Protective effect of lycopene on diethylnitrosamine-induced oxidative stress and catalase expression in rats

Emre Kaya; Seval Yılmaz; Ali Osman Çeribaşı; Selda Telo

Research Article

Year 2019, Volume: 66 Issue: 1, 43 - 52, 31.12.2018

Emre Kaya , Seval Yılmaz Ali Osman Çeribaşı Selda Telo

Abstract

References

1. Abdallah IZ, Khattab HA (2004): Protective role of lycopene against diethylnitrosamine induced experimental hepatocarcinogenesis. Egypt J Hosp Med, 16, 1-13.
2. Aebi H (1974): Catalase. 673-8. In: Bergmeyer HU, editor. Methods of enzymatic analysis. 2nd English ed. Weinheim: Verlag Chemie.
3. Akyuz F, Inal M, Baycu C, et al, (2001): Changes in antioxidant status and lipid peroxidation at liver and kidney tissues of the rats that were given diethylnitrosamine. Ann Med Sci, 10(2), 50-54.
4. Astorg P, Gradelet S, Bergès R, et al. (1997): Dietary lycopene decreases the initiation of liver preneoplastic foci by diethylnitrosamine in the rat. Nutr. Cancer. 29(1), 60-68.
5. Atessahin A, Yilmaz S, Karahan I, et al. (2005): Effects of lycopene against cisplatin-induced nephrotoxicity and oxidative stress in rats. Toxicology, 212(2-3), 116-123.
6. Banakar MC, Paramasivan SK, Chattopadhyay MB, et al. (2004): 1,25-Dihydroxyvitamin D3 prevents DNA damage and restores antioxidant enzymes in rat hepatocarcinogenesis induced by diethylnitrosamine and promoted by phenobarbital. World J Gastroenterol, 10(9), 1268-1275.
7. Beutler E (1984): Red cell metabolism. 160. A manual of biochemical methods. Grune and Starton (Editors) 2nd Edition, New York.
8. Bingul I, Basaran-Kucukgergin C, Aydin AF, et al. (2016): Blueberry treatment attenuated cirrhotic and preneoplastic lesions and oxidative stress in the liver of diethylnitrosamine-treated rats. Int J Immunopathol Pharm, 29(3), 426-437.
9. Bishayee A, Bhatia D, Thoppil RJ, et al. (2011): Pomegranate-mediated chemoprevention of experimental hepatocarcinogenesis involves Nrf2-regulated antioxidant mechanisms. Carcinogenesis, 32(6), 888-896.
10. Bishayee A, Mbimba T, Thoppil RJ, et al. (2011): Anthocyanin-rich black currant (Ribes nigrum L.) extract affords chemoprevention against diethylnitrosamineinduced hepatocellular carcinogenesis in rats. J Nutr Biochem, 22(11), 1035-1046.
11. Dalling JW, Pachen DMFA, Lousberg AHPJ, et al. (1998:) Volatile N-nitrosamines in gastric juice of patients with various conditions of the gastrointestinal tract determined by gas chromatography–mass spectrometry and related to intragastric pH and nitrate and nitrite levels. Cancer Lett, 124(2), 119-125.
12. El-Gerbed MS (2014): Protective effect of lycopene on deltamethrin-induced histological and ultrastructural changes in kidney tissue of rats. Toxicol and Health, 30(2), 160-173
13. Ellman GL, Courtney KD, Andres V, et al. (1961): A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol, 7, 88-95.
14. El-Mesallamy HO, Metwally NS, Soliman MS, et al. (2011): The chemopreventive effect of Ginkgo biloba and Silybum marianum extracts on hepatocarcinogenesis in rats. Cancer Cell Int, 11(1), 38.
15. El-Shahat M, El-Abd S, Alkafafy M, et al. (2012): Potential chemoprevention of diethylnitrosamine-induced hepatocarcinogenesis in rats: myrrh (Commiphora molmol) vs. turmeric (Curcuma longa). Acta Histochem, 114(5), 421-428.
16. Fridovich I (1986): Superoxide dismutases. Adv Enzymol, 58, 61-97.
17. Gayathri R, Priya DKD, Gunassekaran GR, et al. (2009): Ursolic acid attenuates oxidative stress-mediated hepatocellular carcinoma induction by diethylnitrosamine in male Wistar rats. Asian Pacific J Cancer Prev, 10, 933- 938.
18. Giovannucci E (1999): Tomatoes, tomato-based products, lycopene, and cancer: review of the epidemiologic literature. J National Cancer Ins, 91(4), 317-331.
19. Habig WH, Pabst MJ, Jakoby WB (1974): Glutathione Stransferases. The first enzymatic step in mercapturic acid formation. J Biol Chem, 249, 130-139.
