Anaplasma marginale msp1a geninin yeni bir tandem raporunu da içeren, Türkiye’nin Karaman yöresindeki sığırlarda Anaplasma ve Ehrlichia türlerinin moleküler araştırması
Abstract
Kene kaynaklı patojenler evcil ve yabani hayvanlarda ciddi sağlık problemlerine ve verim kaybına neden olur. Karaman ilindeki sığırlarda Anaplasma / Ehrlichia (A/E) ile enfeksiyon sıklığını saptamak için moleküler bir çalışma yapıldı. Venöz kan numuneleri görünüşte sağlıklı olan 150 sığırdan 2016 yılında alınmıştır. A/E türlerinin 16S rRNA geninin değişken V1 bölgesi amplifiye edildikten sonra tür spesifik problar kullanılarak reverse line blot (RLB) deneyi gerçekleştirilmiştir. Bazı örnekler sadece A/E probuna sinyal verdiğinden, örnekler Anaplasma marginale'nin major surface proteinleri (MSPs) açısından analiz edilmiştir. A. marginale'nin msp1α gen dizileri için genetik çeşitlilik ve tandem tekrar analizi yapıldı. Mikroskobik inceleme ile dört (%2.66) hayvanda Anaplasma benzeri cisimler tespit edildi. Anaplasma centrale RLB ile sekiz (%5.33) hayvanda tespit edildi. Örnekler semi-nested PZR ile A. marginale msp1a geni açısından incelediğinde, toplam dokuz (%6.00) hayvanın [6'sı (%4.00) RLB ile A. centrale açısından pozitif olan] A. marginale ile enfekte olduğu bulunmuştur. Ek olarak, MSP1a amplikonlarının dizileri bir tane yeni tandem tekrarı (Tr70) ortaya çıkardı. Bu sonuçlara göre Karaman ilindeki sığırlarda A. marginale ve A. centrale'nin bulunduğu tespit edilmiş ve bu çalışma bölgedeki sığırlarda bir yeni tandem tekrarı ile A. marginale’nin genetik çeşitliliğinin ilk kanıtını sağlamıştır.
Keywords
References
- Aktas M, Altay K, Dumanli N, et al. (2009): Molecular detection and identification of Ehrlichia and Anaplasma species in ixodid ticks. Parasitol Res, 104, 1243-1248.
- Aktas M, Altay K, Dumanli N (2011): Molecular detection and identification of Anaplasma and Ehrlichia species in cattle from Turkey. Ticks Tick-Borne Dis, 2, 62-65.
- Aktas M, Altay K, Ozubek S, et al. (2012): A survey of ixodid ticks feeding on cattle and prevalence of tick-borne pathogens in the Black Sea Region of Turkey. Vet Parasitol, 187, 567-571.
- Aktas M, Ozubek S (2017): Outbreak of anaplasmosis associated with novel genetic variants of Anaplasma marginale in a dairy cattle. Comp Immunol Microbiol Infect Dis, 54, 20-26.
- Aktas M, Vatansever Z, Altay K, et al. (2010): Molecular evidence for Anaplasma phagocytophilum in Ixodes ricinus from Turkey. Trans R Soc Trop Med Hyg, 104, 10-15.
- Aubry P, Geale DW (2011): A Review of Bovine Anaplasmosis. Transbound Emerg Dis, 58, 1-30.
- Aydin MF, Aktas M, Dumanli N (2013): Molecular identification of Theileria and Babesia in sheep and goats in the Black Sea Region in Turkey. Parasitol Res, 112, 2817-2824.
- Bekker CP, de Vos S, Taoufik A, et al. (2002): Simultaneus detection of Anaplasma and Ehrlichia species in ruminants and detection of Ehrlichia ruminantum in Amblyomma variegatum ticks by reverse line blot hybridization. Vet Microbiol, 89, 223-238.
- Birdane FM, Sevinc F, Derinbay O (2006): Anaplasma marginale infections in dairy cattle: clinical disease with high seroprevalence. Bull Vet Inst Pulawy, 50, 467-470.
