Year 2020, Volume 67 , Issue 4, Pages 365 - 371 2020-09-01

Determination of virulence factors and antimicrobial resistance of E. coli isolated from calf diarrhea, part of eastern Turkey

Seyda CENGİZ [1] , Mehmet Cemal ADIGÜZEL [2]


Microorganisms have a primary role in the formation of calf diarrhea. Escherichia coli pose an environmental risk to young animals caused by fecal excretion. In this study, rectal swab samples (n= 133) were collected from calves with diarrhea aged from 1 day to 3 months, between August 2017 and August 2018. The samples were cultured on MacConkey agar, and then antimicrobial susceptibility and virulence genes for Escherichia coli isolates (n= 133) were investigated by disk diffusion method according to clinical and laboratory standards institute standards and multiplex polymerase chain reaction, respectively. The isolates were found to be highly resistant to oxytetracycline (78.9%), trimethoprim-sulfamethoxazole (69.2%), neomycin (60.9%), and erythromycin (58.6%). Besides, multidrug resistance was determined in 71.4% of isolates. Thirty-three of 133 (24.81%) isolates were positive for at least one virulence factor. The pathotypes of enterotoxigenic Escherichia coli (F5 and/or F41 fimbria and STa), enterohemorrhagic Escherichia coli (Stx and eae), enteropathogenic Escherichia coli (eae) and Shiga toxin-producing Escherichia coli (Stx-eae) were found in 51.5%, 6.1%, 15.2%, and 12.1%, respectively. However, the virulence properties were detected as; Stx1 (3.03%), Stx2 (9.09%), STa (21.21%), and eae (15.15%); the F41 and F5 were not detected. Also, the fifteen-point two percent of strains (5/33) were the hybrid type that carried both Stx (either Stx1 or Stx2) and enterotoxigenic Escherichia coli specific enterotoxin gene STa. The existence of different virulence factors found in this study supports the statement that calves are possible bearers of pathogens that are dangerous to public health.

Antimicrobial Resistance, diarrhoea, Escherichia coli, hybrid strain, virulence gene
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Primary Language en
Subjects Veterinary
Journal Section Research Article
Authors

Orcid: 0000-0002-1273-2941
Author: Seyda CENGİZ
Institution: Atatürk University, Faculty of Veterinary Medicine, Department of Microbiology
Country: Turkey


Orcid: 0000-0002-2385-9649
Author: Mehmet Cemal ADIGÜZEL
Institution: Atatürk University, Faculty of Veterinary Medicine, Department of Microbiology
Country: Turkey


Dates

Publication Date : September 1, 2020

Bibtex @research article { auvfd640990, journal = {Ankara Üniversitesi Veteriner Fakültesi Dergisi}, issn = {}, eissn = {1308-2817}, address = {}, publisher = {Ankara University}, year = {2020}, volume = {67}, pages = {365 - 371}, doi = {10.33988/auvfd.640990}, title = {Determination of virulence factors and antimicrobial resistance of E. coli isolated from calf diarrhea, part of eastern Turkey}, key = {cite}, author = {Cengi̇z, Seyda and Adıgüzel, Mehmet Cemal} }
APA Cengi̇z, S , Adıgüzel, M . (2020). Determination of virulence factors and antimicrobial resistance of E. coli isolated from calf diarrhea, part of eastern Turkey . Ankara Üniversitesi Veteriner Fakültesi Dergisi , 67 (4) , 365-371 . DOI: 10.33988/auvfd.640990
MLA Cengi̇z, S , Adıgüzel, M . "Determination of virulence factors and antimicrobial resistance of E. coli isolated from calf diarrhea, part of eastern Turkey" . Ankara Üniversitesi Veteriner Fakültesi Dergisi 67 (2020 ): 365-371 <http://vetjournal.ankara.edu.tr/en/pub/issue/56621/640990>
Chicago Cengi̇z, S , Adıgüzel, M . "Determination of virulence factors and antimicrobial resistance of E. coli isolated from calf diarrhea, part of eastern Turkey". Ankara Üniversitesi Veteriner Fakültesi Dergisi 67 (2020 ): 365-371
RIS TY - JOUR T1 - Determination of virulence factors and antimicrobial resistance of E. coli isolated from calf diarrhea, part of eastern Turkey AU - Seyda Cengi̇z , Mehmet Cemal Adıgüzel Y1 - 2020 PY - 2020 N1 - doi: 10.33988/auvfd.640990 DO - 10.33988/auvfd.640990 T2 - Ankara Üniversitesi Veteriner Fakültesi Dergisi JF - Journal JO - JOR SP - 365 EP - 371 VL - 67 IS - 4 SN - -1308-2817 M3 - doi: 10.33988/auvfd.640990 UR - https://doi.org/10.33988/auvfd.640990 Y2 - 2020 ER -
EndNote %0 Ankara Üniversitesi Veteriner Fakültesi Dergisi Determination of virulence factors and antimicrobial resistance of E. coli isolated from calf diarrhea, part of eastern Turkey %A Seyda Cengi̇z , Mehmet Cemal Adıgüzel %T Determination of virulence factors and antimicrobial resistance of E. coli isolated from calf diarrhea, part of eastern Turkey %D 2020 %J Ankara Üniversitesi Veteriner Fakültesi Dergisi %P -1308-2817 %V 67 %N 4 %R doi: 10.33988/auvfd.640990 %U 10.33988/auvfd.640990
ISNAD Cengi̇z, Seyda , Adıgüzel, Mehmet Cemal . "Determination of virulence factors and antimicrobial resistance of E. coli isolated from calf diarrhea, part of eastern Turkey". Ankara Üniversitesi Veteriner Fakültesi Dergisi 67 / 4 (September 2020): 365-371 . https://doi.org/10.33988/auvfd.640990
AMA Cengi̇z S , Adıgüzel M . Determination of virulence factors and antimicrobial resistance of E. coli isolated from calf diarrhea, part of eastern Turkey. Ankara Univ Vet Fak Derg. 2020; 67(4): 365-371.
Vancouver Cengi̇z S , Adıgüzel M . Determination of virulence factors and antimicrobial resistance of E. coli isolated from calf diarrhea, part of eastern Turkey. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2020; 67(4): 365-371.
IEEE S. Cengi̇z and M. Adıgüzel , "Determination of virulence factors and antimicrobial resistance of E. coli isolated from calf diarrhea, part of eastern Turkey", Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 67, no. 4, pp. 365-371, Sep. 2020, doi:10.33988/auvfd.640990