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Year 2021, Volume: 68 Issue: 2, 167 - 172, 31.03.2021
https://doi.org/10.33988/auvfd.756970

Abstract

References

  • Ball C, Awad F, Hutton S, et al (2017): Infectious bronchitis vaccine virus detection and part-S1 genetic variation following single or dual inoculation in broiler chicks. Avian Pathol, 46, 309-318.
  • Bassam RH, Hussein HA, Amer MM, et al (2019): Molecular Characterization of VAR2 among IBV Infected poultry in Lebanon. J Virol Sci, 5, 114-123.
  • Callison SA, Jackwood MW, Hilt DA (2001): Molecular characterization of infectious bronchitis virus isolates foreign to the United States and comparison with United States isolates. Avian Dis, 45, 492-499.
  • Cavanagh D (2007): Coronavirus avian infectious bronchitis virus. Vet Res, 38, 281-297.
  • Cook JK, Jackwood M, Jones RC (2012): The long view: 40 years of infectious bronchitis research. Avian Pathol, 41, 239-250.
  • De Wit JJ, Cook, JKA, Van der Heijden HM (2011): Infectious bronchitis virus variants: a review of the history, current situation and control measures. Avian Pathol, 40, 223-235.
  • Feng KY, Chen T, Zhang X, et al (2018): Molecular characteristic and pathogenicity analysis of a virulent recombinant avain infectious bronchitis virus isolated in China. Poult Sci, 97, 3519-3531.
  • Haqshenas G, Assasi K, Akrami H (2005): Isolation and molecular characterization of infectious bronchitis virüs isolate Shiraz IBV, by RT PCR and restriction enzyme analysis. Iran J Vet Res, 6, 70-78.
  • Kahya S, Coven F, Temelli S, et al (2013): Presence of IS/1494/06 genotype-related infectious bronchitis virus in breeder and broiler flocks in Turkey. Ankara Univ Vet Fak Derg, 60, 27-31.
  • King DJ, Cavanagh D (1991): Infectious bronchitis. 471-484. In: BW Calnek, HJ Barnes, CW Beard, WM Reid, HW Yoder (Eds), Diseases of Poultry. Iowa State University Press, Ames.
  • Lai M, Cavanagh D (1997): The molecular biology of coronaviruses. Adv Virus Res, 48, 1-100.
  • Meir R, Rosenblut E, Perl S, et al (2004): Identification of a novel nephropathogenic infectious bronchitis virus in Israel. Avian Dis, 48, 635-641.
  • Valastro V, Holmes EC, Britton P, et al (2016): S1 gene-based phylogeny of infectious bronchitis virus: an attempt to harmonize virus classification. Infect Genet Evol J, 39, 349-364.
  • Yılmaz H, Altan E, Çizmecigil UY, et al (2016): Phylogeny and S1 gene variation of infectious bronchitis virus detected in broilers and layers in Turkey. Avian Dis, 60, 596-602.
  • Yılmaz H, Çizmecigil UY, Gurel AB, et al (2017): Variants of Infectious Bronchitis Virus (Ibv) in chickens in Turkey and production of recombinant Ibv-N protein by using baculovirus expression system. Bulg J Vet Med, 1, 240-247.

Detection of Israel variant 2 (IS/1494/06) genotype of Infectious Bronchitis Virus in a layer chicken flock

Year 2021, Volume: 68 Issue: 2, 167 - 172, 31.03.2021
https://doi.org/10.33988/auvfd.756970

Abstract

The aim of this study was to determine an infectious bronchitis (IB) infection, caused by an Israel variant 2 (IS/1494/06)-like IBV, in a layer chicken flock regularly vaccinated with vaccines containing IBV H120 and 4/91 strains. Mild respiratory symptoms, drop in egg production and soft-shelled eggs and eventually death were observed in a layer chicken flock. Clinical samples from four diseased chickens were examined for the detection and genotyping of IBV by virus isolation, a commercial real time reverse transcription polymerase chain reaction (rRT-PCR) and nucleotide sequencing. Both Israel variant 2 (IS-Var2) and 793/B serotypes were detected from samples by rRT-PCR, but sequencing results of a 345 bp part of S1 gene revealed that our IBV isolate, HFT-IBV, was IS/1494/06 (IS-Var2)-like with the 97.7% genetic similarity. These results suggested that immunity against vaccination with a combination of different genotypes (H120 and 4/91) could not be protective for IS-Var2 IBV field infection. In addition, identification of genotypes from the clinical samples, such as swabs and organ samples by commercial rRT-PCR assays failed to find correct IBV genotype responsible for the IB infection, whereas analysis of nucleotide sequencing of S1 gene of IBV as a gold standard test undoubtedly detected causative genotype of the infection. Also, the findings indicated that there is an urgent need for consider genotype- or protectotype-match vaccination strategies in the field to prevent vaccine- and IB-dependent economic losses of the poultry sector and logically protect chickens from IBV infection.

