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Aflatoksin M1’in yoğurt bakterilerine bağlanma yeteneği

Year 2004, Volume: 51 Issue: 3, 195 - 198, 01.09.2004

Belgin Sarımehmetoğlu Özlem Küplülü

Abstract

Bu çalışmada, kontamine PBS’de AFM1’in bağlanma yeteneği araştırıldı. Bu amaçla, Lactobacillus delbrueckii subsp. bulgaricus CH-2 ve Streptococcus thermophilus ST-36 bakterileri kullanıldı. AFM1’in bu iki bakteriye bağlanma yeteneği, aynı zamanda kontamine sütte ve kontamine sütten yapılan yoğurtta da araştırıldı. Çalışmada, ELISA yöntemi kullanıldı. Lactobacillus delbrueckii subsp. bulgaricus CH-2’nin AFM1’i PBS’de % 18.70, sütte % 27.56; Streptococcus thermophilus ST-36’nın ise PBS’de % 29.42 ve sütte % 39.16 düzeylerinde bağlama yeteneğinde olduğu belirlendi. Yoğurtta ise bağlanma en düşük düzeyde saptandı( % 14.82). Sonuç olarak, gerek PBS’de gerekse sütte Streptococcus thermophilus’un bağlama yeteneği, Lactobacillus delbrueckii subsp. bulgaricus CH-2’den daha yüksek bulundu. Aynı zamanda her iki bakterinin de PBS’e göre sütte daha fazla bağlama yeteneği olduğu saptandı. Elde edilen bulgulara göre, bu çalışmada kullanılan spesifik yoğurt bakterilerinin AFM1’i sütten uzaklaştırma yeteneğinde olduğu belirlendi

