Research Article
Wound healing effect of Anzer origin propolis specimens on rats’ intestinal incision
Year 2022, Volume: 69 Issue: 1, 91 - 97, 01.01.2022Abstract
This study was investigated the wound healing effect of Anzer origin propolis specimens on rats’ experimental intestinal incisions. Thirty-four male Wistar albino rats have been divided into five groups, weighing 250-300 gr. Water solution of Anzer propolis (PW3 [n=6], PW7 [n=6]) or ethanolic solution of Anzer propolis (PE3 [n=6], PE7 [n=6]) were administered. Control groups (K3 [n=5], K7 [n=5]) received no treatment. The surgical procedure (day 0) consisted of a laparotomy under general anesthesia, during which the colon was incised and repaired with sutures. The Anzer propolis specimen diluted with physiologic saline was administered by gavage (100 mg/kg/day) for 3 and 7 days before and after surgery to PW3 and PW7 groups. Ethanol dissolved Anzer propolis was administered by gavage (100 mg/kg/day) for 3 and 7 days before and after surgery to PE3 and PE7. Experimental animals have been humanly euthanized on the 3rd and 7th days for biochemical and histopathological evaluations. Serum total oxidant status, total antioxidant status, interleukin-1, interleukin-6, tumor necrosis factor-alpha, myeloperoxidase, hydroxyproline and nitric oxide, levels were measured by ELISA. The incision line sampled to evaluate inflammatory cells, fibroblastic activity, neovascularization, and collagen level. The study indicates that the ethanolic Anzer propolis solution's oral administration has a beneficial effect on intestinal wound healing for seven days pre-and postoperatively (PE7).
Keywords
Anzer Propolis, intestinal incision wound healing, rat, total antioxidant status, total oxidant status
References
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- Akgün A, Kuru S, Uraldi C, et al (2006): Early effects of fibrin sealant on colonic anastomosis in rats: an experimental and case-control study. Tech Coloproctol, 10, 208.
- Alencar SMd, Oldoni TLC, Castro M, et al (2007): Chemical composition and biological activity of a new type of Brazilian propolis: red propolis. J Ethnopharmacol, 113, 278-283.
- Altuğ ME, Serarslan Y, Bal R, et al (2008): Caffeic acid phenethyl ester protects rabbit brains against permanent focal ischemia by antioxidant action: a biochemical and planimetric study. Brain Res J, 1201, 135-142.
- Braskén P (1991): Healing of experimental colon anastomosis. Eur Surg Acta Chirurgica Supplement, 566, 1-51.
- Campo Fernández M, Cuesta-Rubio O, Rosado Perez As, et al (2008): GC-MS determination of isoflavonoids in seven red Cuban propolis samples. J Agric Food Chem, 56, 9927-9932.
- Candiracci M, Piatti E, Dominguez-Barragán M, et al (2012): Anti-inflammatory activity of a honey flavonoid extract on lipopolysaccharide-activated N13 microglial cells. J Agric Food Chem, 60, 12304-12311.
- Carrico TJ, Mehrhof Jr AI, Cohen IK (1984): Biology of wound healing. Surg Clin North Am, 64, 721-733.
- Conner J, Eckersall P, Ferguson J, et al (1988): Acute phase response in the dog following surgical trauma. Res Vet Sci, 45, 107-110.
- Dimov V, Ivanovska N, Manolova N, et al (1991): Immunomodulatory action of propolis. Influence on anti-infectious protection and macrophage function. Apidologie, 22, 155-162.
- Drago L, De Vecchi E, Nicola L, et al (2007): In vitro antimicrobial activity of a novel propolis formulation (Actichelated propolis). J Appl Microbiol, 103, 1914-1921.
- Ertel W, Faist E, Nestle C, et al (1990): Kinetics of interleukin-2 and interleukin-6 synthesis following major mechanical trauma. J Surg Res, 48, 622-628.
- Frostberg E, Ström P, Gerke O, et al (2014): Infliximab’s influence on anastomotic strength and degree of inflammation in intestinal surgery in a rabbit model. BMC Surg, 14, 23.
- Gheldof N, Wang X-H, Engeseth NJ (2002): Identification and quantification of antioxidant components of honeys from various floral sources. J Agric Food Chem, 50, 5870-5877.
- Golub R, Golub RW, Cantu JR, et al (1997): A multivariate analysis of factors contributing to leakage of intestinal anastomoses. J Am Coll Surg, 184, 364-372.
