Research Article
BibTex RIS Cite

Year 2019, Volume: 66 Issue: 1, 53 - 58, 31.12.2018

Oktay Genç , Gülnur Serdar Özlem Büyüktanır Yaş Evrim Genç

Abstract

References

  • 1. Aragon V, Diaz R, Moreno E, et al. (1996): Characterization of B. abortus and B. melitensis Native Haptens as Outer Membrane O-Type Polysaccharides Independent from the Smooth Lipopolysaccharide. J Bacteriology, 178(4), 1070–1079.
  • 2. Barquero-Calvo E, Chaves-Olarte E, Weiss DS, et al. (2007): B. abortus uses a stealthy strategy to avoid activation of the innate immune system during the onset of infection. PLoS One. 2:e631.
  • 3. Brooks-Worrell BM, Splitter GA (1992):Sodium dodecyl sulfate and salt extracted antigens from various Brucella species induce proliferation of bovine lymphocytes. Infect Immun, 60(5), 2136-2138.
  • 4. Caroff M, Bundle DR, Perry MB, et al. (1984):Antigenic S-type lipopolysaccharide of Brucella abortus 1119-3. Infect Immun; 46, 384-388.
  • 5. Cha S-B, Rayamajhi N, Kang M-L, et al. (2010): Comparative study of gamma interferon production in mice immunized with outer membrane proteins and whole bacteria of Brucella abortus. Jpn J Infect Dis, 63, 49-51.
  • 6. Chand P, Chhabra R, Nagra J (2015): Vaccination of adult animals with a reduced dose of Brucella abortus S19 vaccine to control brucellosis on dairy farms in endemic areas of India. Trop Anim Health Prod, 47, 29-35.
  • 7. Chukwu CC (1986): Comparison of the brucellin skin test with the lymphocyte transformation test in bovine brucellosis. J Hyg. Camb, 96, 403-413.
  • 8. Denoel PA, Vo TK-O, Weynants VE, et al. (1997): Identification of the major T cell antigens present in the Brucella melitensis B115 protein preperation, Brucellergene OCM. J Med Microbiol, 46, 801-806.
  • 9. Dorneles EMS, Sriranganathan N, Lage AP (2015): Recent advances in Brucella abortus vaccines. Vet Res; 46(76), DOI 10.1186/s13567-015-0199-7.
  • 10. Duncombe L, Commander NJ, Erdenliğ S, et al. (2013): Investigating the Use of Protein Saver Cards for Storage and Subsequent Detection of Bovine Anti-Brucella abortus Smooth Lipopolysaccharide Antibodies and Gamma Interferon. Clin Vaccine Immunol, 20(11), 1669 –1674.
  • 11. Duran-Ferrer M, Leon L, Nielsen K, et al. (2004): Antibody response and antigen-specific gamma-interferon profiles of vaccinated and unvaccinated pregnant sheepexperimentally infected withBrucella melitensis.Vet Microbiol, 100, 219-231.
  • 12. Franc KA, Krecek RC, Hasler BN, et al. (2018): Brucellosis remains a neglected disease in the developing world: a call for interdisciplinary action. BMC Public Health, 18 (125), 1-9.
  • 13. Genç O, Büyüktanır Ö, Yurdusev N (2011): Development of an individual rapid test based on enzymatic immunofiltration assay for detection of anti–Brucella abortus antibody in bovine sera. J Vet Diagn Invest, 23, 49– 56.
  • 14. Genç O, Büyüktanır Ö, Serdar G, et al. (2015): Development and validation of sandwich quantitative ELISA prototype based on the bovine IFNg for the detection of cellular immunity. Turk J Vet Anim Sci, 39(6), 724-729.
  • 15. Nielsen K, Smith P, Widdison J, et al. (2004): Serological relationship between cattle exposed to Brucella abortus Yersinia enterocoliticaO:9 and Escherichia coliO157:H7. Vet Microbiol, 100, 25-30.
  • 16. Office International des Epizooties (OIE) (2009): Bovine brucellosis, Section 2.4.3. In OIE Manual of standards for diagnostic tests and vaccines, OIE, Paris, 2009.
  • 17. Perez-Sancho M, Duran-Ferrer M, Garcio-Seco T, et al. (2014): Interferon-gamma responses in sheep exposed to virulent and attenuated Brucella melitensis strains. Vet Immun Immunopathol, 160, 123-128.
  • 18. Plommet M, Fensterbank R(1976):Vaccination against bovine brucellosis with a low dose of strain 19 administered by the conjunctival route. Serological response and immunity in the pregnant cow. Annals Vet Res, 7, 9-23.
  • 19. Stevens MG, Hennager SG, Olsen SC, et al. (1994): Serologic Responses in Diagnostic Tests for Brucellosis in Cattle Vaccinated with Brucella abortus 19 or RB51. J Clin Microbiol, 32(4), 1065-1066.
  • 20. Stevens MG, Pugh GW, Tabatabai LB (1992): Effects of gamma interferon and indomethacin in preventing Brucella abortus infections in mice. Infect. Immun, 60, 4407-4409.
  • 21. Tittarelli M, De Massis F,Bonfini B, et al. (2009): An ELISA for the evaluation of gamma interferon production in cattle vaccinated withBrucella abortusstrain RB51.Vet Ital, 45(2), 347-54.
  • 22. Wareth G, Melzer F, Weise C, et al. (2015): Proteomicsbased identification of immunodominant proteins of Brucella using sera from infected hosts points towards enhanced pathogen survival during the infection. Biochem and Biophys Res Comm, 456, 202-206.
  • 23. Weynants V, Godfroid J, Limbourg B, et al. (1995): Specific bovine brucellosis diagnosis based on in vitro antigen specific gamma interferon production. J Clin Microbiol, 33, 706-712.

