Letter to Editor
Characterization of Staphylococcus aureus strains isolated from subclinic bovine mastitis by protein patterns, antibiotic resistance and plasmid profile
Abstract
A total of 50 Staphylococcus aureus strains isolated from bovine subclinical mastitis in Hatay region were
characterized by protein patterns, antibiotic resistance and plasmid profiles. SDS-PAGE analysis of whole-cell protein extracts
belong to S. aureus strains produced patterns containing 17-49 discrete bands with molecular weights of >14.4-<116 kDa. S. aureus
strains clustered into two cluster on the basis of a computer-assisted numerical analysis. The resistance rates of 50 of S. aureus strains
to trimethoprim-sulphamethoxazole, danofloxacin, amoxicillin-clavulanic, sulbactam-ampicillin, erythromycin, lincomycin,
cefoperazone, oxytetracycline, amoxicillin, streptomycin and penicillin were 4 %, 4 %, 10 %, 14 %, 30 %, 34 %, 36 %, 38 %, 50 %,
54 % and 68 %, respectively. Plasmid profiling demonstrated that 47 of the 50 S. aureus strains contained plasmid ranging from 1.7
to >24 kb.
References
- 1. Aarestrop FM, Wegener HC, Rosdahl VT (1995): Evaluation of phenotypic and genotypic methods for epidemiological typing of Staphylococcus aureus isolates from bovine mastitis in Denmark. Vet Microbiol, 45, 139- 150.
- 2. Anderson DG, McKay LL (1983): Simple and rapid method for isolating large plasmid DNA from lactic Streptococci. Appl Environ Microbiol, 46, 549-552.
- 3. Bannerman TL, Hancock GA, Tenover FC, Miller JM (1995): Pulsed-field gel electrophoresis as a replacement for bacteriphage typing of Staphylococcus aureus. J Clin Microbiol, 33, 551-555.
- 4. Bauer AW, Kirby WM, Sherris JC, Turck M (1966): Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol, 45, 493-496.
- 5. Baumgartner A, Nicolet J, Eggimann M (1984): Plasmid profiles of Staphylococcus aureus causing bovine mastitis. J Appl Bacteriol, 56, 159-163.
- 6. Bushuk W, Hay RL, Larsen NG, Sara RG, Simmons LD, Sutton KH (1997): Effect of Mechanical Dough Development on the Extractability of Wheat Storage Proteins from Bread Dough. Cereal Chem, 74, 389-395.
- 7. Costas M, Holmes B, Sloss LL (1987): Numerical analysis of electrophoretic protein patterns of Providencia rustigianii strains from human diarrhoea and other sources. J Appl Bacteriol, 63, 319-328.
- 8. Costas M, Cookson BD, Talsania HG, Owen RJ (1989): Numerical analysis of electrophoretic protein patterns of methicillin-resistant strains of Staphylococcus aureus. J Clin Microbiol, 27, 2574-2581.
- 9. Demiralp H, Çelik S, Köksel H (2000): Effects of oxidizing agents and defatting on the electrophoretic patterns of flour proteins during dough mixing. Eur Food Res Technol, 211, 322-325.
- 10. Fitzgerald JR, Meaney WJ, Hartigan PJ, Smyth CJ, Kapur V (1997): Fine-structure molecular epidemiological analysis of Staphylococcus aureus recovered from cows. Epidemiol Infect, 119, 261-269.
- 11. Goh SH, Byrne SK, Zhang JL, Chow AW (1992): Molecular typing of Staphylococcus aureus on the basis of coagulase gene polymorphisms. J Clin Microbiol, 30, 1642-1645.
- 12. Kapur V, Sischo WM, Greer RS, Whittam TS, Musser JM (1995): Molecular population genetic analysis of Staphylococcus aureus recovered from cows. J Clin Microbiol, 33, 376-380.
- 13. Kishore L, Natarajan K, Babu LR (1996): Total soluble protein and membrane lipopolysaccharide profiles in differentiating Rhizobium isolates. Microbios, 86, 143-156.
- 14. Koneman EM, Allen SD, Dovell VR, Sommers HM, Winn WC (1997): Color Atlas and Textbook of Diagnostic Microbiology. JP Lippincott Comp, Philadelphia
- 15. Laemmli UK (1970): Cleavege of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-684.
