Year 2020, Volume 67 , Issue 4, Pages 407 - 412 2020-09-01

Pilot study on cardiogenic differentiation capability of rabbit mesenchymal stem cells

Natalia GRIGOROVA [1] , Elen GÓCZA [2] , Ekaterina VACHKOVA [3]


Cardiovascular diseases are still one of the most common reasons for mortality in humans. Mesenchymal stem cells (MSCs) are preferable in cardiac regeneration cell-based therapies because of their allogeneic and high proliferative potential. The electrophysiological properties of the rabbit heard is closer to human than the mouse. The current study aimed to trace mRNA expression changes of two stemness/cardiogenic differentiation ability-related transcriptionala factors OCT4 and GATA4 in rabbit MSCs during early stages of induced cardiomyocyte differentiation in vitro. The mesenchymal stem cell originated from different anatomical areas-subcutaneous, visceral, bone marrow and pericardial tissue. The cardiac differentiation protocol for mouse embryonic stem cells in hanging drop was adopted in rabbit MSCs. The best formed embryonal bodies (EBs) like structures were collected and cultivated on gelatin-coated plates. The total mRNA was obtained before cardiac differentiation and on the 6th day after it. SYBER based real-time PCR was performed to evaluate the mRNA expression fold-changes of OCT4 and GATA4. The cultivation of MSCs in hanging drops during cardiac differentiation induced EBs formation, without any contractile activity up to the 6th day of the differentiation in all cell types. The applied differentiation protocol significantly downregulated GATA4 expression in ADSCs - EBs, while in BMSCs, both target genes were significantly upregulated. In conclusion, the adopted cardiac differentiation protocol from mouse embryonic stem cells could be a useful approach for rabbit bone marrow mesenchymal stem cells. Since the rest of the cells revealed weak cardiogenic capability at this early stage, some modifications of induction protocols should be considered.
Cardiogenic differentiation, mesenchymal stem cells, rabbit
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Primary Language en
Subjects Veterinary
Journal Section Research Article
Authors

Orcid: 0000-0001-5749-7773
Author: Natalia GRIGOROVA
Institution: Trakia University, Faculty of Veterinary Medicine, Department of Pharmacology, Animal Physiology and Physiological Chemistry, Stara Zagora
Country: Bulgaria


Orcid: 0000-0001-7720-4720
Author: Elen GÓCZA
Institution: Applied Embryology and Stem Cell Research Group, Department of Animal Biotechnology, Gödöllö
Country: Hungary


Orcid: 0000-0002-9291-332X
Author: Ekaterina VACHKOVA (Primary Author)
Institution: Trakia University, Faculty of Veterinary Medicine, Department of Pharmacology, Animal Physiology and Physiological Chemistry, Stara Zagora
Country: Bulgaria


Dates

Publication Date : September 1, 2020

Bibtex @research article { auvfd682682, journal = {Ankara Üniversitesi Veteriner Fakültesi Dergisi}, issn = {}, eissn = {1308-2817}, address = {}, publisher = {Ankara University}, year = {2020}, volume = {67}, pages = {407 - 412}, doi = {10.33988/auvfd.682682}, title = {Pilot study on cardiogenic differentiation capability of rabbit mesenchymal stem cells}, key = {cite}, author = {Grıgorova, Natalia and Gócza, Elen and Vachkova, Ekaterina} }
APA Grıgorova, N , Gócza, E , Vachkova, E . (2020). Pilot study on cardiogenic differentiation capability of rabbit mesenchymal stem cells . Ankara Üniversitesi Veteriner Fakültesi Dergisi , 67 (4) , 407-412 . DOI: 10.33988/auvfd.682682
MLA Grıgorova, N , Gócza, E , Vachkova, E . "Pilot study on cardiogenic differentiation capability of rabbit mesenchymal stem cells" . Ankara Üniversitesi Veteriner Fakültesi Dergisi 67 (2020 ): 407-412 <http://vetjournal.ankara.edu.tr/en/pub/issue/56621/682682>
Chicago Grıgorova, N , Gócza, E , Vachkova, E . "Pilot study on cardiogenic differentiation capability of rabbit mesenchymal stem cells". Ankara Üniversitesi Veteriner Fakültesi Dergisi 67 (2020 ): 407-412
RIS TY - JOUR T1 - Pilot study on cardiogenic differentiation capability of rabbit mesenchymal stem cells AU - Natalia Grıgorova , Elen Gócza , Ekaterina Vachkova Y1 - 2020 PY - 2020 N1 - doi: 10.33988/auvfd.682682 DO - 10.33988/auvfd.682682 T2 - Ankara Üniversitesi Veteriner Fakültesi Dergisi JF - Journal JO - JOR SP - 407 EP - 412 VL - 67 IS - 4 SN - -1308-2817 M3 - doi: 10.33988/auvfd.682682 UR - https://doi.org/10.33988/auvfd.682682 Y2 - 2020 ER -
EndNote %0 Ankara Üniversitesi Veteriner Fakültesi Dergisi Pilot study on cardiogenic differentiation capability of rabbit mesenchymal stem cells %A Natalia Grıgorova , Elen Gócza , Ekaterina Vachkova %T Pilot study on cardiogenic differentiation capability of rabbit mesenchymal stem cells %D 2020 %J Ankara Üniversitesi Veteriner Fakültesi Dergisi %P -1308-2817 %V 67 %N 4 %R doi: 10.33988/auvfd.682682 %U 10.33988/auvfd.682682
ISNAD Grıgorova, Natalia , Gócza, Elen , Vachkova, Ekaterina . "Pilot study on cardiogenic differentiation capability of rabbit mesenchymal stem cells". Ankara Üniversitesi Veteriner Fakültesi Dergisi 67 / 4 (September 2020): 407-412 . https://doi.org/10.33988/auvfd.682682
AMA Grıgorova N , Gócza E , Vachkova E . Pilot study on cardiogenic differentiation capability of rabbit mesenchymal stem cells. Ankara Univ Vet Fak Derg. 2020; 67(4): 407-412.
Vancouver Grıgorova N , Gócza E , Vachkova E . Pilot study on cardiogenic differentiation capability of rabbit mesenchymal stem cells. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2020; 67(4): 407-412.
IEEE N. Grıgorova , E. Gócza and E. Vachkova , "Pilot study on cardiogenic differentiation capability of rabbit mesenchymal stem cells", Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 67, no. 4, pp. 407-412, Sep. 2020, doi:10.33988/auvfd.682682