Year 2019,
Volume: 66 Issue: 1, 43 - 52, 31.12.2018
Emre Kaya
,
Seval Yılmaz
Ali Osman Çeribaşı
Selda Telo
References
-
1. Abdallah IZ, Khattab HA (2004): Protective role of
lycopene against diethylnitrosamine induced experimental
hepatocarcinogenesis. Egypt J Hosp Med, 16, 1-13.
-
2. Aebi H (1974): Catalase. 673-8. In: Bergmeyer HU, editor.
Methods of enzymatic analysis. 2nd English ed. Weinheim:
Verlag Chemie.
-
3. Akyuz F, Inal M, Baycu C, et al, (2001): Changes in
antioxidant status and lipid peroxidation at liver and kidney tissues of the rats that were given diethylnitrosamine. Ann
Med Sci, 10(2), 50-54.
-
4. Astorg P, Gradelet S, Bergès R, et al. (1997): Dietary
lycopene decreases the initiation of liver preneoplastic foci
by diethylnitrosamine in the rat. Nutr. Cancer. 29(1), 60-68.
-
5. Atessahin A, Yilmaz S, Karahan I, et al. (2005): Effects
of lycopene against cisplatin-induced nephrotoxicity and
oxidative stress in rats. Toxicology, 212(2-3), 116-123.
-
6. Banakar MC, Paramasivan SK, Chattopadhyay MB, et
al. (2004): 1,25-Dihydroxyvitamin D3 prevents DNA
damage and restores antioxidant enzymes in rat
hepatocarcinogenesis induced by diethylnitrosamine and
promoted by phenobarbital. World J Gastroenterol, 10(9),
1268-1275.
-
7. Beutler E (1984): Red cell metabolism. 160. A manual of
biochemical methods. Grune and Starton (Editors) 2nd
Edition, New York.
-
8. Bingul I, Basaran-Kucukgergin C, Aydin AF, et al.
(2016): Blueberry treatment attenuated cirrhotic and
preneoplastic lesions and oxidative stress in the liver of
diethylnitrosamine-treated rats. Int J Immunopathol Pharm,
29(3), 426-437.
-
9. Bishayee A, Bhatia D, Thoppil RJ, et al. (2011):
Pomegranate-mediated chemoprevention of experimental
hepatocarcinogenesis involves Nrf2-regulated antioxidant
mechanisms. Carcinogenesis, 32(6), 888-896.
-
10. Bishayee A, Mbimba T, Thoppil RJ, et al. (2011):
Anthocyanin-rich black currant (Ribes nigrum L.) extract
affords chemoprevention against diethylnitrosamineinduced hepatocellular carcinogenesis in rats. J Nutr
Biochem, 22(11), 1035-1046.
-
11. Dalling JW, Pachen DMFA, Lousberg AHPJ, et al.
(1998:) Volatile N-nitrosamines in gastric juice of patients
with various conditions of the gastrointestinal tract
determined by gas chromatography–mass spectrometry and
related to intragastric pH and nitrate and nitrite levels.
Cancer Lett, 124(2), 119-125.
-
12. El-Gerbed MS (2014): Protective effect of lycopene on
deltamethrin-induced histological and ultrastructural
changes in kidney tissue of rats. Toxicol and Health, 30(2),
160-173
-
13. Ellman GL, Courtney KD, Andres V, et al. (1961): A new
and rapid colorimetric determination of
acetylcholinesterase activity. Biochem Pharmacol, 7, 88-95.
-
14. El-Mesallamy HO, Metwally NS, Soliman MS, et al.
(2011): The chemopreventive effect of Ginkgo biloba and
Silybum marianum extracts on hepatocarcinogenesis in
rats. Cancer Cell Int, 11(1), 38.
-
15. El-Shahat M, El-Abd S, Alkafafy M, et al. (2012):
Potential chemoprevention of diethylnitrosamine-induced
hepatocarcinogenesis in rats: myrrh (Commiphora molmol)
vs. turmeric (Curcuma longa). Acta Histochem, 114(5),
421-428.
-
16. Fridovich I (1986): Superoxide dismutases. Adv Enzymol,
58, 61-97.
-
17. Gayathri R, Priya DKD, Gunassekaran GR, et al.
(2009): Ursolic acid attenuates oxidative stress-mediated
hepatocellular carcinoma induction by diethylnitrosamine
in male Wistar rats. Asian Pacific J Cancer Prev, 10, 933-
938.
