Research Article
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Year 2019, Volume: 66 Issue: 1, 83 - 87, 31.12.2018

Hatem Atalla Mithat Evecen , Serhat Pabuccuoğlu Sema Birler

Abstract

References

  • 1. Alçay S, Üstüner B, Nur Z (2016): Effects of low molecular weight cryoprotectants on the post-thaw ram sperm quality and fertilizing ability. Small Ruminant Research, 136, 59-64.
  • 2. Ali J, Shelton J (1993): Successful vitrification of day-6 sheep embryos. Journal of J Reprod Fert, 99, 65-70. 3. Bavister BD (1998): Role of oviductal secretions in embryonic growth in vivo and in vitro. Theriogenology, 29, 143-154.
  • 4. Birler S, Pabuccuoğlu S, Alkan S, et al. (2001): Effects of different maturation and culture media on IVF of sheep oocytes. Pakistan Vet J, 100-6 ,102.
  • 5. De Paz P, Sanchez A, Fernandez J, et al. (1994): Sheep embryo cryopreservation by vitrification and conventional freezing. Theriogenology, 42, 327-338.
  • 6. Donnay I, Van Langendonckt A, Auquier P, et al. (1997): Effects of co-culture and embryo number on the in vitro development of bovine embryos. Theriogenology, 47, 1549- 1561.
  • 7. Gandolfi F, Moor R (1987): Stimulation of early embryonic development in the sheep by co-culture with oviduct epithelial cells. J Reprod Fert, 81,23-28.
  • 8. Garcia-Garcia R, Gonzalez-Bulnes A, Dominguez V, et al. (2006): Survival of frozen-thawed sheep embryos cryopreserved at cleavage stages. Cryobiology, 52, 108- 113.
  • 9. Gupta MK, Lee HT (2010): Cryopreservation of oocytes and embryos by vitrification. Korean J Reprod Med, 37, 267-291.
  • 10. Heidari B, Shirazi A, Naderi M, et al. (2013): Effect of various co-culture systems on embryo development in ovine. Czech J Anim Sci, 58, 443-452.
  • 11. Jang H, Jung Y, Cheong H, et al. (2008): Effects of cell status of bovine oviduct epithelial cell (BOEC) on the development of bovine IVM/IVF embryos and gene expression in the BOEC used or not used for the embryo culture. Asian-Aust J Anim Sci, 21, 980-987.
  • 12. Ledda S, Bogliolo L, Leoni G, et al. (1995): Two culture systems showing a biphasic effect on ovine embryo development from the 1-2 cell stage to hatched blastocysts. Reprod Nutr Dev, 35, 629-637.
  • 13. Manokaran S, Veerapandian C, Balasubramanian S (2012): In vitro fertilization of sheep oocytes matured in two different media. Indian J Small Ruminants, 18, 44-46.
  • 14. Martinez A, Matkovic M (1998): Cryopreservation of ovine embryos: slow freezing and vitrification. Theriogenology, 49, 1039-1049.
  • 15. Martínez A, Valcárcel A, Furnus C, et al. (2006): Cryopreservation of in vitro-produced ovine embryos. Small Ruminant Research, 63, 288-296.
  • 16. Nedambale T, Dinnyes A, Groen W, et al. (2004): Comparison on in vitro fertilized bovine embryos cultured in KSOM or SOF and cryopreserved by slow freezing or vitrification. Theriogenology, 62, 437-449.
  • 17. Niemann H (1991): Cryopreservation of ova and embryos from livestock: current status and research needs. Theriogenology, 35, 109-124.
  • 18. Paramio MT, Izquierdo D (2014): Current status of in vitro embryo production in sheep and goats. Reprod Dom Anim, 49, 37- 48.
  • 19. Pereira R, Marques C (2008): Animal oocyte and embryo cryopreservation. Cell and Tissue Banking, 9, 267-277.
  • 20. Riha J, Machatkova M, Pavlok A (2002): Viability of fresh and frozen transferred IVP bovine embryos. Czech J Anim Sci, 47, 261-267.
  • 21. Sakkas D, Trounson A, Kola I (1989): In vivo cleavage rates and viability obtained for early cleavage mouse embryos in co-culture with oviduct cells. Reprod Fer Dev, 1, 127-136.
  • 22. Schiewe M, Rall W, Stuart L, et al. (1991): Analysis of cryoprotectant, cooling rate and in situ dilution using conventional freezing or vitrification for cryopreserving sheep embryos. Theriogenology, 36, 279-293.
  • 23. Shirazi A, Motaghi E (2013): The in vitro fertilization of ovine oocytes in the presence of oviductal cells and its effect on the expression of zygote arrest 1 (zar1) and subsequent embryonic development. J Reprod Infert, 14, 8-16
  • 24. Songsasen N, Buckrell B, Plante C, et al. (1995): In vitro and in vivo survival of cryopreserved sheep embryos. Cryobiology, 32, 78-91.
  • 25. Tominaga K (2004): Cryopreservation and sexing of in vivo-and in vitro-produced bovine embryos for their practical use. Journal of Reproduction and Development, 50, 29-38.
  • 26. Vajta G (2000): Vitrification of the oocytes and embryos of domestic animals. Anim Reprod, 60, 357-364.
  • 27. Van Wagtendonk-de Leeuw A, Mullaart E, De Roos A, et al. (2000): Effects of different reproduction techniques: AI, MOET or IVP, on health and welfare of bovine offspring. Theriogenology, 53, 575-597.
  • 28. Villamil PR, Lozano D, Oviedo J, et al. (2012): Developmental rates of in vivo and in vitro produced bovine embryos cryopreserved in ethylene glycol based solutions by slow freezing or solid surface vitrification. Anim Reprod, 9, 86-92.
  • 29. Yadav P, Saini A, Kumar A, et al. (1998): Effect of oviductal cell co-culture on cleavage and development of goat IVF embryos. Anim Reprod, 51, 301-306.
  • 30. Yang N, Lu K, Gordon I, et al. (1992): Vitrification of bovine blastocysts produced in vitro. Theriogenology, 37, 326 (abstract).
  • 31. Youngs CR (2011): Cryopreservation of preimplantation embryos of cattle, sheep, and goats. J Vis Exp, 54, 2764