20. Ip BC, Hu KQ, Liu C, et al. (2013): Lycopene metabolite, apo-10′-lycopenoic acid, ınhibits diethylnitrosamine- ınitiated, high fat diet–promoted hepatic ınflammation and tumorigenesis in mice. Cancer Pre Res, 6(12), 1304-1316.
21. Jahan MS, Vani G, Shyamaladevi CS (2007): Effect of Solanum trilobatum on hepatic drug metabolising enzymes during diethylnitrosamine-induced hepatocarcinogenesis promoted by Phenobarbital in rat. Hepatol Res, 37(1), 35- 49.
22. Kang JS, Wanibuchi H, Morimura K (2007): Role of CYP2E1 in Diethylnitrosamine-induced hepatocarcinogenesis in vivo. Cancer Res, 67(23), 11141-11146.
23. Karahan I, Yılmaz S (2006): Effects of prolonged low amounts some nitrosoamines administrations on oxidative stress in blood, liver and kidney of rats. Firat University Veterinary Journal of Health Sciences, 20(1), 73-78.
24. Kubista M, Andrade JM, Bangtsson M, et al. (2006): The real-time polymerase chain reaction. Mol Aspects Med, 27(2-3), 95-125.
25. Kumar P, Kumar A (2009): Effect of lycopene and epigallocatechin-3-gallate against 3-nitropropionic acid induced cognitive dysfunction and glutathione depletion in rat: a novel nitric oxide mechanism. Food and Chem Toxicol, 47(10), 2522-2530.
26. Lijinsky W (1992): Chemistry and Biology of N-nitroso Compounds. Cambridge, UK: Cambridge Univ. Pres, 1992.
27. Lowry OH, Rosenbrough NJ, Farr A, et al. (1951): Protein measurement with the folin-phenol reagent. J Biol Chem, 193, 265-257.
28. Luna LG (1968): Manuel of histologic staining methods of Armed Forces Institute of Pathology. 1-36, McGraw-Hill Book Co, New York.
29. Man S, Fan W, Gao W, et al. (2014): Anti-fibrosis and anti-cirrhosis effects of Rhizoma paridis saponins on diethylnitrosamine induced rats. J Ethnopharmacol, 151(1), 407-412.
30. Matos HR, Capelozzi VL, Gomes OF, et al. (2001): Lycopene inhibits DNA damage and liver necrosis in rats treated with ferric nitrilotriacetate. Arch Biochem Biophys, 396, 171-177.
31. Ozkan E, Akyuz C, Dulundu E, et al. (2012): Protective effects of lycopene on cerulein-induced experimental acute pancreatitis in rats. J Surg Res, 176(1), 232-238.
32. Placer ZA, Cushman L, Johnson BC (1966): Estimation of products of lipid peroxidation in biological fluids. Anal Biochem, 16, 359-364.
33. Qu M, Nan X, Gao Z, et al. (2013): Protective effects of lycopene against methylmercury-induced neurotoxicity in cultured rat cerebellar granule neurons. Brain Res, 1540, 92-102.
34. Rao AV, Agarwal S (1999): Role of lycopene as antioxidant carotenoid in the prevention of chronic diseases. Nutr Res, 19, 199-203
35. Rehman A, Bourne LC, Halliwell B, et al. (1999): Tomato consumption modulates oxidative DNA damage in humans. Biochem Biophys Res Commun, 262, 828-831.
36. Rezaie A, Fazlara A, Karamolah MH, et al. (2013): Effects of Echinacea purpurea on hepatic and renal toxicity induced by diethylnitrosamine in rats. Jundishapur J Nat Pharm Prod, 8(2), 60-64.
37. Sahin K, Orhan C, Tuzcu M, et al. (2014): Orally administered lycopene attenuates diethylnitrosamineinduced hepatocarcinogenesis in rats by modulating Nrf- 2/HO-1 and Akt/mTOR pathways. Nutrition and Cancer, 66(4), 590-598.
38. Sayed-Ahmed MM, Aleisa AM, Al-Rejaie S, et al. (2010): Thymoquinone attenuates diethylnitrosamine induction of hepatic carcinogenesis through antioxidant signaling. Oxid Med Cell Longev, 3, 254-261.
39. Sies H (1991): Oxidative Stress: Oxidants and Antioxidants. Academic Press, San Diego, California
40. Sun Y, Oberly LW, Ying LA (1988): Simple method for clinical assay of superoxide dismutase. Clin Chem, 34, 497- 500.
41. Yamada K, Yamamiya I, Utsumi H (2006): In vivo detection of free radicals induced by diethylnitrosamine in rat liver tissue. Free Radic Biol Med, 40, 2040-2046.
42. Yilmaz S, Atessahin A, Sahna E, et al. (2006): Protective effect of lycopene on adriamycin-induced cardiotoxicity and nephrotoxicity. Toxicology, 218(2), 164-171

