Research Article
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Year 2019, Volume: 66 Issue: 1, 7 - 11, 31.12.2018

Gizem Çufaoğlu Aşkın Nur Derinöz Naim Deniz Ayaz

Abstract

References

  • 1. Abuladze T, Li M, Menetrez MY, et al. (2008): Bacteriophages reduce experimental contamination of hard surfaces, tomato, spinach, broccoli, and ground beef by Escherichia coli O157:H7. Appl Environ Microbiol, 74, 6230-6238.
  • 2. Aksu H, Özgen Arun Ö, Aydın A, et al (1999): Eschericiha coli O157:H7’nin hayvansal kökenli çeşitli gıda maddelerinde varlığı. Pendik Vet Mikrobiyol Derg, 30, 77-81.
  • 3. Anany H, Chen W, Pelton R, et al. (2011): Biocontrol of Listeria monocytogenes and Escherichia coli O157:H7 in meat by using phages immobilized on modified cellulose membranes. Appl Environ Microbiol, 77, 6379-6387.
  • 4. Arslan A (2013): Et muayenesi ve Et Ürünleri Teknolojisi. Medipres, Malatya.
  • 5. Ayaz ND, Cufaoglu G, Ormeci E, et al. (2016): Presence of Staphylococcus aureus and Shiga toxigenic Escherichia coli O157:H7 in Raw Meat in Ağrı, Turkey. Int J Enteric Pathog, 4, e36523.
  • 6. Ayaz ND, Gencay YE, Erol I (2014): Prevalence and molecular characterization of sorbitol fermenting and nonfermenting Escherichia coli O157: H7+/H7–isolated from cattle at slaughterhouse and slaughterhouse wastewater. Int J Food Microbiol, 174, 31-38.
  • 7. Balpetek D, Gürbüz Ü (2010): Investigations on the presence of E. coli O157: H7 in some meat products. Eurasian J Vet Sci, 26, 25-31.
  • 8. Bruttin A, Brüssow H (2005): Human volunteers receiving Escherichia coli phage T4 orally: a safety test of phage therapy. Antimicrob Agents Chemother, 49, 2874-2878.
  • 9. Brüssow H, Kutter E (2005): Phage ecology. 129-163. In: E Kutter, A Sulakvelizde (Eds), Bacteriophages: biology and applications. CRC Press, Washington DC.
  • 10. Büyükünal SK, Şakar FŞ, Turhan I, et al. (2016): Presence of Salmonella spp., Listeria monocytogenes, Escherichia coli 0157 and Nitrate-Nitrite Residue Levels in Turkish Traditional Fermented Meat Products (Sucuk and Pastirma). Kafkas Univ Vet Fak Derg, 22, 233-236.
  • 11. Chan BK, Abedon ST, Loc-Carrillo C (2013): Phage cocktails and the future of phage therapy. Future Microbiol, 8, 769-783.
  • 12. Erol İ (2007): Gıda Hijyeni ve Mikrobiyolojisi. Pozitif Matbaacılık, Ankara.
  • 13. Erol I, Goncuoglu M, Ayaz ND et al. (2016): Comparison of prevalence and genetic diversity of Escherichia coli O157:H7 in cattle and sheep. J Microbiol Biotechnol Food Sci, 6, 808-812.
  • 14. Gencay YE, Ayaz ND, Copuroglu G, et al. (2016): Biocontrol of Shiga Toxigenic Escherichia coliO157:H7 in Turkish Raw Meatball by Bacteriophage. J Food Saf, 36, 120-131.
  • 15. Guenther S, Huwyler D, Richard S, et al. (2009): Virulent bacteriophage for efficient biocontrol of Listeria monocytogenes in ready-to-eat foods. Appl Environ Microbiol, 75, 93-100.
  • 16. Hudson JA, Billington C, Cornelius A, et al. (2013): Use of a bacteriophage to inactivate Escherichia coli O157: H7 on beef. Food Microbiol, 36, 14-21.
  • 17. Kazi M, Annapure US. (2016): Bacteriophage biocontrol of foodborne pathogens. J Food Sci Technol, 53, 1355- 1362.
  • 18. Kudva IT, Jelacic S, Tarr PI, et al. (1999): Biocontrol of Escherichia coli O157 with O157-specific bacteriophages. Appl Environ Microbiol, 65, 3767-3773.
  • 19. Leverentz B, Conway WS, Camp MJ, et al. (2003): Biocontrol of Listeria monocytogenes on fresh-cut produce by treatment with lytic bacteriophages and a bacteriocin. Appl Environ Microbiol, 69, 4519-4526.
  • 20. Meng J, Doyle M, Zhao T, et al. (2001): Enterohemorrhagic Escherichia coli O157:H7. 193-213. In: MP Doyle, LR Beuchat, TJ Montville (Eds), Food Microbiology: Fundamentals and Frontiers. ASM press, Washington DC.
  • 21. O'Flaherty S, Ross RP, Coffey A (2009): Bacteriophage and their lysins for elimination of infectious bacteria. FEMS Microbiol Rev, 33, 801-819.
  • 22. O'Flynn G, Ross RP, Fitzgerald GF, et al. (2004): Evaluation of a cocktail of three bacteriophages for biocontrol of Escherichia coli O157:H7. Appl Environ Microbiol, 70, 3417-3424.
  • 23. Rozema EA, Stephens TP, Bach SJ, et al. (2009): Oral and rectal administration of bacteriophages for control of Escherichia coli O157: H7 in feedlot cattle. J Food Prot, 72, 241-250.
  • 24. Schmidt H, Beutin L, Karch H (1995): Molecular analysis of the plasmid-encoded hemolysin of Escherichia coli O157: H7 strain EDL 933. Infect immun, 63, 1055-1061.
  • 25. Sharma M, Patel JR, Conway WS, et al. (2009): Effectiveness of bacteriophages in reducing Escherichia coli O157: H7 on fresh-cut cantaloupes and lettuce. J Food Prot, 72, 1481-1485.
  • 26. Tanji Y, Shimada T, Yoichi M, et al. (2004): Toward rational control of Escherichia coli O157: H7 by a phage cocktail. Appl Microbiol Tech, 64, 270-274.
  • 27. Turkish Food Codex Legislation (2000): Et Ürünleri Tebliği. Resmi Gazete, 23960.
  • 28. US Food and Administration (2011): Inventory of effective food contact substance (FCS) notifications. FCN no. 1018. 2011.
  • 29. Viazis S, Akhtar M, Feirtag J, et al. (2011): Reduction of Escherichia coli O157: H7 viability on leafy green vegetables by treatment with a bacteriophage mixture and trans-cinnamaldehyde. Food Microbiol, 28,149-157.
  • 30. Viscardi M, Perugini AG, Auriemma C, et al. (2008): Isolation and characterisation of two novel coliphages with high potential to control antibiotic-resistant pathogenic Escherichia coli (EHEC and EPEC). Int J Antimicrob Agents, 31, 152-157.

