Research Article
Year 2024,
Accepted Papers, 1 - 13
Abstract
Project Number
21L0239012
References
- Akdeşir E, Özyiğit MÖ, Kahraman MM (2018): Sığırlarda Mycobacterium bovis’in moleküler ve sito-histopatolojik tanı yöntemleri ile gösterilmesi ve sonuçlarının karşılaştırılması. Ankara Univ Vet Fak Derg, 66, 27-35.
- Aranaz A, Lıébana E, Gómez-Mampaso E, et al (1999): Mycobacterium tuberculosis subsp. caprae subsp. nov.: a taxonomic study of a new member of the Mycobacterium tuberculosis complex isolated from goats in Spain. Int J Syst Bacteriol, 49, 1263-1273.
- Aslan G, Kuyucu N, Çalıkoğlu M, et al (2009): Mycobacterium bovis’in etken olduğu tüberküloz olguları. ANKEM Derg, 23, 182-187.
- Avsever ML, Çavuşoğlu C, Yazıcıoğlu Ö, et al (2017): New spoligotyping pattern of Mycobacterium bovis isolates from farm animals in Turkey. Ankara Univ Vet Fak Derg, 64, 37-43.
- Avsever M, Çavuşoğlu C, Çamkerten I (2020): First isolation of Mycobacterium bovis SIT 482 BOV from beef cattle in Turkey. Journal of the Hellenic Veterinary Medical Society, 71(3), 2279–2282.
- Belakehal F, Barth SA, Menge C, et al (2022): Evaluation of the discriminatory power of spoligotyping and 19-locus mycobacterial interspersed repetitive unit-variable number of tandem repeat analysis (MIRU-VNTR) of Mycobacterium bovis strains isolated from cattle in Algeria. PLoS One, 17, e0262390.
- Bolado- Martínez E, Benavides-Dávila I, Candia-Plata Mdel C, et al (2015): Proposal of a screening MIRU-VNTR panel for the preliminary genotyping of Mycobacterium bovis in Mexico. Biomed Res Int, 2015, 416479.
- Cadmus S, Akinseye VO, van Soolingen D (2019): Mycobacterium bovis in humans and M. tuberculosis in animals in Nigeria: an overview from 1975-2014. Int J Tuberc Lung Dis, 23, 1162-1170.
- Cinoğlu H (2021): Adıyaman ilinde tüberküloz şüphesi olan sığırlarda Mycobacterium bovis varlığının araştırılması ve epidemiyolojik tiplendirilmesi. Yüksek Lisans Tezi.
- Collins DM (2011): Advances in molecular diagnostics for Mycobacterium bovis. Vet. Microbiol. 151, 2–7.
- Çavuşoğlu C, Yılmaz FF (2017): Ege Bölgesi’nde insan Mycobacterium bovis enfeksiyonlarının moleküler epidemiyolojisi. Mikrobiyoloji Bülteni, 51, 165-170.
- Dibaba AB, Kriek NPJ, Thoen CO (2019): Tuberculosis in Animals: An African Perspective. Cham: Springer.
- Gagneux S (2012): Host-pathogen coevolution in human tuberculosis. Philos Trans R Soc Lond B Biol Sci, 367, 850-859.
- Gülcü Y, Hadımlı HH (2020): Typing of Mycobacterium bovis isolates from cattle using MIRU-VNTR analysis. Etlik Veteriner Mikrobiyoloji Dergisi, 31, 133-139.
- Kremer K, Glynn JR, Lillebaek T, et al (2004): Definition of the Beijing/W lineage of Mycobacterium tuberculosis on the basis of genetic markers. J Clin Microbiol, 42, 4040-4049.
- Melo EH, Gomes HM, Suffys PN, et al (2021): Genotypic characterization of Mycobacterium bovis Isolates from dairy cattle diagnosed with clinical tuberculosis. Front Vet Sci, 8, 747226.
- OIE WOAH (2023): Bovine tuberculosis. Available at https://www.oie.int/en/what-we-do/animal-health-and-welfare/animal-diseases/old-classification-of-diseases-notifiable-to-the-oie-list-b (Accessed October 5, 2023).
