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Histochemical characterization of glycoproteins in the gills of the carp

Year 2008, Volume: 55 Issue: 1, 61 - 64, 01.06.2008

Kenan Çınar Nurgül Şenol M. Rüştü Özen

https://doi.org/10.1501/Vetfak_0000000290

Abstract

The characteristics of the mucous cells located in gills of the fish Cyprinus carpio were investigated. Mucous cells were determined from gill arc, gill body, primary and secondary lamellae. Mostly mucous cells had oval-globular shape throughout all regions. Histochemical analysis of the gill of Cyprinus carpio showed that mucous content included glycogene and/or oxidizable dioles (PAS +), neutral or acid-rich (PAS/AB pH 2.5 +), sialic acid residues (KOH/PAS +) and strong acid sulphated (AF +) glycoproteins (GPs). Except these mucosubstances, carboxyl groups and/or with sulphate esters (AB pH 2.5 +) strong sulphate (AB pH 0.5 +) (AF/AB pH 2.5 +), O-sulphate esters (AB pH 1+) glycoproteins were also determined

Keywords

Cyprinus carpio density gill glycoproteins mucous cells

References

  • Arellano JM, Storch V, Sarasquete C (2004): Ultrastructural and histochemical study on gills and skin of the Senegal sole, Solea senegalensis. J Appl Ichthyol, 20, 452-460.
  • Bordas MA, Balebona MC, Chabrillon M, Rodriguez- Maroto JM, Morinigo MA (2003): Influence of temperature and salinity on the adhesion to mucous surfaces of gilt-head seabream (Sparus aurata L.) of pathogenic strains of Vibrio alginolyticus and Listonella anguillarum. B Eur Assoc Fish Pat, 23, 273-280.
  • Calabro C, Albanese MP, Lauriano ER, Martella S, Licata A (2005): Morphological, histochemical and immunohistochemical study of the gill epithelium in the abyssal teleost fish Coelorhynchus . Folia Histochem Cytobiol, 43, 51-56.
  • Carmona R, Garcia-Gallego M, Sanz A, Domezain A and Ostos-Garrido MV (2004): Chloride cells and pavement cells in gill epithelia of Acipenser naccarii: ultrastructural modifications in seawater-acclimated specimens. J Fish Biol, 64, 553-566.
  • Chabrillon M, Bordas MA, Morimigo MA, Balebona MC (2004): Kinetics of adhesion of Listonella anguillarum to the mucus of gilt-head sea bream, and the implication of surface components. Aquac Res, 35, 403-409.
  • Charles HJ, Terry AH (1997): Changes in gill morphology of atlantic salmon (Salmo salar) smolts due to addition of acid and aluminium to stream water. Environ Pollut, 97, 137-146.
  • Culling CFA, Reid PE, Dunn WL (1976): A new histochemical method for the identification and visualization of both side chain acylated and non-acylated sialic acids. J Histochem Cytochem, 24, 1225-1230.
  • Diaz AO, Garcia AM, Devincenti CV, Goldemberg AL (2001): Mucous cells in Micropogonias furnieri gills: histochemistry and ultrastructure. Anat Histol Embryol , 30, 135-139.
  • Diaz AO, Garcia AM, Devincenti CV, Goldemberg AL (2005): Ultrastructure and histochemical study of glycoconjugates in the gills of the White Croaker (Micropogonias furnieri). Anat Histol Embriyol, 34, 117-122.
  • Dunel EB, Sebest P, Chevalier C, Simon B, Bart HL (1996): Morphological changes indiced by acclimation high pressure in the gill epithelium of the freshwater Yellow Eel. J Fish Biol, 48, 1018-1022.
  • Dutar HM, Richmands CR, Zeno T (1993): Effect of diazinon on the gills of Bluegill Sunfish, Lepomis macrochirus J Environ Pathol Toxicol Oncol, 12, 219-227.
  • Ferguson HW, Morrison D, Ostland VE, Lumsden J, Byme P (1992) : Response of mucus-producing cell in gill disease of Rainbow trout (Oncorhynchus mykiss). J Comp Pathol, 106, 255-265.
  • Franklin GC (1990): Surface ultrastructural changes in the gills of Sockeye salmon (Teleostei: Oncorhynhus nerka) during seawater transfer: comparison of successful seawater adoptation. J Morphol, 206, 13-23.
  • Gomari G (1952): Gomari’ s aldehyde fuchsin stain. 238. In: Cellular Pathology Technique, CFA Culling, RT Allison, WT Barr (eds). Butterworths, London.
  • Herrler G, Rott R, Klenk HD, Muller HP, Shukla AK and Schauer R (1985): The receptor destroying enzyme of influenza C virus is neuraminate- O- acetyl esterase. Embo J, 4, 1503-1506.