An investigation on biocontrol of Escherichia coli O157:H7 by a bacteriophage cocktail in pastirma

Year 2019, Volume: 66 Issue: 1, 7 - 11, 31.12.2018

Gizem Çufaoğlu Aşkın Nur Derinöz Naim Deniz Ayaz

Abstract

In this study, it was aimed to investigate the biocontrol of Escherichia coli O157:H7 in pastirma (a traditional
Turkish meat product produced and consumed without heat treatment) by bacteriophages. A cocktail was prepared from two precharacterized phages which were isolated from cattle slaughterhouse wastewaters and were found to have lytic activity against E. coli
O157:H7. The phage cocktail at a concentration of 109 pfu/ml was applied to pastirma slices which experimentally contaminated at 6,9
x 103 cfu/ml and incubated for one week at room temperature (22-24ºC). During the incubation period, reduction effects of
bacteriophages on E. coli O157:H7 were investigated on certain times and days. As a result, the phage cocktail was able to reduce the
bacterial count approximately 2 log cfu/g in the bacteriophage applied group, therefore the number of E. coli O157:H7 decreased and
remained below the detection limit (< 10 cfu/g) during the experiment. The bacteriophage cocktail consisting of phage M8AEC16 and
phage M12BEC16 has been found to be effectively usable for biocontrol of E. coli O157:H7 in pastirma.  

