Research Article
Year 2021,
Volume: 68 Issue: 1, 33 - 38, 25.12.2020
Abstract
References
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- 2. Aitken RJ, Harkiss D, Buckingham D (1993): Relationship between iron-catalysed lipid peroxidation potential and human sperm function. J Reprod Fertil, 98, 257-265.
- 3. Anchordoquy JM, Anchordoquy JP, Testa JA, et al (2015): Influence of vascular endothelial growth factor and Cysteamine on in vitro bovine oocyte maturation and subsequent embryo development. Cell Biol Int, 39, 1090-1098.
- 4. Balasubramanian S, Rho GJ (2007): Effect of cysteamine supplementation of in vitro matured bovine oocytes on chilling sensitivity and development of embryos. Anim Reprod Sci, 98, 282-292.
- 5. Do VH, Walton S, Taylor-Robinson AW (2016): Improvements to in vitro culture media for use in bovine IVF. J Vet Sci Anim Husb, 4 , 205.
- 6. Duque P, Gomez E, Diaz E, et al (2003): Use of two serum during bovine embryo culture in vitro. Theriogenology, 59, 889-899.
- 7. Enginler SO, Ozdaş OB, Sandal AI, et al (2016): The effect of cysteamine and oviductal cells in different culture media on the development of sheep embryos. Kafkas Univ Vet Fak, 22, 139-145.
- 8. Gomez E, Rodriguez A, Munoz M, et al (2008): Serum free embryo culture medium improves in vitro survival of bovine blastocysts to vitrification. Theriogenology, 69, 1013-1021.
- 9. Guerin P, El Mouatassim S, Menezo Y (2001): Oxidative stress and protection against reactive oxygen species in the pre-implantation embryo and its surroundings. Hum Reprod Update, 7, 175-189.
- 10. Hajarian H, Aghaz F, Karami Shabankareh H (2017): Replacement of serum with sericin in in vitro maturation and culture media: Effects on embryonic developmental competence of Sanjabi sheep embryo during breeding season. Theriogenology, 92, 144-148.
- 11. Hammon DS, Wang S, Holyoak GR (2000): Effects of ammonia during different stages of culture on development of in vitro produced bovine embryos. Anim Reprod Sci, 59, 23-30.
- 12. Hong J, Lee E (2007): Intrafollicular amino acid concentration and the effect of amino acids in a defined maturation medium on porcine oocyte maturation, fertilization, and preimplantation development. Theriogenology, 68, 728-735.
- 13. Kharche SD, Agrawal S, Pathak J, et al (2016): Influence of cysteamine supplementation during in vitro culture of early stage caprine embryos on blastocyst production. Indian J Anim Sci, 86, 304-306.
- 14. Kitagawa Y, Suzuki K, Yoneda A, et al (2004): Effects of oxygen concentration and antioxidants on the in vitro developmental ability, production of reactive oxygen species (ROS), and DNA fragmentation in porcine embryos. Theriogenology, 62, 1186-1197.
- 15. Kobayashi M, Lee ES, Fukui Y (2006): Cysteamine or β-mercaptoethanol added to defined maturation medium improves blastocyst formation of porcine oocytes after intracytoplasmic sperm injection. Theriogenology, 65, 1191-1199.
- 16. Kuran M, Robinson JJ, Staines ME, et al (2001): Development and de novo protein synthetic activity of bovine embryos produced in vitro in different culture systems. Theriogenology, 55, 593-606.
- 17. Lane M, Gardner DK (1994): Increase in postimplantation development of cultured mouse embryos by amino acids and induction of fetal retardation and exencephaly by ammonium ions. J Reprod Fertil, 102, 305-312.
- 18. Moore K, Rodriguez-Sallaberry CJ, Kramer JM, et al (2007): In vitro production of bovine embryos in medium supplemented with a serum replacer: Effects on blastocyst development, cryotolerance and survival to term. Theriogenology, 68, 1316-1325.
- 19. Murillo-Ríos A, Maillo V, Muñoz M, et al (2017): Short- and long-term outcomes of the absence of protein during bovine blastocyst formation in vitro. Reprod Fert Develop, 29, 1064-1073.
- 20. Murillo A, Muñoz M, Martín-González D, et al (2017): Low serum concentration in bovine embryo culture enhances early blastocyst rates on Day-6 with quality traits in the expanded blastocyst stage similar to BSA-cultured embryos. Reprod Biology, 17, 162–171.