Keywords

Aflatoksin M1 bağlanma yoğurt bakterileri

References

  • Ahokas J, El-Nezami H, Kankaanpaa P, Mykkanen H, Salminen S (1998): A pilot clinical study examining the ability of a mixture of Lactobacillus and Propioni- bacterium to remove aflatoxin from the gastrointestinal tract of healthy Egyptan volunteers. Satellite Meeting IU- TOUX VIIIth International Congress of Toxicology. 568., 2-4 July 1998, Toulouse France.
  • Basappa SC, Shantha T (1996): Methods for detoxification of aflatoxins in foods and feeds- A critical appraisal. J Food Sci Technol, 33, 95-107.
  • Brackett RE, Marth EH (1982a): Association of aflatoxin M1 with casein. Z Lebensm Unters Forsch, 174, 439-441.
  • Brackett RE, Marth EH (1982b): Fate of aflatoxin M1 in cheddar cheese and in process cheese spread. J Food Prot, 45, 549-552.
  • Concon JM (1988): Mold and Mycotoxin Contamination of Food Products. Food Toxicology. Part B: Contaminants and Additives.667-743. Marcel Dekker, Inc. New York.
  • El-Nezami H, Kankaanpaa P, Salminen S., Ahokas J (1998a): Physicochemical alterations enhance the ability of dairy strains of lactic acid bacteria to remove aflatoxin from contaminated media. J Food Prot, 61, 466-468.
  • El-Nezami, H, Kankaanpaa, P, Salminen, S, Ahokas J (1998b): Ability of dairy strains of lactic acid bacteria to bind food carcinocens. Food Chem Toxicol, 36, 321-326.
  • Frazier WC, Westhoff DC (1988): Food-borne Poisonings, Infections and Intoxications: Nonbacterial. Food Microbiology. 4th ed. Chapter: 25, 440- 449.,McGraw-Hill Book Company, New York.
  • Galvano F, Galofaro V, Galvano G. (1996): Occurrence and stability, of aflatoxin M1 in milk and milk products: A worldwide review. J Food Prot, 59, 1079-1090.
  • Galvano F, Galofaro V, Ritienis M, Bognanno A, Angelis A, Galvano G (2001): Survey of the occurrence of aflatoxin M1 in dairy products marketed in Italy: second year of observation. Food Add Contam, 18, 644-646.
  • Kane A, Badiop N, Diack TS, Philips TD (1998): Different technolotical processes of removing aflatoxin B1 from crude peanut oil. Revue Mèd Vèt, 149, 565.
  • Meerarani S, Ramadass P, Padmanaban VD, Nachimutu K (1997): Incidence of aflatoxin M1 in milk samples around Chennai (Madras) city. J Food Sci Technol, 34, 506-508.
  • Munksgaard L, Larsen J, Werner H, Andersen PH, Viuf PT (1987): Carry over of aflatoxin from cows’ feed to milk and milk products. Milchwissensc, 42, 165-167.
  • Oatley JT, Rarick MD, Ji GE, Linz JE (2000): Binding of aflatoxin B1 to bifidobacteria in vitro. J Food Prot, 63, 1133-1136.
  • Oruc H, Sonal S (2001): Determination of aflatoxin M1 levels in cheese and milk consumed in Bursa, Turkey. Vet Hum Toxicol, 43, 292-293.
  • Peltonen K, El-Nezami H, Haskard C, Ahokas J, Salminen S (2001): Aflatoxin B1 binding by dairy strains of lactic acid bacteria and bifidobacteria. J Dairy Sci 84, 2152-2156.
  • Pierides M, El-Nezami H, Peltonen K, Salminen S, Ahokas J (2000): Ability of dairy strains of lactic acid bacteria to bind aflatoxin M1 in a food model. J Food Prot, 63, 645-650.
  • Pittet, A (1998): Natural occurrence of mycotoxins in foods and feeds-an updated review. Revue Mèd Vèt 149, 479- 492.
  • Rasic JL, Skrinjar M, Markov S ( 1991): Decrease of aflatoxin B1 in yoghurt and acidified milks. Mycopathologia, 113, 117-119.
  • Rustom, IYS (1997): Aflatoxin in food and feed: occurrence, legislation and inactivation by phycical methods. Food Chem, 59, 57-67.
  • Seyrek K ( 2001): Türk Silahlı Kuvvetleri’ne bağlı birlik- lerde tüketilen beyaz peynirlerdeki aflatoksin M1 seviyesi- nin ELISA metodu ile saptanması. Vet Hek Der Derg, 72, 55-58.
  • Srivastava VP, Bu-Abbas A, Al-Johar W, Al-Mufti S, Siddiqui MKJ (2001): Aflatoxin M1 contamination in commercial samples of milk and dairy products in Kuwait. Food Add Contam, 18, 993-997.
  • Steyn PS (1998): The biosynthesis of mycotoxins. Revue Mèd Vèt, 149, 469-478.
  • Stolof L (1980): Aflatoxin M in perspective. J Food Prot, 43, 226-230.
  • Tabata S, Kamimura A, Ibe H, Hasimoto H, Ida M, Tamura Y, Nishima T (1993): Aflatoxin contamination in foods and foodstuffs in Tokyo: 1986-1990. J AOAC Int, 76, 32-35.
  • Van Egmond HP, Paulch WE, Veringa HA, Schuller PL (1977): The effect of processing on the aflatoxin M1 content of milk and milk products., Arch. Inst. Pasteur (Tunis). 381-390. In: Applebaum, R. S., Brackett, R. E., Wiseman, D. W., Marth, E. H. (1982): Aflatoxin: Toxicity of dairy cattle and occurrence in milk and milk products A Review J Food Protec, 45, 752-777.
  • Webley DJ, Jackson KL, Mullins JG (1997): Mycotoxins in food: a review of recent analyses. Food Australia, 49, 375-379.

Binding ability of aflatoxin M1 to yoghurt bacteria

Year 2004, Volume: 51 Issue: 3, 195 - 198, 01.09.2004

Belgin Sarımehmetoğlu Özlem Küplülü

Abstract

Aflatoxin M1 (AFM1) is a highly toxic compound found in milk. Several microorganisms have been previously reported to bind or degrade AFM1 from liquid media. This study was performed to assess the binding of AFM1 in contaminated phosphate buffered saline (PBS). Lactobacillus delbrueckii subsp. bulgaricus CH-2 and Streptococcus thermophilus ST-36 were used for this purpose. Removal activities of two strains were also assessed using contaminated reconstituted milk and contaminated yoghurt made from reconstituted milk. ELISA procedure was used in this study Lactobacillus delbrueckii subsp. bulgaricus CH-2 bound in PBS at 18.70% and in milk at 27.56% while Streptococcus thermophilus ST-36 bound in PBS at 29.42% and in milk at 39.16%. AFM1 was bound at the level of merely 14.82% in yogurt. The results indicated that binding ability of Streptococcus thermophilus ST-36 was higher than that of Lactobacillus delbrueckii subsp. bulgaricus CH-2 in both PBS and reconstituted milk. Both of microorganisms bound higher in milk than in PBS. Also, AFM1 binding levels were at least level in yoghurt (%14.82). These findings supported that specific yoghurt bacteria used in this study can offer decontaminating AFM1 from milk

