Research Article
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Non enzymatic isolation of adipose tissue and stromal vascular fraction derived cells

Year 2020, Volume: 67 Issue: 3, 295 - 301, 03.06.2020

Ece Çerçi Hatice Erdost

https://doi.org/10.33988/auvfd.660139

Abstract

The therapeutic potential of the adipose tissue is also supported by a source of mesenchymal stem cells (MSCs), endothelial progenitor cells, mast cells, T- lymphocyte, B lymphocyte, and adipose-resident macrophages with repairing and regenerative ability. The purpose of this study was to compare adipose tissue derived mesenchymal stem cells (ADSCs) and stromal vascular fraction cells (SVF cells) in terms of the usage of non-enzymatic isolation, detection of cell adhesion, fibroblastoid cell formation, properties of cell population, cell culture duration until 3rd passages under in-vitro culture condition. We used 3 months old, 4 male Sprague Dawley rats (mean of live weight about 250 g) to examined the variation of the cell population, cell properties, phases of cellular formation, cell culturing time, subculturing duration, differences in confluency between SVF cells and ADSCs. In in-vitro expansion, ADSCs displayed higher rate of adhesiveness, homogenous cell population, faster proliferation and formation of fibroblast like cells compared to SVF cells. As a result, we showed that ADSCs have better adhesive ability, higher proliferative capacity in all of the 3 passages and require shorter time to reach confluency compared with SVF cells in vitro. These findings may contribute to future studies that deal with isolation and selection of stem cells from various tissues, as well as design clinical trials based on ADSCs and SVF cells.

Keywords

Adipose tissue derived cells mesenchymal stem cells stromal vasculer fraction cells