- Graham MF, Drucker DE, Diegelmann RF, et al (1987): Collagen synthesis by human intestinal smooth muscle cells in culture. Gastroenterology, 92, 400-405.
- Gruys E, Toussaint M, Niewold T, et al (2005): Acute phase reaction and acute phase proteins. J Zhejiang Univ Sci B, 6, 1045-1056.
- Gümüş LT, Serinsöz E, Kanik EA, et al (2013): Karın içi adezyon önleyici % 4'lük ikodekstrin solüsyonunun gastrointestinal sistem anastomozları üzerine etkisi. Ulus Travma Acil Cerr Derg, 19, 305-312.
- Harada T, Yoshioka H, Yoshida S, et al (1997): Increased interleukin-6 levels in peritoneal fluid of infertile patients with active endometriosis. Am J Obstet Gynecol, 176, 593-597.
- Heinrich PC, Castell JV, Andus T (1990): Interleukin-6 and the acute phase response. Biochem J, 265, 621-636.
- Kılıçoğlu B, Kılıçoğlu S, Göçen V (2005): Gastrointestinal sistemde yara iyileşmesi. SDÜ Tıp Fak Derg, 12, 67-76.
- Koç O, Dağ A, Öcal AK, et al (2013): Karın içi adezyon önleyici %4’lük İkodekstrin solüsyonunun gastrointestinal sistem üzerine etkisi. Ulus Travma Acil Cerr Derg, 19, 305-312.
- Korkmaz M, Saritas T, Sevimli A, et al (2015): The effect of gabapentin and pregabalin on intestinal incision wound healing in rabbits. Vet World, 8, 279-283.
- Kurek-Górecka A, Rzepecka-Stojko A, Górecki M, et al (2014): Structure and antioxidant activity of polyphenols derived from propolis. Molecules, 19, 78-101.
- Mesaik MA, Dastagir N, Uddin N, et al (2015): Characterization of immunomodulatory activities of honey glycoproteins and glycopeptides. J Agric Food Chem, 63, 177-184.
- Minor R (1980): Collagen metabolism: a comparison of diseases of collagen and diseases affecting collagen. Am J Pathol, 98, 225-280.
- Molan PC, Rhodes T (2015): Honey: A biologic wound dressing. Wounds, 27, 141–151.
- Nishimoto N, Yoshizaki K, Tagoh H, et al (1989): Elevation of serum interleukin 6 prior to acute phase proteins on the inflammation by surgical operation. J Clin Immunol, 50, 399-401.
- Okamato A, Hashimoto K, Nealson KH, et al (2013): Rate enhancement of bacterial extracellular electron transport involves bound flavin semiquiones. Proc Natl Acad Sci, 110, 7856-7861.
- Pepys MB (1981): C-reactive protein fifty years on. The Lancet, 317, 653-657.
- Petretto GL, Cossu M, Alamanni MC (2015): Phenolic content, antioxidant and physico‐chemical properties of Sardinian monofloral honeys. Int J Food Sci Tech, 50, 482-491.
- Pullicino E, Carli F, Poole S, et al (1990): The relationship between the circulating concentrations of interleukin 6 (IL-6), tumor necrosis factor (TNF) and the acute phase response to elective surgery and accidental injury. Lymphokine Res, 9, 231-238.
- Roumen R, Van Meurs P, Kuypers H, et al (1992): Serum interleukin-6 and C reactive protein responses in patients after laparoscopic or conventional cholecystectomy. Eur Surg Acta Chirurgica Supplement, 158, 541-544.
- Shimizu T, Hino A, Tsutsumi A, et al (2008): Anti-influenza virus activity of propolis in vitro and its efficacy against influenza infection in mice. Antivir Chem Chemother, 19, 7-13.
- Silici S, Koç NA, Ayangil D, et al (2005): Antifungal activities of propolis collected by different races of honeybees against yeasts isolated from patients with superficial mycoses. J Pharmacol Sci, 99, 39-44.
- Stadelmann WK, Digenis AG, Tobin GR (1998): Physiology and healing dynamics of chronic cutaneous wounds. Am J Surg, 176, 26S-38S.
- Yağcı MA (2011): Ratlarda Curcumin’in Kolon Anastomozu İyileşmesi Üzerine Etkinliğinin Araştırılması. Yüksek Lisans Tezi. Trakya Üniversitesi Sağlık Bilimleri Enstitüsü, Edirne.