- 21. Oyamada T, Fukui Y (2004): Oxygen tension and medium supplements for in vitro maturation of bovine oocytes cultured individually in a chemically defined medium. J Reprod Develop, 50, 107-117.
- 22. Raty M, Ketoja E, Pitkanen T, et al (2011): In vitro maturation supplements affect developmental competence of bovine cumulus-oocyte complexes and embryo quality after vitrification. Cryobiology, 63, 245-255.
- 23. Reis A, Rooke JA, McCallum GJ, et al (2003): Consequences of exposure to serum, with or without vitamin E supplementation, in terms of the fatty acid content and viability of bovine blastocysts produced in vitro. Reprod Fert Develop, 15, 275-284.
- 24. Rooke JA, Ewen M, Mackie K, et al (2004): Effect of ammonium chloride on the growth and metabolism of bovine ovarian granulosa cells and the development of ovine oocytes matured in the presence of bovine granulosa cells previously exposed to ammonium chloride. Anim Reprod Sci, 84, 53-71.
- 25. Sağırkaya H, Mısırlıoğlu M, Kayaç A, et al (2007): Developmental potential of bovine oocytes cultured in different maturation and culture conditions. Anim Reprod Sci, 101, 225-240.
- 26. Sandal AI, Ozdaş OB (2015): Vitrification of in vitro-produced bovine embryos matured in modified TCM-199 medium. Turk J Vet Anim Sci, 39, 688-692.
- 27. Saragusty J, Arav A (2011): Current progress in oocyte and embryo cryopreservation by slow freezing and vitrification. Reproduction, 141, 1-19.
- 28. Sovernigo TC, Adona PR, Monzani PS, et al (2017): Effects of supplementation of medium with different antioxidants during in vitro maturation of bovine oocytes on subsequent embryo production. Reprod Domest Anim, 52, 561-569.
- 29. Thompson JG, McNaughton C, Gasparrini B, et al (2000): Effects of inhibitors and uncouplers of oxidative phosphorylation during compaction and blastulation of bovine embryos cultured in vitro. J Reprod Fertil, 118, 47-55.
- 30. Wan P, Hao Z, Zhou P, et al (2009): Effects of SOF and CR1 media on developmental competence and cell apoptosis of ovine in vitro fertilization embryos. Anim Reprod Sci, 114, 279-288.
- 31. Valk JVD, Brunner D, Smet KD, et al (2010): Optimization of chemically defined cell culture media - Replacing fetal bovine serum in mammalian in vitro methods. Toxicol in Vitro, 24, 1053-1063.
- 32. Van Soom A, Yuan YQ, Peelman LJ, et al (2002): Prevalence of apoptosis and inner cell allocation in bovine embryos cultured under different oxygen tensions with or without cysteine addition. Theriogenology, 57, 1453-1465.
- 33. Zabihi A, Shabankareh HK, Hajarian H, et al (2019): Resveratrol addition to in vitro maturation and in vitroculture media enhances developmental competence of sheep embryos. Domest Anim Endocrin, 68, 25-31.
- 34. Zolini AM, Carrascal-Trianaa E, Ruiz de Kinga A, et al (2019): Effect of addition of L-carnitine to media for oocyte maturation and embryo culture on development and cryotolerance of bovine embryos produced in vitro. Theriogenology, 133, 135-143.
Improvement of bovine in vitro embryo production by fetal calf serum and cysteamine supplementation and investigation of freezability
Abstract
The aim of this study was to investigate the effects of Cysteamine (Cys) and Fetal Calf Serum (FCS) in Synthetic Oviduct Fluid (SOF) and Charles and Rosenkrans (CR1aa) on the bovine in vitro embryo production and its ability of freeze. The oocytes were divided into two groups and allowed to mature in TCM-199, with and without cysteamine. They were divided into 4 subgroups according to whether they contain Cys and FCS in 2 different culture media (SOF and CR1aa). Accordingly, 8 groups were formed as SOF+FCS+Cys, SOF+Cys, SOF+FCS, S Dulbeco's OF, CR1aa+FCS+Cys, CR1aa+Cys, CR1aa+FCS and CR1aa. Embryos were cultured for 7-9 days and were frozen using vitrification method. Development of embryos was observed during the first 24 hr post-thaw period. The highest rate of compact morula was 20% in SOF+FCS+Cys and 19.4% in CR1aa+FCS+Cys. The rate of blastocyst in SOF+FCS and CR1aa+FCS were found 17.9% and 15.4% respectively and the difference between groups was statistically significant (P<0.05). During the 12 hr vitality assessment, the highest rate was determined in SOF+FCS (47%). The results indicate that FCS has a positive effect in reaching compact morula and blastocyst regardless of the presence of the culture medium or antioxidant used. There was no statistically difference between the values of post-thaw embryos.
