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Köpek derisindeki mast hücreleri: dağılım,yoğunluk, heterojenite ve tespit tekniklerinin etkisi

Year 2005, Volume: 52 Issue: 1, 7 - 12, 01.03.2005

Reşat Nuri Aştı Arif Kurtdede Reşat Nuri Aştı Emel Ergün Murat Güzel

https://doi.org/10.1501/Vetfak_0000000022

Abstract

Bu araştırma sağlıklı erişkin köpeklerde farklı deri bölgelerindeki mast hücrelerinin yerleşim yerlerini, morfolojilerini ve sayısal dağılımlarını incelemek amacıyla yapıldı. Çalışmada materyal olarak 10 adet sağlıklı köpeklerin yanak, kulak kepçesi, kaburga ve but bölgelerinden alınan deri örnekleri kullanıldı. Bulgularımız, alcian blue / safranin O ile boyanmasına ve formaldehit tespitine duyarlılığına göre köpek derisinde mast hücre heterojenitesini gösterdi. Mast hücreleri bu parametrelerin dört kombinasyonu ile tanımlandı: Formole duyarlı sadece alcian blue-pozitif granüller içeren mast hücreleri, formole dirençli sadece alcian blue-pozitif granüller içeren mast hücreleri, formole dirençli sadece safranin O-pozitif granüller içeren mast hücreleri ve formole dirençli hem safranin O-pozitif hem de alcian blue-pozitif mikst granüller içeren mast hücreleri. Toluidin blue ile boyanmış kesitlerde dört bölgede de IFAA tespitli dokuların % 10 formolle tespit edilenlere göre daha fazla mast hücresi içerdiği dikkati çekti. Bu iki tespit arasında mm²’de mast hücre sayı ortalamalarındaki farklılığın istatistiksel olarak önemli (p0.05) iken, diğer üç bölgede istatistiksel fark önemli (p