Keywords

Aflatoxin M1 binding yoghurt bacteria

References

  • Ahokas J, El-Nezami H, Kankaanpaa P, Mykkanen H, Salminen S (1998): A pilot clinical study examining the ability of a mixture of Lactobacillus and Propioni- bacterium to remove aflatoxin from the gastrointestinal tract of healthy Egyptan volunteers. Satellite Meeting IU- TOUX VIIIth International Congress of Toxicology. 568., 2-4 July 1998, Toulouse France.
  • Basappa SC, Shantha T (1996): Methods for detoxification of aflatoxins in foods and feeds- A critical appraisal. J Food Sci Technol, 33, 95-107.
  • Brackett RE, Marth EH (1982a): Association of aflatoxin M1 with casein. Z Lebensm Unters Forsch, 174, 439-441.
  • Brackett RE, Marth EH (1982b): Fate of aflatoxin M1 in cheddar cheese and in process cheese spread. J Food Prot, 45, 549-552.
  • Concon JM (1988): Mold and Mycotoxin Contamination of Food Products. Food Toxicology. Part B: Contaminants and Additives.667-743. Marcel Dekker, Inc. New York.
  • El-Nezami H, Kankaanpaa P, Salminen S., Ahokas J (1998a): Physicochemical alterations enhance the ability of dairy strains of lactic acid bacteria to remove aflatoxin from contaminated media. J Food Prot, 61, 466-468.
  • El-Nezami, H, Kankaanpaa, P, Salminen, S, Ahokas J (1998b): Ability of dairy strains of lactic acid bacteria to bind food carcinocens. Food Chem Toxicol, 36, 321-326.
  • Frazier WC, Westhoff DC (1988): Food-borne Poisonings, Infections and Intoxications: Nonbacterial. Food Microbiology. 4th ed. Chapter: 25, 440- 449.,McGraw-Hill Book Company, New York.
  • Galvano F, Galofaro V, Galvano G. (1996): Occurrence and stability, of aflatoxin M1 in milk and milk products: A worldwide review. J Food Prot, 59, 1079-1090.
  • Galvano F, Galofaro V, Ritienis M, Bognanno A, Angelis A, Galvano G (2001): Survey of the occurrence of aflatoxin M1 in dairy products marketed in Italy: second year of observation. Food Add Contam, 18, 644-646.
  • Kane A, Badiop N, Diack TS, Philips TD (1998): Different technolotical processes of removing aflatoxin B1 from crude peanut oil. Revue Mèd Vèt, 149, 565.
  • Meerarani S, Ramadass P, Padmanaban VD, Nachimutu K (1997): Incidence of aflatoxin M1 in milk samples around Chennai (Madras) city. J Food Sci Technol, 34, 506-508.
  • Munksgaard L, Larsen J, Werner H, Andersen PH, Viuf PT (1987): Carry over of aflatoxin from cows’ feed to milk and milk products. Milchwissensc, 42, 165-167.
  • Oatley JT, Rarick MD, Ji GE, Linz JE (2000): Binding of aflatoxin B1 to bifidobacteria in vitro. J Food Prot, 63, 1133-1136.
  • Oruc H, Sonal S (2001): Determination of aflatoxin M1 levels in cheese and milk consumed in Bursa, Turkey. Vet Hum Toxicol, 43, 292-293.
  • Peltonen K, El-Nezami H, Haskard C, Ahokas J, Salminen S (2001): Aflatoxin B1 binding by dairy strains of lactic acid bacteria and bifidobacteria. J Dairy Sci 84, 2152-2156.
  • Pierides M, El-Nezami H, Peltonen K, Salminen S, Ahokas J (2000): Ability of dairy strains of lactic acid bacteria to bind aflatoxin M1 in a food model. J Food Prot, 63, 645-650.
  • Pittet, A (1998): Natural occurrence of mycotoxins in foods and feeds-an updated review. Revue Mèd Vèt 149, 479- 492.
  • Rasic JL, Skrinjar M, Markov S ( 1991): Decrease of aflatoxin B1 in yoghurt and acidified milks. Mycopathologia, 113, 117-119.
  • Rustom, IYS (1997): Aflatoxin in food and feed: occurrence, legislation and inactivation by phycical methods. Food Chem, 59, 57-67.
  • Seyrek K ( 2001): Türk Silahlı Kuvvetleri’ne bağlı birlik- lerde tüketilen beyaz peynirlerdeki aflatoksin M1 seviyesi- nin ELISA metodu ile saptanması. Vet Hek Der Derg, 72, 55-58.
  • Srivastava VP, Bu-Abbas A, Al-Johar W, Al-Mufti S, Siddiqui MKJ (2001): Aflatoxin M1 contamination in commercial samples of milk and dairy products in Kuwait. Food Add Contam, 18, 993-997.
  • Steyn PS (1998): The biosynthesis of mycotoxins. Revue Mèd Vèt, 149, 469-478.
  • Stolof L (1980): Aflatoxin M in perspective. J Food Prot, 43, 226-230.
  • Tabata S, Kamimura A, Ibe H, Hasimoto H, Ida M, Tamura Y, Nishima T (1993): Aflatoxin contamination in foods and foodstuffs in Tokyo: 1986-1990. J AOAC Int, 76, 32-35.
  • Van Egmond HP, Paulch WE, Veringa HA, Schuller PL (1977): The effect of processing on the aflatoxin M1 content of milk and milk products., Arch. Inst. Pasteur (Tunis). 381-390. In: Applebaum, R. S., Brackett, R. E., Wiseman, D. W., Marth, E. H. (1982): Aflatoxin: Toxicity of dairy cattle and occurrence in milk and milk products A Review J Food Protec, 45, 752-777.
  • Webley DJ, Jackson KL, Mullins JG (1997): Mycotoxins in food: a review of recent analyses. Food Australia, 49, 375-379.
There are 27 citations in total.

