Research Article
Year 2021,
Volume: 68 Issue: 2, 97 - 105, 31.03.2021
Abstract
Supporting Institution
Van Yüzüncü Yıl University
Project Number
YYUBAP/TSA-2018-7456
References
- Araoz V, da Silva Silveira C, More G, et al (2019): Fatal Sarcocystis cruzi-induced eosinophilic myocarditis in a heifer in Uruguay. J Vet Diagn Invest, 31, 656-660.
- Arslan MÖ, Umur Ş (1997): The prevalence of sarcocystiosis in cattle in Kars and Erzurum Province, Türkiye. Türkiye Parazitol Derg, 21, 417-420.
- Bertin M, Lemieux D, Rossero A, et al (2014): Sarcocystis hominis is frequently associated with bovine eosinophilic myositis. Renc Rech Ruminants, 21, 321-324.
- Chiesa F, Muratore E, Dalmasso A, et al (2013): A new molecular approach to assess the occurrence of Sarcocystis spp. in cattle and products thereof: preliminary data. Ital J Food Saf, 2, e41.
- Domenis L, Peletto S, Sacchi L, et al (2011): Detection of a morphogenetically novel Sarcocystis hominis-like in the context of a prevalence study in semi-intensively bred cattle in Italy. Parasitol Res, 109, 1677-1687.
- Dubey JP (2015): Foodborne and waterborne zoonotic sarcocystosis. Food Waterborne Parasitol, 1, 2-11.
- El-Kady AM, Hussein NM, Hassan AA (2018): First molecular characterization of Sarcocystis spp. in cattle in Qena Governorate, Upper Egypt. J Parasit Dis, 42, 114-121.
- Fayer R, Esposito DH, Dubey JP (2015): Human infections with Sarcocystis species. Clin Microbiol Rev, 28, 295-311.
- Freiler PF, Mayhew IG, Pollock R (1979): Bovine saccocystosis: pathologic features of naturally occurring infection with Sarcocystis cruzi. Am J Vet Res, 40, 651-657.
- Gjerde B (2016): Molecular characterisation of Sarcocystis bovifelis, Sarcocystis bovini n. sp., Sarcocystis hirsuta and Sarcocystis cruzi from cattle (Bos taurus) and Sarcocystis sinensis from water buffaloes (Bubalus bubalis). Parasitol Res, 115, 1473-1492.
- Goncalo JA (2014): Muscle parasites by Sarcocystis spp. and Cysticercus bovis (Taenia saginata) in cattle of the Azores (In Portuguese). PhD Thesis. University of Lisbon, Lisbon, Portugal.
- Güçlü F, Aldemir OS, Güler L (2004): Differential identification of cattle Sarcocystis spp. by random amplified Polymorphic DNA-Polymerase chain reaction (RAPD-PCR). Revue Med Vet, 155, 440-444.
- Hall TA (1999): BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser, 41, 95-98.
- Hamidinejat H, Razi Jalali MH, Nabavi L (2010): Survey on Sarcocystis Infection in Slaughtered Cattle in South-West of Iran, Emphasized on Evaluation of Muscle Squash in Comparison with Digestion Method. J Animal Vet Adv, 9, 1724-1726.
- Hoeve-Bakker BJA, van der Giessen JWB, Franssen FFJ (2019): Molecular identification targeting cox1 and 18S genes confirms the high prevalence of Sarcocystis spp. in cattle in the Netherlands. Int J Parasitol, 49, 859-866.
- Hooshyar H, Abbaszadeh Z, Sharafati-Chaleshtori R, et al (2017): Molecular identification of Sarcocystis species in raw hamburgers using PCR-RFLP method in Kashan, central Iran. J Parasit Dis, 41, 1001-1005.
- Hornok S, Mester A, Takacs N, et al (2015): Sarcocystis-infection of cattle in Hungary. Parasit Vectors, 8, 69.
- Imre K, Dărăbuș G, Tîrziu E, et al (2019): Sarcocystis spp. in Romanian slaughtered cattle: Molecular characterization and epidemiological significance of the findings. Biomed Res Int, Article ID 4123154.