- 16. Lange C, Cardoso M, Senczek D, Schwarz S (1999): Molecular subtyping of Staphylococcus aureus isolates from cases of bovine mastitis in Brazil. Vet Microbiol, 67, 127-141.
- 17. Lopes CA, Moreno G, Curi PR, Gottschalk AF, Modolo JR, Horacio A, Correa A, Pavan C (1990): Charecteristics of Staphylococcus aureus from subclinical bovine mastitis in Brazil. Br Vet J, 146, 443-448.
- 18. Lyon BR and Skurray R (1987): Antimicrobial resistance of Staphylococcus aureus: genetic basis. Microbiol Rev, 51, 88-134.
- 19. Maniatis R, Fritsh EF, Sambrook J (1982): A Laboratory Manual. Cold Spring Harbors, New York.
- 20. Matthews KR, Kumar SJ, O’Conner SA, Harmon RJ, Pankey JW, Fox LK, Oliver SP (1994): Genomic fingerprints of Staphylococcus aureus of bovine origin by polymerase chain reaction based DNA fingerpriting. Epidemiol Infect, 112, 177-186.
- 21. Matthews KR, Jayarao BM, Oliver SP (1992): Plasmid pattern analysis of Staphylococcus species isolated from bovine mammary secretions. J Dairy Sci, 75, 3318-3323.
- 22. Nei M (1972): Genetic distance between population. Am Nat, 106, 283-292.
- 23. Osmanağaoğlu Ö, Altınlar N, Saçılık SC, Çökmüş C, Akın A (2000): Identification of different Candida species isolated in various hospitals in Ankara by fungichrom test kit and their differentiation by SDS-PAGE. Turk J Med Sci, 30, 355-358
- 24. Pereira MS, Siqueira-Junior JP (1995): Antimicrobial drug resistance in Staphylococcus aureus from cattle in Brazil. Lett Appl Microbiol, 20, 391-395.
- 25. Saçılık SC, Osmanağaoğlu Ö, Palabıyıkoğlu İ, Bengisun JS, Çökmüş C (2000): Analysis of methicillin resistant Staphylococcus aureus isolates by polyacrylamid gel electrophoresis in an intensive care unit of İbni-Sina hospital. Turk J Med Sci, 30, 367-371.
- 26. Sordillo LM, Shafer-Weaver K, DeRosa D (1997): Immunobiology of the mammary gland. J Dairy Sci, 80, 1851-1865.
- 27. Struelens MJ, Deplano A, Godard C, Maes N, Serruys E (1992): Epidemiologic typing and delineation of genetic relatedness of methicillin-resistant Staphylococcus aureus by macrorestriction analysis of genomic DNA by using pulsed-field gel electrophoresis. J Clin Microbiol, 30, 2599-2605.
- 28. Tenover FC, Arbeit R, Archer G, Biddle J, Byrne S, Goering R, Hancock G, Hébert GA, Hill B, Hollis R, Jarvis WR, Kreiswirth B, Eisner W, Maslow J, McDougal LK, Miller JM, Mulligan M, Pfaller MA (1994): Comparison of traditional and molecular methods of typing isolates of Staphylococcus aureus. J Clin Microbiol, 32, 407-415.
- 29. Watts JL (1988): Etiological agents of bovine mastitis. Vet Microbiol, 16, 41-66.
- 30. Witte W, Marples RR, Richardson JF (1988): Complex typing of methicillin-resistant Staphylococcus aureus (MRSA). Zentralbl Bakteriol Mikrobiol Hyg A, 270, 76-82.
