paneth cell in human ileum. Exp Cell Res, 47, 624-628.
  • Dinsdale D (1984): Ultrastructural localization of zinc and calcium within the granules of rat Paneth cells. J Histochem Cytochem, 32, 139-145.
  • Dinsdale D, Biles B (1986) : Postnatal changes in the distribution and elemental composition of Paneth cells in normal and corticosteroid-treated rats. Cell Tissue Res, 246, 183-187.
  • Ergün E, Ergün L, Asti RN, Kürüm A (2003): Light and electron microscopic morphology of Paneth cells in the sheep small intestine. Revue Med Vet, 154, 351-355.
  • Garabedian EM, Roberts LJJ, McNevin MS, Gordon JI (1997): Examining the role of Paneth cells in the small intestine by lineage ablation in transgenic mice. J Biol Chem, 272, 23729-23740
  • Glerean A, Castro NM (1965): A histochemical study of the Paneth cells of the Tamandua tetradactyla (Edentata, Mammalia). Acta Anat, 61, 146-153.
  • Irmak MK, Özcan O, Karaöz E, Köksal V, Sarsılmaz M (1994): Variations in the number of Paneth cells in the ileum of three strains of rabbit. Tr J Med Sci, 20, 69-72.
  • Karnovsky MJ (1965): A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microsco . J Cell Biol, 27, 137A- 138A.
  • Krause WJ (1971): Paneth cells of the Echidna (Tachyglossus aculeatus). Acta Anat, 80, 435-448.
  • Lencer WI (1998): Paneth cells: on the front line or in the backfield? Gastroenterology, 114, 1343-1345.
  • Lewin K (1969): Histochemical observations on Paneth cells. J Anat, 105, 171-176.
  • Lopez-Lewellyn J, Erlandsen SL (1979): Morphometric analysis of a polarized cell: The intestinal Paneth cell. J Histochem Cytochem, 27, 1554-1556.
  • Mathan M, Hughes J, Whitehead R (1987): The morphogenesis of the human Paneth cell: An İmmunocytochemical ultrastructural study. Histochemistry, 87, 91-96.
  • Merzel J (1967): Some histophysiological aspects of Paneth’s cells of mice as shown by histochemical and radioautographical studies. Acta Anat, 66, 603- 630.
  • Ouellette AJ (1999): Mucosal immunity and inflammation IV. Paneth cell antimicrobial peptides and the biology of the mucosal barrier. Am J Physiol, 277, 257- 261.
  • Ouellette AJ, Satchell DP, Hsiech MM, Hagen S.J, Selsted ME (2000): Characterization luminal Paneth cell α-defensins in mouse small intestine. J Biol Chem, 275, 33969-33973.
  • Porter EM, Bevins CL, Ghosh D, Ganz T (2002): The multifaceted Paneth cell. Cell Mol Life Sci, 59, 156-170.
  • Riecken EO, Pearse AGE (1966): Histochemical study on the Paneth cell in the rat. Gut, 7, 86-93.
  • Rodning CB, Erlandsen SL, Wilson ID, Carpenter AM (1982): Light microscopic morphometric analysis of rat ileal mucosa: II. Component quantitation of Paneth cells. Anat Rec, 204, 33-38.
  • Sandow MJ, Whitehead R (1979) : The Paneth cell. Gut, 20, 420-431.
  • Satoh Y, Ishikawa K, Ono K, Vollrath L (1986): Quantitative light microscopic observations on Paneth cells of germ-free and ex-germ-free Wistar rats. Digestion, 34, 115-121.
  • Satoh Y, Yamano M, Matsuda M, Ono K (1990): Ultrastructure of Paneth cells in the intestine of various mammals. J Electron Microsc Tech, 16, 69-80.
  • Satoh Y, Ono K, Moutairou K (1994): Paneth cells of African giant rats (Cricetomys gambianus). Acta Anat, 151, 49-53.
  • Sawada M, Nishikawa M, Adachi T, Midorikawa O, Hiai H (1993) : A paneth cell specific zinc-binding protein in the rat. Lab Invest, 68, 338-344.
  • Singh I (1971): The distribution of Paneth cells in the human small intestine. Anat Anz Bd, 128, 60-65.
  • Subbuswamy SG (1973) Paneth cells and goblet cells. J Pathol, 111, 181-189.
  • Sümbüloğlu K, Sümbüloğlu V (2000): Biyoistatistik. Hatipoğlu Yayınları, Ankara.
  • Takehana K, Masty J, Yamaguchi M, Kobayashi A, Yamada O, Kuroda M, Park YS, Iwasa K, Abe M (1998): Fine structural and histochemical study of equine Paneth cells. Anat Histol Embryol, 27, 125-129.
  • Taylor JJ, Flaa RC, Forks G (1964) : Histochemical analysis of Paneth cell granules in rats. Arch Path, 77, 278-285.
  • Trier JS (1966) : The Paneth cells: an enigma. Gastroenterology, 51, 560-562.
  • Veneable JH, Coggeshall R (1965) : A simplified lead citrate stain for use in electron microscopy. J Cell Biol, 25, 407-408.
  • Wehkamp J, Stange EF (2006): Paneth cells and the innate immune response. Curr Opin Gastroenterol, 22, 644- 650. Geliş tarihi: 10.03.2008 / Kabul tarihi: 01.05.2008
There are 41 citations in total.

