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Determination of zeranol residues and the serum testosterone oestrogene and progesterone levels in lambs around Ankara region

Year 2007, Volume: 54 Issue: 1, 7 - 10, 01.03.2007

Güzin Özkurt Borazan Hilal Karagül Sefa Çelik Nil Ünal Mert Pekcan Tevhide Sel

https://doi.org/10.1501/Vetfak_0000000247
Cited By: 1

Abstract

Zeranol is a synthetic derivative of Zearalenone which has been used as an anabolic substance in sheep and cattle to increase growth of zeranol in food producing animals. The usage of zeranol is prohibited in most countries of the European Union and in Turkey. In the illegal use of zeranol it is difficult to determine its presence because the amount of zeranol given and period is not known. As well as this, the age and breed of the animal plus individual variations affect the period of removal of zeranol from the body. Because of this rapid, sensitive and accurate techniques are needed and Radioimmunoassay (RIA) has been proposed as an reference method for anabolic agent residues.Faeces, serum and tissue samples of lambs from Ankara and the surrounding countryside were collected and the analyses of zeranol, oestrogen, progesteron and testosteron were carried out using Radioimmunoassay. A total of 153 faeces samples of lambs collected from Ankara and the surrounding countryside were analysed for the presence of zeranol positive samples for lamb faeces were 22%

