Research Article
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Year 2020, Volume: 67 Issue: 4, 419 - 425, 01.09.2020

Bojan Stojanović Nenad đorđević Aleksandar Simić Aleksa Božičković Vesna Davidović Aleksandra Ivetić

https://doi.org/10.33988/auvfd.702257
Cited By: 1

Abstract

References

  • 1. Amer S, Hassanat F, Berthiaume R, et al (2012): Effects of water soluble carbohydrate content on ensiling characteristics, chemical composition and in vitro gas production of forage millet and forage sorghum silages. Anim Feed Sci Tech, 177, 23-29.
  • 2. AOAC (2002): Official methods of analysis. 17th ed. 1st revision. Association of Analytical Communities, Gaithersburg, MD, USA.
  • 3. Canevari WM, Putnam DH, Lanini WT, et al (2000): Overseeding and companion cropping in alfalfa. University of California, Agriculture and Natural Resources, Oakland, CA, USA.
  • 4. Coblentz WK, Abdelgadir IEO, Cochran RC, et al (1999): Degradability of forage proteins by in situ and in vitro enzymatic methods. J Dairy Sci, 82, 343-354.
  • 5. Dijkstra J, Reynolds, CK, Kebreab E, et al (2013): Challenges in ruminant nutrition: towards minimal nitrogen losses in cattle. 47-58. In: JW Oltjen, E Kebreab, H Lapierre (Ed), Energy and protein metabolism and nutrition in sustainable animal production. Wageningen Academic Publishers, EAAP publication No. 134, The Netherlands.
  • 6. Edmunds B, Südekum KH, Spiekers H, et al (2012): Estimating ruminal crude protein degradation of forages using in situ and in vitro techniques. Anim Feed Sci Tech, 175, 95-105.
  • 7. Elizalde JC, Merchen NR, Faulkner DB (1999): Fractionation of fiber and crude protein in fresh forages during the spring growth. J Anim Sci, 77, 476-484.
  • 8. Fijałkowska M, Pysera B, Lipiński K, et al (2015): Changes of nitrogen compounds during ensiling of high protein herbages: a review. Ann Anim Sci, 15, 289-305.
  • 9. Frank B, Persson M, Gustafsson G (2002): Feeding dairy cows for decreased ammonia emission. Livest Prod Sci, 76, 171-179.
  • 10. Gierus M, Herrmann A, Kruse S, et al (2006): Variation in the non-protein nitrogen content (fraction A) of several forages during the growing period. Grassland Sci Eur, 11, 595-597.
  • 11. Grabber JH (2009a): Protein fractions in forage legumes containing protein-binding polyphenols: Freeze-drying vs. conservation as hay or silage. Anim Feed Sci Tech., 151, 324-329.
  • 12. Grabber JH (2009b): Forage management effects on protein and fiber fractions, protein degradability, and dry matter yield of red clover conserved as silage. Anim Feed Sci Tech, 154, 284-291.
  • 13. Guo XS, Ding WR, Han JG, et al (2008): Characterization of protein fractions and amino acids in ensiled alfalfa treated with different chemical additives. Anim Feed Sci Tech, 142, 89-98.
  • 14. Higgs RJ, Chase LE, Ross DA, et al (2015): Updating the Cornell Net Carbohydrate and Protein System feed library and analyzing model sensitivity to feed inputs. J Dairy Sci, 98, 6340-6360.
  • 15. Hristov AN, Bannink A, Crompton LA, et al (2019): Invited review: Nitrogen in ruminant nutrition: A review of measurement techniques. J Dairy Sci, 102, 5811-5852.
  • 16. Hyams Development (2017): CurveExpert Professional, Version 2.6.3. Available at www.curveexpert.net. (Accessed March 01, 2020).
  • 17. Krawutschke M, Weiher N, Thaysen J, et al (2013): The effect of cultivar on the changes in protein quality during wilting and ensiling of red clover (Trifolium pratense L.). J Agr Sci Cambridge, 151, 506-518.
  • 18. Licitra G, Hernandez T, Van Soest PJ (1996): Standardization of procedures for nitrogen fractionation of ruminant feeds. Anim Feed Sci Tech, 57, 347-358. 19. Makoni NF, Shelford JA, Nakai S, et al (1993): Characterization of protein fractions in fresh, wilted, and ensiled alfalfa. J Dairy Sci, 76, 1934-1944.
  • 20. Mathis CP, Cochran RC, Vanzant ES, et al (2001): A collaborative study comparing an in situ protocol with single time-point enzyme assays for estimating ruminal protein degradability of different forages. Anim Feed Sci Tech, 93, 31-42.
  • 21. Owens VN, Albrecht KA, Muck RE (1999): Protein degradation and ensiling characteristics of red clover and alfalfa wilted under varying levels of shade. Can J Plant Sci, 79, 209-222.
  • 22. Pedersen JF, Fritz JO (2000): Forages and Fodder. 797-810. In: CW Smith, RA Frederiksen (Ed), Sorghum: origin, history, technology and production. John Wiley & Sons Inc, Hoboken, NJ, USA.
  • 23. Reynal SM, Broderick GA (2005): Effect of dietary level of rumen-degraded protein on production and nitrogen metabolism in lactating dairy cows. J Dairy Sci, 88, 4045-4064.
  • 24. Sniffen CJ, O’Connor JD, Van Soest PJ, et al (1992): A net carbohydrate and protein system for evaluating cattle diets: II. Carbohydrate and protein availability. J Anim Sci, 70, 3562-3577.
  • 25. StatSoft. Inc. (2003): Statistica (data analysis software system), version 6.0. Available at www.statsoft.com. (Accessed March 01, 2020).
  • 26. Tremblay GF, Michaud R, Bélanger G (2003): Protein fractions and ruminal undegradable proteins in alfalfa. Can J Plant Sci, 83, 555-559.

