The potential protective effects of mesenchymal stem cells (MSCs) on some kidney diseases have been reported. However, the effect of the fetal kidney–derived (FKD)MSCs on doxorubicin-induced nephropathy has not been studied yet. This study aimed to treat rats with doxorubicin-induced kidney injuries by transplantation of –FKD-MSCs. Twenty-four Sprague-Dawley rats were divided into three groups as control, doxorubicin nephropathy (Sham), and doxorubicin + MSC treated group. Serum biochemistry analysis was performed at the beginning and the end of the study. Functional changes in kidneys were evaluated by scintigraphy. In the doxorubicin nephropathy group, histopathological findings such as mesangial cell proliferation, tubular cast, and glomerular hypertrophy were observed, whereas in the MSC group these findings were significantly reduced. CD133 and CD24 positive immunoreactions were the most severe and frequently observed in the MSC group. While positive staining was detected in the tubular epithelium, there was no immunostaining observed in the glomerulus. The results showed that both functional and histological improvements were achieved in the MSC group compared to the Sham group. In conclusion, transplantation of fetal kidney - derived MSCs into patients with renal damage is thought to contribute to the healing of the renal tissue.
Mezenkimal kök hücrelerin (MKH) bazı böbrek hastalıklarındaki potansiyel koruyucu etkileri bildirilmiştir. Bununla birlikte, fetal böbrek kaynaklı MKH'ların doksorubisin ile indüklenmiş nefropati üzerindeki etkisi henüz araştırılmamıştır. Bu çalışmanın amacı, doksorubisin kaynaklı böbrek hasarı olan ratlara fetal böbrek kaynaklı MKH'ların transplantasyonu yapılarak tedavi edilmesidir. Çalışmada yirmi dört adet Sprague – Dawley ırkı rat üç gruba ayrılmıştır. Bunlar: kontrol grubu, doksorubisin nefropatisi (Sham) grubu ve doksorubisin + MKH ile tedavi edilen gruptur. Çalışmanın başında ve sonunda ratlardan kan alınarak serum biyokimya çalışılmıştır. Böbreklerdeki fonksiyonel değişiklikler sintigrafi ile değerlendirilmiştir. Doksorubisin nefropatisi grubunda mezanjiyal hücre proliferasyonu, tübüler alçı ve glomerüler hipertrofi gibi histopatolojik bulgular gözlenirken, MKH grubunda bu bulgular anlamlı olarak azalmıştır. CD133 ve CD24 pozitif immünreaksiyonlar, en şiddetli ve en sık olarak MKH grubunda gözlenmiştir. Tübüler epitelde pozitif boyanma tespit edilirken glomerulusta immün boyanma gözlenmemiştir. Sonuçlar, Sham grubuna kıyasla MKH grubunda hem fonksiyonel hem de histolojik iyileşmelerin sağlandığını göstermiştir. Sonuç olarak, böbrek hasarı olan hastalara fetal böbrek kaynaklı MKH transplantasyonunun böbrek dokusunun iyileşmesine katkıda bulunduğu düşünülmektedir.
Primary Language | English |
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Subjects | Veterinary |
Journal Section | Research Article |
Authors |
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Supporting Institution | This work was supported by Aksaray University Scientific Research Fund (Grant number 2015 – 065). |
Project Number | Grant number 2015 – 065 |
Publication Date | March 25, 2022 |
Published in Issue | Year 2022Volume: 69 Issue: 2 |
Bibtex | @research article { auvfd822776, journal = {Ankara Üniversitesi Veteriner Fakültesi Dergisi}, issn = {1300-0861}, eissn = {1308-2817}, address = {}, publisher = {Ankara University}, year = {2022}, volume = {69}, number = {2}, pages = {201 - 209}, doi = {10.33988/auvfd.822776}, title = {Investigation of the effects of fetal rat kidney-derived mesenchymal stem cells implementation on doxorubicin-induced nephropathy in male Sprague–Dawley rats}, key = {cite}, author = {Boztok Özgermen, Başak and Bulut, Gaye and Alpaslan Pınarlı, Ferda and Gültekin, Salih Sinan and Özen, Doğukan and Yavuz, Orhan and Haydardedeoğlu, Ali Evren} } |
