The eftects of dinerent N-nitroso compounds in primary cultures of human and rat hepatocytes on the DNA

Taner Pamukçu; Alexandre Scott

doi:10.1501/Vetfak_0000001641

Letter to Editor

İnsan ve rat karaciğer primer hücre kültürlerinde farklı N-nitroso bileşiklerinin DNA üzerindeki etkileri

Year 2002, Volume: 49 Issue: 1, 23 - 29, 01.03.2002

Taner Pamukçu Alexandre Scott

https://doi.org/10.1501/Vetfak_0000001641

Abstract

References

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The eftects of dinerent N-nitroso compounds in primary cultures of human and rat hepatocytes on the DNA

Year 2002, Volume: 49 Issue: 1, 23 - 29, 01.03.2002

Taner Pamukçu Alexandre Scott

https://doi.org/10.1501/Vetfak_0000001641

Abstract

Ten earcinogenic N-nitroso eompounds were examined for DNA-damaging activity in prımary cultures of human
'1nd rat h,:patocytes. DNA fragmentation was mcasured hy the alkaline dution techniClue. and unscheduled Df\A synthesis was by
qu,ıntitatiıe mıtoradiography. Positive dose-related responses in the range of subtoxic eoncentrations indicated were obtained ın cclls
of hoth species with N.nitrosodiethylanııne (I 0-32 mM). :"J-nitrosodi-n-propylamine (ı .s-l O mM). N-nitrosomorpholine (i -3.2 mM).
N nıtroscpıperidine (1-3.2 mM). N-nitrosopyrrolidine (3.2-1 S mM). N-nitroso-N-methylurca (O.32-I.S mM). N-nitroso-i\ .ethylurea
(0.32-1.8 mM) and N-nitroso-f\hutylurea (O. i-0.32 mM). N-nitrosodi-n-hutylamine was practicaııy inactive at the maximal soluble
conccntr,.tion (I mM). The results obtained from human hepatoeytes were Clualitatively similar LO those of rat hepatoeytes. hut
staıistieaııy. imp0rlant differenees hetween the two speeies in the amounts of DNA damage and/or unseheduled DNA synthesis were
observed wiıh N-nitrosodimethylamine. f\-nitrosomorpholine, N-nitrosopiperidine. N-nitrosopyrrolidine and N-nitroso-f\.
huıylıırca. On the other hand, 4uantitative differences in the gcnotoxie effeets indueed hy 5 mM-N-nitrosüdimethylaınine in eultures
derived from 20 human donors and from 20 rat were greater than average eoınpounds. These results indieate thaı the rat hepatocyte
DNA repair assay is a valid lI1terspeeies diflen::nccs displayed hy this nitrosamine and hy other N-niıroso model for predicting the
genotoxi" potentıal of N-nitroso compounds in human hepatocytes.

Keywords

DNA damage, human, rat

References

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There are 1 citations in total.

Details

Primary Language	English
Subjects	Veterinary Surgery
Journal Section	Research Article
Authors	Taner Pamukçu Alexandre Scott
Publication Date	March 1, 2002
Published in Issue	Year 2002Volume: 49 Issue: 1

Cite

APA	Pamukçu, T., & Scott, A. (2002). The eftects of dinerent N-nitroso compounds in primary cultures of human and rat hepatocytes on the DNA. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 49(1), 23-29. https://doi.org/10.1501/Vetfak_0000001641
AMA	Pamukçu T, Scott A. The eftects of dinerent N-nitroso compounds in primary cultures of human and rat hepatocytes on the DNA. Ankara Univ Vet Fak Derg. March 2002;49(1):23-29. doi:10.1501/Vetfak_0000001641
Chicago	Pamukçu, Taner, and Alexandre Scott. “The Eftects of Dinerent N-Nitroso Compounds in Primary Cultures of Human and Rat Hepatocytes on the DNA”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 49, no. 1 (March 2002): 23-29. https://doi.org/10.1501/Vetfak_0000001641.
EndNote	Pamukçu T, Scott A (March 1, 2002) The eftects of dinerent N-nitroso compounds in primary cultures of human and rat hepatocytes on the DNA. Ankara Üniversitesi Veteriner Fakültesi Dergisi 49 1 23–29.
IEEE	T. Pamukçu and A. Scott, “The eftects of dinerent N-nitroso compounds in primary cultures of human and rat hepatocytes on the DNA”, Ankara Univ Vet Fak Derg, vol. 49, no. 1, pp. 23–29, 2002, doi: 10.1501/Vetfak_0000001641.
ISNAD	Pamukçu, Taner - Scott, Alexandre. “The Eftects of Dinerent N-Nitroso Compounds in Primary Cultures of Human and Rat Hepatocytes on the DNA”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 49/1 (March 2002), 23-29. https://doi.org/10.1501/Vetfak_0000001641.
JAMA	Pamukçu T, Scott A. The eftects of dinerent N-nitroso compounds in primary cultures of human and rat hepatocytes on the DNA. Ankara Univ Vet Fak Derg. 2002;49:23–29.
MLA	Pamukçu, Taner and Alexandre Scott. “The Eftects of Dinerent N-Nitroso Compounds in Primary Cultures of Human and Rat Hepatocytes on the DNA”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 49, no. 1, 2002, pp. 23-29, doi:10.1501/Vetfak_0000001641.
Vancouver	Pamukçu T, Scott A. The eftects of dinerent N-nitroso compounds in primary cultures of human and rat hepatocytes on the DNA. Ankara Univ Vet Fak Derg. 2002;49(1):23-9.