The in vitro protein degradability of legume and sudan grass forage types and ensiled mixtures

Year 2020, Volume: 67 Issue: 4, 419 - 425, 01.09.2020

Bojan Stojanović Nenad đorđević Aleksandar Simić Aleksa Božičković Vesna Davidović Aleksandra Ivetić

https://doi.org/10.33988/auvfd.702257
Cited By: 1

Abstract

This study examined the in vitro crude protein (CP) degradability of wilted, dried for hay and ensiled Medicago sativa, Trifolium pratense and Sorghum sudanense, as well as of ensiled legume-sudan grass mixtures. The rumen degradable protein (RDP) was estimated using the latest Cornell net carbohydrate and protein system (CNCPS v6.5) and with the Streptomyces griseus protease assay (48 h of incubation). Sudan grass forages were the lowest in moderately degradable CP and the highest in CP fraction C, while red clover showed the least values for the soluble true protein like alfalfa forages for slowly degradable protein bound in neutral detergent fibre (NDF). Ensiling of legume-sudan grass mixtures decreases N-NH3 content by 12.2 and 5.1% in comparison with alfalfa and red clover silages, respectively. The RDP values for sudan grass (fresh, wilted, hay) were lower by 7.2 to 7.9% or 10.4 to 15.7% (CNCPS or S. griseus procedure) and up to 0.8 or 5.3 to 9.7% in comparison with alfalfa and red clover, respectively. The reduction of protein degradability in ensiled legume-sudan grass mixtures was 5.5 or 6.1% and 1.5 or 3% compared to alfalfa and red clover silages, respectively. Due to higher rumen undegradable protein content, the sudan grass and legume-sudan grass mixtures may be efficiently used to improve the protein utilization in ruminant nutrition.

