Abstract
This study was carried out to determine the prevalence of ovine Sarcocystis species in Izmir province. A total of 734 muscle samples of 200 sheep from four different age groups were surveyed by the digestion and histological examination methods for the presence of Sarcocystis species. Sarcocystis ovifelis, Sarcocystis ovicanis, and Sarcocystis arieticanis were detected as the responsible species for ovine sarcosporidiosis in Izmir province. Macrocysts of S.ovifelis were found in 24.5 % (49/200) of sheep and the highest prevalence was detected in the oesophagus in all age groups. Microcysts of S.ovicanis and S.arieticanis were present in 86.5 % (173/200) of sheep. The prevalence of Sarcocystis cysts increased significantly with the age. Although microcysts were detected in 46 % (23/50) of lambs up to 6 months of age, they were present in all muscle samples of sheep (150/150) over 6 months of age. Histologically, no inflammatory reaction was seen against sarcocysts within the infected muscles, unless they degenerated. There was no significant difference (p>0.05) statistically between sensitivities of two diagnostic methods (digestion and histology) in detecting Sarcocystis species. These results reveal the fact that the environment is heavily contaminated with sporocysts, and the ingestion of sporocysts by sheep begins from the young ages in Izmir province
Keywords
References
- Abo-Shehada MN (1996): Age variations in the prevalence of sarcocystosis in sheep and goats from northern and central Jordan. Prev Vet Med, 27, 135-140.
- Britt DP, Baker JR (1990): Causes of death and illness in the native sheep of North Ronaldsay, Orkney. I. Adult sheep. Brit Vet J, 146, 129-142.
- Collins GH, Atkinson E, Charleston WAG (1979): Studies on sarcocystis species. III. The macrocystic species of sheep. N Z Vet J, 27, 204-206.
- Dubey JP, Lindsay DS, Speer CA, Fayer R , Livingston CW (1988): Sarcocystis arieticanis and other Sarcocystis species in sheep in the United States. J Parasitol, 74, 1033- 1038.
- Dubey JP, Speer CA, Fayer R (1989): Sarcocystosis of Animals and Man. CRC Press Inc, Boca Raton, Florida.
- Egger A (1994): Sarkosporidien beim Schlachtschaf in Osttirol. Wien Tierarztl Mschr, 81, 247.
- Erber M (1977): Möglichkeiten des Nachweisses und der Differenzierung von Zwei Sarcocystis-Arten des Schweines. Berl Münch Tierarztl Wochenschr, 90, 480-482.
- Göksu K (1975): Koyunlarda sarcocystosis'in yayılışı üzerinde araştırmalar. İstanbul Üniv Vet Fak Derg, 1, 110-127.
- Kesici T, Kocabaş Z (1998): Biyoistatistik. Ankara Üniversitesi Basımevi, Ankara Üniv Eczacılık Fak Yay No. 79.
- Kudi AC, Aganga AO, Ogbogu VC, Umoh JU (1991): Prevalence of sarcocystis species in sheep and goats in Northern Nigeria. Rev Elev Med Vet Pays Trop, 44, 59-60.
- Latif BMA, Al-Delemi JK, Mohammed BS, Al-Bayati, SM, Al-Amiry AM (1999): Prevalence of Sarcocystis spp. in meat-producing animals in Iraq. Vet Parasitol, 84, 85-90.
- Mal’a P, Baranova, M (1995): Diagnosis of sarcocystis infection in slaughtered animals at veterinary meat inspection. Vet Med-Czech, 40, 97-100.
- Martinez-Moreno A, Moreno-Montanez T, Martinez- Gomez F, Hernandez-Rodriguez S, Martinez-Cruz S (1989): Prevalence of ovine sarcocystosis in Cordoba, Spain. Rev Iber Parasitol, 49, 283-285.
- Maskar U, Özder M, Dikmen S (1971): Çeşitli kasaplık hayvan türleri ile et müstahzaratlarında sarkosporidi bakımından histolojik araştırma. Mikrobiol Derg, 24, 86- 104.
- Munday BL (1978): Cats as definitive hosts for sarcocystis of sheep. N Z Vet J, 26, 166.
- Okur H, Kandemir O, Şahin İ (1995): An investigation on sarcocystis species in the cattle and the sheep in Bayburt. T Parazitol Derg, 19, 113-118.
- Oryan A, Moghaddar N, Gaur SNS (1996): The distribution pattern of sarcocystis species, their transmission and pathogenesis in sheep in Fars Province of Iran. Vet Res Commun, 20, 243-253.
- O’Toole D (1987): Experimental ovine sarcocystosis: sequential ultrastructural pathology in skeletal muscle. J Comp Pathol, 97, 51-60.
- O’Toole D, Duffell SJ, Upcott DH, Frewin D (1986): Experimental microcyst sarcocystis infection in lambs: pathology. Vet Rec, 119, 525-531.
