Research Article
Histochemical and Immunohistochemical Investigations on Pyloric Tonsil in Turkeys (Meleagris gallopavo)
Abstract
Structural patterns of pyloric tonsil in turkeys (Meleagris gallopavo) were investigated in this study. Turkeys, less resistant to disease than chickens, are a crucial source of protein with low cholesterol levels and a high protein/calorie ratio. Pyloric tonsil, involved in the chicken defense barrier, is a component of GALT. To evaluate the specific region, adult turkeys were taken from slaughterhouses. Crossman's modified triple staining demonstrated the general histological structure, PAS revealed the density of goblet cells synthesizing mucus, and Safranin O showed the capsule around the follicles. The width of lymphoid follicles in the pyloric tonsil (204.0±22.33) differed significantly from that of the middle part of the duodenum (111.7±4.741). It was noticed that the specific area harbors T cells, B cells, and follicular dendritic cells. Interfollicular regions were infiltrated with CD3+T cells, but the CD268+B cells occupied primarily germinal centers. However, it was noticed that T cells were present in germinal centers and some B cells in the interfollicular region. Anti-Vimentin antibody revealed follicular dendritic cells in the pyloric zone. It was noted that the epithelial layer of the pyloric tonsil is devoid of CK18-positive cells. Findings of the semi-thin section indicated that some columnar epithelial cells were stained differently in the transition zone (lymphoepithelium). To sum up, a pyloric region in turkeys is structurally dissimilar to pyloric tonsils detected in chicken, chukar partridge, and duck. More research is needed to reveal the effect of pyloric tonsil on the alimentary tract in poultry in terms of defense mechanisms.
Keywords
Project Number
Grant number:17L0239011
References
- Referans1 Arugh PA, Hamedi S (2019): A histomorphometric study on age-related changes in selected lymphoid structures of Chukar partridge (Alectoris chukar). Iran J Vet Res, 20, 186-191.
- Referans2 Bancroft JD, Gamble M (2008): Theory and Practice of Histological Techniques. 6th edn. Churchill Livingstone, Elsevier, China.
- Referans3 Bucy RP, Chen CL, Cihak J, et al (1988): Avian T cells expressing γδ receptors localize in the splenic sinusoids and the intestinal epithelium. J Immunol, 141, 2200-2205.
- Referans4 Casteleyn C, Doom M, Lambrechts E, et al (2010): Locations of gut-associated lymphoid tissue in the 3-monthold chicken: a review. Avian Pathol, 39, 143–150.
- Referans5 Davison F, Kaspers B, Schat KA, et al (2011): Avian Immunology. 1st edn. MA: Elsevier/Academic Press, Boston.
- Referans6 Decaestecker E, Gaba S, Raeymaekers JA, et al (2007): Host-parasite 'Red Queen' dynamics archived in pond sediment. Nature, 450, 870-873.
- Referans7 Eurell JA, Frappier BL (2006): Textbook of Veterinary Histology, 6th edn. Blackwell Publishing Ltd, Oxford.
- Referans8 Fagerland JA, Arp LH (1993): Structure and development of bronchus-associated lymphoid tissue in conventionally reared broiler chickens. Avian Dis, 37, 10-18.
- Referans9 Gartner LP, Hiatt JL (1997): Digestive system II–Alimentary Canal. Color Textbook of Histology. 1st edn. PA: Saunders, Philadelphia.
- Referans10 Gedam PM, Nandeshwar NC, Salankar AM, et al (2018): Histomorphological and histochemical studies on lymphoid tissue of pyloric region in khaki campbell breed of duck (Anas platyrhynchos). J Entomol Zool Stud, 6, 1222-1224.
- Referans11 Guy-Grand D, Cerf-Bensussen N, Malissen B, et al (1991): Two gut intraepithelial CD8+ lymphocyte populations with different T cell receptors: a role for the gut epithelium in T cell differentiation. J Exp Med, 173, 471-481. Referans12 Hondo T, Kanaya T, Takakura I, et al (2011): Cytokeratin 18 is a specific marker of bovine intestinal M cell. Am J Physiol Gastrointest Liver Physiol, 300, G442-G453.