Protective effect of lycopene on diethylnitrosamine-induced oxidative stress and catalase expression in rats

Year 2019, Volume: 66 Issue: 1, 43 - 52, 31.12.2018

Emre Kaya , Seval Yılmaz Ali Osman Çeribaşı Selda Telo

Abstract

The purpose of this study is to investigate the protective role of lycopene on diethylnitrosamine (DEN)-induced
hepatotoxicity using biochemical and histopathological approaches. The rats were divided into 5 groups as control, lycopene, DEN,
lycopene+DEN and DEN+lycopene. DEN was administered to rats at 200 mg/kg, a single dose intraperitoneally for 30 days. Lycopene
was administered to rats every other day at 10 mg/kg, gavage for 10 days. Lycopene administration was started 10 days before the
DEN administration in lycopene+DEN group and together with the DEN administration in DEN+lycopene group. In this study,
malondialdehyde (MDA), reduced glutathione (GSH) levels, catalase (CAT), glutathione peroxidase (GSH-Px), glutathione-Stransferase (GST), superoxide dismutase (SOD) activities and the expression levels of the CAT enzyme were measured in blood and
liver tissues. DEN caused the oxidative stress by the increased MDA level and the reduced GSH level, antioxidant enzyme activities
in tissues. Lycopene administration produced amelioration in biochemical indices of hepatotoxicity in both blood and liver tissues
when compared to DEN group; simultaneous-administration with DEN has been more effective. It was determined to increase
expression levels of the CAT enzyme in the DEN group in RT-PCR. The improvement in expression levels in DEN+lycopene group
was observed to be better than the lycopene+DEN group. Histopathologically, many different histopathological changes were observed
in liver tissues of DEN and lycopene+DEN groups, it was determined that these changes reduced in DEN+lycopene group. The results
from the present study indicate that lycopene exhibits good hepatoprotective and antioxidant potential against DEN-induced
hepatocellular damage in rats.