Keywords

Dağılım heterojenite köpek derisi mast hücresi

References

  • Auxilia ST, Hill PB (2000): Mast cell distribution, epidermal thickness and hair follicle density in normal ca- nine skin: possible explanations for the predilection sites of atopic dermatitis? Vet Dermatol, 11, 247-254.
  • Becker AB, Chung KF, McDonald DM, Lazarus SC, Frick OL, Gold WM (1985): Mast cell heterogeneity in dog skin. Anat Rec, 213, 477-480.
  • Becker AB, Chung KF, McDonald DM, Lazarus SC, Frick OL, Gold WM (1986): Cutaneous mast cell heterogeneity, response to antigen in atopic dogs. J Allergy Clin Immunol, 78, 937-942.
  • Benyon RC (1989): The human skin mast cell. Clin Exp Allergy, 19, 375-387.
  • Bienenstock J, Befus AD, Pearce F, Denburg J, Goodacre R (1982): Mast cell heterogeneity: derivation and function, with emphasis on the intestine. J Allergy Clin Immunol, 70, 407-412.
  • Böck P (1989): Romeis Mikroskopische Tecknik. 17. Aufl. Urban und Schwarzenberg München.
  • Chen W, Alley MR, Manktelow BW, Davey P (1990a): Mast cells in the ovine lower respiratory tract: heterogeneity, morphology and density. Int Arch Allergy Appl Immunol, 93, 99-106.
  • Chen W, Alley MR, Manktelow BW, Slack P (1990b): Mast cells in the bovine lower respiratory tract: morpho- logy, density and distribution. Br Vet J, 146, 425-435.
  • Cowen T, Trigg P, Eady RAJ (1979): Distribution of mast cells in human dermis: development of a mapping technique. Brit J Dermatol, 100, 635-641.
  • Eady RAJ, Cowen T, Marshall TF, Plummer V, Greaves MW (1979): Mast cell population density, blood vessel density and histamine content in normal human skin. Brit J Dermatol, 100, 623-633.
  • El Sayed S, Dyson M (1993): Histochemical heterogeneity of mast cells in rat dermis. Biotech Histochem, 68, 326-332.
  • Emerson JL, Cross RF (1965): The distribution of mast cells in normal canine skin. Am J Vet Res, 26,1379-1382.
  • Enerbäck L (1966a): Mast cells in rat gastrointestinal mucosa. 1. effects of fixation. Acta Path Microbiol Scandinav, 66, 289-302.
  • Enerbäck L (1966b): Mast cells in rat gastrointestinal mucosa. 2. dye-binding and metachromatic properties. Acta Path Microbiol Scandinav, 66, 303-312.
  • Eren Ü (2000): Köpek derisinde mast hücreleri. Ankara Üniv Vet Fak Derg, 47, 167-175.
  • Foster AP (1994): A study of the number and distribution of cutaneous mast cells with disease not affecting the skin. Vet Dermatol, 5, 17-20.
  • Gordon JR, Parris RB, Galli JS (1990): Mast cells as a source of multifunctional cytokines. Immunol Today, 11, 458-464.
  • Kurtdede N, Yörük M (1995): Tavuk ve bıldırcın deri- sinde mast hücrelerinin morfolojik ve histometrik incelen- mesi. Ankara Üniv Vet Fak Derg, 42, 77-83.
  • Lehmann EL (1998): Non parametrics statistical methods based on ranks. First edition, Prentice-Hall, Inc. Simon&Schuster/ A Viacom Company Upper Saddle River, New Jersey.
  • Marshall JS, Bienenstock J (1994): The role of mast cells in inflammatory reactions of the airways, skin and intestine. Curr Opin Immunol, 6, 853-859.
  • Marshall JS, Ford GP, Bell EB (1987): Formalin sensitivity and differential staining of mast cells in human dermis. Br J Dermatol, 117, 29-36.
  • Mikhail GR, Miller-Milinska A (1964): Mast cell population in human skin. J Invest Dermatol, 43, 249-254.
  • Persinger MA, Lapage P, Simard JP, Barker GH (1983): Mast cell number in incisional wounds in rat skin as a function of distance, time and treatment. Br J Derma- tol, 108, 179-187.
  • Schwartz LB, Austen KF (1984): Structure and function of the chemical mediators of mast cells. Prog Allergy, 34, 271-321.
  • Scott DW, Miller WH, Griffin CE (1995): Muller and Kirk's small animal dermatology. 5th Edition,W. B. Saunders Company, Philadelphia, 488-500.
  • Shanahan F, Macniven I, Dyck N, Denburg JA, Bienenstock J, Befus AD (1987): Human lung mast cells: distribution and abundance of histochemicaly distinct subpopulations. lnt Arch Allergy Appl Immunol, 83, 329- 331.
  • Skaper SD, Pollock M, Facci L (2001): Mast cells differentially express and release active high molecular weight neurotrophins. Brain Res Mol Brain Res, 97, 177- 185.
  • Walton S, DeSouza EJ (1983): Variation in mast cell numbers in psoriasis and lichen planus: comparisons with normal skin. Dermatologica, 166, 236-239.
  • Warton A, Papadimitriou JM, Goldie RG, Paterson JW (1986): An ultrastructural study of mast cells in the alveolar wall of normal and asthmatic lung. Aust Exp Biol Med Sci, 64, 435-444. Geliş tarihi: 08.12.2003 / Kabul tarihi: 02.04.2004 Corresponding address: Prof. Dr. Reşat Nuri Aştı Ankara Üniversitesi Veteriner Fakültesi Histoloji-Embriyoloji Anabilim Dalı 06110 Dışkapı, Ankara e-mail: resatnuriasti@hotmail.com

Mast cells in the dog skin: distribution, density, heterogeneity and influence of fixation techniques

Year 2005, Volume: 52 Issue: 1, 7 - 12, 01.03.2005

Reşat Nuri Aştı Arif Kurtdede Reşat Nuri Aştı Emel Ergün Murat Güzel

https://doi.org/10.1501/Vetfak_0000000022

Abstract

This study was conducted with the aim of examining the location, the morphology, and numerical distribution of mast cells in the different areas of the healthy adult dog skin. Skin samples taken from the cheek, pinna, thorax and thigh areas of 10 healthy dogs were used as the study material. The results show the existence of mast cell heterogeneity in dog skin with respect to staining with alcian blue / safranin O and formaldehyde fixation sensitivity. Mast cells characterized by four combination of these parameters were observed: Mast cells containing only alcian blue-positive granules which were formalin-sensitive, mast cells containing only alcian blue-positive granules which were formalin-resistant, mast cells containing only safranin O-positive granules which were formalin resistant and mast cells containing a mixture of alcian blue-positive and safranin O-positive granules which were formalin resistant. With toluidine blue staining method, it was founded that IFAA fixed tissues were contained more mast cells than those tissues fixed with the 10% formaldehyde in all four regions. The difference in average number of mast cells per mm² between the IFAA and the 10% formaldehyde fixed tissues was statistically significant (p0.05), in the other three areas, statistically significant differences (p

