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Detection of subclinical mastitis by clinical, microbiological and biochemical methods in sheep

Year 2006, Volume: 53 Issue: 1, 31 - 35, 01.03.2006

İlknur Pir Yağcı Mustafa Kaymaz

https://doi.org/10.1501/Vetfak_0000000060

Abstract

The aim of this study was to determine the incidence and aetiology of subclinical ovine mastitis in Kırıkkale and to study the changes occurring in the level of chlorine, sodium, ALP, LDH and NA-Gase, SCC and California Mastitis Test (CMT) scores in milk as a result of subclinical mastitis in 200 sheep. Coagulase negative staphylococci were isolated in 5.5% of the milk samples. The period insidence of subclinical mastitis was detected as 6,5%. Milk samples from noninfected udder halves were found to have variable CMT scores. In milk samples bacteriologically positive , SCC was measured as 300.000-1.000.000 and in milk samples bacteriologically negative ≤300.000. In bacteriologically negative milk samples, the sodium and clorine levels were measured as 56.26 and 47.13 mg/dl respectively while they were found to be 78.65 and 72.67 mg/dl in the bacteriological positive milk samples. In milk from healthy udders, ALP, LDH and NA-Gase enzymes activities were measured as 110.12, 65.60 and 23.51 U/L respectively while they were found to be 194.69, 452.23 and 79.38 U/L in milk from subclinical mastitic udders. The difference between the samples from normal and subclinical mastitis for SCC and biochemical parameters were found to be statistically significant at the level of p