Keywords

Antioxidant, CAT expression, diethylnitrosamine, lycopene, oxidative stress

References

1. Abdallah IZ, Khattab HA (2004): Protective role of lycopene against diethylnitrosamine induced experimental hepatocarcinogenesis. Egypt J Hosp Med, 16, 1-13.
2. Aebi H (1974): Catalase. 673-8. In: Bergmeyer HU, editor. Methods of enzymatic analysis. 2nd English ed. Weinheim: Verlag Chemie.
3. Akyuz F, Inal M, Baycu C, et al, (2001): Changes in antioxidant status and lipid peroxidation at liver and kidney tissues of the rats that were given diethylnitrosamine. Ann Med Sci, 10(2), 50-54.
4. Astorg P, Gradelet S, Bergès R, et al. (1997): Dietary lycopene decreases the initiation of liver preneoplastic foci by diethylnitrosamine in the rat. Nutr. Cancer. 29(1), 60-68.
5. Atessahin A, Yilmaz S, Karahan I, et al. (2005): Effects of lycopene against cisplatin-induced nephrotoxicity and oxidative stress in rats. Toxicology, 212(2-3), 116-123.
6. Banakar MC, Paramasivan SK, Chattopadhyay MB, et al. (2004): 1,25-Dihydroxyvitamin D3 prevents DNA damage and restores antioxidant enzymes in rat hepatocarcinogenesis induced by diethylnitrosamine and promoted by phenobarbital. World J Gastroenterol, 10(9), 1268-1275.
7. Beutler E (1984): Red cell metabolism. 160. A manual of biochemical methods. Grune and Starton (Editors) 2nd Edition, New York.
8. Bingul I, Basaran-Kucukgergin C, Aydin AF, et al. (2016): Blueberry treatment attenuated cirrhotic and preneoplastic lesions and oxidative stress in the liver of diethylnitrosamine-treated rats. Int J Immunopathol Pharm, 29(3), 426-437.
9. Bishayee A, Bhatia D, Thoppil RJ, et al. (2011): Pomegranate-mediated chemoprevention of experimental hepatocarcinogenesis involves Nrf2-regulated antioxidant mechanisms. Carcinogenesis, 32(6), 888-896.
10. Bishayee A, Mbimba T, Thoppil RJ, et al. (2011): Anthocyanin-rich black currant (Ribes nigrum L.) extract affords chemoprevention against diethylnitrosamineinduced hepatocellular carcinogenesis in rats. J Nutr Biochem, 22(11), 1035-1046.
11. Dalling JW, Pachen DMFA, Lousberg AHPJ, et al. (1998:) Volatile N-nitrosamines in gastric juice of patients with various conditions of the gastrointestinal tract determined by gas chromatography–mass spectrometry and related to intragastric pH and nitrate and nitrite levels. Cancer Lett, 124(2), 119-125.
12. El-Gerbed MS (2014): Protective effect of lycopene on deltamethrin-induced histological and ultrastructural changes in kidney tissue of rats. Toxicol and Health, 30(2), 160-173
13. Ellman GL, Courtney KD, Andres V, et al. (1961): A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol, 7, 88-95.
14. El-Mesallamy HO, Metwally NS, Soliman MS, et al. (2011): The chemopreventive effect of Ginkgo biloba and Silybum marianum extracts on hepatocarcinogenesis in rats. Cancer Cell Int, 11(1), 38.
15. El-Shahat M, El-Abd S, Alkafafy M, et al. (2012): Potential chemoprevention of diethylnitrosamine-induced hepatocarcinogenesis in rats: myrrh (Commiphora molmol) vs. turmeric (Curcuma longa). Acta Histochem, 114(5), 421-428.
16. Fridovich I (1986): Superoxide dismutases. Adv Enzymol, 58, 61-97.
17. Gayathri R, Priya DKD, Gunassekaran GR, et al. (2009): Ursolic acid attenuates oxidative stress-mediated hepatocellular carcinoma induction by diethylnitrosamine in male Wistar rats. Asian Pacific J Cancer Prev, 10, 933- 938.
18. Giovannucci E (1999): Tomatoes, tomato-based products, lycopene, and cancer: review of the epidemiologic literature. J National Cancer Ins, 91(4), 317-331.
19. Habig WH, Pabst MJ, Jakoby WB (1974): Glutathione Stransferases. The first enzymatic step in mercapturic acid formation. J Biol Chem, 249, 130-139.
