Abstract
The serum sialic acid concentration has been reported to be a potentially useful as tumour markers in many types of tumoural disease. The purpose of this study was to investigate of the levels of serum total sialic acid (TSA) and lipid-bound sialic acid (LSA) in transmissible venereal tumours (TVT) in Kangal Breed (Turkish Anatolian Shepherd Dog) bitches. Blood samples were collected from seven untreated bitches with TVT (test group) and eight healthy bitches (control group). Serum lipid bound sialic acid levels determination was done by the spectrophotometric procedure of Katopodis with resorcinol. Serum total sialic acid levels determination was done by the spectrophotometric procedure of Sydow. The mean TSA and LSA levels in the treated and control groups were 13.46±0.66 mg/dl (11.82-16.94) and 6.13±0.46 mg/dl (4.42-7.58); 9.15±0.59 mg/dl (6.28-11.48) and 2.26±0.24 (1.473.63) mg/dl, respectively. Both of serum levels of TSA and LSA in the untreated TVT dogs (n: 7) were significantly (p<0.001) higher than in the healthy dogs (n:8) The data revealed significant elevation in sialic acid levels in TVT and suggested potential utility of these parameters in diagnosis of the TVT. Results of this study suggest that TSA and LSA levels in sera may use in diagnosis serve as a valuable in these tumoural diseases
References
- Cylwik B, Chrostek L, Szmitkowski M (2005): Diagnostic value of total and lipid-bound sialic acid in malignancies. Pol Merkur Lekarski, 19, 237-241.
- Emmelot P (1973): Biochemical properties of normal and neoplastic cell surface. Eur J Cancer, 9, 319-333.
- Erunal-Maral N, Findik M, Aslan S (2000): Use of exfoliative cytology for diagnosis of transmissible venereal tumour and controlling the recovery period in the bitch. Deut Tierarztl Woch, 107, 175-180.
- Feldman EC, Nelson RW (1996): Canine female reproduction, 669. In: Canine and Feline Endocrinology and Reproduction, Feldman, EC (ed), WB Saunders, Philadelphia.
- Johnston SD, Kustritz MGV, Olson PN (2001): Disorders of the canine vagina, vestibule, and vulva, 239. In: Canine and Feline Theriogenology, Johnston, SD (ed), WB Saunders, Philadelphia.
- Katopodis N, Hirashut Y, Geller, N L, Stock J J (1982): Lipid associated sialic acid test for the detection of human cancer. Cancer Res, 42, 5270-5275.
- Kloppel TM, Franz C.P, Morre DJ, Richardson RC (1978): Serum sialic acid levels increased in tumor- bearing dogs. Am J Vet Res, 39, 1377-1380.
- Lagana A, Martinez, BP, Marino A, Fago G, Bizzari M (1995): Correlation of serum sialic acid fractions as markers for carcinoma of the uterine cervix. Anticancer Res, 15, 2341-2346.
- Madewell BR, Theilen, GH (1987): Skin tumours of mesenchymal origin, 282-309. In: Veterinary Cancer Medicine, Thelien GH, Madewell BR (eds), Lea &Febiger, Philadelphia.
- Manju V, Balasubramanian V, Nalini N (2002): Oxidative stress and tumour markers in cervical cancer patients. J Biochem Mol Biol, 6, 387-390.
- Noakes DE, Parkinson TJ, England GCW, Arthur GH (2001): Infertility in the bitch and queen 643. In: Arthur’s Veterinary Reproduction and Obstetrics, WB Saunders Edinburgh.
- Owen LN (1980): TNM Classification of Tumors in Domestic Animals World Health Organization, Geneva. VPH/CMO/80.20
- Paszkowska A, Berbec H, Semczuk A, Cybulski M (1998): Sialic acid concentration in serum and tissue of endometrial cancer patients. Eur J Obstet Gynecol Reprod Biol, 76, 211-215.
- Poli, A, Arispici M, Camillo F, Corazza M (1986): Increase of serum lipid-associated sialic acid concentration in dogs with neoplasm. Am J Vet Res 47, 607-609.
- Ros-Bullon MR, Sanchez-Pedreno P, Martinez-Liante JH (1999): Serum sialic acids in malignant melanoma patients and ROC curve analysis. Anticancer Res, 19, 3619-3622.
- Schauer R (1985): Sialic acids and their role as biological masks. Trends Biochem Sci ,10, 357-360.