- Cabezas-Cruz A, Passos LMF, Lis K, et al. (2013): Functional and immunological relevance of Anaplasma marginale major surface protein 1a sequence and structural analysis. PLoS ONE, 8:e65243.
- Catanese HN, Brayton KA, Gebremedhin AH (2016): Repeat Analyzer: a tool for analysing and managing short-sequence repeat data. BMC Genomics, 17, 422.
- Chen SM, Dumler JS, Bakken JS, et al. (1994): Identification of a granulocytotropic Ehrlichia species as the etiologic agent of human disease. J Clin Microbiol, 32, 589-595.
- De la Fuente J, Garcia-Garcia JC, Blouin EF, et al. (2001a): Evolution and function of tandem repeats in the major surface protein 1a of the ehrlichial pathogen Anaplasma marginale. Anim Health Res Rev, 2, 163-173.
- De la Fuente J, Garcia-Garcia JC, Blouin EF, et al. (2001b): Major surface protein 1a effects tick infection and transmission of Anaplasma marginale. Int J Parasitol, 31, 1705-1714.
- De la Fuente J, Ruybal P, Mtshali MS, et al. (2007): Analysis of world strains of Anaplasma marginale using major surface protein 1a repeat sequences. Vet Microbiol, 119, 382-390.
- Dumler JS, Choi K, Garcia-Garcia JC, et al. (2005): Human granulocytic anaplasmosis and Anaplasma phagocytophilum. Emerg Infect Dis, 11, 1828-1834.
- Estrada-Peña A, Naranjo V, Acevedo-Whitehouse K, et al. (2009): Phylogeographic analysis reveals association of tick-borne pathogen, Anaplasma marginale, MSP1α sequences with ecological traits affecting tick vector performance. BMC Biol, 57, 1-13.
- Fedorina EA, Arkhipova AL, Kosovskiy GY, et al. (2019): Molecular survey and genetic characterization of Anaplasma marginale isolates in cattle from two regions of Russia. Ticks Tick Borne Dis, 10, 251-257.
- Gokce HI, Genc O, Akca A, et al. (2008): Molecular and Serological Evidence of Anaplasma phagocytophilum Infection of Farm Animals in the Black Sea Region of Turkey. Acta Vet Hung, 56, 281-292.
- Guo S, Yuan Z, Wu G, et al. (2002): Epidemiology of ovine theileriosis in Ganan region, Gansu Province, China. Parasitol Res, 88, 36-37.
- Jongejan F, Uilenberg G (2004): The global importance of ticks. Parasitology, 129, 3-14.
- Kocan KM, Blouin EF, Barbet AF (2000): Anaplasmosis Control. Past, Present, and Future. Ann N Y Acad Sci, 916, 501-509.
- Lew AE, Bock RE, Minchin CM, et al. (2002): A msp1a polymerase chain reaction assay for specific detection and differentiation of Anaplasma marginale isolates. Vet Microbiol, 86, 325-335.
- Liu Q, Meli ML, Zhang Y, et al. (2016): Sequence heterogeneity in the 18S rRNA gene in Theileria equi from horses presented in Switzerland. Vet Parasitol, 221, 24-29.
- Pipano E, Krigel Y, Frank M, et al. (1986): Frozen Anaplasma centrale vaccine against anaplasmosis in cattle. Br Vet J, 142, 553-556.
- Schouls LM, Van De Pol I, Rijpkema SG, et al. (1999): Detection and identification of Ehrlichia, Borrelia burgdorferi sensu lato, and Bartonella species in Dutch Ixodes ricinus ticks. J Clin Microbiol, 37, 2215-2222.
- Silva JB, Fonseca AH (2014): Risk factors for anaplasmosis in dairy cows during the peripartum. Trop Anim Health Prod, 46, 461-465.
- Yang J, Han R, Liu Z, et al. (2017): Insight into the genetic diversity of Anaplasma marginale in cattle from ten provinces of China. Parasit Vectors, 10, 565.