Keywords
References
- 1. Abe H, Yamashita S, Itoh T, et al (1999): Ultrastructure of bovine embryos developed from in vitro matured and fertilized oocytes: Comparative morphological evaluation of embryos cultured either in serum free medium or serum supplemented medium. Mol Reprod Dev, 53, 325-335.
- 2. Aitken RJ, Harkiss D, Buckingham D (1993): Relationship between iron-catalysed lipid peroxidation potential and human sperm function. J Reprod Fertil, 98, 257-265.
- 3. Anchordoquy JM, Anchordoquy JP, Testa JA, et al (2015): Influence of vascular endothelial growth factor and Cysteamine on in vitro bovine oocyte maturation and subsequent embryo development. Cell Biol Int, 39, 1090-1098.
- 4. Balasubramanian S, Rho GJ (2007): Effect of cysteamine supplementation of in vitro matured bovine oocytes on chilling sensitivity and development of embryos. Anim Reprod Sci, 98, 282-292.
- 5. Do VH, Walton S, Taylor-Robinson AW (2016): Improvements to in vitro culture media for use in bovine IVF. J Vet Sci Anim Husb, 4 , 205.
- 6. Duque P, Gomez E, Diaz E, et al (2003): Use of two serum during bovine embryo culture in vitro. Theriogenology, 59, 889-899.
- 7. Enginler SO, Ozdaş OB, Sandal AI, et al (2016): The effect of cysteamine and oviductal cells in different culture media on the development of sheep embryos. Kafkas Univ Vet Fak, 22, 139-145.
- 8. Gomez E, Rodriguez A, Munoz M, et al (2008): Serum free embryo culture medium improves in vitro survival of bovine blastocysts to vitrification. Theriogenology, 69, 1013-1021.
- 9. Guerin P, El Mouatassim S, Menezo Y (2001): Oxidative stress and protection against reactive oxygen species in the pre-implantation embryo and its surroundings. Hum Reprod Update, 7, 175-189.
- 10. Hajarian H, Aghaz F, Karami Shabankareh H (2017): Replacement of serum with sericin in in vitro maturation and culture media: Effects on embryonic developmental competence of Sanjabi sheep embryo during breeding season. Theriogenology, 92, 144-148.
- 11. Hammon DS, Wang S, Holyoak GR (2000): Effects of ammonia during different stages of culture on development of in vitro produced bovine embryos. Anim Reprod Sci, 59, 23-30.
- 12. Hong J, Lee E (2007): Intrafollicular amino acid concentration and the effect of amino acids in a defined maturation medium on porcine oocyte maturation, fertilization, and preimplantation development. Theriogenology, 68, 728-735.
- 13. Kharche SD, Agrawal S, Pathak J, et al (2016): Influence of cysteamine supplementation during in vitro culture of early stage caprine embryos on blastocyst production. Indian J Anim Sci, 86, 304-306.
- 14. Kitagawa Y, Suzuki K, Yoneda A, et al (2004): Effects of oxygen concentration and antioxidants on the in vitro developmental ability, production of reactive oxygen species (ROS), and DNA fragmentation in porcine embryos. Theriogenology, 62, 1186-1197.
- 15. Kobayashi M, Lee ES, Fukui Y (2006): Cysteamine or β-mercaptoethanol added to defined maturation medium improves blastocyst formation of porcine oocytes after intracytoplasmic sperm injection. Theriogenology, 65, 1191-1199.
- 16. Kuran M, Robinson JJ, Staines ME, et al (2001): Development and de novo protein synthetic activity of bovine embryos produced in vitro in different culture systems. Theriogenology, 55, 593-606.
- 17. Lane M, Gardner DK (1994): Increase in postimplantation development of cultured mouse embryos by amino acids and induction of fetal retardation and exencephaly by ammonium ions. J Reprod Fertil, 102, 305-312.