Keywords

Biochemical profiles CMT ewe SCC subclinical mastitis

References

  • 1. Alaçam E (1997): Meme Hastalıkları. (In): E. Alaçam ve M. Şahal, Sığır Hastalıkları. Medisan, Ankara. 389-425.
  • 2. Alais C (1974): Science du Lait.(In): Principles des Techiniques Laiteres. Editeus Boulevard, Paris.
  • 3. Albenzio M, Taibi L, Muscio A, Sevi A (2002): Prevalence and etiology of subclinical mastitis in intensively managed flocs and related changes in the yield and quality of ewe milk. Small Rum Res, 43, 219-226.
  • 4. Batavani RA, Mortaz E, Falahian K, Dawoodi MA (2003): Study on frequency, a etiology and some enzymatic activities of subclinical ovine mastitis in Urmia, Iran. Small Rum Res, 50, 45-50.
  • 5. Batu A, Fırat G (1981): Trakya ve Marmara Bölgesinde koyunlarda klinik ve subklinik mastitis ve etkenleri üzerine araştırma. Pendik Vet Mikrob Araş Ens Derg, 13, 11-21.
  • 6. Baysal T, Kenar B (1989): Konya ve yöresindeki koyunlarda klinik ve subklinik mastitis olgularından aerob etken izolasyon ve identifikasyonu. Etlik Vet Mikrob Derg, 6, 55-56.
  • 7. Bergonier D, Berthelot X (2003): New advances in epizootiology and control of ewe mastitis. Livestock Prod Sci, 79, 1-16.
  • 8. Bergonier D, De Cremoux R, Rupp R, Lagriffoul G, Berthelot X (2003): Mastitis of dairy small ruminats. Vet Res, 34, 689-716.
  • 9. Billon P, Decremoux R (1998): Mastitis of dairy ewes: etiology, detection and control. 4th Great Lakes Dairy Sheep Symposium, 25-27 June 1998, Spooner, Wisconsin, USA.
  • 10. Bogin E, Ziv G (1973): Enzymes and minerals in normal and mastitic milk. Cornell Vet, 63, 666-676.
  • 11. Dela Cruz M, Serrano E, Monotoro U, Marco J, Romeo M, Baselga R, Albizu I, Amorena B (1994): Etiology and prevalence of subclinical mastitis in the Manchega sheep at mid-late lactation. Erişim: [http://www.elsevier.nl/gejng/abstract.html]. Erişim Tarihi: 24.04.2001.
  • 12. Ergun H, Mert N (1984): Sütte mastitis nedeniyle meydana gelen biyokimyasal değişmeler. I. Mastitis Semineri, 24 Kasım 1984, Ankara Üniversitesi Veteriner Fakültesi, Ankara, 49-61
  • 13. Fthenakis GC, El-Massanat ETS, Booth JM, Jones JET (1991): Somatic cell counts of ewes milk. Br Vet J, 147, 575-581.
  • 14. Gonzalez-Rodriguez MC, Gonzalo C, San Primitivo F, Carmenes P (1995): Relationship between somatic cell count and intramammary infection of the half udder in dairy ewes. J Dairy Sci, 78, 2753-2759.
  • 15. Green TJ (1984): Use of somatic cell counts for detection of subclinical mastitis in ewes. Vet Rec, 114, 43-49.
  • 16. Henry RJ (1974): Clinical Chemistry Principles and Technics, 2nd Ed., Harper Collins, Hagerstown.
  • 17. Houeston VD, Hartwig NR, Judy JK (1986): Detection of ovine intramammary infection with California mastitis test. JAVMA, 188, 522-524.
  • 18. Internatıonal Dairy Federation (1981): Laboratory methods for use in mastitis work, Document: 132, Brussels.
  • 19. Kılıçoğlu Ç, Alaçam E, İzgür H, Akay Ö, Wiesner HU (1989): Eutergesundheitskontrolle von Milchkülen im Gebiet von Ankara (Turkei). Dtsch. Tierärztl Wschr, 96, 486-488.
  • 20. Kitchen BJ, Middleton G, Salmon M (1978): Bovine milk N-acetyl beta-D- glucosaminidase and its significance in detection of abnormal udder secretion. J Dairy Sci, 45, 15-20.
  • 21. Las Heras A, Dominguez L, Fernandez-Garayzabal JF (1999): Prevalence and aetiology of subclinical mastitis in dairy ewes of the Madrid region. Erişim: [http://www.elsevier.nl/gej-ng/abstract.html]. Erişim Tarihi: 20.04.2001.
  • 22. Leitner G, Chaffer M, Caraso Y, Ezra E, Kababea D, Winkler M, Glickman A, Saran A (2003): Udder infection and milk somatic cell count, NA-Gase activity and milk composition-fat, protein and lactose in IsraeliAssaf and Awassi sheep. Small Rum Res, 49, 157-164.
  • 23. Leitner G, Chaffer M, Shamay A, Shapiro F, Merin U, Ezra E, Saran, A, Silanikove N (2004): Changes in milk composition as affected by subclinical mastitis in sheep. J Dairy Sci, 87, 46-52.
  • 24. Maisi P, Junttialla J, Seppanen J (1987): Detection of subclinical mastitis in ewes. Br Vet J, 143, 402-409.
  • 25. Mavrogenis AP, Koumas A, Kakoyianis CK, Taliotis CH (1995): Use of somatic cell counts for detection of subclinical mastitis in sheep. Erişim: [http://www.elsevier.nl/gej-ng/abstract.html]. Erişim Tarihi: 25.04.2001.
  • 26. McDougall S, Pankey W, Delaney C, Barlow J, Murdough PA, Scruton D (2002): Prevalence and incidence of subclinical mastitis in goats and dairy ewes in Vermont, USA. Small Rum Res, 46, 115-121.
  • 27. Nizamlıoğlu M, Erganiş O (1991): Suitability of laktate dehydrogenase activity and somatic cell counts of milk for detection of subclinical mastitis in Merino ewes. Acta Vet Hung, 39, 21-23.
  • 28. Nizamlıoğlu M, Tekeli T, Erganiş O, Başpınar N (1989): İneklerde subklinik mastitislerin biyokimyasal ve mikrobiyolojik yönden incelenmesi. Selçuk Üniv Vet Fak Derg, 5, 135-143.
  • 29. Nizamlıoğlu M, Dinç DA, Erganiş O, Özeren F, Uçan S (1992): Subklinik mastitisli koyunlarda N-asetil β-D glukozaminidaz enzimi ve bazı biyokimyasal değerlerin araştırılması. Veterinarium, 3, 12-16.
  • 30. Özdamar K (2003): SPSS ile Biyoistatistik, Kaan Kitabevi, Eskişehir.
  • 31. Regi G, Honegger R, Buchi S, Segessemann V, Rusch P (1991): Cell count and Schalm test results of milk from dairy sheep with healthy udders during the course of a complete lactation. Erişim: [http://www.ncbi.nlm. nih.gov:80/entrez/que…db=Pubmed& list_uids=2047833 &dopt=Abstract]. Erişim tarihi 18.04.2001.
  • 32. Schalm OW, Carroll EJ, Jain NC (1971): Bovine Mastitis. Lea-Febiger, Philadelphia.