  • Hilary M, Lease-Hansen-James A, Bergman-Harold L, Meyer-Joseph S (2003): Structural changes in gills of Lost River suckers exposed to elevated pH and ammonia concentrations. Comparative Biochemistry and Physiology Part C: Toxicol Pharmacol, 134, 491-500.
  • Jarial MS, Wilkins JH (2003): Ultrastructure of the external gill epithelium of the axolotl, Ambystoma mexicanum with reference to ionic transport. J Submicrosc Cytol Pathol, 35,445-455.
  • Jezierska B, Witeska M (2004): The effect of metals on fish gill functions-Gas and ion change (review). Fresenius Environ Bullet, 13, 1370-1378.
  • Laurent P, Perry SF (1990): Effect of cortisol on gill chloride cell morphology and ionic uptake in the freshwater trout, Salmo gairdneri. Cell Tissue Res, 259, 429-442.
  • Ledy K, Giamberini L, Pihan PC (2003): Mucous cell responces in gill and skin of brown trout Salmo trutta fario in acidic, aluminium containing stream water. Dis Aquat Organ, 56, 235-240.
  • Lev R, Spicer SS (1964): Specific staining of sulphate groups with alcian blue at low pH. J Histochem Cytochem, 12, 309.
  • Matei VE (1993): Changes in the cellular ultrastructure of the gill epithelium in Tilapia under the action of cadmium on the fish. Tsitol, 35, 34-41.
  • McManus JFA (1948): Histological and histochemical uses of periodic acid. Stain Technol, 23, 99-108.
  • Mittal AK, Fujimori O, Ueda T, Yamada K (1995): Carbohydrates in the epidermal mucous cells of a fresh- water fish Mastacembelus (Mastacembelidae, Pisces) as studied by electron-microscopic cytochemical methods. Cell Tissue Res, 280, 531-539.
  • Mowry RW (1956): Alcian blue techniques for the histochemical study of acidic carbohydrates. J Histochem Cytochem, 4, 407-408.
  • Munday BL, Zilberg D, Findlay V (2001): Gill disease of morine fish caused by infection with Neoporamoeba pemaguidensis. J Fish Dis, 24, 497-507.
  • Park CM, Reid PE, Owen DA, Volz D, Dunn WL (1987): Histochemical studies of epithelial cell glycoproteins in normal rat colon. Histochem J, 19, 546-554.
  • Pfeiffer CJ, Smith BJ, Smith SA (1999): Ultrastructural morphology of the gill of the hybrid striped bass (Morone saxatilis X M. chrysops). Anat Histol Embryol 28, 337- 344.
  • Playle RC, Wood CM (1991): Mechanisms of aluminium extraction and accumilation at the gills of rainbow trout, Oncorhynchus mykiss (Walbaum), in acidic soft water. J Fish Biol, 38, 791-805.
  • Powell MD, Porsons HJ, Nowak BF (2001)
  • Physiological effects of freshwater bathing of Atlantic salmon (Salmo salar) as a treatment for amoebic gill disease. Aquaculture,199,259-266.
  • Sabaoia-Moraes SMT, Hernandez-Blazquez FJ, Mota DL, Bittencourt AM (1996): Mucous cell rypes in branchial epithelium of the euryhaline fish Poecilia vivipara. J Fish Biol, 49, 549-558.
  • Sarasquete C, Canales MLG, Arellano J, Cueto JAM, Ribeiro L, Dinis MT (1998): Histochemical study of skin and gills of Senegal solea, Solea senegalensis larvae and adults. Histol Histopathol, 13, 727-735.
  • Shane DR, Powell MD (2003): Comparative ionic flux and gill mucous cell histochemistry: effects of salinitiy and disease status in Atlantic salmon (Salmo salar L.). Comp Biochem Physiol Part A: Molecular and Integrative Physiology, 134, 525-537.
  • Spicer SS, Meyer DB (1960): Aldehyde fuchsin / alcian blue. 233. In: Cellular Pathology Technique, CFA Culling, RT Allison, WT Barr (eds). Butterworths, London.
  • Suprasert A, Fujioka T, Yamada K (1987): The histochemistry of glycoconjugates in the colonic epithelium of the chicken. Histochemistry, 86, 491-497.
  • Whitear M (1986): The skin of fishes including cyslostomes epidermis. 8-38. In: Biology of the Integument, Vol. 2. Vertebrates, J Bereiter-Hahn, AG Matoltsy, KS Richards (eds). Springer-Verlag, Berlin.
  • Wilson RW, Bergman HL, Wood CM (1994): Metabolic costs and physiological consequences of acclimation to aluminium in juvenile rainbow trout, Oncorhynchus mykiss (Walbaum), gill morphology, swimming performance and aerobic scope. Can Fish Aquat Sci, 51, 536-544. Geliş tarihi: 05.02.2007 / Kabul tarihi: 16.05.2007 Address for correspondance Dr. Kenan Çınar Department of Biology Faculty of Arts and Sciences Süleyman Demirel University 32260, Isparta, Turkey e-mail: kcinar@fef.sdu.edu.tr

