Diagnosis of infectious bursal disease by immunoperoxidase technique

Tolga Güvenç; Rıfkı Hazıroğlu; Murat Yarım; Recai Tunca

İnfeksiyöz bursal hastalığın immunoperoksidaz tekniği ile tanısı

Year 2004, Volume: 51 Issue: 3, 189 - 193, 01.09.2004

Tolga Güvenç Rıfkı Hazıroğlu Murat Yarım Recai Tunca

Abstract

İnfeksiyöz bursal hastalığın tanısı, immunoperoksidaz metodu kullanılarak doğal olarak infekte 21 günlük civcivlerde incelendi. Viral antijenler bursa Fabricius’daki makrofaj ve lenfositlerde gözlendi. Çalışmada kullanılan metodun duyarlı ve spesifik olduğu ve hastalığın tanısında kullanılabileceği saptandı

Keywords

İmmunohistokimya, infeksiyöz bursal hastalık, poliklonal antikor

References

Cruz-Coy JS, Giambrone JJ, Hoerr FJ (1993): İmmu- nohistochemical detection of infectious bursal disease vi- rus in formalin-fixed, paraffin-embedded chicken tissues using monoclonal antibody. Avian Dis, 37, 577-581.
Eterradosi A, Picault J, Drouin P (1992): Pathogenicity and preliminary antigenic characterisation of six infec- tious bursal disease virus strains isolated in France from acute outbreaks. J Vet Med, 39, 683-691.
Fernandes A, Martin M, Sierra M (1989): Immunohis- tological identification of both infectious bursal and Marek virus antigens in the bursa of Fabricius. Dtsch Tierarzl Wschr, 96, 157-160.
Haziroglu R, Maeda M, Nakamura K, Haritani M, Narita M (1988): Diagnosis of infectious bursal disease (IBD) by immunofluoresence. Ankara Univ Vet Fak Derg, 25, 289-298.
Jonson L, Engstrom B (1986): Immunocytochemical detection of infectious bursal disease and infectious bron- chitis viral antigens in fixed, paraffin embedded chicken tissues. Avian Pathol, 15, 385-393.
Kovacevic SA, Gagic M, Lazic S, Kovacevic M (1999): Immunohistochemical detection of infectious bursal dis- ease virus antigen in the bursa of fabricius of experimen- tally infected chickens. Acta Vet. (Beograd), 49, 13-20.
Lukert PD, Saif YM (1997): Infectious bursal disease. 721-738. In: BW Calnek, H J Barnes, CW Beard, McDou- gald L R, Saif M Y (Eds.): Disease of Poultry. Iowa State University Press, Ames, Iowa.
Tanimura N, Tsukamoto K, Nakamura, K, Narita M, Maeda M (1995): Association between pathogenicity of infectious bursal disease virus and viral antigen distribu- tion detected by immunohistochemistry. Avian Dis, 39, 9- 20. Geliş tarihi: 31.10.2003 / Kabul tarihi: 16.02.2004

Diagnosis of infectious bursal disease by immunoperoxidase technique

Year 2004, Volume: 51 Issue: 3, 189 - 193, 01.09.2004

Tolga Güvenç Rıfkı Hazıroğlu Murat Yarım Recai Tunca

Abstract

The diagnosis of infectious bursal disease of naturally infected 21-day-old chickens was investigated with immunoperoxidase method. Viral antigens were observed in the macrophages and lymphocytes of the bursa of Fabricius. It was observed that the method was sensitive and specific and it could be used in the diagnosis of this disease