Keywords

Bacteriophage biocontrol E. coli O157:H7 pastirma

References

  • 1. Abuladze T, Li M, Menetrez MY, et al. (2008): Bacteriophages reduce experimental contamination of hard surfaces, tomato, spinach, broccoli, and ground beef by Escherichia coli O157:H7. Appl Environ Microbiol, 74, 6230-6238.
  • 2. Aksu H, Özgen Arun Ö, Aydın A, et al (1999): Eschericiha coli O157:H7’nin hayvansal kökenli çeşitli gıda maddelerinde varlığı. Pendik Vet Mikrobiyol Derg, 30, 77-81.
  • 3. Anany H, Chen W, Pelton R, et al. (2011): Biocontrol of Listeria monocytogenes and Escherichia coli O157:H7 in meat by using phages immobilized on modified cellulose membranes. Appl Environ Microbiol, 77, 6379-6387.
  • 4. Arslan A (2013): Et muayenesi ve Et Ürünleri Teknolojisi. Medipres, Malatya.
  • 5. Ayaz ND, Cufaoglu G, Ormeci E, et al. (2016): Presence of Staphylococcus aureus and Shiga toxigenic Escherichia coli O157:H7 in Raw Meat in Ağrı, Turkey. Int J Enteric Pathog, 4, e36523.
  • 6. Ayaz ND, Gencay YE, Erol I (2014): Prevalence and molecular characterization of sorbitol fermenting and nonfermenting Escherichia coli O157: H7+/H7–isolated from cattle at slaughterhouse and slaughterhouse wastewater. Int J Food Microbiol, 174, 31-38.
  • 7. Balpetek D, Gürbüz Ü (2010): Investigations on the presence of E. coli O157: H7 in some meat products. Eurasian J Vet Sci, 26, 25-31.
  • 8. Bruttin A, Brüssow H (2005): Human volunteers receiving Escherichia coli phage T4 orally: a safety test of phage therapy. Antimicrob Agents Chemother, 49, 2874-2878.
  • 9. Brüssow H, Kutter E (2005): Phage ecology. 129-163. In: E Kutter, A Sulakvelizde (Eds), Bacteriophages: biology and applications. CRC Press, Washington DC.
  • 10. Büyükünal SK, Şakar FŞ, Turhan I, et al. (2016): Presence of Salmonella spp., Listeria monocytogenes, Escherichia coli 0157 and Nitrate-Nitrite Residue Levels in Turkish Traditional Fermented Meat Products (Sucuk and Pastirma). Kafkas Univ Vet Fak Derg, 22, 233-236.
  • 11. Chan BK, Abedon ST, Loc-Carrillo C (2013): Phage cocktails and the future of phage therapy. Future Microbiol, 8, 769-783.
  • 12. Erol İ (2007): Gıda Hijyeni ve Mikrobiyolojisi. Pozitif Matbaacılık, Ankara.
  • 13. Erol I, Goncuoglu M, Ayaz ND et al. (2016): Comparison of prevalence and genetic diversity of Escherichia coli O157:H7 in cattle and sheep. J Microbiol Biotechnol Food Sci, 6, 808-812.
  • 14. Gencay YE, Ayaz ND, Copuroglu G, et al. (2016): Biocontrol of Shiga Toxigenic Escherichia coliO157:H7 in Turkish Raw Meatball by Bacteriophage. J Food Saf, 36, 120-131.
  • 15. Guenther S, Huwyler D, Richard S, et al. (2009): Virulent bacteriophage for efficient biocontrol of Listeria monocytogenes in ready-to-eat foods. Appl Environ Microbiol, 75, 93-100.
  • 16. Hudson JA, Billington C, Cornelius A, et al. (2013): Use of a bacteriophage to inactivate Escherichia coli O157: H7 on beef. Food Microbiol, 36, 14-21.
  • 17. Kazi M, Annapure US. (2016): Bacteriophage biocontrol of foodborne pathogens. J Food Sci Technol, 53, 1355- 1362.
  • 18. Kudva IT, Jelacic S, Tarr PI, et al. (1999): Biocontrol of Escherichia coli O157 with O157-specific bacteriophages. Appl Environ Microbiol, 65, 3767-3773.
  • 19. Leverentz B, Conway WS, Camp MJ, et al. (2003): Biocontrol of Listeria monocytogenes on fresh-cut produce by treatment with lytic bacteriophages and a bacteriocin. Appl Environ Microbiol, 69, 4519-4526.
  • 20. Meng J, Doyle M, Zhao T, et al. (2001): Enterohemorrhagic Escherichia coli O157:H7. 193-213. In: MP Doyle, LR Beuchat, TJ Montville (Eds), Food Microbiology: Fundamentals and Frontiers. ASM press, Washington DC.
  • 21. O'Flaherty S, Ross RP, Coffey A (2009): Bacteriophage and their lysins for elimination of infectious bacteria. FEMS Microbiol Rev, 33, 801-819.
  • 22. O'Flynn G, Ross RP, Fitzgerald GF, et al. (2004): Evaluation of a cocktail of three bacteriophages for biocontrol of Escherichia coli O157:H7. Appl Environ Microbiol, 70, 3417-3424.
  • 23. Rozema EA, Stephens TP, Bach SJ, et al. (2009): Oral and rectal administration of bacteriophages for control of Escherichia coli O157: H7 in feedlot cattle. J Food Prot, 72, 241-250.
  • 24. Schmidt H, Beutin L, Karch H (1995): Molecular analysis of the plasmid-encoded hemolysin of Escherichia coli O157: H7 strain EDL 933. Infect immun, 63, 1055-1061.
  • 25. Sharma M, Patel JR, Conway WS, et al. (2009): Effectiveness of bacteriophages in reducing Escherichia coli O157: H7 on fresh-cut cantaloupes and lettuce. J Food Prot, 72, 1481-1485.
  • 26. Tanji Y, Shimada T, Yoichi M, et al. (2004): Toward rational control of Escherichia coli O157: H7 by a phage cocktail. Appl Microbiol Tech, 64, 270-274.
  • 27. Turkish Food Codex Legislation (2000): Et Ürünleri Tebliği. Resmi Gazete, 23960.
  • 28. US Food and Administration (2011): Inventory of effective food contact substance (FCS) notifications. FCN no. 1018. 2011.
  • 29. Viazis S, Akhtar M, Feirtag J, et al. (2011): Reduction of Escherichia coli O157: H7 viability on leafy green vegetables by treatment with a bacteriophage mixture and trans-cinnamaldehyde. Food Microbiol, 28,149-157.
  • 30. Viscardi M, Perugini AG, Auriemma C, et al. (2008): Isolation and characterisation of two novel coliphages with high potential to control antibiotic-resistant pathogenic Escherichia coli (EHEC and EPEC). Int J Antimicrob Agents, 31, 152-157.
There are 30 citations in total.