-
18. Giovannucci E (1999): Tomatoes, tomato-based products,
lycopene, and cancer: review of the epidemiologic
literature. J National Cancer Ins, 91(4), 317-331.
-
19. Habig WH, Pabst MJ, Jakoby WB (1974): Glutathione Stransferases. The first enzymatic step in mercapturic acid
formation. J Biol Chem, 249, 130-139.
-
20. Ip BC, Hu KQ, Liu C, et al. (2013): Lycopene metabolite,
apo-10′-lycopenoic acid, ınhibits diethylnitrosamine-
ınitiated, high fat diet–promoted hepatic ınflammation and
tumorigenesis in mice. Cancer Pre Res, 6(12), 1304-1316.
-
21. Jahan MS, Vani G, Shyamaladevi CS (2007): Effect of
Solanum trilobatum on hepatic drug metabolising enzymes
during diethylnitrosamine-induced hepatocarcinogenesis
promoted by Phenobarbital in rat. Hepatol Res, 37(1), 35-
49.
-
22. Kang JS, Wanibuchi H, Morimura K (2007): Role of
CYP2E1 in Diethylnitrosamine-induced hepatocarcinogenesis
in vivo. Cancer Res, 67(23), 11141-11146.
-
23. Karahan I, Yılmaz S (2006): Effects of prolonged low
amounts some nitrosoamines administrations on oxidative
stress in blood, liver and kidney of rats. Firat University
Veterinary Journal of Health Sciences, 20(1), 73-78.
-
24. Kubista M, Andrade JM, Bangtsson M, et al. (2006): The
real-time polymerase chain reaction. Mol Aspects Med,
27(2-3), 95-125.
-
25. Kumar P, Kumar A (2009): Effect of lycopene and
epigallocatechin-3-gallate against 3-nitropropionic acid
induced cognitive dysfunction and glutathione depletion in
rat: a novel nitric oxide mechanism. Food and Chem
Toxicol, 47(10), 2522-2530.
-
26. Lijinsky W (1992): Chemistry and Biology of N-nitroso
Compounds. Cambridge, UK: Cambridge Univ. Pres, 1992.
-
27. Lowry OH, Rosenbrough NJ, Farr A, et al. (1951):
Protein measurement with the folin-phenol reagent. J Biol
Chem, 193, 265-257.
-
28. Luna LG (1968): Manuel of histologic staining methods of
Armed Forces Institute of Pathology. 1-36, McGraw-Hill
Book Co, New York.
-
29. Man S, Fan W, Gao W, et al. (2014): Anti-fibrosis and
anti-cirrhosis effects of Rhizoma paridis saponins on
diethylnitrosamine induced rats. J Ethnopharmacol, 151(1),
407-412.
-
30. Matos HR, Capelozzi VL, Gomes OF, et al. (2001):
Lycopene inhibits DNA damage and liver necrosis in rats
treated with ferric nitrilotriacetate. Arch Biochem Biophys,
396, 171-177.
-
31. Ozkan E, Akyuz C, Dulundu E, et al. (2012): Protective
effects of lycopene on cerulein-induced experimental acute
pancreatitis in rats. J Surg Res, 176(1), 232-238.
-
32. Placer ZA, Cushman L, Johnson BC (1966): Estimation
of products of lipid peroxidation in biological fluids. Anal
Biochem, 16, 359-364.
-
33. Qu M, Nan X, Gao Z, et al. (2013): Protective effects of
lycopene against methylmercury-induced neurotoxicity in
cultured rat cerebellar granule neurons. Brain Res, 1540,
92-102.
-
34. Rao AV, Agarwal S (1999): Role of lycopene as
antioxidant carotenoid in the prevention of chronic
diseases. Nutr Res, 19, 199-203
-
35. Rehman A, Bourne LC, Halliwell B, et al. (1999): Tomato
consumption modulates oxidative DNA damage in humans.
Biochem Biophys Res Commun, 262, 828-831.
-
36. Rezaie A, Fazlara A, Karamolah MH, et al. (2013):
Effects of Echinacea purpurea on hepatic and renal toxicity
induced by diethylnitrosamine in rats. Jundishapur J Nat
Pharm Prod, 8(2), 60-64.
