Haemophilus somnus was isolated from two cows with
metritis slaughtered in a local abatloir. Uterine samples collected with steril
swabs were seeded onto several culture media. The organisms grew well only
on brain-heart infusion agar supplemented with blood and yeast extarct (BH/BrE) in pure culture. The colonies were translucent, greyish, round, and reached
the diameter of 0.5 mm af ter 24 hours of incubation. The isolated bacteria were
identified as Haemophilus somnus on the basis of morphological, cultural and
biochemical characteristics. The organism requiered serum and carbon dioxide for growt/z. The isolate was Gram negative, small, pleomorphic coccobaciUi
and fermented glucose, produce oxidase bl/t did not catalase. For the canlirmation
of isolates as H. somnus a slide agglutilZation test was alsa made. T/ze isolation
of agent in pure culture suggested t/zat this organism was the cause of pathologic
condition.
Primary Language | English |
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Subjects | Veterinary Surgery |
Journal Section | Research Article |
Authors | |
Publication Date | January 1, 1986 |
Published in Issue | Year 1986Volume: 33 Issue: 01 |