- 31. Younis A, Leitner G, Heller DE, Samra Z, Gadba R, Lubashevsky G, Chaffer M, Yadlin N, Winkler M, Saran A (2000): Phenotypic chracateristics of Staphylococcus aureus isolated from bovine mastitis in Israeli dairy herds. J Vet Med B Infect Dis Vet Public Health, 47, 591-597
Subklinik inek mastitislerinden izole edilen Staphylococcus aureus suşlarının protein paterni, antibiyotik direnci ve plazmid profili yönünden karakterizasyonu
Abstract
Hatay bölgesindeki subklinik inek mastitislerinden izole edilen toplam 50 Staphylococcus aureus suşunun protein paterni, antibiyotik dirençliliği ve plazmid profili yönünden karakterizasyonu yapıldı. S. aureus suşlarının SDS-PAGE ile analizinde moleküler ağırlığı 116 kDa arasında değişen 17-49 bant elde edildi. Elektroforetik protein bantlarının bilgisayar destekli numerikal analizi sonucunda S. aureus suşları 2 gruba ayrıldı. S. aureus suşları trimethoprim-sülfametoksazol, danofloksasin, amoksisilin-klavulanik asit, sulbaktam-ampisilin, eritromisin, linkomisin, sefoperazon, oksitetrasiklin, amoksisilin, streptomisin ve penisilin’e sırasıyla % 4, % 4, % 10, % 14, % 30, % 34, % 36, % 38, % 50, % 54 ve % 68 oranlarında dirençli bulundu. İncelenen izolatların 47’sinde, büyüklüğü 1.7->24 kb arasında değişen plazmidler saptandı
References
- 1. Aarestrop FM, Wegener HC, Rosdahl VT (1995): Evaluation of phenotypic and genotypic methods for epidemiological typing of Staphylococcus aureus isolates from bovine mastitis in Denmark. Vet Microbiol, 45, 139- 150.
- 2. Anderson DG, McKay LL (1983): Simple and rapid method for isolating large plasmid DNA from lactic Streptococci. Appl Environ Microbiol, 46, 549-552.
- 3. Bannerman TL, Hancock GA, Tenover FC, Miller JM (1995): Pulsed-field gel electrophoresis as a replacement for bacteriphage typing of Staphylococcus aureus. J Clin Microbiol, 33, 551-555.
- 4. Bauer AW, Kirby WM, Sherris JC, Turck M (1966): Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol, 45, 493-496.
- 5. Baumgartner A, Nicolet J, Eggimann M (1984): Plasmid profiles of Staphylococcus aureus causing bovine mastitis. J Appl Bacteriol, 56, 159-163.
- 6. Bushuk W, Hay RL, Larsen NG, Sara RG, Simmons LD, Sutton KH (1997): Effect of Mechanical Dough Development on the Extractability of Wheat Storage Proteins from Bread Dough. Cereal Chem, 74, 389-395.
- 7. Costas M, Holmes B, Sloss LL (1987): Numerical analysis of electrophoretic protein patterns of Providencia rustigianii strains from human diarrhoea and other sources. J Appl Bacteriol, 63, 319-328.
- 8. Costas M, Cookson BD, Talsania HG, Owen RJ (1989): Numerical analysis of electrophoretic protein patterns of methicillin-resistant strains of Staphylococcus aureus. J Clin Microbiol, 27, 2574-2581.
- 9. Demiralp H, Çelik S, Köksel H (2000): Effects of oxidizing agents and defatting on the electrophoretic patterns of flour proteins during dough mixing. Eur Food Res Technol, 211, 322-325.
- 10. Fitzgerald JR, Meaney WJ, Hartigan PJ, Smyth CJ, Kapur V (1997): Fine-structure molecular epidemiological analysis of Staphylococcus aureus recovered from cows. Epidemiol Infect, 119, 261-269.
- 11. Goh SH, Byrne SK, Zhang JL, Chow AW (1992): Molecular typing of Staphylococcus aureus on the basis of coagulase gene polymorphisms. J Clin Microbiol, 30, 1642-1645.
- 12. Kapur V, Sischo WM, Greer RS, Whittam TS, Musser JM (1995): Molecular population genetic analysis of Staphylococcus aureus recovered from cows. J Clin Microbiol, 33, 376-380.
- 13. Kishore L, Natarajan K, Babu LR (1996): Total soluble protein and membrane lipopolysaccharide profiles in differentiating Rhizobium isolates. Microbios, 86, 143-156.
- 14. Koneman EM, Allen SD, Dovell VR, Sommers HM, Winn WC (1997): Color Atlas and Textbook of Diagnostic Microbiology. JP Lippincott Comp, Philadelphia
- 15. Laemmli UK (1970): Cleavege of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-684.