References

  • 1. Agostini F, Rossi FM, Aldinucci D, et al (2018): Improved GMP compliant approach to manipulate lipoaspirates, to cryopreserve stromal vascular fraction, and to expand adipose stem cells in xeno-free media. Stem Cell Res Ther, 9, 130.
  • 2. Aguena M, Dalto Fanganiello R, Tissiani LAL, et al (2012): Optimization of parameters for a more efficient use of adipose-derived stem cells in regenerative medicine therapies. Stem Cells Int, 303610.
  • 3. Aronowitz JA, Lockhart RA, Hakakian CS (2015): Mechanical versus enzymatic isolation of stromal vascular fraction cells from adipose tissue. Springerplus, 23, 713.
  • 4. Asumda FZ, Chase PB (2011): Age-related changes in rat bone-marrow mesenchymal stem cell plasticity. BMC Cell Biol, 12, 44.
  • 5. Boquest AC, Shahdadfar A, Fronsdal K et al (2005): Isolation and transcription profiling of purified uncultured human stromal stem cells: alteration of gene expression after in vitro cell culture. Mol Biol Cell, 16, 1131-1141.
  • 6. Bongso A, Fong C (2009): Human Embryonic Stem Cells: Their Nature, Properties, and Uses. 1-18 In: H Baharvand (Ed). Trends in Stem Cell Biology and Technology. Humana Press, New York.
  • 7. Chazenbalk G1, Bertolotto C, Heneidi S, et al (2011): Novel Pathway of Adipogenesis through Cross-Talk between Adipose Tissue Macrophages, Adipose Stem Cells and Adipocytes: Evidence of Cell Plasticity. PLoS One, 6, e17834.
  • 8. Condé Green A, Kotamarti VS, Sherman LS, et al (2016): Shift toward Mechanical Isolation of Adipose-derived Stromal Vascular Fraction: Review of Upcoming Techniques. Plastic and reconstructive surgery. Plast Reconstr Surg Glob Open, 4, e1017.
  • 9. Csaki C, Matis U, Mobasheri A, et al (2007): Chondrogenesis, osteogenesis and adipogenesis of canine mesenchymal stem cells: a biochemical, morphological and ultrastructural study. Histochem Cell Biol, 128, 507-520.
  • 10. Ghorbani A, Jalali SA, Varedi M (2014): Isolation of adipose tissue mesenchymal stem cells without tissue destruction: a non-enzymatic method. Tissue Cell, 46, 54-58.
  • 11. Gimble JM, Guilak F (2003): Differentiation potential of adipose derived adult stem (ADAS) cells. Curr Top Dev Biol, 58, 137-160.
  • 12. Gimble JM (2003): Adipose tissue-derived therapeutics. Expert Opin Biol Ther, 3, 705-713.
  • 13. Katsara O, Mahaira LG, Iliopoulou EG, et al (2011): Effects of donor age, gender, and in vitro cellular aging on the phenotypic, functional, and molecular characteristics of mouse bone marrow-derived mesenchymal stem cells. Stem Cells Dev, 20, 1549-1561.
  • 14. López C, Ajenjo N, Muñoz-Alonso MJ, et al (2008): Determination of viability of human cartilage allografts by a rapid and quantitative method not requiring cartilage digestion. Cell Transplant, 17, 859-864.
  • 15. Mildmay-White A, Khan W. (2009): Cell surface Markers on Adipose-Derived Stem Cells: A Systematic Review. Curr Stem Cell Res Ther, 12, 484-492.
  • 16. Nadri S, Soleimani M (2007): Isolation murine mesenchymal stem cells by positive selection. In Vitro Cell Dev Biol Anim, 43, 276-282.
  • 17. Peng L, Jia Z, Yin X, et al (2008):Comparative analysis of mesenchymal stem cells from bone marrow, cartilage, and adipose tissue. Stem Cells Dev, 17, 761-773. 18. Rodbell, M (1964): The metabolism of isolated fat cells. Compr Physiol, 47, 471-482.
  • 19. Sancak IG, Özen A, Bayraktaroğlu AG, Ceylan A , Can P. (2016): Characterization of mesenchymal stem cells isolated from the adipose tissue of young and old dogs. Ankara Univ Vet Fak Derg, 63, 297-302.
  • 20. Satija NK, Singh VK, Verma YK, et al (2009): Mesenchymal stem cell-based therapy: a new paradigm in regenerative medicine. J Cell Mol Med, 13, 4385-4402.
  • 21. Sarraf CE, Otto WR, Eastwood M (2011): In vitro mesenchymal stem cell differentiation after mechanical stimulation. Cell Prolif, 44, 99-108.
  • 22. Shah FS, Wu X, Dietrich M, et al (2013): A non-enzymatic method for isolating human adipose tissue-derived stromal stem cells. Cytotherapy, 15, 979-985.
  • 23. Sun CK, Yen CH, Lin YC, et al (2011): Autologous transplantation of adipose-derived mesenchymal stem cells markedly reduced acute ischemia-reperfusion lung injury in a rodent model. J Transl Med, 9, 118.
  • 24. Strioga M, Viswanathan S, Darinskas A, et al (2012): Same or not the same? Comparison of adipose tissue-derived versus bone marrow-derived mesenchymal stem and stromal cells. Stem Cells Dev, 21, 2724-2752.
  • 25. Thirumala S, Gimble JM, Devireddy RV (2010): Cryopreservation of stromal vascular fraction of adipose tissue in a serum-free freezing medium. J Tissue Eng Regen Med, 4, 224-232.
  • 26. Van Dijk A, Naaijkens BA, Jurgens WJFM, et al (2011): Reduction of infarct size by intravenous injection of uncultured adipose derived stromal cells in a rat model is dependent on the time point of application. Stem Cell Res, 7, 219-229.
  • 27. Varma MJ, Breuls RG, Schouten TE, et al (2007): Phenotypical and functional characterization of freshly isolated adipose tissue-derived stem cells. Stem Cells Dev. 16, 91-104.
  • 28. You D, Jang MJ, Kim BH, et al (2015): Comparative study of autologous stromal vascular fraction and adipose-derived stem cells for erectile function recovery in a rat model of cavernous nerve injury. Stem Cells Transl Med, 4, 351-358.
  • 29. Zhu Y, Liu T, Song K, et al (2008): Adipose-derived stem cell: a better stem cell than BMSC. Cell Biochem Funct, 26, 664-675.
  • 30. Zuk PA, Zhu M, Mizuno H, et al (2001): Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng, 7, 211-28.