-
37. Sahin K, Orhan C, Tuzcu M, et al. (2014): Orally
administered lycopene attenuates diethylnitrosamineinduced hepatocarcinogenesis in rats by modulating Nrf-
2/HO-1 and Akt/mTOR pathways. Nutrition and Cancer,
66(4), 590-598.
-
38. Sayed-Ahmed MM, Aleisa AM, Al-Rejaie S, et al. (2010):
Thymoquinone attenuates diethylnitrosamine induction of
hepatic carcinogenesis through antioxidant signaling. Oxid
Med Cell Longev, 3, 254-261.
-
39. Sies H (1991): Oxidative Stress: Oxidants and Antioxidants.
Academic Press, San Diego, California
-
40. Sun Y, Oberly LW, Ying LA (1988): Simple method for
clinical assay of superoxide dismutase. Clin Chem, 34, 497-
500.
-
41. Yamada K, Yamamiya I, Utsumi H (2006): In vivo
detection of free radicals induced by diethylnitrosamine in
rat liver tissue. Free Radic Biol Med, 40, 2040-2046.
-
42. Yilmaz S, Atessahin A, Sahna E, et al. (2006): Protective
effect of lycopene on adriamycin-induced cardiotoxicity and
nephrotoxicity. Toxicology, 218(2), 164-171
Protective effect of lycopene on diethylnitrosamine-induced oxidative stress and catalase expression in rats
Year 2019,
Volume: 66 Issue: 1, 43 - 52, 31.12.2018
Emre Kaya
,
Seval Yılmaz
Ali Osman Çeribaşı
Selda Telo
Abstract
The purpose of this study is to investigate the protective role of lycopene on diethylnitrosamine (DEN)-induced
hepatotoxicity using biochemical and histopathological approaches. The rats were divided into 5 groups as control, lycopene, DEN,
lycopene+DEN and DEN+lycopene. DEN was administered to rats at 200 mg/kg, a single dose intraperitoneally for 30 days. Lycopene
was administered to rats every other day at 10 mg/kg, gavage for 10 days. Lycopene administration was started 10 days before the
DEN administration in lycopene+DEN group and together with the DEN administration in DEN+lycopene group. In this study,
malondialdehyde (MDA), reduced glutathione (GSH) levels, catalase (CAT), glutathione peroxidase (GSH-Px), glutathione-Stransferase (GST), superoxide dismutase (SOD) activities and the expression levels of the CAT enzyme were measured in blood and
liver tissues. DEN caused the oxidative stress by the increased MDA level and the reduced GSH level, antioxidant enzyme activities
in tissues. Lycopene administration produced amelioration in biochemical indices of hepatotoxicity in both blood and liver tissues
when compared to DEN group; simultaneous-administration with DEN has been more effective. It was determined to increase
expression levels of the CAT enzyme in the DEN group in RT-PCR. The improvement in expression levels in DEN+lycopene group
was observed to be better than the lycopene+DEN group. Histopathologically, many different histopathological changes were observed
in liver tissues of DEN and lycopene+DEN groups, it was determined that these changes reduced in DEN+lycopene group. The results
from the present study indicate that lycopene exhibits good hepatoprotective and antioxidant potential against DEN-induced
hepatocellular damage in rats.
References
-
1. Abdallah IZ, Khattab HA (2004): Protective role of
lycopene against diethylnitrosamine induced experimental
hepatocarcinogenesis. Egypt J Hosp Med, 16, 1-13.
-
2. Aebi H (1974): Catalase. 673-8. In: Bergmeyer HU, editor.
Methods of enzymatic analysis. 2nd English ed. Weinheim:
Verlag Chemie.
-
3. Akyuz F, Inal M, Baycu C, et al, (2001): Changes in
antioxidant status and lipid peroxidation at liver and kidney tissues of the rats that were given diethylnitrosamine. Ann
Med Sci, 10(2), 50-54.
-
4. Astorg P, Gradelet S, Bergès R, et al. (1997): Dietary
lycopene decreases the initiation of liver preneoplastic foci
by diethylnitrosamine in the rat. Nutr. Cancer. 29(1), 60-68.
-
5. Atessahin A, Yilmaz S, Karahan I, et al. (2005): Effects
of lycopene against cisplatin-induced nephrotoxicity and
oxidative stress in rats. Toxicology, 212(2-3), 116-123.
