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Conventional and molecular identification of Brucella isolates from livestock in Turkey

Year 2022, Volume: 69 Issue: 3, 297 - 302, 30.06.2022
https://doi.org/10.33988/auvfd.796785

Abstract

The main object of the study is to demonstrate that Bruce-ladder multiplex PCR, which is capable of identify all Brucella species including vaccinal strains, is a safe and practical method that is alternative to bacteriological culture methods. Brucellosis is a zoonosis of great socio-economic importance which is endemic in Turkey. In three-year period from 2009 to 2011, a total of 281 Brucella spp. isolates from cattle, sheep and goats were identified and characterized by both conventional biotyping procedures and the PCR based method called Bruce-ladder. Species identification from both phenotypic testing and Bruce-ladder was concordant for all isolates tested. The results showed preferred host pattern in B.abortus isolates. In terms of species identity, it was found that 94% of the isolations derived from cattle were B.abortus and were exclusively biotype 3. B.melitensis biotype 3 and biotype 1 were the prevelant biotypes in sheep and goats. Two isolates from sheep were identified as B.melitensis Rev.1 vaccine strain. Surprisingly, one of the goat isolates was identified as B.abortus S19 vaccine strain. Other interesting observations were the isolation of penicilin-sensitive non vaccine strains of B.abortus and B.melitensis as well as an isolation of an atypical B.abortus biotype 3 strain on the basis of growth inhibition in the presence of basic fuchsin dye. At the end of this study, it was concluded that monitoring Brucella isolates and identify them are indispensable epidemiological tool for brucellosis control

Thanks

Part of this study has been presented in Brucellosis 2011 International Research Conference, Including 64th Research Conference, 21-23 September, Buenos Aires, Argentina.