Effects of equilibration media and co-culture on vitrification of sheep blastocysts derived in vitro

Year 2019, Volume: 66 Issue: 1, 83 - 87, 31.12.2018

Hatem Atalla Mithat Evecen , Serhat Pabuccuoğlu Sema Birler

Abstract

The effects of two vitrification protocols on the survival of sheep blastocysts were examined in embryos produced
in vitro with sheep oviduct epithelial cells co-culture (CC) or without co-culture (C). Oocytes collected from slaughtered ewes were
matured for 24 h, fertilized with fresh ram semen for 20 h and cultured in SOF medium for up to 9 days in vitro. For vitrification,
blastocyst stage embryos were assigned to two equilibration groups (20% ethylen glycol (EG) or 10% glycerol (G) for the first
equilibration), and as the second equilibration they were kept in 20% ethylen glycol plus 10% glycerol for 5 minutes. After 30 sec in
vitrification solution (25% ethylen glycol + 25% glycerol), they were immediately immersed into liquid nitrogen. After thawing
procedure, embryos were transferred into 0.25 M sucrose for 5 min, washed in Hepes buffered synthetic oviduct fluid (HSOF) and
cultured in SOF medium for 24 h. Cleavage rates were 75.2% in C and 74.2% in CC groups, and blastocyst rates were 14.4% in C and
17.1% in CC groups. After in vitro culture of vitrified-thawed blastocysts, survival rates were 62.1, 38.4, 30.2, and 39.3% in C-EG,
CC-EG, C-G and CC-G groups, respectively. This study shows that vitrification of sheep embryos using ethylene glycol instead of
glycerol as a first equilibration cryoprotectant could give reasonable survival rates and that co-culture of embryos with sheep oviduct
epithelial cell has no beneficial effect on vitrification of embryos.  