- Januskevicius V, Januskeviciene G, Prakas P, et al (2019): Prevalence and intensity of Sarcocystis spp. infection in animals slaughtered for food in Lithuania. Vet Med (Praha), 64, 149-157.
- Kamber U, Arslan MÖ, Gülbaz G, et al (2018): Identification of Sarcocystis spp. by polymerase chain reaction and microscopic examination in various beef products (minced meat, meatballs, fermented sausage). Turk J Vet Anim Sci, 42, 1-6.
- Kumar S, Stecher G, Tamura K (2016): MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol, 33, 1870-1874.
- Lassen B, Talvik H (2009): Parasitic protozoans in livestock and pets in Estonia. Review. Vet Zootech-Lith (Vet Med Zoot), 46, 30-36.
- Meistro S, Peletto, S, Pezzolato M, et al (2015): Sarcocystis spp. prevalence in bovine minced meat: a histological and molecular study. Ital J Food Saf, 4, 4626.
- Moghaddam Ahmadi M, Hajimohammadi B, Eslami G (2015): First identification of Sarcocystis hominis in Iranian traditional hamburger. J Parasit Dis, 39, 770-772.
- More G, Abrahamovich P, Jurado S, et al (2011): Prevalence of Sarcocystis spp. in Argentinean Cattle. Vet Parasitol, 177, 162-165.
- More G, Pantchev A, Skuballa J, et al (2014): Sarcocystis sinensis is the most prevalent thick-walled Sarcocystis species in beef on sale for consumers in Germany. Parasitol Res, 113, 2223-2230.
- Moussa MM, Hiekal FA, El-Hoshy SM, et al (2016): Characterization of Sarcocystis species based on traditional and molecular methods in imported frozen buffalo meat in Egypt. Alexandria J Vet Sci, 51, 155-161.
- Murata R, Suzuki J, Hyuga A, et al (2018): Molecular identification and characterization of Sarcocystis spp. in horsemeat and beef marketed in Japan. Parasite, 25, 27.
- Nourollahi-Fard SR, Asghari M, Nouri F (2009): Survey of Sarcocystis infection in slaughtered cattle in Kerman, Iran. Trop Anim Health Prod, 41, 1633-1636.
- Okur H, Kandemir O, Şahin I (1995): An investigation on Sarcocystis species in the cattle and the sheep in Bayburt. T Parazitol Derg, 19, 113-118.
- Özer E (1988): Elazığ mezbahasında kesilen sığır ve mandalarda Sarcocystis türleri ve insidensi üzerinde araştırmalar. TU Vet Hay Derg, 12, 130-139.
- Prakas P, Butkauskas D (2012): Protozoan parasites from genus Sarcocystis and their investigations in Lithuania. Ekologija, 58, 45-58.
- Pritt B, Trainer T, Simmons-Arnold L, et al (2008): Detection of Sarcocystis parasites in retail beef: A regional survey combining histological and genetic detection methods. J Food Prot, 71, 2144-2147.
- Ren M, Wu F, Zou Y, et al (2019): Molecular characterization of Sarcocystis species isolated from Chinese buffaloes in Guizhou province based on 18S rRNA and cox1 sequences. Mitochondrial DNA Part B, 4, 637-641.
- Rubiola S, Chiesa F, Zanet S, et al (2018): Molecular identification of Sarcocystis spp. in cattle: Partial sequencing of cytochrome c oxidase subunit 1 (COI). Ital J Food Saf, 7, 7725.
- Sancak YC, Akdemir C, Berktaş M (1995): Van piyasasında tüketime sunulan et ve et ürünlerinde sarcosporidiosis’in yayılışı. Van Sağ Bil Derg, 2, 114-116.
- Sevinç F (2013): Sığırlarda Sarcosporidiosis. 75-82. In: MA Özcel (Ed), Veteriner Hekimliğinde Parazit Hastalıkları. Türkiye Parazitoloji Derneği, İzmir.
- Silva NRS, Rodrigues RJD, Araujo FAP, et al (2002): Detection of bovine Sarcocystis cruzi cysts in cardiac muscles: A new technique of concentration for diagnostic. Acta Sci Vet, 30, 127-129.