References

  • Aldomy FMM, Jahans KL, Altarzi YH (1992): Isolation of Brucella melitensis from aborting ruminants in Jordan. J Comp Path, 107, 239-242.
  • Alton GG, Jones LM, Angus RD, et al (1988): Techniques for the Brucellosis Laboratory. INRA, Paris, France.
  • Banai M, Mayer I, Cohen A (1990): Isolation, identification, and characterization in Israel of Brucella melitensis biovar 1 atypical strains susceptible to dyes and penicillin, indicating the evolution of a new variant. J Clin Microbiol, 28, 1057-1059.
  • Blasco JM (1997): A review of the use of B.melitensis Rev 1 vaccine in adult sheep and goats. Prev Vet Med, 31, 275-283.
  • Bricker BJ, Halling SM (1994): Differentiation of Brucella abortus bv. 1,2, and 4, Brucella melitensis, Brucella ovis, and Brucella suis bv. 1 by PCR. J Clin Microbiol, 32, 2660-2666.
  • Büyükçangaz E, Şen A, Kahya S (2009): Isolation and biotyping of Brucella melitensis from aborted sheep and goat fetuses. Turk J Vet Anim Sci, 33, 311-316.
  • Corbel MJ (1989): Brucellosis: epidemiology and prevalence worldwide. 25-40. In: Young EJ, Corbel MJ (Ed), Brucellosis: Clinical and Laboratory Aspects. CRC Press 1 st ed., Boca Raton, FL, USA.
  • Corbel MJ (1989): Microbiology of the genus Brucella. 54-67. In: Young EJ, Corbel MJ (Ed), Brucellosis: Clinical and Laboratory Aspects. CRC Press 1st ed., Boca Raton, FL, USA.
  • Corbel MJ (1991): Identification of dye-sensitive strains of Brucella melitensis. J Clin Microbiol, 29, 1066-1068.
  • Corbel MJ (1997): Brucellosis:an overwiev. Emerg Infect Dis, 3, 213-221.
  • Erdenlig S, Şen A (2000): Koyun atıklarından izole edilen Brucella cinsi mikroorganizmaların izolasyonu ve biyotiplendirilmesi. Pendik Vet Mikrobiol Derg, 31, 31-42.
  • Erdenlig S, Iyisan AS, Baklan EA, et al (2007): Biovar distrubution of Brucella isolates from livestock in Turkey, 1999 to 2006. 27-28. In: Proceedings of the 15th International Congress of Mediterrenean Federation for Health and Production of Ruminants. Kuşadası, Turkey.
  • Ewalt DR, Forbes LB (1987): Atypical isolates of Brucella abortus from Canada and the United States characterized as dye sensitive with M antigen dominant. J Clin Microbiol, 25, 698-701.
  • Foster G, Osterman BS, Godfroid J, et al (2007): Brucella ceti sp. nov. Brucella pinnipedialis sp. nov. for Brucella strains with cetaceans and seals as their preferred hosts. Int J Syst Evol Microbiol, 57, 2688–2693.
  • Garcia-Yoldi D, Marin CM, De Miguel MJ, et al (2006): Multiplex PCR assay for the identification and differentiation of all Brucella species and the vaccine strains Brucella abortus S19 and RB51 and Brucella melitensis Rev1. Clin Chem, 52, 779–781.
  • Güler L, Gündüz K, Ok U (2003): Comparison of polymerase chain reaction and bacteriological culture for the diagnosis of sheep brucellosis using aborted fetus samples. Vet Microbiol, 93, 53-61.
  • Hemashettar BM, Patil CS, Jayakumar K, et al (1987): Isolation of Brucella melitensis biotype 1 from a cow and two of its attenders. Indian Vet J, 64, 822-825.
  • Ica T, Aydın F, Erdenliğ S, et al (2008): Characterisation of Brucella abortus biovar 3 isolates from Turkey as biovar 3b. The Vet Rec, 29, 660-662.
  • Manual of Diagnostic Tests and Vaccines for Terrestrial Animals (2019): Chapter 3.1.4. Brucellosis (Brucella abortus, B. melitensis and B. suis) (infection with B. abortus, B. melitensis and B. suis) (NB: Version adopted in May2016), Available at https://www.oie.int/fileadmin/ Home/eng/Health_standards/tahm/3.01.04_BRUCELLOSIS.pdf; [Accessed August 19, 2020].
  • Mayer-Scholl A, Draeger A, Göllner C, et al (2010): Advancement of a multiplex PCR for the differentiation of all currently described Brucella species. J Microbiol Method, 80, 112-114.
  • Ocholi RA, Kwaga JKP, Ajogi I, et al (2005): Abortion due to Brucella abortus in sheep in Nigeria. Rev Sci Tech Int Epiz, 24, 973-979.
  • Saytekin AM, Ak S (2018): Direct diagnosis of Brucella species through multiplex PCR formed by a new method. J Microbiol Method, 154, 86-94.
  • Scholz HC, Hubalek Z, Sedlacek I, et al (2008): Brucella microti sp. nov., isolated from the commonvole Microtus arvalis. Int J Syst Evol Microbiol, 58, 375–382.
  • Shaw WB (1976): Brucella abortus infection in sheep. I. Field case. Br Vet J, 132, 18-26.
  • Stinebring WR, Kunkel JR (1982): In vitro susceptibility of selected isolates of Brucella abortus to penicilin. Am J Vet Res, 43, 545-547.
  • Şahin M, Genç O, Ünver A, et al (2008): Investigation of bovine brucellosis in the Northeastern Turkey. Trop Anim Health Product, 40, 281-286.
Year 2022, Volume: 69 Issue: 3, 297 - 302, 30.06.2022
https://doi.org/10.33988/auvfd.796785