Details

Primary Language English
Journal Section Research Article
Authors

Belgin Sarımehmetoğlu

Özlem Küplülü

Publication Date September 1, 2004
Published in Issue Year 2004Volume: 51 Issue: 3

Cite

APA Sarımehmetoğlu, B., & Küplülü, Ö. (2004). Binding ability of aflatoxin M1 to yoghurt bacteria. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 51(3), 195-198.
AMA Sarımehmetoğlu B, Küplülü Ö. Binding ability of aflatoxin M1 to yoghurt bacteria. Ankara Univ Vet Fak Derg. September 2004;51(3):195-198.
Chicago Sarımehmetoğlu, Belgin, and Özlem Küplülü. “Binding Ability of Aflatoxin M1 to Yoghurt Bacteria”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 51, no. 3 (September 2004): 195-98.
EndNote Sarımehmetoğlu B, Küplülü Ö (September 1, 2004) Binding ability of aflatoxin M1 to yoghurt bacteria. Ankara Üniversitesi Veteriner Fakültesi Dergisi 51 3 195–198.
IEEE B. Sarımehmetoğlu and Ö. Küplülü, “Binding ability of aflatoxin M1 to yoghurt bacteria”, Ankara Univ Vet Fak Derg, vol. 51, no. 3, pp. 195–198, 2004.
ISNAD Sarımehmetoğlu, Belgin - Küplülü, Özlem. “Binding Ability of Aflatoxin M1 to Yoghurt Bacteria”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 51/3 (September 2004), 195-198.
JAMA Sarımehmetoğlu B, Küplülü Ö. Binding ability of aflatoxin M1 to yoghurt bacteria. Ankara Univ Vet Fak Derg. 2004;51:195–198.
MLA Sarımehmetoğlu, Belgin and Özlem Küplülü. “Binding Ability of Aflatoxin M1 to Yoghurt Bacteria”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 51, no. 3, 2004, pp. 195-8.
Vancouver Sarımehmetoğlu B, Küplülü Ö. Binding ability of aflatoxin M1 to yoghurt bacteria. Ankara Univ Vet Fak Derg. 2004;51(3):195-8.