Details

Primary Language English
Journal Section Research Article
Authors

Gizem Çufaoğlu

Aşkın Nur Derinöz

Naim Deniz Ayaz

Publication Date December 31, 2018
Published in Issue Year 2019Volume: 66 Issue: 1

Cite

APA Çufaoğlu, G., Derinöz, A. N., & Ayaz, N. D. (2018). An investigation on biocontrol of Escherichia coli O157:H7 by a bacteriophage cocktail in pastirma. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 66(1), 7-11.
AMA Çufaoğlu G, Derinöz AN, Ayaz ND. An investigation on biocontrol of Escherichia coli O157:H7 by a bacteriophage cocktail in pastirma. Ankara Univ Vet Fak Derg. December 2018;66(1):7-11.
Chicago Çufaoğlu, Gizem, Aşkın Nur Derinöz, and Naim Deniz Ayaz. “An Investigation on Biocontrol of Escherichia Coli O157:H7 by a Bacteriophage Cocktail in Pastirma”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 66, no. 1 (December 2018): 7-11.
EndNote Çufaoğlu G, Derinöz AN, Ayaz ND (December 1, 2018) An investigation on biocontrol of Escherichia coli O157:H7 by a bacteriophage cocktail in pastirma. Ankara Üniversitesi Veteriner Fakültesi Dergisi 66 1 7–11.
IEEE G. Çufaoğlu, A. N. Derinöz, and N. D. Ayaz, “An investigation on biocontrol of Escherichia coli O157:H7 by a bacteriophage cocktail in pastirma”, Ankara Univ Vet Fak Derg, vol. 66, no. 1, pp. 7–11, 2018.
ISNAD Çufaoğlu, Gizem et al. “An Investigation on Biocontrol of Escherichia Coli O157:H7 by a Bacteriophage Cocktail in Pastirma”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 66/1 (December 2018), 7-11.
JAMA Çufaoğlu G, Derinöz AN, Ayaz ND. An investigation on biocontrol of Escherichia coli O157:H7 by a bacteriophage cocktail in pastirma. Ankara Univ Vet Fak Derg. 2018;66:7–11.
MLA Çufaoğlu, Gizem et al. “An Investigation on Biocontrol of Escherichia Coli O157:H7 by a Bacteriophage Cocktail in Pastirma”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 66, no. 1, 2018, pp. 7-11.
Vancouver Çufaoğlu G, Derinöz AN, Ayaz ND. An investigation on biocontrol of Escherichia coli O157:H7 by a bacteriophage cocktail in pastirma. Ankara Univ Vet Fak Derg. 2018;66(1):7-11.