Ankara Üniversitesi Veteriner Fakültesi Dergisi

Abstract

The aim of this study is to determine the pharmacokinetic properties of approved medicines including enrofloxacin in broiler after single intravenous (IV) and orally administered at the dose level of 10 mg/kg body weight (bw) and to find out if there are differences in their pharmacokinetic profiles. In this study, 24 broiler chickens, 40 days-old, were used. Broiler chicks were obtained one-day-old and fed by fodder without any drug supplementation for 40 days. When they were 25 days-old, divided into 4 groups (as groups 1, 2A, 2B and 2C). Enrofloxacin was given IV to the animals in Group 1; Baytril, Enrolen and Hipralona Enro-S were administered Groups 2A, 2B and 2C via drinking water, respectively. Following the drug administration, blood samples were obtained from left v. cutenea ulnaris of chicken at 0.08 to 24 hours and collected with citrated syringes though a canula. The plasma was separated by centrifugition (3000 rpm, 10 minutes) and analyzed by agar-jel disc-diffussion method. Curve of plasma enrofloxacin concentration-time profile was characteristics of a two-compartmental-open model. There were significient differences between IV and orally administration in same pharmacokinetic parameters. Following the IV administration of enrofloxacin, some pharmacokinetic variables were as follows: k12=4.35±0.47 hours-1 ; k21=0.50±0.19 hours-1; k10=4.46±0.47 hours-1; AUC=41.8±3.1 µg.hours/ml; t1/2α=0.08±0.00 hours: t1/2β=9.62±0.36 hours; V1=0.66±0.39 L/kg; MRT=12.64±0.43 hours; Cl=8.13±4.44 ml/hour.kg. Following the administration of Baytril, Enrolen and Hipralona Enro-S via in drinking water, some pharmacokinetic variables were as follows, respectively: t1/2α=0.14±0.07 hours; 0.09±0.01 hours and 0.14±0.06 hours; t1/2β=17.32±1.69 hours, 5.33±0.21 hours and 34.65±2.72 hours; V1=0.20±0.09 L/kg, 0.50±0.27 L/kg, and 1.68±0.08 L/kg; AUC=30.7±4.8 µg.hours/ml, 41.3±3.4 µg.hours/ml and 31.2±3.5 µg.hours/ml; F=%73.44, %98.8 and %74.64; MRT=18.02±2.48 hours, 17.00±2.32 hours and 15.06±6.98 hours; Cl=4.31±1.87 ml/hours.kg, 6.15±3.14 ml/hours.kg and 0.33±0.03 ml/hours.kg. The results of this study pointed out that all these approved medicines were absorbed highly from the gastro-intestinal tract in boiler and therefore their bioavailability was high. The best absorbtion and high level drug concentration in plasma were obtained via in drinking water administration by Enrolen