- Referans13 Kido T, Ohwada S, Watanabe K, et al (2003): Differential characteristics of microfold cells on the dome epithelium of porcine ileum. Anim Sci J, 74, 375-382.
- Referans14 Mason DY, Cordell J L, Brown MH, et al (1995): CD79a: a novel marker for B-cell neoplasms in routinely processed tissue samples. Blood, 86, 1453-1459.
- Referans15 Mörbe UM, Jørgensen PB, Fenton TM, et al (2021): Human gut-associated lymphoid tissues (GALT); diversity, structure, and function. Mucosal Immunol, 14, 793-802.
- Referans16 Nagy N, Oláh I (2007): Pyloric tonsil as a novel gut associated lymphoepithelial organ of the chicken. J Anat, 211, 407-411.
- Referans17 Oláh I, Nagy N, Magyar A, et al (2003): Esophageal tonsil: a novel gut associated lymphoid organ. Poultry Sci, 82, 767–770.
- Referans18 Oláh I, Nagy N, Vervelde L (2014): Structure of the avian lymphoid system. 11-44. In: Avian Immunology. F Davison (Ed), B Kaspers (Ed), KA Schat (Ed), P Kaiser (Ed). Elsevier, Philadelphia.
- Referans19 Perry ME (1994): The specialised structure of crypt epithelium in the human palatine tonsil and its functional significance. J Anat, 185, 111-127.
- Referans20 Reddy SK, Hu T, Gudivada R, et al (2008): The BAFF-Interacting receptors of chickens. Dev Comp Immunol, 32, 1076-1087.
- Referans21 Samuelson DA (2007): Textbook of Veterinary Histology. Saunders– Elsevier, St. Louis, Missouri.
- Referans22 Somayeh Hamedi DVM (2021): Histological characterization of the gut-associated lymphoid tissue in three-month old guinea fowls (Numida meleagris). Philipp J Vet Med, 58, 96-100.
- Referans23 van der Poel CE, Bajic G, Macaulay CW, et al (2019): Follicular dendritic cells modulate germinal center B cell diversity through FcγRIIB. Cell Rep, 29, 2745-2755.
- Referans24 Vervelde L, Jeurissen SHM (1993): Postnatal development of intra-epithelial leukocytes in the chicken digestive tract: phenotypical characterisation in situ. Cell Tissue Res, 274, 295-301.
- Referans25 Woolhouse ME, Webster JP, Domingo E, et al (2002): Biological and biomedical implications of the co-evolution of pathogens and their hosts. Nat Genet, 32, 569-577.
Year 2024,
Accepted Papers, 1 - 9
Abstract
Keywords
Supporting Institution
Ankara University Scientific Research Grant
Project Number
Grant number:17L0239011
Thanks
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References
- Referans1 Arugh PA, Hamedi S (2019): A histomorphometric study on age-related changes in selected lymphoid structures of Chukar partridge (Alectoris chukar). Iran J Vet Res, 20, 186-191.
- Referans2 Bancroft JD, Gamble M (2008): Theory and Practice of Histological Techniques. 6th edn. Churchill Livingstone, Elsevier, China.
- Referans3 Bucy RP, Chen CL, Cihak J, et al (1988): Avian T cells expressing γδ receptors localize in the splenic sinusoids and the intestinal epithelium. J Immunol, 141, 2200-2205.
- Referans4 Casteleyn C, Doom M, Lambrechts E, et al (2010): Locations of gut-associated lymphoid tissue in the 3-monthold chicken: a review. Avian Pathol, 39, 143–150.
- Referans5 Davison F, Kaspers B, Schat KA, et al (2011): Avian Immunology. 1st edn. MA: Elsevier/Academic Press, Boston.
- Referans6 Decaestecker E, Gaba S, Raeymaekers JA, et al (2007): Host-parasite 'Red Queen' dynamics archived in pond sediment. Nature, 450, 870-873.