Keywords

Co-culture equilibration medium in vitro fertilization sheep vitrification

References

  • 1. Alçay S, Üstüner B, Nur Z (2016): Effects of low molecular weight cryoprotectants on the post-thaw ram sperm quality and fertilizing ability. Small Ruminant Research, 136, 59-64.
  • 2. Ali J, Shelton J (1993): Successful vitrification of day-6 sheep embryos. Journal of J Reprod Fert, 99, 65-70. 3. Bavister BD (1998): Role of oviductal secretions in embryonic growth in vivo and in vitro. Theriogenology, 29, 143-154.
  • 4. Birler S, Pabuccuoğlu S, Alkan S, et al. (2001): Effects of different maturation and culture media on IVF of sheep oocytes. Pakistan Vet J, 100-6 ,102.
  • 5. De Paz P, Sanchez A, Fernandez J, et al. (1994): Sheep embryo cryopreservation by vitrification and conventional freezing. Theriogenology, 42, 327-338.
  • 6. Donnay I, Van Langendonckt A, Auquier P, et al. (1997): Effects of co-culture and embryo number on the in vitro development of bovine embryos. Theriogenology, 47, 1549- 1561.
  • 7. Gandolfi F, Moor R (1987): Stimulation of early embryonic development in the sheep by co-culture with oviduct epithelial cells. J Reprod Fert, 81,23-28.
  • 8. Garcia-Garcia R, Gonzalez-Bulnes A, Dominguez V, et al. (2006): Survival of frozen-thawed sheep embryos cryopreserved at cleavage stages. Cryobiology, 52, 108- 113.
  • 9. Gupta MK, Lee HT (2010): Cryopreservation of oocytes and embryos by vitrification. Korean J Reprod Med, 37, 267-291.
  • 10. Heidari B, Shirazi A, Naderi M, et al. (2013): Effect of various co-culture systems on embryo development in ovine. Czech J Anim Sci, 58, 443-452.
  • 11. Jang H, Jung Y, Cheong H, et al. (2008): Effects of cell status of bovine oviduct epithelial cell (BOEC) on the development of bovine IVM/IVF embryos and gene expression in the BOEC used or not used for the embryo culture. Asian-Aust J Anim Sci, 21, 980-987.
  • 12. Ledda S, Bogliolo L, Leoni G, et al. (1995): Two culture systems showing a biphasic effect on ovine embryo development from the 1-2 cell stage to hatched blastocysts. Reprod Nutr Dev, 35, 629-637.
  • 13. Manokaran S, Veerapandian C, Balasubramanian S (2012): In vitro fertilization of sheep oocytes matured in two different media. Indian J Small Ruminants, 18, 44-46.
  • 14. Martinez A, Matkovic M (1998): Cryopreservation of ovine embryos: slow freezing and vitrification. Theriogenology, 49, 1039-1049.
  • 15. Martínez A, Valcárcel A, Furnus C, et al. (2006): Cryopreservation of in vitro-produced ovine embryos. Small Ruminant Research, 63, 288-296.
  • 16. Nedambale T, Dinnyes A, Groen W, et al. (2004): Comparison on in vitro fertilized bovine embryos cultured in KSOM or SOF and cryopreserved by slow freezing or vitrification. Theriogenology, 62, 437-449.
  • 17. Niemann H (1991): Cryopreservation of ova and embryos from livestock: current status and research needs. Theriogenology, 35, 109-124.
  • 18. Paramio MT, Izquierdo D (2014): Current status of in vitro embryo production in sheep and goats. Reprod Dom Anim, 49, 37- 48.
  • 19. Pereira R, Marques C (2008): Animal oocyte and embryo cryopreservation. Cell and Tissue Banking, 9, 267-277.
  • 20. Riha J, Machatkova M, Pavlok A (2002): Viability of fresh and frozen transferred IVP bovine embryos. Czech J Anim Sci, 47, 261-267.
  • 21. Sakkas D, Trounson A, Kola I (1989): In vivo cleavage rates and viability obtained for early cleavage mouse embryos in co-culture with oviduct cells. Reprod Fer Dev, 1, 127-136.
  • 22. Schiewe M, Rall W, Stuart L, et al. (1991): Analysis of cryoprotectant, cooling rate and in situ dilution using conventional freezing or vitrification for cryopreserving sheep embryos. Theriogenology, 36, 279-293.
  • 23. Shirazi A, Motaghi E (2013): The in vitro fertilization of ovine oocytes in the presence of oviductal cells and its effect on the expression of zygote arrest 1 (zar1) and subsequent embryonic development. J Reprod Infert, 14, 8-16
  • 24. Songsasen N, Buckrell B, Plante C, et al. (1995): In vitro and in vivo survival of cryopreserved sheep embryos. Cryobiology, 32, 78-91.
  • 25. Tominaga K (2004): Cryopreservation and sexing of in vivo-and in vitro-produced bovine embryos for their practical use. Journal of Reproduction and Development, 50, 29-38.
  • 26. Vajta G (2000): Vitrification of the oocytes and embryos of domestic animals. Anim Reprod, 60, 357-364.
  • 27. Van Wagtendonk-de Leeuw A, Mullaart E, De Roos A, et al. (2000): Effects of different reproduction techniques: AI, MOET or IVP, on health and welfare of bovine offspring. Theriogenology, 53, 575-597.
  • 28. Villamil PR, Lozano D, Oviedo J, et al. (2012): Developmental rates of in vivo and in vitro produced bovine embryos cryopreserved in ethylene glycol based solutions by slow freezing or solid surface vitrification. Anim Reprod, 9, 86-92.
  • 29. Yadav P, Saini A, Kumar A, et al. (1998): Effect of oviductal cell co-culture on cleavage and development of goat IVF embryos. Anim Reprod, 51, 301-306.
  • 30. Yang N, Lu K, Gordon I, et al. (1992): Vitrification of bovine blastocysts produced in vitro. Theriogenology, 37, 326 (abstract).
  • 31. Youngs CR (2011): Cryopreservation of preimplantation embryos of cattle, sheep, and goats. J Vis Exp, 54, 2764
There are 30 citations in total.