Abstract

References

  • Aldomy FMM, Jahans KL, Altarzi YH (1992): Isolation of Brucella melitensis from aborting ruminants in Jordan. J Comp Path, 107, 239-242.
  • Alton GG, Jones LM, Angus RD, et al (1988): Techniques for the Brucellosis Laboratory. INRA, Paris, France.
  • Banai M, Mayer I, Cohen A (1990): Isolation, identification, and characterization in Israel of Brucella melitensis biovar 1 atypical strains susceptible to dyes and penicillin, indicating the evolution of a new variant. J Clin Microbiol, 28, 1057-1059.
  • Blasco JM (1997): A review of the use of B.melitensis Rev 1 vaccine in adult sheep and goats. Prev Vet Med, 31, 275-283.
  • Bricker BJ, Halling SM (1994): Differentiation of Brucella abortus bv. 1,2, and 4, Brucella melitensis, Brucella ovis, and Brucella suis bv. 1 by PCR. J Clin Microbiol, 32, 2660-2666.
  • Büyükçangaz E, Şen A, Kahya S (2009): Isolation and biotyping of Brucella melitensis from aborted sheep and goat fetuses. Turk J Vet Anim Sci, 33, 311-316.
  • Corbel MJ (1989): Brucellosis: epidemiology and prevalence worldwide. 25-40. In: Young EJ, Corbel MJ (Ed), Brucellosis: Clinical and Laboratory Aspects. CRC Press 1 st ed., Boca Raton, FL, USA.
  • Corbel MJ (1989): Microbiology of the genus Brucella. 54-67. In: Young EJ, Corbel MJ (Ed), Brucellosis: Clinical and Laboratory Aspects. CRC Press 1st ed., Boca Raton, FL, USA.
  • Corbel MJ (1991): Identification of dye-sensitive strains of Brucella melitensis. J Clin Microbiol, 29, 1066-1068.
  • Corbel MJ (1997): Brucellosis:an overwiev. Emerg Infect Dis, 3, 213-221.
  • Erdenlig S, Şen A (2000): Koyun atıklarından izole edilen Brucella cinsi mikroorganizmaların izolasyonu ve biyotiplendirilmesi. Pendik Vet Mikrobiol Derg, 31, 31-42.
  • Erdenlig S, Iyisan AS, Baklan EA, et al (2007): Biovar distrubution of Brucella isolates from livestock in Turkey, 1999 to 2006. 27-28. In: Proceedings of the 15th International Congress of Mediterrenean Federation for Health and Production of Ruminants. Kuşadası, Turkey.
  • Ewalt DR, Forbes LB (1987): Atypical isolates of Brucella abortus from Canada and the United States characterized as dye sensitive with M antigen dominant. J Clin Microbiol, 25, 698-701.
  • Foster G, Osterman BS, Godfroid J, et al (2007): Brucella ceti sp. nov. Brucella pinnipedialis sp. nov. for Brucella strains with cetaceans and seals as their preferred hosts. Int J Syst Evol Microbiol, 57, 2688–2693.
  • Garcia-Yoldi D, Marin CM, De Miguel MJ, et al (2006): Multiplex PCR assay for the identification and differentiation of all Brucella species and the vaccine strains Brucella abortus S19 and RB51 and Brucella melitensis Rev1. Clin Chem, 52, 779–781.
  • Güler L, Gündüz K, Ok U (2003): Comparison of polymerase chain reaction and bacteriological culture for the diagnosis of sheep brucellosis using aborted fetus samples. Vet Microbiol, 93, 53-61.
  • Hemashettar BM, Patil CS, Jayakumar K, et al (1987): Isolation of Brucella melitensis biotype 1 from a cow and two of its attenders. Indian Vet J, 64, 822-825.
  • Ica T, Aydın F, Erdenliğ S, et al (2008): Characterisation of Brucella abortus biovar 3 isolates from Turkey as biovar 3b. The Vet Rec, 29, 660-662.
  • Manual of Diagnostic Tests and Vaccines for Terrestrial Animals (2019): Chapter 3.1.4. Brucellosis (Brucella abortus, B. melitensis and B. suis) (infection with B. abortus, B. melitensis and B. suis) (NB: Version adopted in May2016), Available at https://www.oie.int/fileadmin/ Home/eng/Health_standards/tahm/3.01.04_BRUCELLOSIS.pdf; [Accessed August 19, 2020].
  • Mayer-Scholl A, Draeger A, Göllner C, et al (2010): Advancement of a multiplex PCR for the differentiation of all currently described Brucella species. J Microbiol Method, 80, 112-114.
  • Ocholi RA, Kwaga JKP, Ajogi I, et al (2005): Abortion due to Brucella abortus in sheep in Nigeria. Rev Sci Tech Int Epiz, 24, 973-979.
  • Saytekin AM, Ak S (2018): Direct diagnosis of Brucella species through multiplex PCR formed by a new method. J Microbiol Method, 154, 86-94.
  • Scholz HC, Hubalek Z, Sedlacek I, et al (2008): Brucella microti sp. nov., isolated from the commonvole Microtus arvalis. Int J Syst Evol Microbiol, 58, 375–382.
  • Shaw WB (1976): Brucella abortus infection in sheep. I. Field case. Br Vet J, 132, 18-26.
  • Stinebring WR, Kunkel JR (1982): In vitro susceptibility of selected isolates of Brucella abortus to penicilin. Am J Vet Res, 43, 545-547.
  • Şahin M, Genç O, Ünver A, et al (2008): Investigation of bovine brucellosis in the Northeastern Turkey. Trop Anim Health Product, 40, 281-286.
There are 26 citations in total.