Sazan (Cyprinus carpio) solungaç glikoproteinlerinin histokimyasal özellikleri

Year 2008, Volume: 55 Issue: 1, 61 - 64, 01.06.2008

Kenan Çınar Nurgül Şenol M. Rüştü Özen

https://doi.org/10.1501/Vetfak_0000000290

Abstract

Cyprinus carpio solungaçlarında yer alan karakteristik mukus hücreleri incelendi. Mukus hücreleri solungaç arklarında, solungaç lamellerinde, primer ve sekonder lamellerde tespit edildi. Tüm bölgeler boyunca mukus hücrelerinin çoğu oval-yuvarlak bir şekle sahipti. Histokimyasal analizler solungaçlardaki mukus hücrelerinin glikojen ya da oksidizable diolleri (PAS +), nötral ya da asitce zengin (PAS/AB pH 2.5+), siyalik asid rezidulleri (KOH/PAS +) ve asitce zengin sulfatlı (AF +) glikoproteinleri içerdiği gösterildi. Bu mukosubstanslar dışında karboksil gruplu ya da sülfat esterli (AB pH 2.5 +) güçlü sülfatlı (AB pH 0.5 +) (AF/AB pH 2.5 +), O-sülfat esterli (AB pH 1+) glikoproteinler tespit edildi