Details

Primary Language Turkish
Subjects Veterinary Surgery
Other ID JA74ZE83TG
Journal Section Research Article
Authors

Emel Ergün

Levent Ergün

Asuman Özen

Aytül Kürüm

Publication Date March 1, 2009
Published in Issue Year 2009Volume: 56 Issue: 1

Cite

APA Ergün, E., Ergün, L., Özen, A., Kürüm, A. (2009). Ankara tavşanı ince bağırsağında Paneth hücrelerinin morfolojisi, histokimyası ve ince yapısı üzerinde çalışmalar. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 56(1), 25-30. https://doi.org/10.1501/Vetfak_0000002181
AMA Ergün E, Ergün L, Özen A, Kürüm A. Ankara tavşanı ince bağırsağında Paneth hücrelerinin morfolojisi, histokimyası ve ince yapısı üzerinde çalışmalar. Ankara Univ Vet Fak Derg. March 2009;56(1):25-30. doi:10.1501/Vetfak_0000002181
Chicago Ergün, Emel, Levent Ergün, Asuman Özen, and Aytül Kürüm. “Ankara tavşanı Ince bağırsağında Paneth hücrelerinin Morfolojisi, Histokimyası Ve Ince yapısı üzerinde çalışmalar”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 56, no. 1 (March 2009): 25-30. https://doi.org/10.1501/Vetfak_0000002181.
EndNote Ergün E, Ergün L, Özen A, Kürüm A (March 1, 2009) Ankara tavşanı ince bağırsağında Paneth hücrelerinin morfolojisi, histokimyası ve ince yapısı üzerinde çalışmalar. Ankara Üniversitesi Veteriner Fakültesi Dergisi 56 1 25–30.
IEEE E. Ergün, L. Ergün, A. Özen, and A. Kürüm, “Ankara tavşanı ince bağırsağında Paneth hücrelerinin morfolojisi, histokimyası ve ince yapısı üzerinde çalışmalar”, Ankara Univ Vet Fak Derg, vol. 56, no. 1, pp. 25–30, 2009, doi: 10.1501/Vetfak_0000002181.
ISNAD Ergün, Emel et al. “Ankara tavşanı Ince bağırsağında Paneth hücrelerinin Morfolojisi, Histokimyası Ve Ince yapısı üzerinde çalışmalar”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 56/1 (March 2009), 25-30. https://doi.org/10.1501/Vetfak_0000002181.
JAMA Ergün E, Ergün L, Özen A, Kürüm A. Ankara tavşanı ince bağırsağında Paneth hücrelerinin morfolojisi, histokimyası ve ince yapısı üzerinde çalışmalar. Ankara Univ Vet Fak Derg. 2009;56:25–30.
MLA Ergün, Emel et al. “Ankara tavşanı Ince bağırsağında Paneth hücrelerinin Morfolojisi, Histokimyası Ve Ince yapısı üzerinde çalışmalar”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 56, no. 1, 2009, pp. 25-30, doi:10.1501/Vetfak_0000002181.
Vancouver Ergün E, Ergün L, Özen A, Kürüm A. Ankara tavşanı ince bağırsağında Paneth hücrelerinin morfolojisi, histokimyası ve ince yapısı üzerinde çalışmalar. Ankara Univ Vet Fak Derg. 2009;56(1):25-30.

Cited By

Paneth cells in farm animals: current status and future direction
Journal of Animal Science and Biotechnology
https://doi.org/10.1186/s40104-023-00905-5