- Schutter EM, Visser JJ, van Kamp GJ, Mensdorff- Pouilly S, van Dijk W, Hilgers J, Kenemans P (1992): The utility of lipid-associated sialic acid (LASA or LSA) as a serum marker for malignancy: a review of the literature. Tumour Biol, 13, 121-132
- Seyrek K, Seyrek-Intas K, Keskin A, Kargın-Kiral F, Musal B, Toplu N (2005): Biochemical and histochemical studies on the occurrence of sialic acids in mammary tumours of bitches. Rev Med Vet-Toulouse, 156, 258-263.
- Shamberger RJ (1984): Serum sialic acid in normals and in cancer patients. J Clin Biochem Nutr, 22, 647-651.
- Silver HK, Rangel DM, Morton DL (1978): Serum sialic acid elevations in malignant melanoma patients. Cancer. 41, 1497-1499.
- Sydow G (1980): A simplified quick method for determination of sialic acid in serum. Biomed Biochim Acta, 44 , 1721-1723.
- Thougaard AV, Jensen AL, Wenck A (1994): Evaluation of an automated spectrophotometric assay for the determination of total sialic acid in canine serum. Vet Res Commun, 18, 337-345.
- Thougaard AV, Hellmen E, Jensen AL (1998): Total serum sialic acid is a general disease marker rather than a specific tumour marker in dogs. Zentralbl Veterinarmed A, 45, 471-479
- Thougaard AV, Hellmen E, Pedersen HD, Jensen AL (1999): Correlation between alpha 1-acid glycoprotein and total sialic acid in serum from dogs with tumours. Zentralbl Veterinarmed A, 46, 231-237.
- Yick Fu W, Neale E, Wu Shun FW (1992): Serum lipid- associated sialic acid levels in gynecologic malignancies. Int J Gynecol Cancer, 3, 143-146. Geliş tarihi: 15.01.2007 / Kabul tarihi: 09.11.2007 Address for correspondence DVM. PhD Gunes Serin Adnan Menderes University Faculty of Veterinary Medicine Department of Obstetrics and Reproductive Diseases 09016 Isikli / Aydın / Turkey e-mail: guneserin@yahoo.com
Abstract
Çeşitli tümöral hastalıkların tanısı amacıyla serum sialik asit düzeyinden yararlanılmaktadır. Bu çalışmada Kangal ırkı dişi köpeklerde görülen bulaşıcı venereal tümör olgularınında serum TSA ve LSA düzeylerinin araştırılması amaçlanmıştır. Bu amaçla 7 tümörlü hastadan ve 8 sağlıklı köpekten kan örnekleri alındı. Serum lipide bağlı sialik asit düzeyleri Katopodis tarafından bildirilen resorsinol yöntemine göre ölçülürken serum total sialik asit düzeyler Sydow tarafından bildirilen yöntemle spektrofotometrik olarak ölçüldü. Test ve kontrol grubuna ait ortalama TSA ve LSA düzeyleri sırasıyla 13.46±0.66 mg/dl (11.8216.94) ve 6.13±0.46 mg/dl (4.42-7.58); 9.15±0.59 mg/dl (6.28-11.48) ile 2.26±0.24 (1.47-3.63) mg/dl bulunmuştur. Tümör grubunun (n=7) serum TSA ve LSA düzeyleri kontrol grubuna (n=8) oranla anlamlı ölçüde yüksek bulunmuştur. TVT olgularında sialik asit düzeyinin artış göstermesi tanı amaçlı olarak kullanılabilir. Sonuç olarak, serum TSA ve LSA düzeyleri TVT tanısında klinik muayene bulguları ile birlikte değerlendirilebileceği düşünülmektedir
References
- Cylwik B, Chrostek L, Szmitkowski M (2005): Diagnostic value of total and lipid-bound sialic acid in malignancies. Pol Merkur Lekarski, 19, 237-241.
- Emmelot P (1973): Biochemical properties of normal and neoplastic cell surface. Eur J Cancer, 9, 319-333.
- Erunal-Maral N, Findik M, Aslan S (2000): Use of exfoliative cytology for diagnosis of transmissible venereal tumour and controlling the recovery period in the bitch. Deut Tierarztl Woch, 107, 175-180.
- Feldman EC, Nelson RW (1996): Canine female reproduction, 669. In: Canine and Feline Endocrinology and Reproduction, Feldman, EC (ed), WB Saunders, Philadelphia.
- Johnston SD, Kustritz MGV, Olson PN (2001): Disorders of the canine vagina, vestibule, and vulva, 239. In: Canine and Feline Theriogenology, Johnston, SD (ed), WB Saunders, Philadelphia.