Keywords

Distribution dog skin heterogeneity mast cell

References

  • Auxilia ST, Hill PB (2000): Mast cell distribution, epidermal thickness and hair follicle density in normal ca- nine skin: possible explanations for the predilection sites of atopic dermatitis? Vet Dermatol, 11, 247-254.
  • Becker AB, Chung KF, McDonald DM, Lazarus SC, Frick OL, Gold WM (1985): Mast cell heterogeneity in dog skin. Anat Rec, 213, 477-480.
  • Becker AB, Chung KF, McDonald DM, Lazarus SC, Frick OL, Gold WM (1986): Cutaneous mast cell heterogeneity, response to antigen in atopic dogs. J Allergy Clin Immunol, 78, 937-942.
  • Benyon RC (1989): The human skin mast cell. Clin Exp Allergy, 19, 375-387.
  • Bienenstock J, Befus AD, Pearce F, Denburg J, Goodacre R (1982): Mast cell heterogeneity: derivation and function, with emphasis on the intestine. J Allergy Clin Immunol, 70, 407-412.
  • Böck P (1989): Romeis Mikroskopische Tecknik. 17. Aufl. Urban und Schwarzenberg München.
  • Chen W, Alley MR, Manktelow BW, Davey P (1990a): Mast cells in the ovine lower respiratory tract: heterogeneity, morphology and density. Int Arch Allergy Appl Immunol, 93, 99-106.
  • Chen W, Alley MR, Manktelow BW, Slack P (1990b): Mast cells in the bovine lower respiratory tract: morpho- logy, density and distribution. Br Vet J, 146, 425-435.
  • Cowen T, Trigg P, Eady RAJ (1979): Distribution of mast cells in human dermis: development of a mapping technique. Brit J Dermatol, 100, 635-641.
  • Eady RAJ, Cowen T, Marshall TF, Plummer V, Greaves MW (1979): Mast cell population density, blood vessel density and histamine content in normal human skin. Brit J Dermatol, 100, 623-633.
  • El Sayed S, Dyson M (1993): Histochemical heterogeneity of mast cells in rat dermis. Biotech Histochem, 68, 326-332.
  • Emerson JL, Cross RF (1965): The distribution of mast cells in normal canine skin. Am J Vet Res, 26,1379-1382.
  • Enerbäck L (1966a): Mast cells in rat gastrointestinal mucosa. 1. effects of fixation. Acta Path Microbiol Scandinav, 66, 289-302.
  • Enerbäck L (1966b): Mast cells in rat gastrointestinal mucosa. 2. dye-binding and metachromatic properties. Acta Path Microbiol Scandinav, 66, 303-312.
  • Eren Ü (2000): Köpek derisinde mast hücreleri. Ankara Üniv Vet Fak Derg, 47, 167-175.
  • Foster AP (1994): A study of the number and distribution of cutaneous mast cells with disease not affecting the skin. Vet Dermatol, 5, 17-20.
  • Gordon JR, Parris RB, Galli JS (1990): Mast cells as a source of multifunctional cytokines. Immunol Today, 11, 458-464.
  • Kurtdede N, Yörük M (1995): Tavuk ve bıldırcın deri- sinde mast hücrelerinin morfolojik ve histometrik incelen- mesi. Ankara Üniv Vet Fak Derg, 42, 77-83.
  • Lehmann EL (1998): Non parametrics statistical methods based on ranks. First edition, Prentice-Hall, Inc. Simon&Schuster/ A Viacom Company Upper Saddle River, New Jersey.
  • Marshall JS, Bienenstock J (1994): The role of mast cells in inflammatory reactions of the airways, skin and intestine. Curr Opin Immunol, 6, 853-859.
  • Marshall JS, Ford GP, Bell EB (1987): Formalin sensitivity and differential staining of mast cells in human dermis. Br J Dermatol, 117, 29-36.
  • Mikhail GR, Miller-Milinska A (1964): Mast cell population in human skin. J Invest Dermatol, 43, 249-254.
  • Persinger MA, Lapage P, Simard JP, Barker GH (1983): Mast cell number in incisional wounds in rat skin as a function of distance, time and treatment. Br J Derma- tol, 108, 179-187.
  • Schwartz LB, Austen KF (1984): Structure and function of the chemical mediators of mast cells. Prog Allergy, 34, 271-321.
  • Scott DW, Miller WH, Griffin CE (1995): Muller and Kirk's small animal dermatology. 5th Edition,W. B. Saunders Company, Philadelphia, 488-500.
  • Shanahan F, Macniven I, Dyck N, Denburg JA, Bienenstock J, Befus AD (1987): Human lung mast cells: distribution and abundance of histochemicaly distinct subpopulations. lnt Arch Allergy Appl Immunol, 83, 329- 331.
  • Skaper SD, Pollock M, Facci L (2001): Mast cells differentially express and release active high molecular weight neurotrophins. Brain Res Mol Brain Res, 97, 177- 185.
  • Walton S, DeSouza EJ (1983): Variation in mast cell numbers in psoriasis and lichen planus: comparisons with normal skin. Dermatologica, 166, 236-239.
  • Warton A, Papadimitriou JM, Goldie RG, Paterson JW (1986): An ultrastructural study of mast cells in the alveolar wall of normal and asthmatic lung. Aust Exp Biol Med Sci, 64, 435-444. Geliş tarihi: 08.12.2003 / Kabul tarihi: 02.04.2004 Corresponding address: Prof. Dr. Reşat Nuri Aştı Ankara Üniversitesi Veteriner Fakültesi Histoloji-Embriyoloji Anabilim Dalı 06110 Dışkapı, Ankara e-mail: resatnuriasti@hotmail.com
There are 29 citations in total.