Keywords

Immunohistochemistry, infectious bursal disease, polyclonal antibody

References

Cruz-Coy JS, Giambrone JJ, Hoerr FJ (1993): İmmu- nohistochemical detection of infectious bursal disease vi- rus in formalin-fixed, paraffin-embedded chicken tissues using monoclonal antibody. Avian Dis, 37, 577-581.
Eterradosi A, Picault J, Drouin P (1992): Pathogenicity and preliminary antigenic characterisation of six infec- tious bursal disease virus strains isolated in France from acute outbreaks. J Vet Med, 39, 683-691.
Fernandes A, Martin M, Sierra M (1989): Immunohis- tological identification of both infectious bursal and Marek virus antigens in the bursa of Fabricius. Dtsch Tierarzl Wschr, 96, 157-160.
Haziroglu R, Maeda M, Nakamura K, Haritani M, Narita M (1988): Diagnosis of infectious bursal disease (IBD) by immunofluoresence. Ankara Univ Vet Fak Derg, 25, 289-298.
Jonson L, Engstrom B (1986): Immunocytochemical detection of infectious bursal disease and infectious bron- chitis viral antigens in fixed, paraffin embedded chicken tissues. Avian Pathol, 15, 385-393.
Kovacevic SA, Gagic M, Lazic S, Kovacevic M (1999): Immunohistochemical detection of infectious bursal dis- ease virus antigen in the bursa of fabricius of experimen- tally infected chickens. Acta Vet. (Beograd), 49, 13-20.
Lukert PD, Saif YM (1997): Infectious bursal disease. 721-738. In: BW Calnek, H J Barnes, CW Beard, McDou- gald L R, Saif M Y (Eds.): Disease of Poultry. Iowa State University Press, Ames, Iowa.
Tanimura N, Tsukamoto K, Nakamura, K, Narita M, Maeda M (1995): Association between pathogenicity of infectious bursal disease virus and viral antigen distribu- tion detected by immunohistochemistry. Avian Dis, 39, 9- 20. Geliş tarihi: 31.10.2003 / Kabul tarihi: 16.02.2004

There are 8 citations in total.

Details

Primary Language	English
Journal Section	Research Article
Authors	Tolga Güvenç Rıfkı Hazıroğlu Murat Yarım Recai Tunca
Publication Date	September 1, 2004
Published in Issue	Year 2004Volume: 51 Issue: 3

Cite

APA	Güvenç, T., Hazıroğlu, R., Yarım, M., Tunca, R. (2004). Diagnosis of infectious bursal disease by immunoperoxidase technique. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 51(3), 189-193.
AMA	Güvenç T, Hazıroğlu R, Yarım M, Tunca R. Diagnosis of infectious bursal disease by immunoperoxidase technique. Ankara Univ Vet Fak Derg. September 2004;51(3):189-193.
Chicago	Güvenç, Tolga, Rıfkı Hazıroğlu, Murat Yarım, and Recai Tunca. “Diagnosis of Infectious Bursal Disease by Immunoperoxidase Technique”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 51, no. 3 (September 2004): 189-93.
EndNote	Güvenç T, Hazıroğlu R, Yarım M, Tunca R (September 1, 2004) Diagnosis of infectious bursal disease by immunoperoxidase technique. Ankara Üniversitesi Veteriner Fakültesi Dergisi 51 3 189–193.
IEEE	T. Güvenç, R. Hazıroğlu, M. Yarım, and R. Tunca, “Diagnosis of infectious bursal disease by immunoperoxidase technique”, Ankara Univ Vet Fak Derg, vol. 51, no. 3, pp. 189–193, 2004.
ISNAD	Güvenç, Tolga et al. “Diagnosis of Infectious Bursal Disease by Immunoperoxidase Technique”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 51/3 (September 2004), 189-193.
JAMA	Güvenç T, Hazıroğlu R, Yarım M, Tunca R. Diagnosis of infectious bursal disease by immunoperoxidase technique. Ankara Univ Vet Fak Derg. 2004;51:189–193.
MLA	Güvenç, Tolga et al. “Diagnosis of Infectious Bursal Disease by Immunoperoxidase Technique”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 51, no. 3, 2004, pp. 189-93.
Vancouver	Güvenç T, Hazıroğlu R, Yarım M, Tunca R. Diagnosis of infectious bursal disease by immunoperoxidase technique. Ankara Univ Vet Fak Derg. 2004;51(3):189-93.