- Katopodis N, Hirashut Y, Geller, N L, Stock J J (1982): Lipid associated sialic acid test for the detection of human cancer. Cancer Res, 42, 5270-5275.
- Kloppel TM, Franz C.P, Morre DJ, Richardson RC (1978): Serum sialic acid levels increased in tumor- bearing dogs. Am J Vet Res, 39, 1377-1380.
- Lagana A, Martinez, BP, Marino A, Fago G, Bizzari M (1995): Correlation of serum sialic acid fractions as markers for carcinoma of the uterine cervix. Anticancer Res, 15, 2341-2346.
- Madewell BR, Theilen, GH (1987): Skin tumours of mesenchymal origin, 282-309. In: Veterinary Cancer Medicine, Thelien GH, Madewell BR (eds), Lea &Febiger, Philadelphia.
- Manju V, Balasubramanian V, Nalini N (2002): Oxidative stress and tumour markers in cervical cancer patients. J Biochem Mol Biol, 6, 387-390.
- Noakes DE, Parkinson TJ, England GCW, Arthur GH (2001): Infertility in the bitch and queen 643. In: Arthur’s Veterinary Reproduction and Obstetrics, WB Saunders Edinburgh.
- Owen LN (1980): TNM Classification of Tumors in Domestic Animals World Health Organization, Geneva. VPH/CMO/80.20
- Paszkowska A, Berbec H, Semczuk A, Cybulski M (1998): Sialic acid concentration in serum and tissue of endometrial cancer patients. Eur J Obstet Gynecol Reprod Biol, 76, 211-215.
- Poli, A, Arispici M, Camillo F, Corazza M (1986): Increase of serum lipid-associated sialic acid concentration in dogs with neoplasm. Am J Vet Res 47, 607-609.
- Ros-Bullon MR, Sanchez-Pedreno P, Martinez-Liante JH (1999): Serum sialic acids in malignant melanoma patients and ROC curve analysis. Anticancer Res, 19, 3619-3622.
- Schauer R (1985): Sialic acids and their role as biological masks. Trends Biochem Sci ,10, 357-360.
- Schutter EM, Visser JJ, van Kamp GJ, Mensdorff- Pouilly S, van Dijk W, Hilgers J, Kenemans P (1992): The utility of lipid-associated sialic acid (LASA or LSA) as a serum marker for malignancy: a review of the literature. Tumour Biol, 13, 121-132
- Seyrek K, Seyrek-Intas K, Keskin A, Kargın-Kiral F, Musal B, Toplu N (2005): Biochemical and histochemical studies on the occurrence of sialic acids in mammary tumours of bitches. Rev Med Vet-Toulouse, 156, 258-263.
- Shamberger RJ (1984): Serum sialic acid in normals and in cancer patients. J Clin Biochem Nutr, 22, 647-651.
- Silver HK, Rangel DM, Morton DL (1978): Serum sialic acid elevations in malignant melanoma patients. Cancer. 41, 1497-1499.
- Sydow G (1980): A simplified quick method for determination of sialic acid in serum. Biomed Biochim Acta, 44 , 1721-1723.
- Thougaard AV, Jensen AL, Wenck A (1994): Evaluation of an automated spectrophotometric assay for the determination of total sialic acid in canine serum. Vet Res Commun, 18, 337-345.
- Thougaard AV, Hellmen E, Jensen AL (1998): Total serum sialic acid is a general disease marker rather than a specific tumour marker in dogs. Zentralbl Veterinarmed A, 45, 471-479
- Thougaard AV, Hellmen E, Pedersen HD, Jensen AL (1999): Correlation between alpha 1-acid glycoprotein and total sialic acid in serum from dogs with tumours. Zentralbl Veterinarmed A, 46, 231-237.
- Yick Fu W, Neale E, Wu Shun FW (1992): Serum lipid- associated sialic acid levels in gynecologic malignancies. Int J Gynecol Cancer, 3, 143-146. Geliş tarihi: 15.01.2007 / Kabul tarihi: 09.11.2007 Address for correspondence DVM. PhD Gunes Serin Adnan Menderes University Faculty of Veterinary Medicine Department of Obstetrics and Reproductive Diseases 09016 Isikli / Aydın / Turkey e-mail: guneserin@yahoo.com
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery |
| Journal Section | Articles |
| Authors | |
| Publication Date | September 1, 2008 |
| Published in Issue | Year 2008Volume: 55 Issue: 2 |