Details

Primary Language English
Journal Section Research Article
Authors

Hatem Atalla

Mithat Evecen

Serhat Pabuccuoğlu

Sema Birler

Publication Date December 31, 2018
Published in Issue Year 2019Volume: 66 Issue: 1

Cite

APA Atalla, H., Evecen, M., Pabuccuoğlu, S., Birler, S. (2018). Effects of equilibration media and co-culture on vitrification of sheep blastocysts derived in vitro. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 66(1), 83-87.
AMA Atalla H, Evecen M, Pabuccuoğlu S, Birler S. Effects of equilibration media and co-culture on vitrification of sheep blastocysts derived in vitro. Ankara Univ Vet Fak Derg. December 2018;66(1):83-87.
Chicago Atalla, Hatem, Mithat Evecen, Serhat Pabuccuoğlu, and Sema Birler. “Effects of Equilibration Media and Co-Culture on Vitrification of Sheep Blastocysts Derived in Vitro”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 66, no. 1 (December 2018): 83-87.
EndNote Atalla H, Evecen M, Pabuccuoğlu S, Birler S (December 1, 2018) Effects of equilibration media and co-culture on vitrification of sheep blastocysts derived in vitro. Ankara Üniversitesi Veteriner Fakültesi Dergisi 66 1 83–87.
IEEE H. Atalla, M. Evecen, S. Pabuccuoğlu, and S. Birler, “Effects of equilibration media and co-culture on vitrification of sheep blastocysts derived in vitro”, Ankara Univ Vet Fak Derg, vol. 66, no. 1, pp. 83–87, 2018.
ISNAD Atalla, Hatem et al. “Effects of Equilibration Media and Co-Culture on Vitrification of Sheep Blastocysts Derived in Vitro”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 66/1 (December 2018), 83-87.
JAMA Atalla H, Evecen M, Pabuccuoğlu S, Birler S. Effects of equilibration media and co-culture on vitrification of sheep blastocysts derived in vitro. Ankara Univ Vet Fak Derg. 2018;66:83–87.
MLA Atalla, Hatem et al. “Effects of Equilibration Media and Co-Culture on Vitrification of Sheep Blastocysts Derived in Vitro”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 66, no. 1, 2018, pp. 83-87.
Vancouver Atalla H, Evecen M, Pabuccuoğlu S, Birler S. Effects of equilibration media and co-culture on vitrification of sheep blastocysts derived in vitro. Ankara Univ Vet Fak Derg. 2018;66(1):83-7.