Year 2020, Volume 67 , Issue 4, Pages 399 - 406 2020-09-01

Evaluation of melamine and cyanuric acid cytotoxicity: an in vitro study on L929 fibroblasts and CHO cell line

Abdullah MELEKOĞLU [1] , Hüsamettin EKİCİ [2] , Esra ARAT [3] , Siyami KARAHAN [4]


Melamine and its metabolites pose health concern as they are used in various industrial products including feed and drugs. There are a limited number of studies on melamine and cyanuric acid cytotoxicity and cellular damage without a certain conclusion. The present study aimed to evaluate melamine, cyanuric acid and its combined cytotoxic effects using 3-(4.5-dimethylthiazol-2-yl) methyl thiazole tetrazolium (MTT) bromide test. The study also evaluated apoptotic and necrotic effect using a double staining method of Hoechst 33342 and propidium iodide. Melamine, cyanuric acid and their combination (1:1) were applied to L929 fibroblasts and Chinese hamster ovary (CHO) cells at various concentrations (1000 µg/mL, 500 µg/mL, 250 µg/mL, 125 µg/mL and 62.5 µg/mL). At the highest concentration (1000 µg/mL), the cell viability dropped down approximately to 50% both in CHO cells and L929 cells. Melamine, cyanuric acid and their mixture caused cytotoxicity in CHO cells and L929 fibroblasts in dose-dependent manner. Cell death occurred through both apoptosis and mainly necrosis. Both cell types were more sensitive to the mixture of melamine and cyanuric acid and, furthermore, CHO cells were more sensitive than L929 fibroblasts. As a result, melamine, cyanuric acid and their combination caused cytotoxicity in CHO cells and L929 fibroblasts. Further studies should be conducted in different cell lines. These studies should also aim to reveal the mechanism of cytotoxicity and related pathways. 
Apoptosis, cyanuric acid, cytotoxicity, melamine, necrosis
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Primary Language en
Subjects Veterinary
Journal Section Research Article
Authors

Orcid: 0000-0002-5214-292X
Author: Abdullah MELEKOĞLU
Institution: Kastamonu University, Department of Genetics and Bioengineering
Country: Turkey


Orcid: 0000-0001-6403-737X
Author: Hüsamettin EKİCİ (Primary Author)
Institution: Kırıkkale University, Faculty of Veterinary Medicine, Department of Pharmacology and Toxicology
Country: Turkey


Orcid: 0000-0002-9259-2538
Author: Esra ARAT
Institution: Kırıkkale University Scientific and Technological Research Application and Research Center
Country: Turkey


Orcid: 0000-0002-2744-1717
Author: Siyami KARAHAN
Institution: Kırıkkale University, Faculty of Veterinary Medicine, Department of Histology and Embryology
Country: Turkey


Dates

Publication Date : September 1, 2020

Bibtex @research article { auvfd664059, journal = {Ankara Üniversitesi Veteriner Fakültesi Dergisi}, issn = {}, eissn = {1308-2817}, address = {}, publisher = {Ankara University}, year = {2020}, volume = {67}, pages = {399 - 406}, doi = {10.33988/auvfd.664059}, title = {Evaluation of melamine and cyanuric acid cytotoxicity: an in vitro study on L929 fibroblasts and CHO cell line}, key = {cite}, author = {Melekoğlu, Abdullah and Eki̇ci̇, Hüsamettin and Arat, Esra and Karahan, Siyami} }
APA Melekoğlu, A , Eki̇ci̇, H , Arat, E , Karahan, S . (2020). Evaluation of melamine and cyanuric acid cytotoxicity: an in vitro study on L929 fibroblasts and CHO cell line . Ankara Üniversitesi Veteriner Fakültesi Dergisi , 67 (4) , 399-406 . DOI: 10.33988/auvfd.664059
MLA Melekoğlu, A , Eki̇ci̇, H , Arat, E , Karahan, S . "Evaluation of melamine and cyanuric acid cytotoxicity: an in vitro study on L929 fibroblasts and CHO cell line" . Ankara Üniversitesi Veteriner Fakültesi Dergisi 67 (2020 ): 399-406 <http://vetjournal.ankara.edu.tr/en/pub/issue/56621/664059>
Chicago Melekoğlu, A , Eki̇ci̇, H , Arat, E , Karahan, S . "Evaluation of melamine and cyanuric acid cytotoxicity: an in vitro study on L929 fibroblasts and CHO cell line". Ankara Üniversitesi Veteriner Fakültesi Dergisi 67 (2020 ): 399-406
RIS TY - JOUR T1 - Evaluation of melamine and cyanuric acid cytotoxicity: an in vitro study on L929 fibroblasts and CHO cell line AU - Abdullah Melekoğlu , Hüsamettin Eki̇ci̇ , Esra Arat , Siyami Karahan Y1 - 2020 PY - 2020 N1 - doi: 10.33988/auvfd.664059 DO - 10.33988/auvfd.664059 T2 - Ankara Üniversitesi Veteriner Fakültesi Dergisi JF - Journal JO - JOR SP - 399 EP - 406 VL - 67 IS - 4 SN - -1308-2817 M3 - doi: 10.33988/auvfd.664059 UR - https://doi.org/10.33988/auvfd.664059 Y2 - 2020 ER -
EndNote %0 Ankara Üniversitesi Veteriner Fakültesi Dergisi Evaluation of melamine and cyanuric acid cytotoxicity: an in vitro study on L929 fibroblasts and CHO cell line %A Abdullah Melekoğlu , Hüsamettin Eki̇ci̇ , Esra Arat , Siyami Karahan %T Evaluation of melamine and cyanuric acid cytotoxicity: an in vitro study on L929 fibroblasts and CHO cell line %D 2020 %J Ankara Üniversitesi Veteriner Fakültesi Dergisi %P -1308-2817 %V 67 %N 4 %R doi: 10.33988/auvfd.664059 %U 10.33988/auvfd.664059
ISNAD Melekoğlu, Abdullah , Eki̇ci̇, Hüsamettin , Arat, Esra , Karahan, Siyami . "Evaluation of melamine and cyanuric acid cytotoxicity: an in vitro study on L929 fibroblasts and CHO cell line". Ankara Üniversitesi Veteriner Fakültesi Dergisi 67 / 4 (September 2020): 399-406 . https://doi.org/10.33988/auvfd.664059
AMA Melekoğlu A , Eki̇ci̇ H , Arat E , Karahan S . Evaluation of melamine and cyanuric acid cytotoxicity: an in vitro study on L929 fibroblasts and CHO cell line. Ankara Univ Vet Fak Derg. 2020; 67(4): 399-406.
Vancouver Melekoğlu A , Eki̇ci̇ H , Arat E , Karahan S . Evaluation of melamine and cyanuric acid cytotoxicity: an in vitro study on L929 fibroblasts and CHO cell line. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2020; 67(4): 399-406.
IEEE A. Melekoğlu , H. Eki̇ci̇ , E. Arat and S. Karahan , "Evaluation of melamine and cyanuric acid cytotoxicity: an in vitro study on L929 fibroblasts and CHO cell line", Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 67, no. 4, pp. 399-406, Sep. 2020, doi:10.33988/auvfd.664059