Abstract
References
- Ball C, Awad F, Hutton S, et al (2017): Infectious bronchitis vaccine virus detection and part-S1 genetic variation following single or dual inoculation in broiler chicks. Avian Pathol, 46, 309-318.
- Bassam RH, Hussein HA, Amer MM, et al (2019): Molecular Characterization of VAR2 among IBV Infected poultry in Lebanon. J Virol Sci, 5, 114-123.
- Callison SA, Jackwood MW, Hilt DA (2001): Molecular characterization of infectious bronchitis virus isolates foreign to the United States and comparison with United States isolates. Avian Dis, 45, 492-499.
- Cavanagh D (2007): Coronavirus avian infectious bronchitis virus. Vet Res, 38, 281-297.
- Cook JK, Jackwood M, Jones RC (2012): The long view: 40 years of infectious bronchitis research. Avian Pathol, 41, 239-250.
- De Wit JJ, Cook, JKA, Van der Heijden HM (2011): Infectious bronchitis virus variants: a review of the history, current situation and control measures. Avian Pathol, 40, 223-235.
- Feng KY, Chen T, Zhang X, et al (2018): Molecular characteristic and pathogenicity analysis of a virulent recombinant avain infectious bronchitis virus isolated in China. Poult Sci, 97, 3519-3531.
- Haqshenas G, Assasi K, Akrami H (2005): Isolation and molecular characterization of infectious bronchitis virüs isolate Shiraz IBV, by RT PCR and restriction enzyme analysis. Iran J Vet Res, 6, 70-78.
- Kahya S, Coven F, Temelli S, et al (2013): Presence of IS/1494/06 genotype-related infectious bronchitis virus in breeder and broiler flocks in Turkey. Ankara Univ Vet Fak Derg, 60, 27-31.
- King DJ, Cavanagh D (1991): Infectious bronchitis. 471-484. In: BW Calnek, HJ Barnes, CW Beard, WM Reid, HW Yoder (Eds), Diseases of Poultry. Iowa State University Press, Ames.
- Lai M, Cavanagh D (1997): The molecular biology of coronaviruses. Adv Virus Res, 48, 1-100.
- Meir R, Rosenblut E, Perl S, et al (2004): Identification of a novel nephropathogenic infectious bronchitis virus in Israel. Avian Dis, 48, 635-641.
- Valastro V, Holmes EC, Britton P, et al (2016): S1 gene-based phylogeny of infectious bronchitis virus: an attempt to harmonize virus classification. Infect Genet Evol J, 39, 349-364.
- Yılmaz H, Altan E, Çizmecigil UY, et al (2016): Phylogeny and S1 gene variation of infectious bronchitis virus detected in broilers and layers in Turkey. Avian Dis, 60, 596-602.
- Yılmaz H, Çizmecigil UY, Gurel AB, et al (2017): Variants of Infectious Bronchitis Virus (Ibv) in chickens in Turkey and production of recombinant Ibv-N protein by using baculovirus expression system. Bulg J Vet Med, 1, 240-247.
Detection of Israel variant 2 (IS/1494/06) genotype of Infectious Bronchitis Virus in a layer chicken flock
Abstract
The aim of this study was to determine an infectious bronchitis (IB) infection, caused by an Israel variant 2 (IS/1494/06)-like IBV, in a layer chicken flock regularly vaccinated with vaccines containing IBV H120 and 4/91 strains. Mild respiratory symptoms, drop in egg production and soft-shelled eggs and eventually death were observed in a layer chicken flock. Clinical samples from four diseased chickens were examined for the detection and genotyping of IBV by virus isolation, a commercial real time reverse transcription polymerase chain reaction (rRT-PCR) and nucleotide sequencing. Both Israel variant 2 (IS-Var2) and 793/B serotypes were detected from samples by rRT-PCR, but sequencing results of a 345 bp part of S1 gene revealed that our IBV isolate, HFT-IBV, was IS/1494/06 (IS-Var2)-like with the 97.7% genetic similarity. These results suggested that immunity against vaccination with a combination of different genotypes (H120 and 4/91) could not be protective for IS-Var2 IBV field infection. In addition, identification of genotypes from the clinical samples, such as swabs and organ samples by commercial rRT-PCR assays failed to find correct IBV genotype responsible for the IB infection, whereas analysis of nucleotide sequencing of S1 gene of IBV as a gold standard test undoubtedly detected causative genotype of the infection. Also, the findings indicated that there is an urgent need for consider genotype- or protectotype-match vaccination strategies in the field to prevent vaccine- and IB-dependent economic losses of the poultry sector and logically protect chickens from IBV infection.