Koyunlarda klinik, mikrobiyolojik ve biyokimyasal metotlar ile subklinik mastitislerin saptanması

Year 2006, Volume: 53 Issue: 1, 31 - 35, 01.03.2006

İlknur Pir Yağcı Mustafa Kaymaz

https://doi.org/10.1501/Vetfak_0000000060

Abstract

Bu çalışmanın amacı, koyunlarda subklinik mastitisin insidansını, etiyolojisini ve subklinik mastitis sonucu sütteki klor (Cl-), sodyum (Na+), ALP, LDH ve NA-Gaz düzeyindeki, SHS ve CMT skorlarındaki değişimleri tespit etmektir. Örneklerin %5.5’inde koagulaz negatif stafilokoklar izole edildi. Subklinik mastitisin prevalansı %6.5 seviyesinde belirlendi. Normal örneklerdeki CMT sonuçları değişken bulundu. Somatik hücre sayısı subklinik mastitisli örneklerde 300.000-1.000.000 ve normal örneklerde ≤300.000 saptandı. Subklinik mastitisli örneklerde Na+ ve Cl- düzeyleri sırasıyla 56.26 ve 47.13 mg/dl, normal örneklerde ise 78.65 ve 72.67 mg/dl bulundu. Normal süt örneklerinde ALP, LDH ve NA-Gaz düzeyleri sırasıyla 110.12, 65.60 ve 23.51 U/L, subklinik mastitisli örneklerde sırasıyla 194.69, 452.23 ve 79.38 U/L tespit edildi. Normal ve subklinik mastitisli örneklerin SHS ve biyokimyasal sonuçlarının istatistiki değerlendirmesinde iki grup arasındaki fark p