Keywords

Eostrogen lambs progesterone RIA testosterone zeranol

References

  • Anonim (1973): Yem Kanunu ve Yem Yönetmeliği, Kanun No:1734 Yem Yönetmeliği. Ekler. 3 nolu liste. Karma Yemlere Katılması yasak olan maddeler (A). 13 sayfa 450.
  • Anonim (1988): Yetiştiricilikte kullanılan maddelerin kısa tanımı: 16 Eylül 1988 gün ve EIBD – II. 300. 559.88.1429 sayılı dışişleri Bakanlığı yazısına ilgili,YÖK’ün 27.9.1988 tarih ve 20923 sayılı yazısı ile Ankara Üniversitesi Rektörlüğüne gelen yazı.
  • Blokland MH, Sterk SS, Stephany, RW, Launay FM, Kennedy DG, Van Ginkel, LA (2006): Determination of resorcylic acid lactones in biological samples by GC-MS. Dicrimination between illegal use and contamination with fusarium toxins. Anal Bioanal Chem, 384, 1221-1227.
  • Bloomquist C, Davidson JN, Pearson EG (1982): Zearalenone toxicosis in prepubertal dairy heifer. J Am Vet Med Assoc, 180, 164-165.
  • Commission Decision 93/256/EEC (1993): of April 1993 laying down the methods to be used for detecting residues of substances having a hormonal or thyrostatic action. Off J Europ Com, L118, 64-74.
  • Council Directive 96/22/EC (1996): of 29 April 1996 concerning the prohibition on the use in stockfarming of certain substances having a hormonal or thyrostatic action and beta-agonists, and repealing Directives 81/602/EEC, 88/146/EEC and 88/299.
  • Council Directive 96/23/EC (1996): of 29 April 1996, on measures to monitor certain substances and residues thereof in live animals and animal products. Off J Europ Com, L125, 10-32.
  • Commission Decision 2002/657/EC (2002): Of 12 August 2002 implementing Council Directive 96/23/EC concerning the performance of analytical methods and the interpretation of the results. Off J Europ Com, L221, 8-36.
  • Deschamps JC, Ott RS, Weston PG, Shanks RD, Kesler DJ, Bold DJ, Hixson JE (1987): Effects of zeranol on reproduction in beef bulls: Luteinizing hormone, and testosterone secretion in response to gonadotropin- releasing hormone and human chorionic gonadotropin. Am J Vet Res, 48, 31-36.
  • Dixon SN and Mallinson CB (1986): Radioimmunoassay of the anabolic agent zeranol. III. Zeranol concentration in the faeces of steers implanted with zeranol (Ralgro). J Vet Pharmacol Therap, 9, 88-94.
  • Dixon SN, and Russell KL (1986): Radioimmunoassay of the anabolic agent zeranol. IV. The determination of zeranol concentrations in the edible tissues of cattle implant with zeranol (Ralgro). J Vet Pharmacol Therap, 9, 94-100.
  • Düzgüneş O, Kesici T, Gürbüz F (1983): İstatistik Metodları. Ankara Üniversitesi Ziraat Fakültesi Yayınları No 861, Ankara Üniversitesi Basımevi, Ankara.
  • Egan CL, Wilon LL, Drake TR, Henning WR, Mills EW, Meyer SD, Kenison, DC (1993): Effects of different doses of zeranol on growth, hemoglobin and carcass traits in veal calves, J Anim Sci, 71, 1081-1087.
  • Elsaser TH, Bolt DJ, Bradley BD, Roper M (1983): Acute and chronic changes in adenohypophyseal hormone secertion in sheep during zeranol administration. Am J Vet Res 44, 1068-1071.
  • Farber TM (1991): Anabolics: The approach taken in the USA. Ann Rech Vet, 22, 295-298.
  • Field RA, Snowder GD, Maiorano G, McCormick RJ, Riley ML (1993): Growth and slauther charactiristics of ram and wether lambs implanted with zeranol. J Anim Sci, 71, 631-635.
  • Flowers B, Cantley T and Day BN (1987): A comparison of effects of zearalenone and estradiol benzoate on reproductive function during the estrous cycle in gilt. J Anim Sci, 71, 631-63.
  • Heitzman RJ (1980): Manipulation of protein metabolism with special reference to anabolic agent. 193-203. In: Protein Deposition in Animals. Butterworths London- Boston.
  • Karg H and Vogt K (1981): Residues of DES in veal calves. In: Anabolic Agents in Beef and Veal Production. Proceedings of a workshop held at Brusses, March 5th and 6th.
  • Karg H (1985): Neue Dimensionen der endokrinologie und ihre Bedeutung für die Tierproduction Prak Tierarzt, 66, 103-109.
  • Kindred TP, Hubbert WT (1993): Residue prevention strategies in the United States, JAVMA, 202, 46-49.
  • Kleinova M, Zollner P, Kahlbacher H, Hochsteiner W, Lindner W (2002): Metabolic profiles of the mycotoxin zearalenone and of the growth promoter zeranol in urine, liver, and muscle of heifers. J Agric Food Chem, 50, 4769- 4776.
  • Lage IG, Daxenberger A, Meyer, HH (2001): Hormone concents in peripheral tissues after correct and off-label use of growth promoting hormones in cattle: effect of the implant preparations Filaplix-H, Raglo, Synovex-H and Synovex Plus. APMIS, 109, 53-65.
  • Lange IG, Daxenberger A, Meyer HHD, Rajperd-De Meyts e, Skakkeb NE, and Veeramachaneni DNR (2002): Quantitative assesment of foetal exposure to trenbolone acetate, zeranol and melengestrol acetate, following maternal dosing in rabbits. Xenobiotica, 32, 641-651.
  • Launay FM, Young PB, Sters SS, Blokland MH, Kennedy DG (2004): Confirmatory assay for zeranol, taleranol and the Fusarium spp. Toxins in bovine urine using liquid chromatography-tandem mass spectrometry. Food Add Cont, 21, 52-62.
  • Metzler M (1989): Metabolism of some anabolic agent: Toxicological and analytical aspects. J Chromatography, 489, 11-21.
  • Moran C, Quirke JF, Prendiville DJ, Bourke S, Roche JF (1991): The effect of estradiol, trenbolone acetate, or zeranol on growth rate, mammary developlent, carcass traits and plasma estradiol concentrations of beef heifers. J Anim Sci 69, 4249-4258.
  • Southgate JR, Peters AR, Dixon SN (1988): Effects of oestradiol-17 Beta or zeranol with or without trenbolone acetate on live-weight gain, carcass composition and zeranol residues in stress on an 18-Month Beef System. Anim Produc, 47, 209-214.
  • Sundlof SF, Stricland C. (1986): Zeralone and zeranol: Potential residues problems in livestock. Vet Hum Toxicol 28, 242-250.
  • Taguchi S, Yoshida S, Tanaka Y, Hori S (2001): Simple and rapid analysis of trenbolone and zeranol residues in cattle muscle and liver by stack-cartridge solid-phase extraction and HPLC using on-line clean-up with EC and UV detection. Shokuhin Eiseigaku Zasshi, 42, 226-230. Geliş tarihi: 02.07.2006 / Kabul tarihi : 06.07.2006 Address for correspondance: Prof. Dr. Hilal Karagül Ankara University, Faculty of Veterinary Medicine Department of Biochemistry 06110 Dışkapı-Ankara