- Oruene IS, Ndukwe SC (2020): An assessment of meat inspection for bovine tuberculosis and the functional conditions of major abattoirs/ slaughter slabs in rivers state. Black Sea Journal of Agriculture, 3, 205-210.
- Parreiras PM, Andrade GI, Nascimento Tde F, et al (2012): Spoligotyping and variable number tandem repeat analysis of Mycobacterium bovis isolates from cattle in Brazil. Mem Inst Oswaldo Cruz, 107, 64-73.
- Prodinger WM, Indra A, Koksalan OK, et al (2014): Mycobacterium caprae infection in humans. Expert Rev Anti Infect Ther, 12, 1501-1513.
- Sahraoui N, Müller B, Guetarni D, et al (2009): Molecular characterization of Mycobacterium bovis strains isolated from cattle slaughtered at two abattoirs in Algeria. BMC Vet Res, 5, 4.
- Sayın Z, Erganiş O (2011): Identification of Mycobacterium strains by PCR and PCR-REA. Bull Vet Inst Pulawy, 55, 641-644.
- Supply P, Allix C, Lesjean S, et al (2006): Proposal for standardization of optimized mycobacterial interspersed repetitive unit-variable-number tandem repeat typing of Mycobacterium tuberculosis. J Clin Microbiol, 44, 4498–4510.
- Thoen CO, Steele JH, Gilsdorf MJ (2006): Mycobacterium bovis infection in animals and humans. 2nd Edition. Ames, Iowa: Wiley-Blackwell; London: Eurospan.
- Thoen CO, Steele JH, Kaneene JB (2014): Zoonotic tuberculosis: Mycobacterium bovis and other pathogenic mycobacteria. 3rd Edition. Ames, Iowa: Wiley-Blackwell.
- Tuzcu N, Köksal F (2020): Genetic evaluation of Mycobacterium bovis isolates with MIRU-VNTR and spoligotyping. Turkish Journal of Medical Sciences, 50, 2017-2023.
- UTTR (2014): Türkiye Halk Sağlığı Kurumu, Sağlık Bakanlığı Yayın No: 935, Ankara.
- Wei CY, Hsu YH, Chou WJ, et al (2004): Molecular and histopathologic evidence for systetmic infection by Mycobacterium bovis in a patient with tuberculous enteritis, peritonitis, and meningitis: a case report. The Kaohsiung Journal of Medical Sciences, 20, 302–307.
- WHO Global TB Report (2020): Geneva, Switzerland. Available at https://www.who.int/publications/i/item/9789240013131 (Accessed October 5, 2023).
- Yardımcı H, Ünal CB, Ataseven LK, et al (2007): Sığır tüberkülozunun PCR ile tanısı ve Mycobacterium bovis’in spoligotiplendirme yöntemi ile genotiplendirilmesi. Ankara Univ Vet Fak Derg, 54, 183-189.
Epidemiological Analysis of Human and Animal Originated Mycobacterium bovis Strains by Spoligotyping and MIRU-VNTR Methods
Abstract
This study aims to investigate the genotypic similarities between human and animal-originated isolates by spoligotyping and 24 loci MIRU-VNTR for molecular epidemiological analysis of M. bovis isolates. In this study, isolates were obtained between 2019-2022 from 58 humans and 50 bovines. All isolates were initially identified with the GenoType MTBC kit and genotyped with spoligotyping and 24 loci MIRU-VNTR methods and their lineage relationships were shown in the dendrogram. When the human and animal-originated isolates were tested by the spoligotyping method, eight different clusters and 29 different genotypes were observed. Among these genotypes, the most common ones were found to be SIT1118/SB0989 (19.23%), SIT482/SB0120 (16.35%), SIT685/SB0288 (12.5%) and they were detected in isolates of both human and animal-originated. SB1593 (12.5%) was detected only in isolates of animal-originated. Other genotypes were found as SIT3529/SB0920, SIT1185/SB0897, SIT3710/SB1595, SIT688/SB0129, SIT3687/SB1625, SB0419, SB2466, SB1231, and SB2510. Nine different clusters and 55 different genotypes were obtained with MIRU-VNTR. ETR-C, QUB2163b, QUB26, and Mtub04 had the most allelic diversity. It was observed that MIRU02, MIRU20, MIRU24, MIRU27, and MIRU39 did not indicate allelic diversity. In conclusion, it was seen that the spoligotyping was easier to implement and evaluate compared to MIRU-VNTR. It was concluded that both tests can be used safely. Similar genotypes in humans and animals indicate the importance of zoonotic transmission of bovine tuberculosis.