-
6. Banakar MC, Paramasivan SK, Chattopadhyay MB, et
al. (2004): 1,25-Dihydroxyvitamin D3 prevents DNA
damage and restores antioxidant enzymes in rat
hepatocarcinogenesis induced by diethylnitrosamine and
promoted by phenobarbital. World J Gastroenterol, 10(9),
1268-1275.
-
7. Beutler E (1984): Red cell metabolism. 160. A manual of
biochemical methods. Grune and Starton (Editors) 2nd
Edition, New York.
-
8. Bingul I, Basaran-Kucukgergin C, Aydin AF, et al.
(2016): Blueberry treatment attenuated cirrhotic and
preneoplastic lesions and oxidative stress in the liver of
diethylnitrosamine-treated rats. Int J Immunopathol Pharm,
29(3), 426-437.
-
9. Bishayee A, Bhatia D, Thoppil RJ, et al. (2011):
Pomegranate-mediated chemoprevention of experimental
hepatocarcinogenesis involves Nrf2-regulated antioxidant
mechanisms. Carcinogenesis, 32(6), 888-896.
-
10. Bishayee A, Mbimba T, Thoppil RJ, et al. (2011):
Anthocyanin-rich black currant (Ribes nigrum L.) extract
affords chemoprevention against diethylnitrosamineinduced hepatocellular carcinogenesis in rats. J Nutr
Biochem, 22(11), 1035-1046.
-
11. Dalling JW, Pachen DMFA, Lousberg AHPJ, et al.
(1998:) Volatile N-nitrosamines in gastric juice of patients
with various conditions of the gastrointestinal tract
determined by gas chromatography–mass spectrometry and
related to intragastric pH and nitrate and nitrite levels.
Cancer Lett, 124(2), 119-125.
-
12. El-Gerbed MS (2014): Protective effect of lycopene on
deltamethrin-induced histological and ultrastructural
changes in kidney tissue of rats. Toxicol and Health, 30(2),
160-173
-
13. Ellman GL, Courtney KD, Andres V, et al. (1961): A new
and rapid colorimetric determination of
acetylcholinesterase activity. Biochem Pharmacol, 7, 88-95.
-
14. El-Mesallamy HO, Metwally NS, Soliman MS, et al.
(2011): The chemopreventive effect of Ginkgo biloba and
Silybum marianum extracts on hepatocarcinogenesis in
rats. Cancer Cell Int, 11(1), 38.
-
15. El-Shahat M, El-Abd S, Alkafafy M, et al. (2012):
Potential chemoprevention of diethylnitrosamine-induced
hepatocarcinogenesis in rats: myrrh (Commiphora molmol)
vs. turmeric (Curcuma longa). Acta Histochem, 114(5),
421-428.
-
16. Fridovich I (1986): Superoxide dismutases. Adv Enzymol,
58, 61-97.
-
17. Gayathri R, Priya DKD, Gunassekaran GR, et al.
(2009): Ursolic acid attenuates oxidative stress-mediated
hepatocellular carcinoma induction by diethylnitrosamine
in male Wistar rats. Asian Pacific J Cancer Prev, 10, 933-
938.
-
18. Giovannucci E (1999): Tomatoes, tomato-based products,
lycopene, and cancer: review of the epidemiologic
literature. J National Cancer Ins, 91(4), 317-331.
-
19. Habig WH, Pabst MJ, Jakoby WB (1974): Glutathione Stransferases. The first enzymatic step in mercapturic acid
formation. J Biol Chem, 249, 130-139.
-
20. Ip BC, Hu KQ, Liu C, et al. (2013): Lycopene metabolite,
apo-10′-lycopenoic acid, ınhibits diethylnitrosamine-
ınitiated, high fat diet–promoted hepatic ınflammation and
tumorigenesis in mice. Cancer Pre Res, 6(12), 1304-1316.
-
21. Jahan MS, Vani G, Shyamaladevi CS (2007): Effect of
Solanum trilobatum on hepatic drug metabolising enzymes
during diethylnitrosamine-induced hepatocarcinogenesis
promoted by Phenobarbital in rat. Hepatol Res, 37(1), 35-
49.
-
22. Kang JS, Wanibuchi H, Morimura K (2007): Role of
CYP2E1 in Diethylnitrosamine-induced hepatocarcinogenesis
in vivo. Cancer Res, 67(23), 11141-11146.