Ankara ve çevresinde kuzularda zeranol artık düzeyleri ile serum testesteron, östrojen ve progesterone düzeyleri

Year 2007, Volume: 54 Issue: 1, 7 - 10, 01.03.2007

Güzin Özkurt Borazan Hilal Karagül Sefa Çelik Nil Ünal Mert Pekcan Tevhide Sel

https://doi.org/10.1501/Vetfak_0000000247
Cited By: 1

Abstract

Zearalenone’un sentetik bir derivesi olan Zeranol, koyun ve sığırlarda besi performansını artırmak amacıyla anabolik madde olarak kullanılmıştır. Avrupa Birliği ülkelerinde ve Türkiye’de tüketiciye ulaşabilecek kalıntıları nedeniyle hayvanlarda kullanımı yasaklanmıştır. Yasal olmayan kullanımlarında, verilen miktar ve süre bilinmediğinden tesbiti güçtür. Bunun yanında hayvanın yası, cinsi ve bireysel farklılıklar da zeranol’ün vücuttan uzaklaşmasında etkilidir. Bu nedenle hızlı, duyarlı teknikler kullanılmalıdır ve Radioimmunoassay, referans metot olarak önerilmektedir. Ankara çevresinde çiftlik ve mezbahalardan toplanan dışkı ve doku örnelerinde zeranol analizleri ile serum örneklerinde testosteron, progesteron ve östrojen hormon analizleri Radioimmunoassay ile yapılmıştır. Toplam 153 kuzu dışkı örneği zeranol yönünden analiz edildi. Kuzularda % 22 zeranol pozitif bulundu