Year 2020, Volume: 67 Issue: 3, 295 - 301, 03.06.2020

Ece Çerçi Hatice Erdost

https://doi.org/10.33988/auvfd.660139

Abstract

References

  • 1. Agostini F, Rossi FM, Aldinucci D, et al (2018): Improved GMP compliant approach to manipulate lipoaspirates, to cryopreserve stromal vascular fraction, and to expand adipose stem cells in xeno-free media. Stem Cell Res Ther, 9, 130.
  • 2. Aguena M, Dalto Fanganiello R, Tissiani LAL, et al (2012): Optimization of parameters for a more efficient use of adipose-derived stem cells in regenerative medicine therapies. Stem Cells Int, 303610.
  • 3. Aronowitz JA, Lockhart RA, Hakakian CS (2015): Mechanical versus enzymatic isolation of stromal vascular fraction cells from adipose tissue. Springerplus, 23, 713.
  • 4. Asumda FZ, Chase PB (2011): Age-related changes in rat bone-marrow mesenchymal stem cell plasticity. BMC Cell Biol, 12, 44.
  • 5. Boquest AC, Shahdadfar A, Fronsdal K et al (2005): Isolation and transcription profiling of purified uncultured human stromal stem cells: alteration of gene expression after in vitro cell culture. Mol Biol Cell, 16, 1131-1141.
  • 6. Bongso A, Fong C (2009): Human Embryonic Stem Cells: Their Nature, Properties, and Uses. 1-18 In: H Baharvand (Ed). Trends in Stem Cell Biology and Technology. Humana Press, New York.
  • 7. Chazenbalk G1, Bertolotto C, Heneidi S, et al (2011): Novel Pathway of Adipogenesis through Cross-Talk between Adipose Tissue Macrophages, Adipose Stem Cells and Adipocytes: Evidence of Cell Plasticity. PLoS One, 6, e17834.
  • 8. Condé Green A, Kotamarti VS, Sherman LS, et al (2016): Shift toward Mechanical Isolation of Adipose-derived Stromal Vascular Fraction: Review of Upcoming Techniques. Plastic and reconstructive surgery. Plast Reconstr Surg Glob Open, 4, e1017.
  • 9. Csaki C, Matis U, Mobasheri A, et al (2007): Chondrogenesis, osteogenesis and adipogenesis of canine mesenchymal stem cells: a biochemical, morphological and ultrastructural study. Histochem Cell Biol, 128, 507-520.
  • 10. Ghorbani A, Jalali SA, Varedi M (2014): Isolation of adipose tissue mesenchymal stem cells without tissue destruction: a non-enzymatic method. Tissue Cell, 46, 54-58.
  • 11. Gimble JM, Guilak F (2003): Differentiation potential of adipose derived adult stem (ADAS) cells. Curr Top Dev Biol, 58, 137-160.
  • 12. Gimble JM (2003): Adipose tissue-derived therapeutics. Expert Opin Biol Ther, 3, 705-713.
  • 13. Katsara O, Mahaira LG, Iliopoulou EG, et al (2011): Effects of donor age, gender, and in vitro cellular aging on the phenotypic, functional, and molecular characteristics of mouse bone marrow-derived mesenchymal stem cells. Stem Cells Dev, 20, 1549-1561.
  • 14. López C, Ajenjo N, Muñoz-Alonso MJ, et al (2008): Determination of viability of human cartilage allografts by a rapid and quantitative method not requiring cartilage digestion. Cell Transplant, 17, 859-864.
  • 15. Mildmay-White A, Khan W. (2009): Cell surface Markers on Adipose-Derived Stem Cells: A Systematic Review. Curr Stem Cell Res Ther, 12, 484-492.
  • 16. Nadri S, Soleimani M (2007): Isolation murine mesenchymal stem cells by positive selection. In Vitro Cell Dev Biol Anim, 43, 276-282.
  • 17. Peng L, Jia Z, Yin X, et al (2008):Comparative analysis of mesenchymal stem cells from bone marrow, cartilage, and adipose tissue. Stem Cells Dev, 17, 761-773. 18. Rodbell, M (1964): The metabolism of isolated fat cells. Compr Physiol, 47, 471-482.
  • 19. Sancak IG, Özen A, Bayraktaroğlu AG, Ceylan A , Can P. (2016): Characterization of mesenchymal stem cells isolated from the adipose tissue of young and old dogs. Ankara Univ Vet Fak Derg, 63, 297-302.
  • 20. Satija NK, Singh VK, Verma YK, et al (2009): Mesenchymal stem cell-based therapy: a new paradigm in regenerative medicine. J Cell Mol Med, 13, 4385-4402.
  • 21. Sarraf CE, Otto WR, Eastwood M (2011): In vitro mesenchymal stem cell differentiation after mechanical stimulation. Cell Prolif, 44, 99-108.
  • 22. Shah FS, Wu X, Dietrich M, et al (2013): A non-enzymatic method for isolating human adipose tissue-derived stromal stem cells. Cytotherapy, 15, 979-985.
  • 23. Sun CK, Yen CH, Lin YC, et al (2011): Autologous transplantation of adipose-derived mesenchymal stem cells markedly reduced acute ischemia-reperfusion lung injury in a rodent model. J Transl Med, 9, 118.
  • 24. Strioga M, Viswanathan S, Darinskas A, et al (2012): Same or not the same? Comparison of adipose tissue-derived versus bone marrow-derived mesenchymal stem and stromal cells. Stem Cells Dev, 21, 2724-2752.
  • 25. Thirumala S, Gimble JM, Devireddy RV (2010): Cryopreservation of stromal vascular fraction of adipose tissue in a serum-free freezing medium. J Tissue Eng Regen Med, 4, 224-232.
  • 26. Van Dijk A, Naaijkens BA, Jurgens WJFM, et al (2011): Reduction of infarct size by intravenous injection of uncultured adipose derived stromal cells in a rat model is dependent on the time point of application. Stem Cell Res, 7, 219-229.
  • 27. Varma MJ, Breuls RG, Schouten TE, et al (2007): Phenotypical and functional characterization of freshly isolated adipose tissue-derived stem cells. Stem Cells Dev. 16, 91-104.
  • 28. You D, Jang MJ, Kim BH, et al (2015): Comparative study of autologous stromal vascular fraction and adipose-derived stem cells for erectile function recovery in a rat model of cavernous nerve injury. Stem Cells Transl Med, 4, 351-358.
  • 29. Zhu Y, Liu T, Song K, et al (2008): Adipose-derived stem cell: a better stem cell than BMSC. Cell Biochem Funct, 26, 664-675.
  • 30. Zuk PA, Zhu M, Mizuno H, et al (2001): Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng, 7, 211-28.
There are 29 citations in total.