Monitoring of the immune response to B. abortus S19 conjunctival vaccine in cattle

Year 2019, Volume: 66 Issue: 1, 53 - 58, 31.12.2018

Oktay Genç , Gülnur Serdar Özlem Büyüktanır Yaş Evrim Genç

Abstract

Brucella abortus S19strain is one of the most preferred strains in vaccines against brucellosis in cattle. However,
monitoring of the B. abortus S19 vaccine is difficult due to non availability of sustainable immunoreactive antigen and accurate test
method. In this study, the humoral and the cellular immune responsesto S19 vaccine in one year old heifers and calves were monitored
on post vaccination days (pvd) of 46, 85 and 169. Thus, the levels of Immunoglobulin (Ig)G and IgA isotypes against lipopolysaccharide
(LPS) for humoral immunity and interferon gamma (IFNg) against brucellergen for cellular immune response were investigated by
home-made ELISAs. In this study, significant IgG positivity was observed on pvd 46 in calves (100%) and heifers (96.6%), but IgA
positivity and IFNg levels were not over 50%. Moreover, percentage of positive animals for IFNg (13.3-43.3%) and IgA (0-44%) have
shown no significance for monitoring the vaccine throughout the study. Therefore, IgG levels can be used to monitor the efficiency of
Brucella abortus S19 conjunctival vaccine in cattle. On the other hand, novel antigen combinations along with brucellergen and LPS
for monitoring the immunity would enhance the sensitivity of the test and could be recommended for future investigations  

Keywords

B. abortus S19 vaccine conjunctival route immunity

References

  • 1. Aragon V, Diaz R, Moreno E, et al. (1996): Characterization of B. abortus and B. melitensis Native Haptens as Outer Membrane O-Type Polysaccharides Independent from the Smooth Lipopolysaccharide. J Bacteriology, 178(4), 1070–1079.
  • 2. Barquero-Calvo E, Chaves-Olarte E, Weiss DS, et al. (2007): B. abortus uses a stealthy strategy to avoid activation of the innate immune system during the onset of infection. PLoS One. 2:e631.
  • 3. Brooks-Worrell BM, Splitter GA (1992):Sodium dodecyl sulfate and salt extracted antigens from various Brucella species induce proliferation of bovine lymphocytes. Infect Immun, 60(5), 2136-2138.
  • 4. Caroff M, Bundle DR, Perry MB, et al. (1984):Antigenic S-type lipopolysaccharide of Brucella abortus 1119-3. Infect Immun; 46, 384-388.
  • 5. Cha S-B, Rayamajhi N, Kang M-L, et al. (2010): Comparative study of gamma interferon production in mice immunized with outer membrane proteins and whole bacteria of Brucella abortus. Jpn J Infect Dis, 63, 49-51.
  • 6. Chand P, Chhabra R, Nagra J (2015): Vaccination of adult animals with a reduced dose of Brucella abortus S19 vaccine to control brucellosis on dairy farms in endemic areas of India. Trop Anim Health Prod, 47, 29-35.
  • 7. Chukwu CC (1986): Comparison of the brucellin skin test with the lymphocyte transformation test in bovine brucellosis. J Hyg. Camb, 96, 403-413.
  • 8. Denoel PA, Vo TK-O, Weynants VE, et al. (1997): Identification of the major T cell antigens present in the Brucella melitensis B115 protein preperation, Brucellergene OCM. J Med Microbiol, 46, 801-806.
  • 9. Dorneles EMS, Sriranganathan N, Lage AP (2015): Recent advances in Brucella abortus vaccines. Vet Res; 46(76), DOI 10.1186/s13567-015-0199-7.
  • 10. Duncombe L, Commander NJ, Erdenliğ S, et al. (2013): Investigating the Use of Protein Saver Cards for Storage and Subsequent Detection of Bovine Anti-Brucella abortus Smooth Lipopolysaccharide Antibodies and Gamma Interferon. Clin Vaccine Immunol, 20(11), 1669 –1674.
  • 11. Duran-Ferrer M, Leon L, Nielsen K, et al. (2004): Antibody response and antigen-specific gamma-interferon profiles of vaccinated and unvaccinated pregnant sheepexperimentally infected withBrucella melitensis.Vet Microbiol, 100, 219-231.
  • 12. Franc KA, Krecek RC, Hasler BN, et al. (2018): Brucellosis remains a neglected disease in the developing world: a call for interdisciplinary action. BMC Public Health, 18 (125), 1-9.
  • 13. Genç O, Büyüktanır Ö, Yurdusev N (2011): Development of an individual rapid test based on enzymatic immunofiltration assay for detection of anti–Brucella abortus antibody in bovine sera. J Vet Diagn Invest, 23, 49– 56.
  • 14. Genç O, Büyüktanır Ö, Serdar G, et al. (2015): Development and validation of sandwich quantitative ELISA prototype based on the bovine IFNg for the detection of cellular immunity. Turk J Vet Anim Sci, 39(6), 724-729.
  • 15. Nielsen K, Smith P, Widdison J, et al. (2004): Serological relationship between cattle exposed to Brucella abortus Yersinia enterocoliticaO:9 and Escherichia coliO157:H7. Vet Microbiol, 100, 25-30.
  • 16. Office International des Epizooties (OIE) (2009): Bovine brucellosis, Section 2.4.3. In OIE Manual of standards for diagnostic tests and vaccines, OIE, Paris, 2009.
  • 17. Perez-Sancho M, Duran-Ferrer M, Garcio-Seco T, et al. (2014): Interferon-gamma responses in sheep exposed to virulent and attenuated Brucella melitensis strains. Vet Immun Immunopathol, 160, 123-128.
  • 18. Plommet M, Fensterbank R(1976):Vaccination against bovine brucellosis with a low dose of strain 19 administered by the conjunctival route. Serological response and immunity in the pregnant cow. Annals Vet Res, 7, 9-23.
  • 19. Stevens MG, Hennager SG, Olsen SC, et al. (1994): Serologic Responses in Diagnostic Tests for Brucellosis in Cattle Vaccinated with Brucella abortus 19 or RB51. J Clin Microbiol, 32(4), 1065-1066.
  • 20. Stevens MG, Pugh GW, Tabatabai LB (1992): Effects of gamma interferon and indomethacin in preventing Brucella abortus infections in mice. Infect. Immun, 60, 4407-4409.
  • 21. Tittarelli M, De Massis F,Bonfini B, et al. (2009): An ELISA for the evaluation of gamma interferon production in cattle vaccinated withBrucella abortusstrain RB51.Vet Ital, 45(2), 347-54.
  • 22. Wareth G, Melzer F, Weise C, et al. (2015): Proteomicsbased identification of immunodominant proteins of Brucella using sera from infected hosts points towards enhanced pathogen survival during the infection. Biochem and Biophys Res Comm, 456, 202-206.
  • 23. Weynants V, Godfroid J, Limbourg B, et al. (1995): Specific bovine brucellosis diagnosis based on in vitro antigen specific gamma interferon production. J Clin Microbiol, 33, 706-712.
There are 23 citations in total.