- Öztürk G, Küçükerden N (1996): Elazığ Et ve Balık Kurumu mezbahasında kesilen koyunlarda sarcocystis türlerinin yayılışı. Etlik Vet Mikrob Derg, 8, 74-83.
- Reztlaff N (1972): Über das Vorkommen von Sarkosporidien bei schlachtschafen und schlachtziegender Türkei. Schlacht Viehhof Ztg, 72, 192-196.
- Saito M, Shibata Y, Itagaki H (1996): Sarcocystis arieticanis of sheep in Japan (Protozoa:Apicomplexa). Jpn J Parasitol, 45, 290-294.
- Savini G, Dunsmore JD, Robertson ID, Seneviratna P (1993): Sarcocystis spp in Western Australian sheep. Aust Vet J, 70, 152-154.
- Schmidtova D, Jurasek V (1992): Seasonal dynamics of the excretion of sarcocystis sporocysts in the faeces of dogs in relation to the incidence of sarcocystosis in sheep. Veterinastvi, 42, 48-49.
- Seneviratna P, Edward AG, DeGiusti DL (1975): Frequency of Sarcocystis spp. Detroit, Metropolitan area, Michigan. Am J Vet Res, 36, 337-339.
- Sevinç F, Altınöz F, Uslu U, Aldemir OS (2000): Koyunlarda sarcocystis türlerinin yaygınlığı. Vet Bil Derg, 16, 75-79.
- Svobodova V, Nevole M (1990): Use of the muscle digestion method and indirect immunofluorescence reaction in the diagnosis of sarcocystosis in sheep. Acta Vet Brno, 59, 157-170.
- Taşçı S, Değer S (1989): Van mezbahasında kesilen koyunlarda sarcocystosis'in yayılışı. Ankara Üniv Vet Fak Derg, 36, 540-552.
- Tüzer E, Demir S (1987): Bursa yöresinde koyunlarda sarkosporidiozis. İstanbul Üniv Vet Fak Derg, 13, 12-20.
Abstract
Bu çalışma, İzmir ilinde koyun Sarcocystis türlerinin yayılışını belirlemek amacıyla yapıldı. Bu amaçla dört farklı yaş grubundan 200 koyuna ait toplam 734 kas örneği, Sarcocystis türlerinin varlığı yönünden enzim tekniği ve histolojik muayene metodları ile araştırıldı. İzmir ilindeki koyunlarda sarcosporidiosis’den sorumlu türler, S.ovifelis, S.ovicanis ve S.arieticanis olarak tespit edildi. S.ovifelis makrokistleri, koyunların % 24,5 (49/200)'inde bulundu ve tüm yaş grublarında en yüksek prevalans ösefagusta tespit edildi. S.ovicanis ve S.arieticanis mikrokistleri, koyunların % 86,5 (173/200)’inde mevcuttu. Sarcocystis kistlerinin prevalansı, yaş ile önemli derecede arttı. Mikrokistler, 6 aylığa kadar olan kuzuların % 46 (23/50)’sında bulunduğu halde, 6 aylıktan büyük koyunların tüm kas örneklerinde mevcuttu. Histolojik olarak, kistler dejenere olmadıkça, enfekte kas tellerindeki sarkokistlere karşı yangısal bir reaksiyon görülmedi. Sarcocystis türlerinin tespitinde, iki teşhis metodunun (enzim tekniği ve histoloji) duyarlılıkları arasında istatistiksel olarak önemli bir fark (p>0,05) bulunmadı. Bu sonuçlar, İzmir ilinde çevrenin sporokistler ile yoğun şekilde kontamine olduğu ve koyunlar tarafından sporokist alımının genç yaşlardan itibaren başladığı gerçeğini ortaya koymaktadır
Keywords
References
- Abo-Shehada MN (1996): Age variations in the prevalence of sarcocystosis in sheep and goats from northern and central Jordan. Prev Vet Med, 27, 135-140.
- Britt DP, Baker JR (1990): Causes of death and illness in the native sheep of North Ronaldsay, Orkney. I. Adult sheep. Brit Vet J, 146, 129-142.
- Collins GH, Atkinson E, Charleston WAG (1979): Studies on sarcocystis species. III. The macrocystic species of sheep. N Z Vet J, 27, 204-206.
- Dubey JP, Lindsay DS, Speer CA, Fayer R , Livingston CW (1988): Sarcocystis arieticanis and other Sarcocystis species in sheep in the United States. J Parasitol, 74, 1033- 1038.
- Dubey JP, Speer CA, Fayer R (1989): Sarcocystosis of Animals and Man. CRC Press Inc, Boca Raton, Florida.
- Egger A (1994): Sarkosporidien beim Schlachtschaf in Osttirol. Wien Tierarztl Mschr, 81, 247.