References

  • Ball C, Awad F, Hutton S, et al (2017): Infectious bronchitis vaccine virus detection and part-S1 genetic variation following single or dual inoculation in broiler chicks. Avian Pathol, 46, 309-318.
  • Bassam RH, Hussein HA, Amer MM, et al (2019): Molecular Characterization of VAR2 among IBV Infected poultry in Lebanon. J Virol Sci, 5, 114-123.
  • Callison SA, Jackwood MW, Hilt DA (2001): Molecular characterization of infectious bronchitis virus isolates foreign to the United States and comparison with United States isolates. Avian Dis, 45, 492-499.
  • Cavanagh D (2007): Coronavirus avian infectious bronchitis virus. Vet Res, 38, 281-297.
  • Cook JK, Jackwood M, Jones RC (2012): The long view: 40 years of infectious bronchitis research. Avian Pathol, 41, 239-250.
  • De Wit JJ, Cook, JKA, Van der Heijden HM (2011): Infectious bronchitis virus variants: a review of the history, current situation and control measures. Avian Pathol, 40, 223-235.
  • Feng KY, Chen T, Zhang X, et al (2018): Molecular characteristic and pathogenicity analysis of a virulent recombinant avain infectious bronchitis virus isolated in China. Poult Sci, 97, 3519-3531.
  • Haqshenas G, Assasi K, Akrami H (2005): Isolation and molecular characterization of infectious bronchitis virüs isolate Shiraz IBV, by RT PCR and restriction enzyme analysis. Iran J Vet Res, 6, 70-78.
  • Kahya S, Coven F, Temelli S, et al (2013): Presence of IS/1494/06 genotype-related infectious bronchitis virus in breeder and broiler flocks in Turkey. Ankara Univ Vet Fak Derg, 60, 27-31.
  • King DJ, Cavanagh D (1991): Infectious bronchitis. 471-484. In: BW Calnek, HJ Barnes, CW Beard, WM Reid, HW Yoder (Eds), Diseases of Poultry. Iowa State University Press, Ames.
  • Lai M, Cavanagh D (1997): The molecular biology of coronaviruses. Adv Virus Res, 48, 1-100.
  • Meir R, Rosenblut E, Perl S, et al (2004): Identification of a novel nephropathogenic infectious bronchitis virus in Israel. Avian Dis, 48, 635-641.
  • Valastro V, Holmes EC, Britton P, et al (2016): S1 gene-based phylogeny of infectious bronchitis virus: an attempt to harmonize virus classification. Infect Genet Evol J, 39, 349-364.
  • Yılmaz H, Altan E, Çizmecigil UY, et al (2016): Phylogeny and S1 gene variation of infectious bronchitis virus detected in broilers and layers in Turkey. Avian Dis, 60, 596-602.
  • Yılmaz H, Çizmecigil UY, Gurel AB, et al (2017): Variants of Infectious Bronchitis Virus (Ibv) in chickens in Turkey and production of recombinant Ibv-N protein by using baculovirus expression system. Bulg J Vet Med, 1, 240-247.
There are 15 citations in total.

Details

Primary Language English
Subjects Veterinary Surgery
Journal Section Research Article
Authors

Hasan Öngör 0000-0003-4978-9578

Necati Timurkaan 0000-0001-5567-991X

Fethiye Çöven 0000-0003-1928-7630

Burak Karabulut 0000-0002-4907-6159

Hatice Eröksüz 0000-0002-8407-5792

Burhan Çetinkaya 0000-0001-6347-1684

Tayfun Carlı 0000-0001-6045-8644

Publication Date March 31, 2021
Published in Issue Year 2021Volume: 68 Issue: 2

Cite

APA Öngör, H., Timurkaan, N., Çöven, F., Karabulut, B., et al. (2021). Detection of Israel variant 2 (IS/1494/06) genotype of Infectious Bronchitis Virus in a layer chicken flock. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 68(2), 167-172. https://doi.org/10.33988/auvfd.756970
AMA Öngör H, Timurkaan N, Çöven F, Karabulut B, Eröksüz H, Çetinkaya B, Carlı T. Detection of Israel variant 2 (IS/1494/06) genotype of Infectious Bronchitis Virus in a layer chicken flock. Ankara Univ Vet Fak Derg. March 2021;68(2):167-172. doi:10.33988/auvfd.756970
Chicago Öngör, Hasan, Necati Timurkaan, Fethiye Çöven, Burak Karabulut, Hatice Eröksüz, Burhan Çetinkaya, and Tayfun Carlı. “Detection of Israel Variant 2 (IS/1494/06) Genotype of Infectious Bronchitis Virus in a Layer Chicken Flock”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 68, no. 2 (March 2021): 167-72. https://doi.org/10.33988/auvfd.756970.
EndNote Öngör H, Timurkaan N, Çöven F, Karabulut B, Eröksüz H, Çetinkaya B, Carlı T (March 1, 2021) Detection of Israel variant 2 (IS/1494/06) genotype of Infectious Bronchitis Virus in a layer chicken flock. Ankara Üniversitesi Veteriner Fakültesi Dergisi 68 2 167–172.
IEEE H. Öngör, “Detection of Israel variant 2 (IS/1494/06) genotype of Infectious Bronchitis Virus in a layer chicken flock”, Ankara Univ Vet Fak Derg, vol. 68, no. 2, pp. 167–172, 2021, doi: 10.33988/auvfd.756970.
ISNAD Öngör, Hasan et al. “Detection of Israel Variant 2 (IS/1494/06) Genotype of Infectious Bronchitis Virus in a Layer Chicken Flock”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 68/2 (March 2021), 167-172. https://doi.org/10.33988/auvfd.756970.
JAMA Öngör H, Timurkaan N, Çöven F, Karabulut B, Eröksüz H, Çetinkaya B, Carlı T. Detection of Israel variant 2 (IS/1494/06) genotype of Infectious Bronchitis Virus in a layer chicken flock. Ankara Univ Vet Fak Derg. 2021;68:167–172.
MLA Öngör, Hasan et al. “Detection of Israel Variant 2 (IS/1494/06) Genotype of Infectious Bronchitis Virus in a Layer Chicken Flock”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 68, no. 2, 2021, pp. 167-72, doi:10.33988/auvfd.756970.
Vancouver Öngör H, Timurkaan N, Çöven F, Karabulut B, Eröksüz H, Çetinkaya B, Carlı T. Detection of Israel variant 2 (IS/1494/06) genotype of Infectious Bronchitis Virus in a layer chicken flock. Ankara Univ Vet Fak Derg. 2021;68(2):167-72.