Keywords

Biyokimyasal profil CMT koyun SHS subklinik mastitis

References

  • 1. Alaçam E (1997): Meme Hastalıkları. (In): E. Alaçam ve M. Şahal, Sığır Hastalıkları. Medisan, Ankara. 389-425.
  • 2. Alais C (1974): Science du Lait.(In): Principles des Techiniques Laiteres. Editeus Boulevard, Paris.
  • 3. Albenzio M, Taibi L, Muscio A, Sevi A (2002): Prevalence and etiology of subclinical mastitis in intensively managed flocs and related changes in the yield and quality of ewe milk. Small Rum Res, 43, 219-226.
  • 4. Batavani RA, Mortaz E, Falahian K, Dawoodi MA (2003): Study on frequency, a etiology and some enzymatic activities of subclinical ovine mastitis in Urmia, Iran. Small Rum Res, 50, 45-50.
  • 5. Batu A, Fırat G (1981): Trakya ve Marmara Bölgesinde koyunlarda klinik ve subklinik mastitis ve etkenleri üzerine araştırma. Pendik Vet Mikrob Araş Ens Derg, 13, 11-21.
  • 6. Baysal T, Kenar B (1989): Konya ve yöresindeki koyunlarda klinik ve subklinik mastitis olgularından aerob etken izolasyon ve identifikasyonu. Etlik Vet Mikrob Derg, 6, 55-56.
  • 7. Bergonier D, Berthelot X (2003): New advances in epizootiology and control of ewe mastitis. Livestock Prod Sci, 79, 1-16.
  • 8. Bergonier D, De Cremoux R, Rupp R, Lagriffoul G, Berthelot X (2003): Mastitis of dairy small ruminats. Vet Res, 34, 689-716.
  • 9. Billon P, Decremoux R (1998): Mastitis of dairy ewes: etiology, detection and control. 4th Great Lakes Dairy Sheep Symposium, 25-27 June 1998, Spooner, Wisconsin, USA.
  • 10. Bogin E, Ziv G (1973): Enzymes and minerals in normal and mastitic milk. Cornell Vet, 63, 666-676.
  • 11. Dela Cruz M, Serrano E, Monotoro U, Marco J, Romeo M, Baselga R, Albizu I, Amorena B (1994): Etiology and prevalence of subclinical mastitis in the Manchega sheep at mid-late lactation. Erişim: [http://www.elsevier.nl/gejng/abstract.html]. Erişim Tarihi: 24.04.2001.
  • 12. Ergun H, Mert N (1984): Sütte mastitis nedeniyle meydana gelen biyokimyasal değişmeler. I. Mastitis Semineri, 24 Kasım 1984, Ankara Üniversitesi Veteriner Fakültesi, Ankara, 49-61
  • 13. Fthenakis GC, El-Massanat ETS, Booth JM, Jones JET (1991): Somatic cell counts of ewes milk. Br Vet J, 147, 575-581.
  • 14. Gonzalez-Rodriguez MC, Gonzalo C, San Primitivo F, Carmenes P (1995): Relationship between somatic cell count and intramammary infection of the half udder in dairy ewes. J Dairy Sci, 78, 2753-2759.
  • 15. Green TJ (1984): Use of somatic cell counts for detection of subclinical mastitis in ewes. Vet Rec, 114, 43-49.
  • 16. Henry RJ (1974): Clinical Chemistry Principles and Technics, 2nd Ed., Harper Collins, Hagerstown.
  • 17. Houeston VD, Hartwig NR, Judy JK (1986): Detection of ovine intramammary infection with California mastitis test. JAVMA, 188, 522-524.
  • 18. Internatıonal Dairy Federation (1981): Laboratory methods for use in mastitis work, Document: 132, Brussels.
  • 19. Kılıçoğlu Ç, Alaçam E, İzgür H, Akay Ö, Wiesner HU (1989): Eutergesundheitskontrolle von Milchkülen im Gebiet von Ankara (Turkei). Dtsch. Tierärztl Wschr, 96, 486-488.
  • 20. Kitchen BJ, Middleton G, Salmon M (1978): Bovine milk N-acetyl beta-D- glucosaminidase and its significance in detection of abnormal udder secretion. J Dairy Sci, 45, 15-20.
  • 21. Las Heras A, Dominguez L, Fernandez-Garayzabal JF (1999): Prevalence and aetiology of subclinical mastitis in dairy ewes of the Madrid region. Erişim: [http://www.elsevier.nl/gej-ng/abstract.html]. Erişim Tarihi: 20.04.2001.
  • 22. Leitner G, Chaffer M, Caraso Y, Ezra E, Kababea D, Winkler M, Glickman A, Saran A (2003): Udder infection and milk somatic cell count, NA-Gase activity and milk composition-fat, protein and lactose in IsraeliAssaf and Awassi sheep. Small Rum Res, 49, 157-164.
  • 23. Leitner G, Chaffer M, Shamay A, Shapiro F, Merin U, Ezra E, Saran, A, Silanikove N (2004): Changes in milk composition as affected by subclinical mastitis in sheep. J Dairy Sci, 87, 46-52.
  • 24. Maisi P, Junttialla J, Seppanen J (1987): Detection of subclinical mastitis in ewes. Br Vet J, 143, 402-409.
  • 25. Mavrogenis AP, Koumas A, Kakoyianis CK, Taliotis CH (1995): Use of somatic cell counts for detection of subclinical mastitis in sheep. Erişim: [http://www.elsevier.nl/gej-ng/abstract.html]. Erişim Tarihi: 25.04.2001.
  • 26. McDougall S, Pankey W, Delaney C, Barlow J, Murdough PA, Scruton D (2002): Prevalence and incidence of subclinical mastitis in goats and dairy ewes in Vermont, USA. Small Rum Res, 46, 115-121.
  • 27. Nizamlıoğlu M, Erganiş O (1991): Suitability of laktate dehydrogenase activity and somatic cell counts of milk for detection of subclinical mastitis in Merino ewes. Acta Vet Hung, 39, 21-23.
  • 28. Nizamlıoğlu M, Tekeli T, Erganiş O, Başpınar N (1989): İneklerde subklinik mastitislerin biyokimyasal ve mikrobiyolojik yönden incelenmesi. Selçuk Üniv Vet Fak Derg, 5, 135-143.
  • 29. Nizamlıoğlu M, Dinç DA, Erganiş O, Özeren F, Uçan S (1992): Subklinik mastitisli koyunlarda N-asetil β-D glukozaminidaz enzimi ve bazı biyokimyasal değerlerin araştırılması. Veterinarium, 3, 12-16.
  • 30. Özdamar K (2003): SPSS ile Biyoistatistik, Kaan Kitabevi, Eskişehir.
  • 31. Regi G, Honegger R, Buchi S, Segessemann V, Rusch P (1991): Cell count and Schalm test results of milk from dairy sheep with healthy udders during the course of a complete lactation. Erişim: [http://www.ncbi.nlm. nih.gov:80/entrez/que…db=Pubmed& list_uids=2047833 &dopt=Abstract]. Erişim tarihi 18.04.2001.
  • 32. Schalm OW, Carroll EJ, Jain NC (1971): Bovine Mastitis. Lea-Febiger, Philadelphia.
There are 32 citations in total.