Ethical Statement
Etik Kurul onayı gerekli değildir. TİTCK izni gerekli değildir.
Supporting Institution
Ankara Üniversitesi BAP Koordinatörlüğü
Project Number
21L0239012
Thanks
This study was derived from the PhD thesis of the first author. We would like to thank Prof. Dr. Mehmet AKAN (Department of Veterinary Microbiology, Ankara University) and Doç. Dr. Yasemin COŞGUN (National Virology Reference Laboratory) for their valuable contributions to the study.
References
- Akdeşir E, Özyiğit MÖ, Kahraman MM (2018): Sığırlarda Mycobacterium bovis’in moleküler ve sito-histopatolojik tanı yöntemleri ile gösterilmesi ve sonuçlarının karşılaştırılması. Ankara Univ Vet Fak Derg, 66, 27-35.
- Aranaz A, Lıébana E, Gómez-Mampaso E, et al (1999): Mycobacterium tuberculosis subsp. caprae subsp. nov.: a taxonomic study of a new member of the Mycobacterium tuberculosis complex isolated from goats in Spain. Int J Syst Bacteriol, 49, 1263-1273.
- Aslan G, Kuyucu N, Çalıkoğlu M, et al (2009): Mycobacterium bovis’in etken olduğu tüberküloz olguları. ANKEM Derg, 23, 182-187.
- Avsever ML, Çavuşoğlu C, Yazıcıoğlu Ö, et al (2017): New spoligotyping pattern of Mycobacterium bovis isolates from farm animals in Turkey. Ankara Univ Vet Fak Derg, 64, 37-43.
- Avsever M, Çavuşoğlu C, Çamkerten I (2020): First isolation of Mycobacterium bovis SIT 482 BOV from beef cattle in Turkey. Journal of the Hellenic Veterinary Medical Society, 71(3), 2279–2282.
- Belakehal F, Barth SA, Menge C, et al (2022): Evaluation of the discriminatory power of spoligotyping and 19-locus mycobacterial interspersed repetitive unit-variable number of tandem repeat analysis (MIRU-VNTR) of Mycobacterium bovis strains isolated from cattle in Algeria. PLoS One, 17, e0262390.
- Bolado- Martínez E, Benavides-Dávila I, Candia-Plata Mdel C, et al (2015): Proposal of a screening MIRU-VNTR panel for the preliminary genotyping of Mycobacterium bovis in Mexico. Biomed Res Int, 2015, 416479.
- Cadmus S, Akinseye VO, van Soolingen D (2019): Mycobacterium bovis in humans and M. tuberculosis in animals in Nigeria: an overview from 1975-2014. Int J Tuberc Lung Dis, 23, 1162-1170.
- Cinoğlu H (2021): Adıyaman ilinde tüberküloz şüphesi olan sığırlarda Mycobacterium bovis varlığının araştırılması ve epidemiyolojik tiplendirilmesi. Yüksek Lisans Tezi.
- Collins DM (2011): Advances in molecular diagnostics for Mycobacterium bovis. Vet. Microbiol. 151, 2–7.
- Çavuşoğlu C, Yılmaz FF (2017): Ege Bölgesi’nde insan Mycobacterium bovis enfeksiyonlarının moleküler epidemiyolojisi. Mikrobiyoloji Bülteni, 51, 165-170.
- Dibaba AB, Kriek NPJ, Thoen CO (2019): Tuberculosis in Animals: An African Perspective. Cham: Springer.
- Gagneux S (2012): Host-pathogen coevolution in human tuberculosis. Philos Trans R Soc Lond B Biol Sci, 367, 850-859.