- Yin H, Liu G, Luo J, et al. (2003): Observation on the schizont stage of an unidentified Theileria sp. in experimentally infected sheep. Parasitol Res, 91, 34-39.
Molecular survey of Anaplasma and Ehrlichia species in cattle from Karaman of Turkey, including a novel tandem report of Anaplasma marginale msp1a gene
Abstract
Tick-borne pathogens cause serious health problems and loss of productivity in domesticated and wild animals. A molecular study was performed to detect the frequency of infection with Anaplasma/Ehrlichia (A/E) in cattle from Karaman province of Turkey. Venous blood samples were taken from 150 apparently healthy cattle in 2016. After amplification the hypervariable V1 region of the 16S rRNA gene of A/E species, a reverse line blot (RLB) assay was performed using species-specific probes. Since some samples gave signal only to A/E catch-all probe, the samples analyzed in terms of major surface proteins (MSPs) of Anaplasma marginale. Genetic diversity and tandem repeat analysis were made for msp1α gene sequences of A. marginale. Anaplasma-like bodies were detected in four (2.66%) animals via microscopic examination. Anaplasma centrale was detected in eight (5.33%) animals via RLB. When the samples were examined in terms of A. marginale msp1a gene with semi-nested PCR, a total of nine (6.00%) animals [six of them (4.00%) were positive for A. centrale with RLB] were found to be infected with A. marginale. In addition, the sequences of MSP1a amplicons revealed one new tandem repeat (Tr70). According to these results, it was determined that A. marginale and A. centrale were found in cattle in Karaman province and this study provided the first evidence of genetic diversity of A. marginale with one new tandem repeat in cattle in the region.
Keywords
References
- Aktas M, Altay K, Dumanli N, et al. (2009): Molecular detection and identification of Ehrlichia and Anaplasma species in ixodid ticks. Parasitol Res, 104, 1243-1248.
- Aktas M, Altay K, Dumanli N (2011): Molecular detection and identification of Anaplasma and Ehrlichia species in cattle from Turkey. Ticks Tick-Borne Dis, 2, 62-65.
- Aktas M, Altay K, Ozubek S, et al. (2012): A survey of ixodid ticks feeding on cattle and prevalence of tick-borne pathogens in the Black Sea Region of Turkey. Vet Parasitol, 187, 567-571.
- Aktas M, Ozubek S (2017): Outbreak of anaplasmosis associated with novel genetic variants of Anaplasma marginale in a dairy cattle. Comp Immunol Microbiol Infect Dis, 54, 20-26.
- Aktas M, Vatansever Z, Altay K, et al. (2010): Molecular evidence for Anaplasma phagocytophilum in Ixodes ricinus from Turkey. Trans R Soc Trop Med Hyg, 104, 10-15.
- Aubry P, Geale DW (2011): A Review of Bovine Anaplasmosis. Transbound Emerg Dis, 58, 1-30.
- Aydin MF, Aktas M, Dumanli N (2013): Molecular identification of Theileria and Babesia in sheep and goats in the Black Sea Region in Turkey. Parasitol Res, 112, 2817-2824.
- Bekker CP, de Vos S, Taoufik A, et al. (2002): Simultaneus detection of Anaplasma and Ehrlichia species in ruminants and detection of Ehrlichia ruminantum in Amblyomma variegatum ticks by reverse line blot hybridization. Vet Microbiol, 89, 223-238.
- Birdane FM, Sevinc F, Derinbay O (2006): Anaplasma marginale infections in dairy cattle: clinical disease with high seroprevalence. Bull Vet Inst Pulawy, 50, 467-470.
- Cabezas-Cruz A, Passos LMF, Lis K, et al. (2013): Functional and immunological relevance of Anaplasma marginale major surface protein 1a sequence and structural analysis. PLoS ONE, 8:e65243.
- Catanese HN, Brayton KA, Gebremedhin AH (2016): Repeat Analyzer: a tool for analysing and managing short-sequence repeat data. BMC Genomics, 17, 422.