- Taşcı S, Toparlak M, Gül Y (1989): Van mezbahasında kesilen sığırlarda sarcosporidiosis’in yayılışı. Ankara Univ Vet Fak, 36, 254-259.
- Taylor MA, Boes J, Boireau P, et al (2010): Developent of harmonised schemes for the monitoring and reporting of Sarcocystis in animals and foodstuffs in the European Union. Available at http://www.efsa.europa.eu/ en/supporting/pub/33e.htm. (Accessed May 12, 2020).
- Tüzer E, Demir S, Aydın O (1987): Bursa yöresi sığırlarında sarcosporidiozis. İstanbul Üniv Vet Fak Derg, 13, 21-30.
- Vangeel L, Houf K, Chiers K, et al (2007): Molecular based identification of sarcocystis hominis in Belgian minced beef. J Food Prot, 70, 1523-1526.
- Yang ZQ, Li QQ, Zuo YX, et al (2002): Characterization of Sarcocystis species in domestic animals using a PCR-RFLP analysis of variation in the 18S rRNA gene: A cost effective and simple technique for routine species identification. Exp Parasitol, 102, 212-217.
Molecular identification using 18S ribosomal RNA of Sarcocystis spp. in bovine minced meat in Van Province, Turkey
Abstract
This study aimed to determine the prevalence and molecular characterization of Sarcocystis spp. in bovine minced meat that is sold in various grocery stores and butcher shops in Van Province of Turkey. For this purpose a total of 150 samples were obtained from İpekyolu, Tuşba, Edremit, Erciş, and Gevaş districts of Van Province in monthly periods from May to October 2019. 28% (42/150) were found positive for Sarcocystis species as a result of the microscopic analyses and 96.6% (145/150) were found positive for Sarcocystis species as a result of the multiplex-PCR and RFLP methods. Sarcocystis cruzi (96.6%) was detected in all samples that were detected positive using molecular methods. Sarcocystis hominis-like was found in 143 (95%) samples whereas S. hirsuta was detected in only 4 (2.6%) samples. According to the Basic Local Alignment Search Tool (BLASTN) analysis of the 18S rRNA gene region of the S. cruzi (MN832695) and S. hirsuta (MN832697) isolates, they showed 100% similarity to the samples (MH681972; MH681973) that were submitted to GenBank from China. The BLASTN analysis of the 18S rRNA gene region of the S. hominis-like isolate (MN832696) revealed that it was 99.45% identical to the S. bovini (KT901155) isolated from a water buffalo in New Zealand. In conclusion, the molecular characterization of Sarcocystis spp. has been provided for the first time in Van Province, and the first unverified scientific data for S. bovini has been established in this study.
Keywords
Project Number
YYUBAP/TSA-2018-7456
References
- Araoz V, da Silva Silveira C, More G, et al (2019): Fatal Sarcocystis cruzi-induced eosinophilic myocarditis in a heifer in Uruguay. J Vet Diagn Invest, 31, 656-660.
- Arslan MÖ, Umur Ş (1997): The prevalence of sarcocystiosis in cattle in Kars and Erzurum Province, Türkiye. Türkiye Parazitol Derg, 21, 417-420.
- Bertin M, Lemieux D, Rossero A, et al (2014): Sarcocystis hominis is frequently associated with bovine eosinophilic myositis. Renc Rech Ruminants, 21, 321-324.
- Chiesa F, Muratore E, Dalmasso A, et al (2013): A new molecular approach to assess the occurrence of Sarcocystis spp. in cattle and products thereof: preliminary data. Ital J Food Saf, 2, e41.
- Domenis L, Peletto S, Sacchi L, et al (2011): Detection of a morphogenetically novel Sarcocystis hominis-like in the context of a prevalence study in semi-intensively bred cattle in Italy. Parasitol Res, 109, 1677-1687.
- Dubey JP (2015): Foodborne and waterborne zoonotic sarcocystosis. Food Waterborne Parasitol, 1, 2-11.