- 18. Moore K, Rodriguez-Sallaberry CJ, Kramer JM, et al (2007): In vitro production of bovine embryos in medium supplemented with a serum replacer: Effects on blastocyst development, cryotolerance and survival to term. Theriogenology, 68, 1316-1325.
- 19. Murillo-Ríos A, Maillo V, Muñoz M, et al (2017): Short- and long-term outcomes of the absence of protein during bovine blastocyst formation in vitro. Reprod Fert Develop, 29, 1064-1073.
- 20. Murillo A, Muñoz M, Martín-González D, et al (2017): Low serum concentration in bovine embryo culture enhances early blastocyst rates on Day-6 with quality traits in the expanded blastocyst stage similar to BSA-cultured embryos. Reprod Biology, 17, 162–171.
- 21. Oyamada T, Fukui Y (2004): Oxygen tension and medium supplements for in vitro maturation of bovine oocytes cultured individually in a chemically defined medium. J Reprod Develop, 50, 107-117.
- 22. Raty M, Ketoja E, Pitkanen T, et al (2011): In vitro maturation supplements affect developmental competence of bovine cumulus-oocyte complexes and embryo quality after vitrification. Cryobiology, 63, 245-255.
- 23. Reis A, Rooke JA, McCallum GJ, et al (2003): Consequences of exposure to serum, with or without vitamin E supplementation, in terms of the fatty acid content and viability of bovine blastocysts produced in vitro. Reprod Fert Develop, 15, 275-284.
- 24. Rooke JA, Ewen M, Mackie K, et al (2004): Effect of ammonium chloride on the growth and metabolism of bovine ovarian granulosa cells and the development of ovine oocytes matured in the presence of bovine granulosa cells previously exposed to ammonium chloride. Anim Reprod Sci, 84, 53-71.
- 25. Sağırkaya H, Mısırlıoğlu M, Kayaç A, et al (2007): Developmental potential of bovine oocytes cultured in different maturation and culture conditions. Anim Reprod Sci, 101, 225-240.
- 26. Sandal AI, Ozdaş OB (2015): Vitrification of in vitro-produced bovine embryos matured in modified TCM-199 medium. Turk J Vet Anim Sci, 39, 688-692.
- 27. Saragusty J, Arav A (2011): Current progress in oocyte and embryo cryopreservation by slow freezing and vitrification. Reproduction, 141, 1-19.
- 28. Sovernigo TC, Adona PR, Monzani PS, et al (2017): Effects of supplementation of medium with different antioxidants during in vitro maturation of bovine oocytes on subsequent embryo production. Reprod Domest Anim, 52, 561-569.
- 29. Thompson JG, McNaughton C, Gasparrini B, et al (2000): Effects of inhibitors and uncouplers of oxidative phosphorylation during compaction and blastulation of bovine embryos cultured in vitro. J Reprod Fertil, 118, 47-55.
- 30. Wan P, Hao Z, Zhou P, et al (2009): Effects of SOF and CR1 media on developmental competence and cell apoptosis of ovine in vitro fertilization embryos. Anim Reprod Sci, 114, 279-288.
- 31. Valk JVD, Brunner D, Smet KD, et al (2010): Optimization of chemically defined cell culture media - Replacing fetal bovine serum in mammalian in vitro methods. Toxicol in Vitro, 24, 1053-1063.
- 32. Van Soom A, Yuan YQ, Peelman LJ, et al (2002): Prevalence of apoptosis and inner cell allocation in bovine embryos cultured under different oxygen tensions with or without cysteine addition. Theriogenology, 57, 1453-1465.
- 33. Zabihi A, Shabankareh HK, Hajarian H, et al (2019): Resveratrol addition to in vitro maturation and in vitroculture media enhances developmental competence of sheep embryos. Domest Anim Endocrin, 68, 25-31.
- 34. Zolini AM, Carrascal-Trianaa E, Ruiz de Kinga A, et al (2019): Effect of addition of L-carnitine to media for oocyte maturation and embryo culture on development and cryotolerance of bovine embryos produced in vitro. Theriogenology, 133, 135-143.
There are 34 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery |
| Journal Section | Research Article |
| Authors | |
| Publication Date | December 25, 2020 |
| Published in Issue | Year 2021Volume: 68 Issue: 1 |