Keywords

alfalfa Protein fractions red clover ruminants sudan grass

References

  • 1. Amer S, Hassanat F, Berthiaume R, et al (2012): Effects of water soluble carbohydrate content on ensiling characteristics, chemical composition and in vitro gas production of forage millet and forage sorghum silages. Anim Feed Sci Tech, 177, 23-29.
  • 2. AOAC (2002): Official methods of analysis. 17th ed. 1st revision. Association of Analytical Communities, Gaithersburg, MD, USA.
  • 3. Canevari WM, Putnam DH, Lanini WT, et al (2000): Overseeding and companion cropping in alfalfa. University of California, Agriculture and Natural Resources, Oakland, CA, USA.
  • 4. Coblentz WK, Abdelgadir IEO, Cochran RC, et al (1999): Degradability of forage proteins by in situ and in vitro enzymatic methods. J Dairy Sci, 82, 343-354.
  • 5. Dijkstra J, Reynolds, CK, Kebreab E, et al (2013): Challenges in ruminant nutrition: towards minimal nitrogen losses in cattle. 47-58. In: JW Oltjen, E Kebreab, H Lapierre (Ed), Energy and protein metabolism and nutrition in sustainable animal production. Wageningen Academic Publishers, EAAP publication No. 134, The Netherlands.
  • 6. Edmunds B, Südekum KH, Spiekers H, et al (2012): Estimating ruminal crude protein degradation of forages using in situ and in vitro techniques. Anim Feed Sci Tech, 175, 95-105.
  • 7. Elizalde JC, Merchen NR, Faulkner DB (1999): Fractionation of fiber and crude protein in fresh forages during the spring growth. J Anim Sci, 77, 476-484.
  • 8. Fijałkowska M, Pysera B, Lipiński K, et al (2015): Changes of nitrogen compounds during ensiling of high protein herbages: a review. Ann Anim Sci, 15, 289-305.
  • 9. Frank B, Persson M, Gustafsson G (2002): Feeding dairy cows for decreased ammonia emission. Livest Prod Sci, 76, 171-179.
  • 10. Gierus M, Herrmann A, Kruse S, et al (2006): Variation in the non-protein nitrogen content (fraction A) of several forages during the growing period. Grassland Sci Eur, 11, 595-597.
  • 11. Grabber JH (2009a): Protein fractions in forage legumes containing protein-binding polyphenols: Freeze-drying vs. conservation as hay or silage. Anim Feed Sci Tech., 151, 324-329.
  • 12. Grabber JH (2009b): Forage management effects on protein and fiber fractions, protein degradability, and dry matter yield of red clover conserved as silage. Anim Feed Sci Tech, 154, 284-291.
  • 13. Guo XS, Ding WR, Han JG, et al (2008): Characterization of protein fractions and amino acids in ensiled alfalfa treated with different chemical additives. Anim Feed Sci Tech, 142, 89-98.
  • 14. Higgs RJ, Chase LE, Ross DA, et al (2015): Updating the Cornell Net Carbohydrate and Protein System feed library and analyzing model sensitivity to feed inputs. J Dairy Sci, 98, 6340-6360.
  • 15. Hristov AN, Bannink A, Crompton LA, et al (2019): Invited review: Nitrogen in ruminant nutrition: A review of measurement techniques. J Dairy Sci, 102, 5811-5852.
  • 16. Hyams Development (2017): CurveExpert Professional, Version 2.6.3. Available at www.curveexpert.net. (Accessed March 01, 2020).
  • 17. Krawutschke M, Weiher N, Thaysen J, et al (2013): The effect of cultivar on the changes in protein quality during wilting and ensiling of red clover (Trifolium pratense L.). J Agr Sci Cambridge, 151, 506-518.
  • 18. Licitra G, Hernandez T, Van Soest PJ (1996): Standardization of procedures for nitrogen fractionation of ruminant feeds. Anim Feed Sci Tech, 57, 347-358. 19. Makoni NF, Shelford JA, Nakai S, et al (1993): Characterization of protein fractions in fresh, wilted, and ensiled alfalfa. J Dairy Sci, 76, 1934-1944.
  • 20. Mathis CP, Cochran RC, Vanzant ES, et al (2001): A collaborative study comparing an in situ protocol with single time-point enzyme assays for estimating ruminal protein degradability of different forages. Anim Feed Sci Tech, 93, 31-42.
  • 21. Owens VN, Albrecht KA, Muck RE (1999): Protein degradation and ensiling characteristics of red clover and alfalfa wilted under varying levels of shade. Can J Plant Sci, 79, 209-222.
  • 22. Pedersen JF, Fritz JO (2000): Forages and Fodder. 797-810. In: CW Smith, RA Frederiksen (Ed), Sorghum: origin, history, technology and production. John Wiley & Sons Inc, Hoboken, NJ, USA.
  • 23. Reynal SM, Broderick GA (2005): Effect of dietary level of rumen-degraded protein on production and nitrogen metabolism in lactating dairy cows. J Dairy Sci, 88, 4045-4064.
  • 24. Sniffen CJ, O’Connor JD, Van Soest PJ, et al (1992): A net carbohydrate and protein system for evaluating cattle diets: II. Carbohydrate and protein availability. J Anim Sci, 70, 3562-3577.
  • 25. StatSoft. Inc. (2003): Statistica (data analysis software system), version 6.0. Available at www.statsoft.com. (Accessed March 01, 2020).
  • 26. Tremblay GF, Michaud R, Bélanger G (2003): Protein fractions and ruminal undegradable proteins in alfalfa. Can J Plant Sci, 83, 555-559.
There are 25 citations in total.