APA | Boztok Özgermen, B. , Bulut, G. , Alpaslan Pınarlı, F. , Gültekin, S. S. , Özen, D. , Yavuz, O. & Haydardedeoğlu, A. E. (2022). Investigation of the effects of fetal rat kidney-derived mesenchymal stem cells implementation on doxorubicin-induced nephropathy in male Sprague–Dawley rats . Ankara Üniversitesi Veteriner Fakültesi Dergisi , 69 (2) , 201-209 . DOI: 10.33988/auvfd.822776 |
MLA | Boztok Özgermen, B. , Bulut, G. , Alpaslan Pınarlı, F. , Gültekin, S. S. , Özen, D. , Yavuz, O. , Haydardedeoğlu, A. E. "Investigation of the effects of fetal rat kidney-derived mesenchymal stem cells implementation on doxorubicin-induced nephropathy in male Sprague–Dawley rats" . Ankara Üniversitesi Veteriner Fakültesi Dergisi 69 (2022 ): 201-209 <http://vetjournal.ankara.edu.tr/en/pub/issue/69048/822776> |
Chicago | Boztok Özgermen, B. , Bulut, G. , Alpaslan Pınarlı, F. , Gültekin, S. S. , Özen, D. , Yavuz, O. , Haydardedeoğlu, A. E. "Investigation of the effects of fetal rat kidney-derived mesenchymal stem cells implementation on doxorubicin-induced nephropathy in male Sprague–Dawley rats". Ankara Üniversitesi Veteriner Fakültesi Dergisi 69 (2022 ): 201-209 |
RIS | TY - JOUR T1 - Investigation of the effects of fetal rat kidney-derived mesenchymal stem cells implementation on doxorubicin-induced nephropathy in male Sprague–Dawley rats AU - BaşakBoztok Özgermen, GayeBulut, FerdaAlpaslan Pınarlı, Salih SinanGültekin, DoğukanÖzen, OrhanYavuz, Ali EvrenHaydardedeoğlu Y1 - 2022 PY - 2022 N1 - doi: 10.33988/auvfd.822776 DO - 10.33988/auvfd.822776 T2 - Ankara Üniversitesi Veteriner Fakültesi Dergisi JF - Journal JO - JOR SP - 201 EP - 209 VL - 69 IS - 2 SN - 1300-0861-1308-2817 M3 - doi: 10.33988/auvfd.822776 UR - https://doi.org/10.33988/auvfd.822776 Y2 - 2021 ER - |
EndNote | %0 Ankara Üniversitesi Veteriner Fakültesi Dergisi Investigation of the effects of fetal rat kidney-derived mesenchymal stem cells implementation on doxorubicin-induced nephropathy in male Sprague–Dawley rats %A Başak Boztok Özgermen , Gaye Bulut , Ferda Alpaslan Pınarlı , Salih Sinan Gültekin , Doğukan Özen , Orhan Yavuz , Ali Evren Haydardedeoğlu %T Investigation of the effects of fetal rat kidney-derived mesenchymal stem cells implementation on doxorubicin-induced nephropathy in male Sprague–Dawley rats %D 2022 %J Ankara Üniversitesi Veteriner Fakültesi Dergisi %P 1300-0861-1308-2817 %V 69 %N 2 %R doi: 10.33988/auvfd.822776 %U 10.33988/auvfd.822776 |
ISNAD | Boztok Özgermen, Başak , Bulut, Gaye , Alpaslan Pınarlı, Ferda , Gültekin, Salih Sinan , Özen, Doğukan , Yavuz, Orhan , Haydardedeoğlu, Ali Evren . "Investigation of the effects of fetal rat kidney-derived mesenchymal stem cells implementation on doxorubicin-induced nephropathy in male Sprague–Dawley rats". Ankara Üniversitesi Veteriner Fakültesi Dergisi 69 / 2 (March 2022): 201-209 . https://doi.org/10.33988/auvfd.822776 |
AMA | Boztok Özgermen B. , Bulut G. , Alpaslan Pınarlı F. , Gültekin S. S. , Özen D. , Yavuz O. , Haydardedeoğlu A. E. Investigation of the effects of fetal rat kidney-derived mesenchymal stem cells implementation on doxorubicin-induced nephropathy in male Sprague–Dawley rats. Ankara Univ Vet Fak Derg. 2022; 69(2): 201-209. |
Vancouver | Boztok Özgermen B. , Bulut G. , Alpaslan Pınarlı F. , Gültekin S. S. , Özen D. , Yavuz O. , Haydardedeoğlu A. E. Investigation of the effects of fetal rat kidney-derived mesenchymal stem cells implementation on doxorubicin-induced nephropathy in male Sprague–Dawley rats. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2022; 69(2): 201-209. |
IEEE | B. Boztok Özgermen , G. Bulut , F. Alpaslan Pınarlı , S. S. Gültekin , D. Özen , O. Yavuz and A. E. Haydardedeoğlu , "Investigation of the effects of fetal rat kidney-derived mesenchymal stem cells implementation on doxorubicin-induced nephropathy in male Sprague–Dawley rats", Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 69, no. 2, pp. 201-209, Mar. 2022, doi:10.33988/auvfd.822776 |