- El-Kady AM, Hussein NM, Hassan AA (2018): First molecular characterization of Sarcocystis spp. in cattle in Qena Governorate, Upper Egypt. J Parasit Dis, 42, 114-121.
- Fayer R, Esposito DH, Dubey JP (2015): Human infections with Sarcocystis species. Clin Microbiol Rev, 28, 295-311.
- Freiler PF, Mayhew IG, Pollock R (1979): Bovine saccocystosis: pathologic features of naturally occurring infection with Sarcocystis cruzi. Am J Vet Res, 40, 651-657.
- Gjerde B (2016): Molecular characterisation of Sarcocystis bovifelis, Sarcocystis bovini n. sp., Sarcocystis hirsuta and Sarcocystis cruzi from cattle (Bos taurus) and Sarcocystis sinensis from water buffaloes (Bubalus bubalis). Parasitol Res, 115, 1473-1492.
- Goncalo JA (2014): Muscle parasites by Sarcocystis spp. and Cysticercus bovis (Taenia saginata) in cattle of the Azores (In Portuguese). PhD Thesis. University of Lisbon, Lisbon, Portugal.
- Güçlü F, Aldemir OS, Güler L (2004): Differential identification of cattle Sarcocystis spp. by random amplified Polymorphic DNA-Polymerase chain reaction (RAPD-PCR). Revue Med Vet, 155, 440-444.
- Hall TA (1999): BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser, 41, 95-98.
- Hamidinejat H, Razi Jalali MH, Nabavi L (2010): Survey on Sarcocystis Infection in Slaughtered Cattle in South-West of Iran, Emphasized on Evaluation of Muscle Squash in Comparison with Digestion Method. J Animal Vet Adv, 9, 1724-1726.
- Hoeve-Bakker BJA, van der Giessen JWB, Franssen FFJ (2019): Molecular identification targeting cox1 and 18S genes confirms the high prevalence of Sarcocystis spp. in cattle in the Netherlands. Int J Parasitol, 49, 859-866.
- Hooshyar H, Abbaszadeh Z, Sharafati-Chaleshtori R, et al (2017): Molecular identification of Sarcocystis species in raw hamburgers using PCR-RFLP method in Kashan, central Iran. J Parasit Dis, 41, 1001-1005.
- Hornok S, Mester A, Takacs N, et al (2015): Sarcocystis-infection of cattle in Hungary. Parasit Vectors, 8, 69.
- Imre K, Dărăbuș G, Tîrziu E, et al (2019): Sarcocystis spp. in Romanian slaughtered cattle: Molecular characterization and epidemiological significance of the findings. Biomed Res Int, Article ID 4123154.
- Januskevicius V, Januskeviciene G, Prakas P, et al (2019): Prevalence and intensity of Sarcocystis spp. infection in animals slaughtered for food in Lithuania. Vet Med (Praha), 64, 149-157.
- Kamber U, Arslan MÖ, Gülbaz G, et al (2018): Identification of Sarcocystis spp. by polymerase chain reaction and microscopic examination in various beef products (minced meat, meatballs, fermented sausage). Turk J Vet Anim Sci, 42, 1-6.
- Kumar S, Stecher G, Tamura K (2016): MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol, 33, 1870-1874.
- Lassen B, Talvik H (2009): Parasitic protozoans in livestock and pets in Estonia. Review. Vet Zootech-Lith (Vet Med Zoot), 46, 30-36.
- Meistro S, Peletto, S, Pezzolato M, et al (2015): Sarcocystis spp. prevalence in bovine minced meat: a histological and molecular study. Ital J Food Saf, 4, 4626.
- Moghaddam Ahmadi M, Hajimohammadi B, Eslami G (2015): First identification of Sarcocystis hominis in Iranian traditional hamburger. J Parasit Dis, 39, 770-772.
- More G, Abrahamovich P, Jurado S, et al (2011): Prevalence of Sarcocystis spp. in Argentinean Cattle. Vet Parasitol, 177, 162-165.
- More G, Pantchev A, Skuballa J, et al (2014): Sarcocystis sinensis is the most prevalent thick-walled Sarcocystis species in beef on sale for consumers in Germany. Parasitol Res, 113, 2223-2230.