- Gülcü Y, Hadımlı HH (2020): Typing of Mycobacterium bovis isolates from cattle using MIRU-VNTR analysis. Etlik Veteriner Mikrobiyoloji Dergisi, 31, 133-139.
- Kremer K, Glynn JR, Lillebaek T, et al (2004): Definition of the Beijing/W lineage of Mycobacterium tuberculosis on the basis of genetic markers. J Clin Microbiol, 42, 4040-4049.
- Melo EH, Gomes HM, Suffys PN, et al (2021): Genotypic characterization of Mycobacterium bovis Isolates from dairy cattle diagnosed with clinical tuberculosis. Front Vet Sci, 8, 747226.
- OIE WOAH (2023): Bovine tuberculosis. Available at https://www.oie.int/en/what-we-do/animal-health-and-welfare/animal-diseases/old-classification-of-diseases-notifiable-to-the-oie-list-b (Accessed October 5, 2023).
- Oruene IS, Ndukwe SC (2020): An assessment of meat inspection for bovine tuberculosis and the functional conditions of major abattoirs/ slaughter slabs in rivers state. Black Sea Journal of Agriculture, 3, 205-210.
- Parreiras PM, Andrade GI, Nascimento Tde F, et al (2012): Spoligotyping and variable number tandem repeat analysis of Mycobacterium bovis isolates from cattle in Brazil. Mem Inst Oswaldo Cruz, 107, 64-73.
- Prodinger WM, Indra A, Koksalan OK, et al (2014): Mycobacterium caprae infection in humans. Expert Rev Anti Infect Ther, 12, 1501-1513.
- Sahraoui N, Müller B, Guetarni D, et al (2009): Molecular characterization of Mycobacterium bovis strains isolated from cattle slaughtered at two abattoirs in Algeria. BMC Vet Res, 5, 4.
- Sayın Z, Erganiş O (2011): Identification of Mycobacterium strains by PCR and PCR-REA. Bull Vet Inst Pulawy, 55, 641-644.
- Supply P, Allix C, Lesjean S, et al (2006): Proposal for standardization of optimized mycobacterial interspersed repetitive unit-variable-number tandem repeat typing of Mycobacterium tuberculosis. J Clin Microbiol, 44, 4498–4510.
- Thoen CO, Steele JH, Gilsdorf MJ (2006): Mycobacterium bovis infection in animals and humans. 2nd Edition. Ames, Iowa: Wiley-Blackwell; London: Eurospan.
- Thoen CO, Steele JH, Kaneene JB (2014): Zoonotic tuberculosis: Mycobacterium bovis and other pathogenic mycobacteria. 3rd Edition. Ames, Iowa: Wiley-Blackwell.
- Tuzcu N, Köksal F (2020): Genetic evaluation of Mycobacterium bovis isolates with MIRU-VNTR and spoligotyping. Turkish Journal of Medical Sciences, 50, 2017-2023.
- UTTR (2014): Türkiye Halk Sağlığı Kurumu, Sağlık Bakanlığı Yayın No: 935, Ankara.
- Wei CY, Hsu YH, Chou WJ, et al (2004): Molecular and histopathologic evidence for systetmic infection by Mycobacterium bovis in a patient with tuberculous enteritis, peritonitis, and meningitis: a case report. The Kaohsiung Journal of Medical Sciences, 20, 302–307.
- WHO Global TB Report (2020): Geneva, Switzerland. Available at https://www.who.int/publications/i/item/9789240013131 (Accessed October 5, 2023).
- Yardımcı H, Ünal CB, Ataseven LK, et al (2007): Sığır tüberkülozunun PCR ile tanısı ve Mycobacterium bovis’in spoligotiplendirme yöntemi ile genotiplendirilmesi. Ankara Univ Vet Fak Derg, 54, 183-189.
There are 30 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Bacteriology, Veterinary Epidemiology, Veterinary Microbiology |
| Journal Section | Research Article |
| Authors | |
| Project Number | 21L0239012 |
| Early Pub Date | June 28, 2024 |
| Publication Date | |
| Submission Date | October 13, 2023 |
| Acceptance Date | March 11, 2024 |
| Published in Issue | Year 2024Accepted Papers |