Details

Primary Language Turkish
Subjects Veterinary Surgery
Journal Section Research Article
Authors

İlknur Pir Yağcı

Mustafa Kaymaz

Publication Date March 1, 2006
Published in Issue Year 2006Volume: 53 Issue: 1

Cite

APA Pir Yağcı, İ., & Kaymaz, M. (2006). Koyunlarda klinik, mikrobiyolojik ve biyokimyasal metotlar ile subklinik mastitislerin saptanması. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 53(1), 31-35. https://doi.org/10.1501/Vetfak_0000000060
AMA Pir Yağcı İ, Kaymaz M. Koyunlarda klinik, mikrobiyolojik ve biyokimyasal metotlar ile subklinik mastitislerin saptanması. Ankara Univ Vet Fak Derg. March 2006;53(1):31-35. doi:10.1501/Vetfak_0000000060
Chicago Pir Yağcı, İlknur, and Mustafa Kaymaz. “Koyunlarda Klinik, Mikrobiyolojik Ve Biyokimyasal Metotlar Ile Subklinik Mastitislerin Saptanması”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 53, no. 1 (March 2006): 31-35. https://doi.org/10.1501/Vetfak_0000000060.
EndNote Pir Yağcı İ, Kaymaz M (March 1, 2006) Koyunlarda klinik, mikrobiyolojik ve biyokimyasal metotlar ile subklinik mastitislerin saptanması. Ankara Üniversitesi Veteriner Fakültesi Dergisi 53 1 31–35.
IEEE İ. Pir Yağcı and M. Kaymaz, “Koyunlarda klinik, mikrobiyolojik ve biyokimyasal metotlar ile subklinik mastitislerin saptanması”, Ankara Univ Vet Fak Derg, vol. 53, no. 1, pp. 31–35, 2006, doi: 10.1501/Vetfak_0000000060.
ISNAD Pir Yağcı, İlknur - Kaymaz, Mustafa. “Koyunlarda Klinik, Mikrobiyolojik Ve Biyokimyasal Metotlar Ile Subklinik Mastitislerin Saptanması”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 53/1 (March 2006), 31-35. https://doi.org/10.1501/Vetfak_0000000060.
JAMA Pir Yağcı İ, Kaymaz M. Koyunlarda klinik, mikrobiyolojik ve biyokimyasal metotlar ile subklinik mastitislerin saptanması. Ankara Univ Vet Fak Derg. 2006;53:31–35.
MLA Pir Yağcı, İlknur and Mustafa Kaymaz. “Koyunlarda Klinik, Mikrobiyolojik Ve Biyokimyasal Metotlar Ile Subklinik Mastitislerin Saptanması”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 53, no. 1, 2006, pp. 31-35, doi:10.1501/Vetfak_0000000060.
Vancouver Pir Yağcı İ, Kaymaz M. Koyunlarda klinik, mikrobiyolojik ve biyokimyasal metotlar ile subklinik mastitislerin saptanması. Ankara Univ Vet Fak Derg. 2006;53(1):31-5.