Nitrite and amine reactions can occur rapidly and produce nitrosamines, in-vivo. Diethylnitrosamine (DEN) and phenobarbital (PB) are readily inducing liver injury and hesperetin (HES), as a flavonoid found in citrus fruits, have the potential to compensate for their harmful effects. In this study, the therapeutic effects of HES were evaluated in DEN and PB mediated liver defect. Adult male Sprague-Dawley rats were split into 5 groups (n=10): Control, DEN, DEN+PB, HES, and DEN+PB+HES. 150 mg/kg DEN was applied intraperitoneally to DEN groups. Fifteen days after the DEN application 500ppm of PB was given in drinking water. HES were administered at 50 mg/kg dose orally for 8 weeks. Blood and liver malondialdehyde (MDA), glutathione (GSH) levels, and catalase (CAT), superoxide dismutase (SOD) activities were measured spectrophotometrically. Moreover, histologic examination of liver sections and apoptosis were determined with hematoxylin-eosin and TUNEL methods, respectively. DEN-PB application was found to increase blood and liver MDA levels and liver CAT activity, oppositely, decreased blood and liver SOD activity, GSH levels, and blood CAT activity. HES was found to have a positive impact on oxidative stress parameters by decreasing liver and blood MDA activity, increasing blood CAT and SOD activity together with liver GSH levels and SOD activity. Whereas DEN and PB application increased all histopathological findings and TUNEL positive cells, HES administration decreased these findings which might be important for the protection of liver cell structure from cell damage. These results suggest that HES administration could be an alternative therapeutic approach to liver damage.
Apoptosis, Diethylnitrosamine, Hesperetin, Oxidative stress, phenobarbital
Primary Language | English |
---|---|
Subjects | Veterinary |
Journal Section | Research Article |
Authors |
|
Supporting Institution | This research was supported by a grant supplied from “Firat University Research Fund” (VF.16.22). |
Project Number | VF.16.22 |
Thanks | This paper presented in International Conference on Multidisciplinary, Engineering, Science, Education and Technology, 2018, Dubai. |
Publication Date | March 25, 2022 |
Published in Issue | Year 2022Volume: 69 Issue: 2 |
Bibtex | @research article { auvfd812718, journal = {Ankara Üniversitesi Veteriner Fakültesi Dergisi}, issn = {1300-0861}, eissn = {1308-2817}, address = {}, publisher = {Ankara University}, year = {2022}, volume = {69}, number = {2}, pages = {149 - 156}, doi = {10.33988/auvfd.812718}, title = {The evaluation of the therapeutic potential of hesperetin on diethylnitrosamine and phenobarbital induced liver injury in rats}, key = {cite}, author = {Kısaçam, Mehmet Ali and Ozan Kocamüftüoğlu, Gonca and Kaya Tektemur, Nalan and Temizer Ozan, Penbe Sema} } |
APA | Kısaçam, M. A. , Ozan Kocamüftüoğlu, G. , Kaya Tektemur, N. & Temizer Ozan, P. S. (2022). The evaluation of the therapeutic potential of hesperetin on diethylnitrosamine and phenobarbital induced liver injury in rats . Ankara Üniversitesi Veteriner Fakültesi Dergisi , 69 (2) , 149-156 . DOI: 10.33988/auvfd.812718 |
MLA | Kısaçam, M. A. , Ozan Kocamüftüoğlu, G. , Kaya Tektemur, N. , Temizer Ozan, P. S. "The evaluation of the therapeutic potential of hesperetin on diethylnitrosamine and phenobarbital induced liver injury in rats" . Ankara Üniversitesi Veteriner Fakültesi Dergisi 69 (2022 ): 149-156 <http://vetjournal.ankara.edu.tr/en/pub/issue/69048/812718> |
Chicago | Kısaçam, M. A. , Ozan Kocamüftüoğlu, G. , Kaya Tektemur, N. , Temizer Ozan, P. S. "The evaluation of the therapeutic potential of hesperetin on diethylnitrosamine and phenobarbital induced liver injury in rats". Ankara Üniversitesi Veteriner Fakültesi Dergisi 69 (2022 ): 149-156 |
RIS | TY - JOUR T1 - The evaluation of the therapeutic potential of hesperetin on diethylnitrosamine and phenobarbital induced liver injury in rats AU - Mehmet AliKısaçam, GoncaOzan Kocamüftüoğlu, NalanKaya Tektemur, Penbe SemaTemizer Ozan Y1 - 2022 PY - 2022 N1 - doi: 10.33988/auvfd.812718 DO - 10.33988/auvfd.812718 T2 - Ankara Üniversitesi Veteriner Fakültesi Dergisi JF - Journal JO - JOR SP - 149 EP - 156 VL - 69 IS - 2 SN - 1300-0861-1308-2817 M3 - doi: 10.33988/auvfd.812718 UR - https://doi.org/10.33988/auvfd.812718 Y2 - 2021 ER - |
EndNote | %0 Ankara Üniversitesi Veteriner Fakültesi Dergisi The evaluation of the therapeutic potential of hesperetin on diethylnitrosamine and phenobarbital induced liver injury in rats %A Mehmet Ali Kısaçam , Gonca Ozan Kocamüftüoğlu , Nalan Kaya Tektemur , Penbe Sema Temizer Ozan %T The evaluation of the therapeutic potential of hesperetin on diethylnitrosamine and phenobarbital induced liver injury in rats %D 2022 %J Ankara Üniversitesi Veteriner Fakültesi Dergisi %P 1300-0861-1308-2817 %V 69 %N 2 %R doi: 10.33988/auvfd.812718 %U 10.33988/auvfd.812718 |
ISNAD | Kısaçam, Mehmet Ali , Ozan Kocamüftüoğlu, Gonca , Kaya Tektemur, Nalan , Temizer Ozan, Penbe Sema . "The evaluation of the therapeutic potential of hesperetin on diethylnitrosamine and phenobarbital induced liver injury in rats". Ankara Üniversitesi Veteriner Fakültesi Dergisi 69 / 2 (March 2022): 149-156 . https://doi.org/10.33988/auvfd.812718 |
AMA | Kısaçam M. A. , Ozan Kocamüftüoğlu G. , Kaya Tektemur N. , Temizer Ozan P. S. The evaluation of the therapeutic potential of hesperetin on diethylnitrosamine and phenobarbital induced liver injury in rats. Ankara Univ Vet Fak Derg. 2022; 69(2): 149-156. |
Vancouver | Kısaçam M. A. , Ozan Kocamüftüoğlu G. , Kaya Tektemur N. , Temizer Ozan P. S. The evaluation of the therapeutic potential of hesperetin on diethylnitrosamine and phenobarbital induced liver injury in rats. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2022; 69(2): 149-156. |
IEEE | M. A. Kısaçam , G. Ozan Kocamüftüoğlu , N. Kaya Tektemur and P. S. Temizer Ozan , "The evaluation of the therapeutic potential of hesperetin on diethylnitrosamine and phenobarbital induced liver injury in rats", Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 69, no. 2, pp. 149-156, Mar. 2022, doi:10.33988/auvfd.812718 |