Details

Primary Language English
Subjects Veterinary Surgery
Journal Section Research Article
Authors

Reşat Nuri Aştı

Arif Kurtdede

Reşat Nuri Aştı

Emel Ergün

Murat Güzel

Publication Date March 1, 2005
Published in Issue Year 2005Volume: 52 Issue: 1

Cite

APA Aştı, R. N., Kurtdede, A., Aştı, R. N., Ergün, E., et al. (2005). Mast cells in the dog skin: distribution, density, heterogeneity and influence of fixation techniques. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 52(1), 7-12. https://doi.org/10.1501/Vetfak_0000000022
AMA Aştı RN, Kurtdede A, Aştı RN, Ergün E, Güzel M. Mast cells in the dog skin: distribution, density, heterogeneity and influence of fixation techniques. Ankara Univ Vet Fak Derg. March 2005;52(1):7-12. doi:10.1501/Vetfak_0000000022
Chicago Aştı, Reşat Nuri, Arif Kurtdede, Reşat Nuri Aştı, Emel Ergün, and Murat Güzel. “Mast Cells in the Dog Skin: Distribution, Density, Heterogeneity and Influence of Fixation Techniques”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 52, no. 1 (March 2005): 7-12. https://doi.org/10.1501/Vetfak_0000000022.
EndNote Aştı RN, Kurtdede A, Aştı RN, Ergün E, Güzel M (March 1, 2005) Mast cells in the dog skin: distribution, density, heterogeneity and influence of fixation techniques. Ankara Üniversitesi Veteriner Fakültesi Dergisi 52 1 7–12.
IEEE R. N. Aştı, A. Kurtdede, R. N. Aştı, E. Ergün, and M. Güzel, “Mast cells in the dog skin: distribution, density, heterogeneity and influence of fixation techniques”, Ankara Univ Vet Fak Derg, vol. 52, no. 1, pp. 7–12, 2005, doi: 10.1501/Vetfak_0000000022.
ISNAD Aştı, Reşat Nuri et al. “Mast Cells in the Dog Skin: Distribution, Density, Heterogeneity and Influence of Fixation Techniques”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 52/1 (March 2005), 7-12. https://doi.org/10.1501/Vetfak_0000000022.
JAMA Aştı RN, Kurtdede A, Aştı RN, Ergün E, Güzel M. Mast cells in the dog skin: distribution, density, heterogeneity and influence of fixation techniques. Ankara Univ Vet Fak Derg. 2005;52:7–12.
MLA Aştı, Reşat Nuri et al. “Mast Cells in the Dog Skin: Distribution, Density, Heterogeneity and Influence of Fixation Techniques”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 52, no. 1, 2005, pp. 7-12, doi:10.1501/Vetfak_0000000022.
Vancouver Aştı RN, Kurtdede A, Aştı RN, Ergün E, Güzel M. Mast cells in the dog skin: distribution, density, heterogeneity and influence of fixation techniques. Ankara Univ Vet Fak Derg. 2005;52(1):7-12.