- Erber M (1977): Möglichkeiten des Nachweisses und der Differenzierung von Zwei Sarcocystis-Arten des Schweines. Berl Münch Tierarztl Wochenschr, 90, 480-482.
- Göksu K (1975): Koyunlarda sarcocystosis'in yayılışı üzerinde araştırmalar. İstanbul Üniv Vet Fak Derg, 1, 110-127.
- Kesici T, Kocabaş Z (1998): Biyoistatistik. Ankara Üniversitesi Basımevi, Ankara Üniv Eczacılık Fak Yay No. 79.
- Kudi AC, Aganga AO, Ogbogu VC, Umoh JU (1991): Prevalence of sarcocystis species in sheep and goats in Northern Nigeria. Rev Elev Med Vet Pays Trop, 44, 59-60.
- Latif BMA, Al-Delemi JK, Mohammed BS, Al-Bayati, SM, Al-Amiry AM (1999): Prevalence of Sarcocystis spp. in meat-producing animals in Iraq. Vet Parasitol, 84, 85-90.
- Mal’a P, Baranova, M (1995): Diagnosis of sarcocystis infection in slaughtered animals at veterinary meat inspection. Vet Med-Czech, 40, 97-100.
- Martinez-Moreno A, Moreno-Montanez T, Martinez- Gomez F, Hernandez-Rodriguez S, Martinez-Cruz S (1989): Prevalence of ovine sarcocystosis in Cordoba, Spain. Rev Iber Parasitol, 49, 283-285.
- Maskar U, Özder M, Dikmen S (1971): Çeşitli kasaplık hayvan türleri ile et müstahzaratlarında sarkosporidi bakımından histolojik araştırma. Mikrobiol Derg, 24, 86- 104.
- Munday BL (1978): Cats as definitive hosts for sarcocystis of sheep. N Z Vet J, 26, 166.
- Okur H, Kandemir O, Şahin İ (1995): An investigation on sarcocystis species in the cattle and the sheep in Bayburt. T Parazitol Derg, 19, 113-118.
- Oryan A, Moghaddar N, Gaur SNS (1996): The distribution pattern of sarcocystis species, their transmission and pathogenesis in sheep in Fars Province of Iran. Vet Res Commun, 20, 243-253.
- O’Toole D (1987): Experimental ovine sarcocystosis: sequential ultrastructural pathology in skeletal muscle. J Comp Pathol, 97, 51-60.
- O’Toole D, Duffell SJ, Upcott DH, Frewin D (1986): Experimental microcyst sarcocystis infection in lambs: pathology. Vet Rec, 119, 525-531.
- Öztürk G, Küçükerden N (1996): Elazığ Et ve Balık Kurumu mezbahasında kesilen koyunlarda sarcocystis türlerinin yayılışı. Etlik Vet Mikrob Derg, 8, 74-83.
- Reztlaff N (1972): Über das Vorkommen von Sarkosporidien bei schlachtschafen und schlachtziegender Türkei. Schlacht Viehhof Ztg, 72, 192-196.
- Saito M, Shibata Y, Itagaki H (1996): Sarcocystis arieticanis of sheep in Japan (Protozoa:Apicomplexa). Jpn J Parasitol, 45, 290-294.
- Savini G, Dunsmore JD, Robertson ID, Seneviratna P (1993): Sarcocystis spp in Western Australian sheep. Aust Vet J, 70, 152-154.
- Schmidtova D, Jurasek V (1992): Seasonal dynamics of the excretion of sarcocystis sporocysts in the faeces of dogs in relation to the incidence of sarcocystosis in sheep. Veterinastvi, 42, 48-49.
- Seneviratna P, Edward AG, DeGiusti DL (1975): Frequency of Sarcocystis spp. Detroit, Metropolitan area, Michigan. Am J Vet Res, 36, 337-339.
- Sevinç F, Altınöz F, Uslu U, Aldemir OS (2000): Koyunlarda sarcocystis türlerinin yaygınlığı. Vet Bil Derg, 16, 75-79.
- Svobodova V, Nevole M (1990): Use of the muscle digestion method and indirect immunofluorescence reaction in the diagnosis of sarcocystosis in sheep. Acta Vet Brno, 59, 157-170.
- Taşçı S, Değer S (1989): Van mezbahasında kesilen koyunlarda sarcocystosis'in yayılışı. Ankara Üniv Vet Fak Derg, 36, 540-552.
- Tüzer E, Demir S (1987): Bursa yöresinde koyunlarda sarkosporidiozis. İstanbul Üniv Vet Fak Derg, 13, 12-20.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery |
| Journal Section | Research Article |
| Authors | |
| Publication Date | June 1, 2007 |
| Published in Issue | Year 2007Volume: 54 Issue: 2 |