- Yamamoto S, Shida T, Miyaji S, et al (1993): Changes in serum C-reactive protein levels in dogs with various disorders and surgical traumas. Vet Res Commun, 17, 85- 93
Year 2022, Volume: 69 Issue: 1, 91 - 97, 01.01.2022
Abstract
References
- Ahn M-R, Kunimasa K, Ohta T, et al (2007): Suppression of tumor-induced angiogenesis by Brazilian propolis: major component artepillin C inhibits in vitro tube formation and endothelial cell proliferation. Cancer Lett, 252, 235-243.
- Akgün A, Kuru S, Uraldi C, et al (2006): Early effects of fibrin sealant on colonic anastomosis in rats: an experimental and case-control study. Tech Coloproctol, 10, 208.
- Alencar SMd, Oldoni TLC, Castro M, et al (2007): Chemical composition and biological activity of a new type of Brazilian propolis: red propolis. J Ethnopharmacol, 113, 278-283.
- Altuğ ME, Serarslan Y, Bal R, et al (2008): Caffeic acid phenethyl ester protects rabbit brains against permanent focal ischemia by antioxidant action: a biochemical and planimetric study. Brain Res J, 1201, 135-142.
- Braskén P (1991): Healing of experimental colon anastomosis. Eur Surg Acta Chirurgica Supplement, 566, 1-51.
- Campo Fernández M, Cuesta-Rubio O, Rosado Perez As, et al (2008): GC-MS determination of isoflavonoids in seven red Cuban propolis samples. J Agric Food Chem, 56, 9927-9932.
- Candiracci M, Piatti E, Dominguez-Barragán M, et al (2012): Anti-inflammatory activity of a honey flavonoid extract on lipopolysaccharide-activated N13 microglial cells. J Agric Food Chem, 60, 12304-12311.
- Carrico TJ, Mehrhof Jr AI, Cohen IK (1984): Biology of wound healing. Surg Clin North Am, 64, 721-733.
- Conner J, Eckersall P, Ferguson J, et al (1988): Acute phase response in the dog following surgical trauma. Res Vet Sci, 45, 107-110.
- Dimov V, Ivanovska N, Manolova N, et al (1991): Immunomodulatory action of propolis. Influence on anti-infectious protection and macrophage function. Apidologie, 22, 155-162.
- Drago L, De Vecchi E, Nicola L, et al (2007): In vitro antimicrobial activity of a novel propolis formulation (Actichelated propolis). J Appl Microbiol, 103, 1914-1921.
- Ertel W, Faist E, Nestle C, et al (1990): Kinetics of interleukin-2 and interleukin-6 synthesis following major mechanical trauma. J Surg Res, 48, 622-628.
- Frostberg E, Ström P, Gerke O, et al (2014): Infliximab’s influence on anastomotic strength and degree of inflammation in intestinal surgery in a rabbit model. BMC Surg, 14, 23.
- Gheldof N, Wang X-H, Engeseth NJ (2002): Identification and quantification of antioxidant components of honeys from various floral sources. J Agric Food Chem, 50, 5870-5877.
- Golub R, Golub RW, Cantu JR, et al (1997): A multivariate analysis of factors contributing to leakage of intestinal anastomoses. J Am Coll Surg, 184, 364-372.
- Graham MF, Drucker DE, Diegelmann RF, et al (1987): Collagen synthesis by human intestinal smooth muscle cells in culture. Gastroenterology, 92, 400-405.
- Gruys E, Toussaint M, Niewold T, et al (2005): Acute phase reaction and acute phase proteins. J Zhejiang Univ Sci B, 6, 1045-1056.
- Gümüş LT, Serinsöz E, Kanik EA, et al (2013): Karın içi adezyon önleyici % 4'lük ikodekstrin solüsyonunun gastrointestinal sistem anastomozları üzerine etkisi. Ulus Travma Acil Cerr Derg, 19, 305-312.
- Harada T, Yoshioka H, Yoshida S, et al (1997): Increased interleukin-6 levels in peritoneal fluid of infertile patients with active endometriosis. Am J Obstet Gynecol, 176, 593-597.
- Heinrich PC, Castell JV, Andus T (1990): Interleukin-6 and the acute phase response. Biochem J, 265, 621-636.