- 16. Lange C, Cardoso M, Senczek D, Schwarz S (1999): Molecular subtyping of Staphylococcus aureus isolates from cases of bovine mastitis in Brazil. Vet Microbiol, 67, 127-141.
- 17. Lopes CA, Moreno G, Curi PR, Gottschalk AF, Modolo JR, Horacio A, Correa A, Pavan C (1990): Charecteristics of Staphylococcus aureus from subclinical bovine mastitis in Brazil. Br Vet J, 146, 443-448.
- 18. Lyon BR and Skurray R (1987): Antimicrobial resistance of Staphylococcus aureus: genetic basis. Microbiol Rev, 51, 88-134.
- 19. Maniatis R, Fritsh EF, Sambrook J (1982): A Laboratory Manual. Cold Spring Harbors, New York.
- 20. Matthews KR, Kumar SJ, O’Conner SA, Harmon RJ, Pankey JW, Fox LK, Oliver SP (1994): Genomic fingerprints of Staphylococcus aureus of bovine origin by polymerase chain reaction based DNA fingerpriting. Epidemiol Infect, 112, 177-186.
- 21. Matthews KR, Jayarao BM, Oliver SP (1992): Plasmid pattern analysis of Staphylococcus species isolated from bovine mammary secretions. J Dairy Sci, 75, 3318-3323.
- 22. Nei M (1972): Genetic distance between population. Am Nat, 106, 283-292.
- 23. Osmanağaoğlu Ö, Altınlar N, Saçılık SC, Çökmüş C, Akın A (2000): Identification of different Candida species isolated in various hospitals in Ankara by fungichrom test kit and their differentiation by SDS-PAGE. Turk J Med Sci, 30, 355-358
- 24. Pereira MS, Siqueira-Junior JP (1995): Antimicrobial drug resistance in Staphylococcus aureus from cattle in Brazil. Lett Appl Microbiol, 20, 391-395.
- 25. Saçılık SC, Osmanağaoğlu Ö, Palabıyıkoğlu İ, Bengisun JS, Çökmüş C (2000): Analysis of methicillin resistant Staphylococcus aureus isolates by polyacrylamid gel electrophoresis in an intensive care unit of İbni-Sina hospital. Turk J Med Sci, 30, 367-371.
- 26. Sordillo LM, Shafer-Weaver K, DeRosa D (1997): Immunobiology of the mammary gland. J Dairy Sci, 80, 1851-1865.
- 27. Struelens MJ, Deplano A, Godard C, Maes N, Serruys E (1992): Epidemiologic typing and delineation of genetic relatedness of methicillin-resistant Staphylococcus aureus by macrorestriction analysis of genomic DNA by using pulsed-field gel electrophoresis. J Clin Microbiol, 30, 2599-2605.
- 28. Tenover FC, Arbeit R, Archer G, Biddle J, Byrne S, Goering R, Hancock G, Hébert GA, Hill B, Hollis R, Jarvis WR, Kreiswirth B, Eisner W, Maslow J, McDougal LK, Miller JM, Mulligan M, Pfaller MA (1994): Comparison of traditional and molecular methods of typing isolates of Staphylococcus aureus. J Clin Microbiol, 32, 407-415.
- 29. Watts JL (1988): Etiological agents of bovine mastitis. Vet Microbiol, 16, 41-66.
- 30. Witte W, Marples RR, Richardson JF (1988): Complex typing of methicillin-resistant Staphylococcus aureus (MRSA). Zentralbl Bakteriol Mikrobiol Hyg A, 270, 76-82.
- 31. Younis A, Leitner G, Heller DE, Samra Z, Gadba R, Lubashevsky G, Chaffer M, Yadlin N, Winkler M, Saran A (2000): Phenotypic chracateristics of Staphylococcus aureus isolated from bovine mastitis in Israeli dairy herds. J Vet Med B Infect Dis Vet Public Health, 47, 591-597
There are 31 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery |
| Journal Section | Research Article |
| Authors | |
| Publication Date | March 1, 2006 |
| Published in Issue | Year 2006Volume: 53 Issue: 1 |