Details

Primary Language English
Journal Section Research Article
Authors

Oktay Genç

Gülnur Serdar

Özlem Büyüktanır Yaş

Evrim Genç

Publication Date December 31, 2018
Published in Issue Year 2019Volume: 66 Issue: 1

Cite

APA Genç, O., Serdar, G., Büyüktanır Yaş, Ö., Genç, E. (2018). Monitoring of the immune response to B. abortus S19 conjunctival vaccine in cattle. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 66(1), 53-58.
AMA Genç O, Serdar G, Büyüktanır Yaş Ö, Genç E. Monitoring of the immune response to B. abortus S19 conjunctival vaccine in cattle. Ankara Univ Vet Fak Derg. December 2018;66(1):53-58.
Chicago Genç, Oktay, Gülnur Serdar, Özlem Büyüktanır Yaş, and Evrim Genç. “Monitoring of the Immune Response to B. Abortus S19 Conjunctival Vaccine in Cattle”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 66, no. 1 (December 2018): 53-58.
EndNote Genç O, Serdar G, Büyüktanır Yaş Ö, Genç E (December 1, 2018) Monitoring of the immune response to B. abortus S19 conjunctival vaccine in cattle. Ankara Üniversitesi Veteriner Fakültesi Dergisi 66 1 53–58.
IEEE O. Genç, G. Serdar, Ö. Büyüktanır Yaş, and E. Genç, “Monitoring of the immune response to B. abortus S19 conjunctival vaccine in cattle”, Ankara Univ Vet Fak Derg, vol. 66, no. 1, pp. 53–58, 2018.
ISNAD Genç, Oktay et al. “Monitoring of the Immune Response to B. Abortus S19 Conjunctival Vaccine in Cattle”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 66/1 (December 2018), 53-58.
JAMA Genç O, Serdar G, Büyüktanır Yaş Ö, Genç E. Monitoring of the immune response to B. abortus S19 conjunctival vaccine in cattle. Ankara Univ Vet Fak Derg. 2018;66:53–58.
MLA Genç, Oktay et al. “Monitoring of the Immune Response to B. Abortus S19 Conjunctival Vaccine in Cattle”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 66, no. 1, 2018, pp. 53-58.
Vancouver Genç O, Serdar G, Büyüktanır Yaş Ö, Genç E. Monitoring of the immune response to B. abortus S19 conjunctival vaccine in cattle. Ankara Univ Vet Fak Derg. 2018;66(1):53-8.