20. Ip BC, Hu KQ, Liu C, et al. (2013): Lycopene metabolite, apo-10′-lycopenoic acid, ınhibits diethylnitrosamine- ınitiated, high fat diet–promoted hepatic ınflammation and tumorigenesis in mice. Cancer Pre Res, 6(12), 1304-1316.
21. Jahan MS, Vani G, Shyamaladevi CS (2007): Effect of Solanum trilobatum on hepatic drug metabolising enzymes during diethylnitrosamine-induced hepatocarcinogenesis promoted by Phenobarbital in rat. Hepatol Res, 37(1), 35- 49.
22. Kang JS, Wanibuchi H, Morimura K (2007): Role of CYP2E1 in Diethylnitrosamine-induced hepatocarcinogenesis in vivo. Cancer Res, 67(23), 11141-11146.
23. Karahan I, Yılmaz S (2006): Effects of prolonged low amounts some nitrosoamines administrations on oxidative stress in blood, liver and kidney of rats. Firat University Veterinary Journal of Health Sciences, 20(1), 73-78.
24. Kubista M, Andrade JM, Bangtsson M, et al. (2006): The real-time polymerase chain reaction. Mol Aspects Med, 27(2-3), 95-125.
25. Kumar P, Kumar A (2009): Effect of lycopene and epigallocatechin-3-gallate against 3-nitropropionic acid induced cognitive dysfunction and glutathione depletion in rat: a novel nitric oxide mechanism. Food and Chem Toxicol, 47(10), 2522-2530.
26. Lijinsky W (1992): Chemistry and Biology of N-nitroso Compounds. Cambridge, UK: Cambridge Univ. Pres, 1992.
27. Lowry OH, Rosenbrough NJ, Farr A, et al. (1951): Protein measurement with the folin-phenol reagent. J Biol Chem, 193, 265-257.
28. Luna LG (1968): Manuel of histologic staining methods of Armed Forces Institute of Pathology. 1-36, McGraw-Hill Book Co, New York.
29. Man S, Fan W, Gao W, et al. (2014): Anti-fibrosis and anti-cirrhosis effects of Rhizoma paridis saponins on diethylnitrosamine induced rats. J Ethnopharmacol, 151(1), 407-412.
30. Matos HR, Capelozzi VL, Gomes OF, et al. (2001): Lycopene inhibits DNA damage and liver necrosis in rats treated with ferric nitrilotriacetate. Arch Biochem Biophys, 396, 171-177.
31. Ozkan E, Akyuz C, Dulundu E, et al. (2012): Protective effects of lycopene on cerulein-induced experimental acute pancreatitis in rats. J Surg Res, 176(1), 232-238.
32. Placer ZA, Cushman L, Johnson BC (1966): Estimation of products of lipid peroxidation in biological fluids. Anal Biochem, 16, 359-364.
33. Qu M, Nan X, Gao Z, et al. (2013): Protective effects of lycopene against methylmercury-induced neurotoxicity in cultured rat cerebellar granule neurons. Brain Res, 1540, 92-102.
34. Rao AV, Agarwal S (1999): Role of lycopene as antioxidant carotenoid in the prevention of chronic diseases. Nutr Res, 19, 199-203
35. Rehman A, Bourne LC, Halliwell B, et al. (1999): Tomato consumption modulates oxidative DNA damage in humans. Biochem Biophys Res Commun, 262, 828-831.
36. Rezaie A, Fazlara A, Karamolah MH, et al. (2013): Effects of Echinacea purpurea on hepatic and renal toxicity induced by diethylnitrosamine in rats. Jundishapur J Nat Pharm Prod, 8(2), 60-64.
37. Sahin K, Orhan C, Tuzcu M, et al. (2014): Orally administered lycopene attenuates diethylnitrosamineinduced hepatocarcinogenesis in rats by modulating Nrf- 2/HO-1 and Akt/mTOR pathways. Nutrition and Cancer, 66(4), 590-598.
38. Sayed-Ahmed MM, Aleisa AM, Al-Rejaie S, et al. (2010): Thymoquinone attenuates diethylnitrosamine induction of hepatic carcinogenesis through antioxidant signaling. Oxid Med Cell Longev, 3, 254-261.
39. Sies H (1991): Oxidative Stress: Oxidants and Antioxidants. Academic Press, San Diego, California
40. Sun Y, Oberly LW, Ying LA (1988): Simple method for clinical assay of superoxide dismutase. Clin Chem, 34, 497- 500.
41. Yamada K, Yamamiya I, Utsumi H (2006): In vivo detection of free radicals induced by diethylnitrosamine in rat liver tissue. Free Radic Biol Med, 40, 2040-2046.
42. Yilmaz S, Atessahin A, Sahna E, et al. (2006): Protective effect of lycopene on adriamycin-induced cardiotoxicity and nephrotoxicity. Toxicology, 218(2), 164-171