Keywords

Cyprinus carpio glikoproteinler mukus hücreleri solungaç yoğunluk

References

  • Arellano JM, Storch V, Sarasquete C (2004): Ultrastructural and histochemical study on gills and skin of the Senegal sole, Solea senegalensis. J Appl Ichthyol, 20, 452-460.
  • Bordas MA, Balebona MC, Chabrillon M, Rodriguez- Maroto JM, Morinigo MA (2003): Influence of temperature and salinity on the adhesion to mucous surfaces of gilt-head seabream (Sparus aurata L.) of pathogenic strains of Vibrio alginolyticus and Listonella anguillarum. B Eur Assoc Fish Pat, 23, 273-280.
  • Calabro C, Albanese MP, Lauriano ER, Martella S, Licata A (2005): Morphological, histochemical and immunohistochemical study of the gill epithelium in the abyssal teleost fish Coelorhynchus . Folia Histochem Cytobiol, 43, 51-56.
  • Carmona R, Garcia-Gallego M, Sanz A, Domezain A and Ostos-Garrido MV (2004): Chloride cells and pavement cells in gill epithelia of Acipenser naccarii: ultrastructural modifications in seawater-acclimated specimens. J Fish Biol, 64, 553-566.
  • Chabrillon M, Bordas MA, Morimigo MA, Balebona MC (2004): Kinetics of adhesion of Listonella anguillarum to the mucus of gilt-head sea bream, and the implication of surface components. Aquac Res, 35, 403-409.
  • Charles HJ, Terry AH (1997): Changes in gill morphology of atlantic salmon (Salmo salar) smolts due to addition of acid and aluminium to stream water. Environ Pollut, 97, 137-146.
  • Culling CFA, Reid PE, Dunn WL (1976): A new histochemical method for the identification and visualization of both side chain acylated and non-acylated sialic acids. J Histochem Cytochem, 24, 1225-1230.
  • Diaz AO, Garcia AM, Devincenti CV, Goldemberg AL (2001): Mucous cells in Micropogonias furnieri gills: histochemistry and ultrastructure. Anat Histol Embryol , 30, 135-139.
  • Diaz AO, Garcia AM, Devincenti CV, Goldemberg AL (2005): Ultrastructure and histochemical study of glycoconjugates in the gills of the White Croaker (Micropogonias furnieri). Anat Histol Embriyol, 34, 117-122.
  • Dunel EB, Sebest P, Chevalier C, Simon B, Bart HL (1996): Morphological changes indiced by acclimation high pressure in the gill epithelium of the freshwater Yellow Eel. J Fish Biol, 48, 1018-1022.
  • Dutar HM, Richmands CR, Zeno T (1993): Effect of diazinon on the gills of Bluegill Sunfish, Lepomis macrochirus J Environ Pathol Toxicol Oncol, 12, 219-227.
  • Ferguson HW, Morrison D, Ostland VE, Lumsden J, Byme P (1992) : Response of mucus-producing cell in gill disease of Rainbow trout (Oncorhynchus mykiss). J Comp Pathol, 106, 255-265.
  • Franklin GC (1990): Surface ultrastructural changes in the gills of Sockeye salmon (Teleostei: Oncorhynhus nerka) during seawater transfer: comparison of successful seawater adoptation. J Morphol, 206, 13-23.
  • Gomari G (1952): Gomari’ s aldehyde fuchsin stain. 238. In: Cellular Pathology Technique, CFA Culling, RT Allison, WT Barr (eds). Butterworths, London.
  • Herrler G, Rott R, Klenk HD, Muller HP, Shukla AK and Schauer R (1985): The receptor destroying enzyme of influenza C virus is neuraminate- O- acetyl esterase. Embo J, 4, 1503-1506.
  • Hilary M, Lease-Hansen-James A, Bergman-Harold L, Meyer-Joseph S (2003): Structural changes in gills of Lost River suckers exposed to elevated pH and ammonia concentrations. Comparative Biochemistry and Physiology Part C: Toxicol Pharmacol, 134, 491-500.
  • Jarial MS, Wilkins JH (2003): Ultrastructure of the external gill epithelium of the axolotl, Ambystoma mexicanum with reference to ionic transport. J Submicrosc Cytol Pathol, 35,445-455.
  • Jezierska B, Witeska M (2004): The effect of metals on fish gill functions-Gas and ion change (review). Fresenius Environ Bullet, 13, 1370-1378.
  • Laurent P, Perry SF (1990): Effect of cortisol on gill chloride cell morphology and ionic uptake in the freshwater trout, Salmo gairdneri. Cell Tissue Res, 259, 429-442.
  • Ledy K, Giamberini L, Pihan PC (2003): Mucous cell responces in gill and skin of brown trout Salmo trutta fario in acidic, aluminium containing stream water. Dis Aquat Organ, 56, 235-240.
  • Lev R, Spicer SS (1964): Specific staining of sulphate groups with alcian blue at low pH. J Histochem Cytochem, 12, 309.
  • Matei VE (1993): Changes in the cellular ultrastructure of the gill epithelium in Tilapia under the action of cadmium on the fish. Tsitol, 35, 34-41.
  • McManus JFA (1948): Histological and histochemical uses of periodic acid. Stain Technol, 23, 99-108.
  • Mittal AK, Fujimori O, Ueda T, Yamada K (1995): Carbohydrates in the epidermal mucous cells of a fresh- water fish Mastacembelus (Mastacembelidae, Pisces) as studied by electron-microscopic cytochemical methods. Cell Tissue Res, 280, 531-539.
  • Mowry RW (1956): Alcian blue techniques for the histochemical study of acidic carbohydrates. J Histochem Cytochem, 4, 407-408.
  • Munday BL, Zilberg D, Findlay V (2001): Gill disease of morine fish caused by infection with Neoporamoeba pemaguidensis. J Fish Dis, 24, 497-507.
  • Park CM, Reid PE, Owen DA, Volz D, Dunn WL (1987): Histochemical studies of epithelial cell glycoproteins in normal rat colon. Histochem J, 19, 546-554.
  • Pfeiffer CJ, Smith BJ, Smith SA (1999): Ultrastructural morphology of the gill of the hybrid striped bass (Morone saxatilis X M. chrysops). Anat Histol Embryol 28, 337- 344.
  • Playle RC, Wood CM (1991): Mechanisms of aluminium extraction and accumilation at the gills of rainbow trout, Oncorhynchus mykiss (Walbaum), in acidic soft water. J Fish Biol, 38, 791-805.
  • Powell MD, Porsons HJ, Nowak BF (2001)
  • Physiological effects of freshwater bathing of Atlantic salmon (Salmo salar) as a treatment for amoebic gill disease. Aquaculture,199,259-266.
  • Sabaoia-Moraes SMT, Hernandez-Blazquez FJ, Mota DL, Bittencourt AM (1996): Mucous cell rypes in branchial epithelium of the euryhaline fish Poecilia vivipara. J Fish Biol, 49, 549-558.
  • Sarasquete C, Canales MLG, Arellano J, Cueto JAM, Ribeiro L, Dinis MT (1998): Histochemical study of skin and gills of Senegal solea, Solea senegalensis larvae and adults. Histol Histopathol, 13, 727-735.
  • Shane DR, Powell MD (2003): Comparative ionic flux and gill mucous cell histochemistry: effects of salinitiy and disease status in Atlantic salmon (Salmo salar L.). Comp Biochem Physiol Part A: Molecular and Integrative Physiology, 134, 525-537.
  • Spicer SS, Meyer DB (1960): Aldehyde fuchsin / alcian blue. 233. In: Cellular Pathology Technique, CFA Culling, RT Allison, WT Barr (eds). Butterworths, London.
  • Suprasert A, Fujioka T, Yamada K (1987): The histochemistry of glycoconjugates in the colonic epithelium of the chicken. Histochemistry, 86, 491-497.
  • Whitear M (1986): The skin of fishes including cyslostomes epidermis. 8-38. In: Biology of the Integument, Vol. 2. Vertebrates, J Bereiter-Hahn, AG Matoltsy, KS Richards (eds). Springer-Verlag, Berlin.
  • Wilson RW, Bergman HL, Wood CM (1994): Metabolic costs and physiological consequences of acclimation to aluminium in juvenile rainbow trout, Oncorhynchus mykiss (Walbaum), gill morphology, swimming performance and aerobic scope. Can Fish Aquat Sci, 51, 536-544. Geliş tarihi: 05.02.2007 / Kabul tarihi: 16.05.2007 Address for correspondance Dr. Kenan Çınar Department of Biology Faculty of Arts and Sciences Süleyman Demirel University 32260, Isparta, Turkey e-mail: kcinar@fef.sdu.edu.tr
There are 38 citations in total.