References
- Ball C, Awad F, Hutton S, et al (2017): Infectious bronchitis vaccine virus detection and part-S1 genetic variation following single or dual inoculation in broiler chicks. Avian Pathol, 46, 309-318.
- Bassam RH, Hussein HA, Amer MM, et al (2019): Molecular Characterization of VAR2 among IBV Infected poultry in Lebanon. J Virol Sci, 5, 114-123.
- Callison SA, Jackwood MW, Hilt DA (2001): Molecular characterization of infectious bronchitis virus isolates foreign to the United States and comparison with United States isolates. Avian Dis, 45, 492-499.
- Cavanagh D (2007): Coronavirus avian infectious bronchitis virus. Vet Res, 38, 281-297.
- Cook JK, Jackwood M, Jones RC (2012): The long view: 40 years of infectious bronchitis research. Avian Pathol, 41, 239-250.
- De Wit JJ, Cook, JKA, Van der Heijden HM (2011): Infectious bronchitis virus variants: a review of the history, current situation and control measures. Avian Pathol, 40, 223-235.
- Feng KY, Chen T, Zhang X, et al (2018): Molecular characteristic and pathogenicity analysis of a virulent recombinant avain infectious bronchitis virus isolated in China. Poult Sci, 97, 3519-3531.
- Haqshenas G, Assasi K, Akrami H (2005): Isolation and molecular characterization of infectious bronchitis virüs isolate Shiraz IBV, by RT PCR and restriction enzyme analysis. Iran J Vet Res, 6, 70-78.
- Kahya S, Coven F, Temelli S, et al (2013): Presence of IS/1494/06 genotype-related infectious bronchitis virus in breeder and broiler flocks in Turkey. Ankara Univ Vet Fak Derg, 60, 27-31.
- King DJ, Cavanagh D (1991): Infectious bronchitis. 471-484. In: BW Calnek, HJ Barnes, CW Beard, WM Reid, HW Yoder (Eds), Diseases of Poultry. Iowa State University Press, Ames.
- Lai M, Cavanagh D (1997): The molecular biology of coronaviruses. Adv Virus Res, 48, 1-100.
- Meir R, Rosenblut E, Perl S, et al (2004): Identification of a novel nephropathogenic infectious bronchitis virus in Israel. Avian Dis, 48, 635-641.
- Valastro V, Holmes EC, Britton P, et al (2016): S1 gene-based phylogeny of infectious bronchitis virus: an attempt to harmonize virus classification. Infect Genet Evol J, 39, 349-364.
- Yılmaz H, Altan E, Çizmecigil UY, et al (2016): Phylogeny and S1 gene variation of infectious bronchitis virus detected in broilers and layers in Turkey. Avian Dis, 60, 596-602.
- Yılmaz H, Çizmecigil UY, Gurel AB, et al (2017): Variants of Infectious Bronchitis Virus (Ibv) in chickens in Turkey and production of recombinant Ibv-N protein by using baculovirus expression system. Bulg J Vet Med, 1, 240-247.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery |
| Journal Section | Research Article |
| Authors | |
| Publication Date | March 31, 2021 |
| Published in Issue | Year 2021Volume: 68 Issue: 2 |