There are 42 citations in total.

Details

Primary Language	English
Journal Section	Research Article
Authors	Emre Kaya Seval Yılmaz Ali Osman Çeribaşı Selda Telo
Publication Date	December 31, 2018
Published in Issue	Year 2019Volume: 66 Issue: 1

Cite

APA	Kaya, E., Yılmaz, S., Çeribaşı, A. O., Telo, S. (2018). Protective effect of lycopene on diethylnitrosamine-induced oxidative stress and catalase expression in rats. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 66(1), 43-52.
AMA	Kaya E, Yılmaz S, Çeribaşı AO, Telo S. Protective effect of lycopene on diethylnitrosamine-induced oxidative stress and catalase expression in rats. Ankara Univ Vet Fak Derg. December 2018;66(1):43-52.
Chicago	Kaya, Emre, Seval Yılmaz, Ali Osman Çeribaşı, and Selda Telo. “Protective Effect of Lycopene on Diethylnitrosamine-Induced Oxidative Stress and Catalase Expression in Rats”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 66, no. 1 (December 2018): 43-52.
EndNote	Kaya E, Yılmaz S, Çeribaşı AO, Telo S (December 1, 2018) Protective effect of lycopene on diethylnitrosamine-induced oxidative stress and catalase expression in rats. Ankara Üniversitesi Veteriner Fakültesi Dergisi 66 1 43–52.
IEEE	E. Kaya, S. Yılmaz, A. O. Çeribaşı, and S. Telo, “Protective effect of lycopene on diethylnitrosamine-induced oxidative stress and catalase expression in rats”, Ankara Univ Vet Fak Derg, vol. 66, no. 1, pp. 43–52, 2018.
ISNAD	Kaya, Emre et al. “Protective Effect of Lycopene on Diethylnitrosamine-Induced Oxidative Stress and Catalase Expression in Rats”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 66/1 (December 2018), 43-52.
JAMA	Kaya E, Yılmaz S, Çeribaşı AO, Telo S. Protective effect of lycopene on diethylnitrosamine-induced oxidative stress and catalase expression in rats. Ankara Univ Vet Fak Derg. 2018;66:43–52.
MLA	Kaya, Emre et al. “Protective Effect of Lycopene on Diethylnitrosamine-Induced Oxidative Stress and Catalase Expression in Rats”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 66, no. 1, 2018, pp. 43-52.
Vancouver	Kaya E, Yılmaz S, Çeribaşı AO, Telo S. Protective effect of lycopene on diethylnitrosamine-induced oxidative stress and catalase expression in rats. Ankara Univ Vet Fak Derg. 2018;66(1):43-52.

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