Details

Primary Language English
Subjects Veterinary Surgery
Journal Section Research Article
Authors

Kenan Çınar

Nurgül Şenol

M. Rüştü Özen

Publication Date June 1, 2008
Published in Issue Year 2008Volume: 55 Issue: 1

Cite

APA Çınar, K., Şenol, N., & Özen, M. R. (2008). Histochemical characterization of glycoproteins in the gills of the carp. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 55(1), 61-64. https://doi.org/10.1501/Vetfak_0000000290
AMA Çınar K, Şenol N, Özen MR. Histochemical characterization of glycoproteins in the gills of the carp. Ankara Univ Vet Fak Derg. June 2008;55(1):61-64. doi:10.1501/Vetfak_0000000290
Chicago Çınar, Kenan, Nurgül Şenol, and M. Rüştü Özen. “Histochemical Characterization of Glycoproteins in the Gills of the Carp”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 55, no. 1 (June 2008): 61-64. https://doi.org/10.1501/Vetfak_0000000290.
EndNote Çınar K, Şenol N, Özen MR (June 1, 2008) Histochemical characterization of glycoproteins in the gills of the carp. Ankara Üniversitesi Veteriner Fakültesi Dergisi 55 1 61–64.
IEEE K. Çınar, N. Şenol, and M. R. Özen, “Histochemical characterization of glycoproteins in the gills of the carp”, Ankara Univ Vet Fak Derg, vol. 55, no. 1, pp. 61–64, 2008, doi: 10.1501/Vetfak_0000000290.
ISNAD Çınar, Kenan et al. “Histochemical Characterization of Glycoproteins in the Gills of the Carp”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 55/1 (June 2008), 61-64. https://doi.org/10.1501/Vetfak_0000000290.
JAMA Çınar K, Şenol N, Özen MR. Histochemical characterization of glycoproteins in the gills of the carp. Ankara Univ Vet Fak Derg. 2008;55:61–64.
MLA Çınar, Kenan et al. “Histochemical Characterization of Glycoproteins in the Gills of the Carp”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 55, no. 1, 2008, pp. 61-64, doi:10.1501/Vetfak_0000000290.
Vancouver Çınar K, Şenol N, Özen MR. Histochemical characterization of glycoproteins in the gills of the carp. Ankara Univ Vet Fak Derg. 2008;55(1):61-4.