-
23. Karahan I, Yılmaz S (2006): Effects of prolonged low
amounts some nitrosoamines administrations on oxidative
stress in blood, liver and kidney of rats. Firat University
Veterinary Journal of Health Sciences, 20(1), 73-78.
-
24. Kubista M, Andrade JM, Bangtsson M, et al. (2006): The
real-time polymerase chain reaction. Mol Aspects Med,
27(2-3), 95-125.
-
25. Kumar P, Kumar A (2009): Effect of lycopene and
epigallocatechin-3-gallate against 3-nitropropionic acid
induced cognitive dysfunction and glutathione depletion in
rat: a novel nitric oxide mechanism. Food and Chem
Toxicol, 47(10), 2522-2530.
-
26. Lijinsky W (1992): Chemistry and Biology of N-nitroso
Compounds. Cambridge, UK: Cambridge Univ. Pres, 1992.
-
27. Lowry OH, Rosenbrough NJ, Farr A, et al. (1951):
Protein measurement with the folin-phenol reagent. J Biol
Chem, 193, 265-257.
-
28. Luna LG (1968): Manuel of histologic staining methods of
Armed Forces Institute of Pathology. 1-36, McGraw-Hill
Book Co, New York.
-
29. Man S, Fan W, Gao W, et al. (2014): Anti-fibrosis and
anti-cirrhosis effects of Rhizoma paridis saponins on
diethylnitrosamine induced rats. J Ethnopharmacol, 151(1),
407-412.
-
30. Matos HR, Capelozzi VL, Gomes OF, et al. (2001):
Lycopene inhibits DNA damage and liver necrosis in rats
treated with ferric nitrilotriacetate. Arch Biochem Biophys,
396, 171-177.
-
31. Ozkan E, Akyuz C, Dulundu E, et al. (2012): Protective
effects of lycopene on cerulein-induced experimental acute
pancreatitis in rats. J Surg Res, 176(1), 232-238.
-
32. Placer ZA, Cushman L, Johnson BC (1966): Estimation
of products of lipid peroxidation in biological fluids. Anal
Biochem, 16, 359-364.
-
33. Qu M, Nan X, Gao Z, et al. (2013): Protective effects of
lycopene against methylmercury-induced neurotoxicity in
cultured rat cerebellar granule neurons. Brain Res, 1540,
92-102.
-
34. Rao AV, Agarwal S (1999): Role of lycopene as
antioxidant carotenoid in the prevention of chronic
diseases. Nutr Res, 19, 199-203
-
35. Rehman A, Bourne LC, Halliwell B, et al. (1999): Tomato
consumption modulates oxidative DNA damage in humans.
Biochem Biophys Res Commun, 262, 828-831.
-
36. Rezaie A, Fazlara A, Karamolah MH, et al. (2013):
Effects of Echinacea purpurea on hepatic and renal toxicity
induced by diethylnitrosamine in rats. Jundishapur J Nat
Pharm Prod, 8(2), 60-64.
-
37. Sahin K, Orhan C, Tuzcu M, et al. (2014): Orally
administered lycopene attenuates diethylnitrosamineinduced hepatocarcinogenesis in rats by modulating Nrf-
2/HO-1 and Akt/mTOR pathways. Nutrition and Cancer,
66(4), 590-598.
-
38. Sayed-Ahmed MM, Aleisa AM, Al-Rejaie S, et al. (2010):
Thymoquinone attenuates diethylnitrosamine induction of
hepatic carcinogenesis through antioxidant signaling. Oxid
Med Cell Longev, 3, 254-261.
-
39. Sies H (1991): Oxidative Stress: Oxidants and Antioxidants.
Academic Press, San Diego, California
-
40. Sun Y, Oberly LW, Ying LA (1988): Simple method for
clinical assay of superoxide dismutase. Clin Chem, 34, 497-
500.
-
41. Yamada K, Yamamiya I, Utsumi H (2006): In vivo
detection of free radicals induced by diethylnitrosamine in
rat liver tissue. Free Radic Biol Med, 40, 2040-2046.
-
42. Yilmaz S, Atessahin A, Sahna E, et al. (2006): Protective
effect of lycopene on adriamycin-induced cardiotoxicity and
nephrotoxicity. Toxicology, 218(2), 164-171