- Kılıçoğlu B, Kılıçoğlu S, Göçen V (2005): Gastrointestinal sistemde yara iyileşmesi. SDÜ Tıp Fak Derg, 12, 67-76.
- Koç O, Dağ A, Öcal AK, et al (2013): Karın içi adezyon önleyici %4’lük İkodekstrin solüsyonunun gastrointestinal sistem üzerine etkisi. Ulus Travma Acil Cerr Derg, 19, 305-312.
- Korkmaz M, Saritas T, Sevimli A, et al (2015): The effect of gabapentin and pregabalin on intestinal incision wound healing in rabbits. Vet World, 8, 279-283.
- Kurek-Górecka A, Rzepecka-Stojko A, Górecki M, et al (2014): Structure and antioxidant activity of polyphenols derived from propolis. Molecules, 19, 78-101.
- Mesaik MA, Dastagir N, Uddin N, et al (2015): Characterization of immunomodulatory activities of honey glycoproteins and glycopeptides. J Agric Food Chem, 63, 177-184.
- Minor R (1980): Collagen metabolism: a comparison of diseases of collagen and diseases affecting collagen. Am J Pathol, 98, 225-280.
- Molan PC, Rhodes T (2015): Honey: A biologic wound dressing. Wounds, 27, 141–151.
- Nishimoto N, Yoshizaki K, Tagoh H, et al (1989): Elevation of serum interleukin 6 prior to acute phase proteins on the inflammation by surgical operation. J Clin Immunol, 50, 399-401.
- Okamato A, Hashimoto K, Nealson KH, et al (2013): Rate enhancement of bacterial extracellular electron transport involves bound flavin semiquiones. Proc Natl Acad Sci, 110, 7856-7861.
- Pepys MB (1981): C-reactive protein fifty years on. The Lancet, 317, 653-657.
- Petretto GL, Cossu M, Alamanni MC (2015): Phenolic content, antioxidant and physico‐chemical properties of Sardinian monofloral honeys. Int J Food Sci Tech, 50, 482-491.
- Pullicino E, Carli F, Poole S, et al (1990): The relationship between the circulating concentrations of interleukin 6 (IL-6), tumor necrosis factor (TNF) and the acute phase response to elective surgery and accidental injury. Lymphokine Res, 9, 231-238.
- Roumen R, Van Meurs P, Kuypers H, et al (1992): Serum interleukin-6 and C reactive protein responses in patients after laparoscopic or conventional cholecystectomy. Eur Surg Acta Chirurgica Supplement, 158, 541-544.
- Shimizu T, Hino A, Tsutsumi A, et al (2008): Anti-influenza virus activity of propolis in vitro and its efficacy against influenza infection in mice. Antivir Chem Chemother, 19, 7-13.
- Silici S, Koç NA, Ayangil D, et al (2005): Antifungal activities of propolis collected by different races of honeybees against yeasts isolated from patients with superficial mycoses. J Pharmacol Sci, 99, 39-44.
- Stadelmann WK, Digenis AG, Tobin GR (1998): Physiology and healing dynamics of chronic cutaneous wounds. Am J Surg, 176, 26S-38S.
- Yağcı MA (2011): Ratlarda Curcumin’in Kolon Anastomozu İyileşmesi Üzerine Etkinliğinin Araştırılması. Yüksek Lisans Tezi. Trakya Üniversitesi Sağlık Bilimleri Enstitüsü, Edirne.
