Ten earcinogenic N-nitroso eompounds were examined for DNA-damaging activity in prımary cultures of human '1nd rat h,:patocytes. DNA fragmentation was mcasured hy the alkaline dution techniClue. and unscheduled Df\A synthesis was by qu,ıntitatiıe mıtoradiography. Positive dose-related responses in the range of subtoxic eoncentrations indicated were obtained ın cclls of hoth species with N.nitrosodiethylanııne (I 0-32 mM). :"J-nitrosodi-n-propylamine (ı .s-l O mM). N-nitrosomorpholine (i -3.2 mM). N nıtroscpıperidine (1-3.2 mM). N-nitrosopyrrolidine (3.2-1 S mM). N-nitroso-N-methylurca (O.32-I.S mM). N-nitroso-i\ .ethylurea (0.32-1.8 mM) and N-nitroso-f\hutylurea (O. i-0.32 mM). N-nitrosodi-n-hutylamine was practicaııy inactive at the maximal soluble conccntr,.tion (I mM). The results obtained from human hepatoeytes were Clualitatively similar LO those of rat hepatoeytes. hut staıistieaııy. imp0rlant differenees hetween the two speeies in the amounts of DNA damage and/or unseheduled DNA synthesis were observed wiıh N-nitrosodimethylamine. f\-nitrosomorpholine, N-nitrosopiperidine. N-nitrosopyrrolidine and N-nitroso-f\. huıylıırca. On the other hand, 4uantitative differences in the gcnotoxie effeets indueed hy 5 mM-N-nitrosüdimethylaınine in eultures derived from 20 human donors and from 20 rat were greater than average eoınpounds. These results indieate thaı the rat hepatocyte DNA repair assay is a valid lI1terspeeies diflen::nccs displayed hy this nitrosamine and hy other N-niıroso model for predicting the genotoxi" potentıal of N-nitroso compounds in human hepatocytes.
Pamukçu, T., & Scott, A. (2002). The eftects of dinerent N-nitroso compounds in primary cultures of human and rat hepatocytes on the DNA. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 49(1), 23-29. https://doi.org/10.1501/Vetfak_0000001641
AMA
Pamukçu T, Scott A. The eftects of dinerent N-nitroso compounds in primary cultures of human and rat hepatocytes on the DNA. Ankara Univ Vet Fak Derg. March 2002;49(1):23-29. doi:10.1501/Vetfak_0000001641
Chicago
Pamukçu, Taner, and Alexandre Scott. “The Eftects of Dinerent N-Nitroso Compounds in Primary Cultures of Human and Rat Hepatocytes on the DNA”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 49, no. 1 (March 2002): 23-29. https://doi.org/10.1501/Vetfak_0000001641.
EndNote
Pamukçu T, Scott A (March 1, 2002) The eftects of dinerent N-nitroso compounds in primary cultures of human and rat hepatocytes on the DNA. Ankara Üniversitesi Veteriner Fakültesi Dergisi 49 1 23–29.
IEEE
T. Pamukçu and A. Scott, “The eftects of dinerent N-nitroso compounds in primary cultures of human and rat hepatocytes on the DNA”, Ankara Univ Vet Fak Derg, vol. 49, no. 1, pp. 23–29, 2002, doi: 10.1501/Vetfak_0000001641.
ISNAD
Pamukçu, Taner - Scott, Alexandre. “The Eftects of Dinerent N-Nitroso Compounds in Primary Cultures of Human and Rat Hepatocytes on the DNA”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 49/1 (March 2002), 23-29. https://doi.org/10.1501/Vetfak_0000001641.
JAMA
Pamukçu T, Scott A. The eftects of dinerent N-nitroso compounds in primary cultures of human and rat hepatocytes on the DNA. Ankara Univ Vet Fak Derg. 2002;49:23–29.
MLA
Pamukçu, Taner and Alexandre Scott. “The Eftects of Dinerent N-Nitroso Compounds in Primary Cultures of Human and Rat Hepatocytes on the DNA”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 49, no. 1, 2002, pp. 23-29, doi:10.1501/Vetfak_0000001641.
Vancouver
Pamukçu T, Scott A. The eftects of dinerent N-nitroso compounds in primary cultures of human and rat hepatocytes on the DNA. Ankara Univ Vet Fak Derg. 2002;49(1):23-9.