Details

Primary Language English
Subjects Veterinary Surgery
Journal Section Research Article
Authors

Bojan Stojanović 0000-0001-5057-1790

Nenad đorđević 0000-0003-4196-4773

Aleksandar Simić 0000-0002-7605-3796

Aleksa Božičković 0000-0002-8671-4906

Vesna Davidović 0000-0003-0764-3183

Aleksandra Ivetić 0000-0003-2762-1870

Publication Date September 1, 2020
Published in Issue Year 2020Volume: 67 Issue: 4

Cite

APA Stojanović, B., đorđević, N., Simić, A., Božičković, A., et al. (2020). The in vitro protein degradability of legume and sudan grass forage types and ensiled mixtures. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 67(4), 419-425. https://doi.org/10.33988/auvfd.702257
AMA Stojanović B, đorđević N, Simić A, Božičković A, Davidović V, Ivetić A. The in vitro protein degradability of legume and sudan grass forage types and ensiled mixtures. Ankara Univ Vet Fak Derg. September 2020;67(4):419-425. doi:10.33988/auvfd.702257
Chicago Stojanović, Bojan, Nenad đorđević, Aleksandar Simić, Aleksa Božičković, Vesna Davidović, and Aleksandra Ivetić. “The in Vitro Protein Degradability of Legume and Sudan Grass Forage Types and Ensiled Mixtures”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 67, no. 4 (September 2020): 419-25. https://doi.org/10.33988/auvfd.702257.
EndNote Stojanović B, đorđević N, Simić A, Božičković A, Davidović V, Ivetić A (September 1, 2020) The in vitro protein degradability of legume and sudan grass forage types and ensiled mixtures. Ankara Üniversitesi Veteriner Fakültesi Dergisi 67 4 419–425.
IEEE B. Stojanović, N. đorđević, A. Simić, A. Božičković, V. Davidović, and A. Ivetić, “The in vitro protein degradability of legume and sudan grass forage types and ensiled mixtures”, Ankara Univ Vet Fak Derg, vol. 67, no. 4, pp. 419–425, 2020, doi: 10.33988/auvfd.702257.
ISNAD Stojanović, Bojan et al. “The in Vitro Protein Degradability of Legume and Sudan Grass Forage Types and Ensiled Mixtures”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 67/4 (September 2020), 419-425. https://doi.org/10.33988/auvfd.702257.
JAMA Stojanović B, đorđević N, Simić A, Božičković A, Davidović V, Ivetić A. The in vitro protein degradability of legume and sudan grass forage types and ensiled mixtures. Ankara Univ Vet Fak Derg. 2020;67:419–425.
MLA Stojanović, Bojan et al. “The in Vitro Protein Degradability of Legume and Sudan Grass Forage Types and Ensiled Mixtures”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 67, no. 4, 2020, pp. 419-25, doi:10.33988/auvfd.702257.
Vancouver Stojanović B, đorđević N, Simić A, Božičković A, Davidović V, Ivetić A. The in vitro protein degradability of legume and sudan grass forage types and ensiled mixtures. Ankara Univ Vet Fak Derg. 2020;67(4):419-25.

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