- Moussa MM, Hiekal FA, El-Hoshy SM, et al (2016): Characterization of Sarcocystis species based on traditional and molecular methods in imported frozen buffalo meat in Egypt. Alexandria J Vet Sci, 51, 155-161.
- Murata R, Suzuki J, Hyuga A, et al (2018): Molecular identification and characterization of Sarcocystis spp. in horsemeat and beef marketed in Japan. Parasite, 25, 27.
- Nourollahi-Fard SR, Asghari M, Nouri F (2009): Survey of Sarcocystis infection in slaughtered cattle in Kerman, Iran. Trop Anim Health Prod, 41, 1633-1636.
- Okur H, Kandemir O, Şahin I (1995): An investigation on Sarcocystis species in the cattle and the sheep in Bayburt. T Parazitol Derg, 19, 113-118.
- Özer E (1988): Elazığ mezbahasında kesilen sığır ve mandalarda Sarcocystis türleri ve insidensi üzerinde araştırmalar. TU Vet Hay Derg, 12, 130-139.
- Prakas P, Butkauskas D (2012): Protozoan parasites from genus Sarcocystis and their investigations in Lithuania. Ekologija, 58, 45-58.
- Pritt B, Trainer T, Simmons-Arnold L, et al (2008): Detection of Sarcocystis parasites in retail beef: A regional survey combining histological and genetic detection methods. J Food Prot, 71, 2144-2147.
- Ren M, Wu F, Zou Y, et al (2019): Molecular characterization of Sarcocystis species isolated from Chinese buffaloes in Guizhou province based on 18S rRNA and cox1 sequences. Mitochondrial DNA Part B, 4, 637-641.
- Rubiola S, Chiesa F, Zanet S, et al (2018): Molecular identification of Sarcocystis spp. in cattle: Partial sequencing of cytochrome c oxidase subunit 1 (COI). Ital J Food Saf, 7, 7725.
- Sancak YC, Akdemir C, Berktaş M (1995): Van piyasasında tüketime sunulan et ve et ürünlerinde sarcosporidiosis’in yayılışı. Van Sağ Bil Derg, 2, 114-116.
- Sevinç F (2013): Sığırlarda Sarcosporidiosis. 75-82. In: MA Özcel (Ed), Veteriner Hekimliğinde Parazit Hastalıkları. Türkiye Parazitoloji Derneği, İzmir.
- Silva NRS, Rodrigues RJD, Araujo FAP, et al (2002): Detection of bovine Sarcocystis cruzi cysts in cardiac muscles: A new technique of concentration for diagnostic. Acta Sci Vet, 30, 127-129.
- Taşcı S, Toparlak M, Gül Y (1989): Van mezbahasında kesilen sığırlarda sarcosporidiosis’in yayılışı. Ankara Univ Vet Fak, 36, 254-259.
- Taylor MA, Boes J, Boireau P, et al (2010): Developent of harmonised schemes for the monitoring and reporting of Sarcocystis in animals and foodstuffs in the European Union. Available at http://www.efsa.europa.eu/ en/supporting/pub/33e.htm. (Accessed May 12, 2020).
- Tüzer E, Demir S, Aydın O (1987): Bursa yöresi sığırlarında sarcosporidiozis. İstanbul Üniv Vet Fak Derg, 13, 21-30.
- Vangeel L, Houf K, Chiers K, et al (2007): Molecular based identification of sarcocystis hominis in Belgian minced beef. J Food Prot, 70, 1523-1526.
- Yang ZQ, Li QQ, Zuo YX, et al (2002): Characterization of Sarcocystis species in domestic animals using a PCR-RFLP analysis of variation in the 18S rRNA gene: A cost effective and simple technique for routine species identification. Exp Parasitol, 102, 212-217.
There are 43 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery |
| Journal Section | Research Article |
| Authors | |
| Project Number | YYUBAP/TSA-2018-7456 |
| Publication Date | March 31, 2021 |
| Published in Issue | Year 2021Volume: 68 Issue: 2 |