Details

Primary Language English
Subjects Veterinary Surgery
Journal Section Research Article
Authors

Ece Çerçi 0000-0002-2740-4262

Hatice Erdost 0000-0003-1547-7293

Publication Date June 3, 2020
Published in Issue Year 2020Volume: 67 Issue: 3

Cite

APA Çerçi, E., & Erdost, H. (2020). Non enzymatic isolation of adipose tissue and stromal vascular fraction derived cells. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 67(3), 295-301. https://doi.org/10.33988/auvfd.660139
AMA Çerçi E, Erdost H. Non enzymatic isolation of adipose tissue and stromal vascular fraction derived cells. Ankara Univ Vet Fak Derg. June 2020;67(3):295-301. doi:10.33988/auvfd.660139
Chicago Çerçi, Ece, and Hatice Erdost. “Non Enzymatic Isolation of Adipose Tissue and Stromal Vascular Fraction Derived Cells”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 67, no. 3 (June 2020): 295-301. https://doi.org/10.33988/auvfd.660139.
EndNote Çerçi E, Erdost H (June 1, 2020) Non enzymatic isolation of adipose tissue and stromal vascular fraction derived cells. Ankara Üniversitesi Veteriner Fakültesi Dergisi 67 3 295–301.
IEEE E. Çerçi and H. Erdost, “Non enzymatic isolation of adipose tissue and stromal vascular fraction derived cells”, Ankara Univ Vet Fak Derg, vol. 67, no. 3, pp. 295–301, 2020, doi: 10.33988/auvfd.660139.
ISNAD Çerçi, Ece - Erdost, Hatice. “Non Enzymatic Isolation of Adipose Tissue and Stromal Vascular Fraction Derived Cells”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 67/3 (June 2020), 295-301. https://doi.org/10.33988/auvfd.660139.
JAMA Çerçi E, Erdost H. Non enzymatic isolation of adipose tissue and stromal vascular fraction derived cells. Ankara Univ Vet Fak Derg. 2020;67:295–301.
MLA Çerçi, Ece and Hatice Erdost. “Non Enzymatic Isolation of Adipose Tissue and Stromal Vascular Fraction Derived Cells”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 67, no. 3, 2020, pp. 295-01, doi:10.33988/auvfd.660139.
Vancouver Çerçi E, Erdost H. Non enzymatic isolation of adipose tissue and stromal vascular fraction derived cells. Ankara Univ Vet Fak Derg. 2020;67(3):295-301.