- Chen SM, Dumler JS, Bakken JS, et al. (1994): Identification of a granulocytotropic Ehrlichia species as the etiologic agent of human disease. J Clin Microbiol, 32, 589-595.
- De la Fuente J, Garcia-Garcia JC, Blouin EF, et al. (2001a): Evolution and function of tandem repeats in the major surface protein 1a of the ehrlichial pathogen Anaplasma marginale. Anim Health Res Rev, 2, 163-173.
- De la Fuente J, Garcia-Garcia JC, Blouin EF, et al. (2001b): Major surface protein 1a effects tick infection and transmission of Anaplasma marginale. Int J Parasitol, 31, 1705-1714.
- De la Fuente J, Ruybal P, Mtshali MS, et al. (2007): Analysis of world strains of Anaplasma marginale using major surface protein 1a repeat sequences. Vet Microbiol, 119, 382-390.
- Dumler JS, Choi K, Garcia-Garcia JC, et al. (2005): Human granulocytic anaplasmosis and Anaplasma phagocytophilum. Emerg Infect Dis, 11, 1828-1834.
- Estrada-Peña A, Naranjo V, Acevedo-Whitehouse K, et al. (2009): Phylogeographic analysis reveals association of tick-borne pathogen, Anaplasma marginale, MSP1α sequences with ecological traits affecting tick vector performance. BMC Biol, 57, 1-13.
- Fedorina EA, Arkhipova AL, Kosovskiy GY, et al. (2019): Molecular survey and genetic characterization of Anaplasma marginale isolates in cattle from two regions of Russia. Ticks Tick Borne Dis, 10, 251-257.
- Gokce HI, Genc O, Akca A, et al. (2008): Molecular and Serological Evidence of Anaplasma phagocytophilum Infection of Farm Animals in the Black Sea Region of Turkey. Acta Vet Hung, 56, 281-292.
- Guo S, Yuan Z, Wu G, et al. (2002): Epidemiology of ovine theileriosis in Ganan region, Gansu Province, China. Parasitol Res, 88, 36-37.
- Jongejan F, Uilenberg G (2004): The global importance of ticks. Parasitology, 129, 3-14.
- Kocan KM, Blouin EF, Barbet AF (2000): Anaplasmosis Control. Past, Present, and Future. Ann N Y Acad Sci, 916, 501-509.
- Lew AE, Bock RE, Minchin CM, et al. (2002): A msp1a polymerase chain reaction assay for specific detection and differentiation of Anaplasma marginale isolates. Vet Microbiol, 86, 325-335.
- Liu Q, Meli ML, Zhang Y, et al. (2016): Sequence heterogeneity in the 18S rRNA gene in Theileria equi from horses presented in Switzerland. Vet Parasitol, 221, 24-29.
- Pipano E, Krigel Y, Frank M, et al. (1986): Frozen Anaplasma centrale vaccine against anaplasmosis in cattle. Br Vet J, 142, 553-556.
- Schouls LM, Van De Pol I, Rijpkema SG, et al. (1999): Detection and identification of Ehrlichia, Borrelia burgdorferi sensu lato, and Bartonella species in Dutch Ixodes ricinus ticks. J Clin Microbiol, 37, 2215-2222.
- Silva JB, Fonseca AH (2014): Risk factors for anaplasmosis in dairy cows during the peripartum. Trop Anim Health Prod, 46, 461-465.
- Yang J, Han R, Liu Z, et al. (2017): Insight into the genetic diversity of Anaplasma marginale in cattle from ten provinces of China. Parasit Vectors, 10, 565.
- Yin H, Liu G, Luo J, et al. (2003): Observation on the schizont stage of an unidentified Theileria sp. in experimentally infected sheep. Parasitol Res, 91, 34-39.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery |
| Journal Section | Research Article |
| Authors | |
| Publication Date | June 14, 2019 |
| Published in Issue | Year 2019Volume: 66 Issue: 3 |