Keywords

Kuzu östrojen progesteron RIA testosteron zeranol

References

  • Anonim (1973): Yem Kanunu ve Yem Yönetmeliği, Kanun No:1734 Yem Yönetmeliği. Ekler. 3 nolu liste. Karma Yemlere Katılması yasak olan maddeler (A). 13 sayfa 450.
  • Anonim (1988): Yetiştiricilikte kullanılan maddelerin kısa tanımı: 16 Eylül 1988 gün ve EIBD – II. 300. 559.88.1429 sayılı dışişleri Bakanlığı yazısına ilgili,YÖK’ün 27.9.1988 tarih ve 20923 sayılı yazısı ile Ankara Üniversitesi Rektörlüğüne gelen yazı.
  • Blokland MH, Sterk SS, Stephany, RW, Launay FM, Kennedy DG, Van Ginkel, LA (2006): Determination of resorcylic acid lactones in biological samples by GC-MS. Dicrimination between illegal use and contamination with fusarium toxins. Anal Bioanal Chem, 384, 1221-1227.
  • Bloomquist C, Davidson JN, Pearson EG (1982): Zearalenone toxicosis in prepubertal dairy heifer. J Am Vet Med Assoc, 180, 164-165.
  • Commission Decision 93/256/EEC (1993): of April 1993 laying down the methods to be used for detecting residues of substances having a hormonal or thyrostatic action. Off J Europ Com, L118, 64-74.
  • Council Directive 96/22/EC (1996): of 29 April 1996 concerning the prohibition on the use in stockfarming of certain substances having a hormonal or thyrostatic action and beta-agonists, and repealing Directives 81/602/EEC, 88/146/EEC and 88/299.
  • Council Directive 96/23/EC (1996): of 29 April 1996, on measures to monitor certain substances and residues thereof in live animals and animal products. Off J Europ Com, L125, 10-32.
  • Commission Decision 2002/657/EC (2002): Of 12 August 2002 implementing Council Directive 96/23/EC concerning the performance of analytical methods and the interpretation of the results. Off J Europ Com, L221, 8-36.
  • Deschamps JC, Ott RS, Weston PG, Shanks RD, Kesler DJ, Bold DJ, Hixson JE (1987): Effects of zeranol on reproduction in beef bulls: Luteinizing hormone, and testosterone secretion in response to gonadotropin- releasing hormone and human chorionic gonadotropin. Am J Vet Res, 48, 31-36.
  • Dixon SN and Mallinson CB (1986): Radioimmunoassay of the anabolic agent zeranol. III. Zeranol concentration in the faeces of steers implanted with zeranol (Ralgro). J Vet Pharmacol Therap, 9, 88-94.
  • Dixon SN, and Russell KL (1986): Radioimmunoassay of the anabolic agent zeranol. IV. The determination of zeranol concentrations in the edible tissues of cattle implant with zeranol (Ralgro). J Vet Pharmacol Therap, 9, 94-100.
  • Düzgüneş O, Kesici T, Gürbüz F (1983): İstatistik Metodları. Ankara Üniversitesi Ziraat Fakültesi Yayınları No 861, Ankara Üniversitesi Basımevi, Ankara.
  • Egan CL, Wilon LL, Drake TR, Henning WR, Mills EW, Meyer SD, Kenison, DC (1993): Effects of different doses of zeranol on growth, hemoglobin and carcass traits in veal calves, J Anim Sci, 71, 1081-1087.
  • Elsaser TH, Bolt DJ, Bradley BD, Roper M (1983): Acute and chronic changes in adenohypophyseal hormone secertion in sheep during zeranol administration. Am J Vet Res 44, 1068-1071.
  • Farber TM (1991): Anabolics: The approach taken in the USA. Ann Rech Vet, 22, 295-298.
  • Field RA, Snowder GD, Maiorano G, McCormick RJ, Riley ML (1993): Growth and slauther charactiristics of ram and wether lambs implanted with zeranol. J Anim Sci, 71, 631-635.
  • Flowers B, Cantley T and Day BN (1987): A comparison of effects of zearalenone and estradiol benzoate on reproductive function during the estrous cycle in gilt. J Anim Sci, 71, 631-63.
  • Heitzman RJ (1980): Manipulation of protein metabolism with special reference to anabolic agent. 193-203. In: Protein Deposition in Animals. Butterworths London- Boston.
  • Karg H and Vogt K (1981): Residues of DES in veal calves. In: Anabolic Agents in Beef and Veal Production. Proceedings of a workshop held at Brusses, March 5th and 6th.
  • Karg H (1985): Neue Dimensionen der endokrinologie und ihre Bedeutung für die Tierproduction Prak Tierarzt, 66, 103-109.
  • Kindred TP, Hubbert WT (1993): Residue prevention strategies in the United States, JAVMA, 202, 46-49.
  • Kleinova M, Zollner P, Kahlbacher H, Hochsteiner W, Lindner W (2002): Metabolic profiles of the mycotoxin zearalenone and of the growth promoter zeranol in urine, liver, and muscle of heifers. J Agric Food Chem, 50, 4769- 4776.
  • Lage IG, Daxenberger A, Meyer, HH (2001): Hormone concents in peripheral tissues after correct and off-label use of growth promoting hormones in cattle: effect of the implant preparations Filaplix-H, Raglo, Synovex-H and Synovex Plus. APMIS, 109, 53-65.
  • Lange IG, Daxenberger A, Meyer HHD, Rajperd-De Meyts e, Skakkeb NE, and Veeramachaneni DNR (2002): Quantitative assesment of foetal exposure to trenbolone acetate, zeranol and melengestrol acetate, following maternal dosing in rabbits. Xenobiotica, 32, 641-651.
  • Launay FM, Young PB, Sters SS, Blokland MH, Kennedy DG (2004): Confirmatory assay for zeranol, taleranol and the Fusarium spp. Toxins in bovine urine using liquid chromatography-tandem mass spectrometry. Food Add Cont, 21, 52-62.
  • Metzler M (1989): Metabolism of some anabolic agent: Toxicological and analytical aspects. J Chromatography, 489, 11-21.
  • Moran C, Quirke JF, Prendiville DJ, Bourke S, Roche JF (1991): The effect of estradiol, trenbolone acetate, or zeranol on growth rate, mammary developlent, carcass traits and plasma estradiol concentrations of beef heifers. J Anim Sci 69, 4249-4258.
  • Southgate JR, Peters AR, Dixon SN (1988): Effects of oestradiol-17 Beta or zeranol with or without trenbolone acetate on live-weight gain, carcass composition and zeranol residues in stress on an 18-Month Beef System. Anim Produc, 47, 209-214.
  • Sundlof SF, Stricland C. (1986): Zeralone and zeranol: Potential residues problems in livestock. Vet Hum Toxicol 28, 242-250.
  • Taguchi S, Yoshida S, Tanaka Y, Hori S (2001): Simple and rapid analysis of trenbolone and zeranol residues in cattle muscle and liver by stack-cartridge solid-phase extraction and HPLC using on-line clean-up with EC and UV detection. Shokuhin Eiseigaku Zasshi, 42, 226-230. Geliş tarihi: 02.07.2006 / Kabul tarihi : 06.07.2006 Address for correspondance: Prof. Dr. Hilal Karagül Ankara University, Faculty of Veterinary Medicine Department of Biochemistry 06110 Dışkapı-Ankara
There are 30 citations in total.