- Yamamoto S, Shida T, Miyaji S, et al (1993): Changes in serum C-reactive protein levels in dogs with various disorders and surgical traumas. Vet Res Commun, 17, 85- 93
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary |
| Journal Section | Research Article |
| Authors |
|
| Supporting Institution | AKÜ BAPK |
| Project Number | AKÜ BAPK 18.Kariyer.242 |
| Publication Date | January 1, 2022 |
| Published in Issue | Year 2022Volume: 69 Issue: 1 |
Cite
| Bibtex | @research article { auvfd834703, journal = {Ankara Üniversitesi Veteriner Fakültesi Dergisi}, issn = {1300-0861}, eissn = {1308-2817}, address = {}, publisher = {Ankara University}, year = {2022}, volume = {69}, number = {1}, pages = {91 - 97}, doi = {10.33988/auvfd.834703}, title = {Wound healing effect of Anzer origin propolis specimens on rats’ intestinal incision}, key = {cite}, author = {Sarıtaş, Zülfükar and Korkmaz, Musa and Demirel, Hasan Hüseyin and Bülbül, Aziz and Sarıtaş, Tuba Berra and Görücü, Fatma and Koç, Yusuf} } |
| APA | Sarıtaş, Z. , Korkmaz, M. , Demirel, H. H. , Bülbül, A. , Sarıtaş, T. B. , Görücü, F. & Koç, Y. (2022). Wound healing effect of Anzer origin propolis specimens on rats’ intestinal incision . Ankara Üniversitesi Veteriner Fakültesi Dergisi , 69 (1) , 91-97 . DOI: 10.33988/auvfd.834703 |
| MLA | Sarıtaş, Z. , Korkmaz, M. , Demirel, H. H. , Bülbül, A. , Sarıtaş, T. B. , Görücü, F. , Koç, Y. "Wound healing effect of Anzer origin propolis specimens on rats’ intestinal incision" . Ankara Üniversitesi Veteriner Fakültesi Dergisi 69 (2022 ): 91-97 <http://vetjournal.ankara.edu.tr/en/pub/issue/67711/834703> |
| Chicago | Sarıtaş, Z. , Korkmaz, M. , Demirel, H. H. , Bülbül, A. , Sarıtaş, T. B. , Görücü, F. , Koç, Y. "Wound healing effect of Anzer origin propolis specimens on rats’ intestinal incision". Ankara Üniversitesi Veteriner Fakültesi Dergisi 69 (2022 ): 91-97 |
| RIS | TY - JOUR T1 - Wound healing effect of Anzer origin propolis specimens on rats’ intestinal incision AU - ZülfükarSarıtaş, MusaKorkmaz, Hasan HüseyinDemirel, AzizBülbül, Tuba BerraSarıtaş, FatmaGörücü, YusufKoç Y1 - 2022 PY - 2022 N1 - doi: 10.33988/auvfd.834703 DO - 10.33988/auvfd.834703 T2 - Ankara Üniversitesi Veteriner Fakültesi Dergisi JF - Journal JO - JOR SP - 91 EP - 97 VL - 69 IS - 1 SN - 1300-0861-1308-2817 M3 - doi: 10.33988/auvfd.834703 UR - https://doi.org/10.33988/auvfd.834703 Y2 - 2021 ER - |
| EndNote | %0 Ankara Üniversitesi Veteriner Fakültesi Dergisi Wound healing effect of Anzer origin propolis specimens on rats’ intestinal incision %A Zülfükar Sarıtaş , Musa Korkmaz , Hasan Hüseyin Demirel , Aziz Bülbül , Tuba Berra Sarıtaş , Fatma Görücü , Yusuf Koç %T Wound healing effect of Anzer origin propolis specimens on rats’ intestinal incision %D 2022 %J Ankara Üniversitesi Veteriner Fakültesi Dergisi %P 1300-0861-1308-2817 %V 69 %N 1 %R doi: 10.33988/auvfd.834703 %U 10.33988/auvfd.834703 |
| ISNAD | Sarıtaş, Zülfükar , Korkmaz, Musa , Demirel, Hasan Hüseyin , Bülbül, Aziz , Sarıtaş, Tuba Berra , Görücü, Fatma , Koç, Yusuf . "Wound healing effect of Anzer origin propolis specimens on rats’ intestinal incision". Ankara Üniversitesi Veteriner Fakültesi Dergisi 69 / 1 (January 2022): 91-97 . https://doi.org/10.33988/auvfd.834703 |
| AMA | Sarıtaş Z. , Korkmaz M. , Demirel H. H. , Bülbül A. , Sarıtaş T. B. , Görücü F. , Koç Y. Wound healing effect of Anzer origin propolis specimens on rats’ intestinal incision. Ankara Univ Vet Fak Derg. 2022; 69(1): 91-97. |
| Vancouver | Sarıtaş Z. , Korkmaz M. , Demirel H. H. , Bülbül A. , Sarıtaş T. B. , Görücü F. , Koç Y. Wound healing effect of Anzer origin propolis specimens on rats’ intestinal incision. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2022; 69(1): 91-97. |
| IEEE | Z. Sarıtaş , M. Korkmaz , H. H. Demirel , A. Bülbül , T. B. Sarıtaş , F. Görücü and Y. Koç , "Wound healing effect of Anzer origin propolis specimens on rats’ intestinal incision", Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 69, no. 1, pp. 91-97, Jan. 2022, doi:10.33988/auvfd.834703 |
