In the present study, the prevalence of Salmonella enterica subsp. enterica serovar Infantis (S. Infantis) and other serovars were investigated in samples collected from commercial broiler chicken flocks in Turkey according to the ISO 6579:2002/Amd 1:2007, Annex D, standard method. Furthermore, previously developed S. Infantis-specific polymerase chain reaction (PCR)-based methods with primers targeting fljB, fliC, IMP1-IMP2-IMP3 and sinI were conducted in different media (BPW, MRSV, MKTTN, XLD, and XLT4 agars) and during four incubation stages (6, 12, 18, and 24 h) of the ISO 6579 procedure to develop rapid and reliable diagnosis method. One-hundred thirty-three (15.6%) Salmonella strains were isolated from a total of 848 samples (240 cecal swabs, 200 cloacal swabs, 190 intestinal contents, 59 feed, 39 dust, and 120 litter). The serovar distribution of isolated strains was as follows: S. Infantis, 88.70%; S. Agona, 2.3%; S. Kentucky 1.50, S. Hadar 1.50, and S. Tennessee 1.50; S. Mbandaka 0.75 %, S. Montevideo 0.75 %, S. Enteritidis 0.75 %, S. Adelaide 0.75 %, S. Liverpool 0.75 %, and S. Derby 0.75 %. Primers targeting fljB, fliC, and IMP1-IMP2-IMP3 were not able to detect all S. Infantis isolates, therefore, a novel PCR technique was developed and validated in the study. It was concluded that it is a fast, reproducible and low-cost alternative to the gold standard method by detecting the S. Infantis isolates on the 3rd day at the earliest by PCR (sinI PCR). using primers specific to S. Infantis species.
Primary Language | English |
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Subjects | Veterinary |
Journal Section | Research Article |
Authors |
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Thanks | This study was derived from the PhD thesis of the first author. We would like to thank Prof. Dr. Mehmet Akan (Department of Microbiology, Faculty of Veterinary Medicine, Ankara University, Ankara, Turkey) for his valuable contributions (material maintenance from the field and professional advice on the study) to the study. |
Publication Date | January 1, 2022 |
Published in Issue | Year 2022Volume: 69 Issue: 1 |
Bibtex | @research article { auvfd761263, journal = {Ankara Üniversitesi Veteriner Fakültesi Dergisi}, issn = {1300-0861}, eissn = {1308-2817}, address = {}, publisher = {Ankara University}, year = {2022}, volume = {69}, number = {1}, pages = {1 - 8}, doi = {10.33988/auvfd.761263}, title = {Prevalence and rapid identification of Salmonella Infantis in broiler production in Turkey}, key = {cite}, author = {Şahan Yapıcıer, Özlem and Sareyyüpoğlu, Barış} } |
APA | Şahan Yapıcıer, Ö. & Sareyyüpoğlu, B. (2022). Prevalence and rapid identification of Salmonella Infantis in broiler production in Turkey . Ankara Üniversitesi Veteriner Fakültesi Dergisi , 69 (1) , 1-8 . DOI: 10.33988/auvfd.761263 |
MLA | Şahan Yapıcıer, Ö. , Sareyyüpoğlu, B. "Prevalence and rapid identification of Salmonella Infantis in broiler production in Turkey" . Ankara Üniversitesi Veteriner Fakültesi Dergisi 69 (2022 ): 1-8 <http://vetjournal.ankara.edu.tr/en/pub/issue/67711/761263> |
Chicago | Şahan Yapıcıer, Ö. , Sareyyüpoğlu, B. "Prevalence and rapid identification of Salmonella Infantis in broiler production in Turkey". Ankara Üniversitesi Veteriner Fakültesi Dergisi 69 (2022 ): 1-8 |
RIS | TY - JOUR T1 - Prevalence and rapid identification of Salmonella Infantis in broiler production in Turkey AU - ÖzlemŞahan Yapıcıer, BarışSareyyüpoğlu Y1 - 2022 PY - 2022 N1 - doi: 10.33988/auvfd.761263 DO - 10.33988/auvfd.761263 T2 - Ankara Üniversitesi Veteriner Fakültesi Dergisi JF - Journal JO - JOR SP - 1 EP - 8 VL - 69 IS - 1 SN - 1300-0861-1308-2817 M3 - doi: 10.33988/auvfd.761263 UR - https://doi.org/10.33988/auvfd.761263 Y2 - 2021 ER - |
EndNote | %0 Ankara Üniversitesi Veteriner Fakültesi Dergisi Prevalence and rapid identification of Salmonella Infantis in broiler production in Turkey %A Özlem Şahan Yapıcıer , Barış Sareyyüpoğlu %T Prevalence and rapid identification of Salmonella Infantis in broiler production in Turkey %D 2022 %J Ankara Üniversitesi Veteriner Fakültesi Dergisi %P 1300-0861-1308-2817 %V 69 %N 1 %R doi: 10.33988/auvfd.761263 %U 10.33988/auvfd.761263 |
ISNAD | Şahan Yapıcıer, Özlem , Sareyyüpoğlu, Barış . "Prevalence and rapid identification of Salmonella Infantis in broiler production in Turkey". Ankara Üniversitesi Veteriner Fakültesi Dergisi 69 / 1 (January 2022): 1-8 . https://doi.org/10.33988/auvfd.761263 |
AMA | Şahan Yapıcıer Ö. , Sareyyüpoğlu B. Prevalence and rapid identification of Salmonella Infantis in broiler production in Turkey. Ankara Univ Vet Fak Derg. 2022; 69(1): 1-8. |
Vancouver | Şahan Yapıcıer Ö. , Sareyyüpoğlu B. Prevalence and rapid identification of Salmonella Infantis in broiler production in Turkey. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2022; 69(1): 1-8. |
IEEE | Ö. Şahan Yapıcıer and B. Sareyyüpoğlu , "Prevalence and rapid identification of Salmonella Infantis in broiler production in Turkey", Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 69, no. 1, pp. 1-8, Jan. 2022, doi:10.33988/auvfd.761263 |