Details

Primary Language English
Subjects Veterinary Surgery
Journal Section Research Article
Authors

Güzin Özkurt Borazan

Hilal Karagül

Sefa Çelik

Nil Ünal

Mert Pekcan

Tevhide Sel

Publication Date March 1, 2007
Published in Issue Year 2007Volume: 54 Issue: 1

Cite

APA Borazan, G. Ö., Karagül, H., Çelik, S., Ünal, N., et al. (2007). Determination of zeranol residues and the serum testosterone oestrogene and progesterone levels in lambs around Ankara region. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 54(1), 7-10. https://doi.org/10.1501/Vetfak_0000000247
AMA Borazan GÖ, Karagül H, Çelik S, Ünal N, Pekcan M, Sel T. Determination of zeranol residues and the serum testosterone oestrogene and progesterone levels in lambs around Ankara region. Ankara Univ Vet Fak Derg. March 2007;54(1):7-10. doi:10.1501/Vetfak_0000000247
Chicago Borazan, Güzin Özkurt, Hilal Karagül, Sefa Çelik, Nil Ünal, Mert Pekcan, and Tevhide Sel. “Determination of Zeranol Residues and the Serum Testosterone Oestrogene and Progesterone Levels in Lambs Around Ankara Region”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 54, no. 1 (March 2007): 7-10. https://doi.org/10.1501/Vetfak_0000000247.
EndNote Borazan GÖ, Karagül H, Çelik S, Ünal N, Pekcan M, Sel T (March 1, 2007) Determination of zeranol residues and the serum testosterone oestrogene and progesterone levels in lambs around Ankara region. Ankara Üniversitesi Veteriner Fakültesi Dergisi 54 1 7–10.
IEEE G. Ö. Borazan, H. Karagül, S. Çelik, N. Ünal, M. Pekcan, and T. Sel, “Determination of zeranol residues and the serum testosterone oestrogene and progesterone levels in lambs around Ankara region”, Ankara Univ Vet Fak Derg, vol. 54, no. 1, pp. 7–10, 2007, doi: 10.1501/Vetfak_0000000247.
ISNAD Borazan, Güzin Özkurt et al. “Determination of Zeranol Residues and the Serum Testosterone Oestrogene and Progesterone Levels in Lambs Around Ankara Region”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 54/1 (March 2007), 7-10. https://doi.org/10.1501/Vetfak_0000000247.
JAMA Borazan GÖ, Karagül H, Çelik S, Ünal N, Pekcan M, Sel T. Determination of zeranol residues and the serum testosterone oestrogene and progesterone levels in lambs around Ankara region. Ankara Univ Vet Fak Derg. 2007;54:7–10.
MLA Borazan, Güzin Özkurt et al. “Determination of Zeranol Residues and the Serum Testosterone Oestrogene and Progesterone Levels in Lambs Around Ankara Region”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 54, no. 1, 2007, pp. 7-10, doi:10.1501/Vetfak_0000000247.
Vancouver Borazan GÖ, Karagül H, Çelik S, Ünal N, Pekcan M, Sel T. Determination of zeranol residues and the serum testosterone oestrogene and progesterone levels in lambs around Ankara region. Ankara Univ Vet Fak Derg. 2007;54(1):7-10.

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