- Referans7 Eurell JA, Frappier BL (2006): Textbook of Veterinary Histology, 6th edn. Blackwell Publishing Ltd, Oxford.
- Referans8 Fagerland JA, Arp LH (1993): Structure and development of bronchus-associated lymphoid tissue in conventionally reared broiler chickens. Avian Dis, 37, 10-18.
- Referans9 Gartner LP, Hiatt JL (1997): Digestive system II–Alimentary Canal. Color Textbook of Histology. 1st edn. PA: Saunders, Philadelphia.
- Referans10 Gedam PM, Nandeshwar NC, Salankar AM, et al (2018): Histomorphological and histochemical studies on lymphoid tissue of pyloric region in khaki campbell breed of duck (Anas platyrhynchos). J Entomol Zool Stud, 6, 1222-1224.
- Referans11 Guy-Grand D, Cerf-Bensussen N, Malissen B, et al (1991): Two gut intraepithelial CD8+ lymphocyte populations with different T cell receptors: a role for the gut epithelium in T cell differentiation. J Exp Med, 173, 471-481. Referans12 Hondo T, Kanaya T, Takakura I, et al (2011): Cytokeratin 18 is a specific marker of bovine intestinal M cell. Am J Physiol Gastrointest Liver Physiol, 300, G442-G453.
- Referans13 Kido T, Ohwada S, Watanabe K, et al (2003): Differential characteristics of microfold cells on the dome epithelium of porcine ileum. Anim Sci J, 74, 375-382.
- Referans14 Mason DY, Cordell J L, Brown MH, et al (1995): CD79a: a novel marker for B-cell neoplasms in routinely processed tissue samples. Blood, 86, 1453-1459.
- Referans15 Mörbe UM, Jørgensen PB, Fenton TM, et al (2021): Human gut-associated lymphoid tissues (GALT); diversity, structure, and function. Mucosal Immunol, 14, 793-802.
- Referans16 Nagy N, Oláh I (2007): Pyloric tonsil as a novel gut associated lymphoepithelial organ of the chicken. J Anat, 211, 407-411.
- Referans17 Oláh I, Nagy N, Magyar A, et al (2003): Esophageal tonsil: a novel gut associated lymphoid organ. Poultry Sci, 82, 767–770.
- Referans18 Oláh I, Nagy N, Vervelde L (2014): Structure of the avian lymphoid system. 11-44. In: Avian Immunology. F Davison (Ed), B Kaspers (Ed), KA Schat (Ed), P Kaiser (Ed). Elsevier, Philadelphia.
- Referans19 Perry ME (1994): The specialised structure of crypt epithelium in the human palatine tonsil and its functional significance. J Anat, 185, 111-127.
- Referans20 Reddy SK, Hu T, Gudivada R, et al (2008): The BAFF-Interacting receptors of chickens. Dev Comp Immunol, 32, 1076-1087.
- Referans21 Samuelson DA (2007): Textbook of Veterinary Histology. Saunders– Elsevier, St. Louis, Missouri.
- Referans22 Somayeh Hamedi DVM (2021): Histological characterization of the gut-associated lymphoid tissue in three-month old guinea fowls (Numida meleagris). Philipp J Vet Med, 58, 96-100.
- Referans23 van der Poel CE, Bajic G, Macaulay CW, et al (2019): Follicular dendritic cells modulate germinal center B cell diversity through FcγRIIB. Cell Rep, 29, 2745-2755.
- Referans24 Vervelde L, Jeurissen SHM (1993): Postnatal development of intra-epithelial leukocytes in the chicken digestive tract: phenotypical characterisation in situ. Cell Tissue Res, 274, 295-301.
- Referans25 Woolhouse ME, Webster JP, Domingo E, et al (2002): Biological and biomedical implications of the co-evolution of pathogens and their hosts. Nat Genet, 32, 569-577.
There are 24 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery |
| Journal Section | Research Article |
| Authors | |
| Project Number | Grant number:17L0239011 |
| Early Pub Date | September 21, 2023 |
| Publication Date | |
| Published in Issue | Year 2024Accepted Papers |