Details

Primary Language English
Subjects Veterinary Surgery
Journal Section Research Article
Authors

Sevil Erdenlig Gürbilek 0000-0002-0377-2650

Emin Ayhan Baklan 0000-0001-5411-6234

Gülnur Sağlam 0000-0001-7162-2577

Mustafa Sencer Karagül 0000-0001-7215-5229

Ahmet Murat Saytekin 0000-0001-7486-8054

Publication Date June 30, 2022
Published in Issue Year 2022Volume: 69 Issue: 3

Cite

APA Erdenlig Gürbilek, S., Baklan, E. A., Sağlam, G., Karagül, M. S., et al. (2022). Conventional and molecular identification of Brucella isolates from livestock in Turkey. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 69(3), 297-302. https://doi.org/10.33988/auvfd.796785
AMA Erdenlig Gürbilek S, Baklan EA, Sağlam G, Karagül MS, Saytekin AM. Conventional and molecular identification of Brucella isolates from livestock in Turkey. Ankara Univ Vet Fak Derg. June 2022;69(3):297-302. doi:10.33988/auvfd.796785
Chicago Erdenlig Gürbilek, Sevil, Emin Ayhan Baklan, Gülnur Sağlam, Mustafa Sencer Karagül, and Ahmet Murat Saytekin. “Conventional and Molecular Identification of Brucella Isolates from Livestock in Turkey”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 69, no. 3 (June 2022): 297-302. https://doi.org/10.33988/auvfd.796785.
EndNote Erdenlig Gürbilek S, Baklan EA, Sağlam G, Karagül MS, Saytekin AM (June 1, 2022) Conventional and molecular identification of Brucella isolates from livestock in Turkey. Ankara Üniversitesi Veteriner Fakültesi Dergisi 69 3 297–302.
IEEE S. Erdenlig Gürbilek, E. A. Baklan, G. Sağlam, M. S. Karagül, and A. M. Saytekin, “Conventional and molecular identification of Brucella isolates from livestock in Turkey”, Ankara Univ Vet Fak Derg, vol. 69, no. 3, pp. 297–302, 2022, doi: 10.33988/auvfd.796785.
ISNAD Erdenlig Gürbilek, Sevil et al. “Conventional and Molecular Identification of Brucella Isolates from Livestock in Turkey”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 69/3 (June 2022), 297-302. https://doi.org/10.33988/auvfd.796785.
JAMA Erdenlig Gürbilek S, Baklan EA, Sağlam G, Karagül MS, Saytekin AM. Conventional and molecular identification of Brucella isolates from livestock in Turkey. Ankara Univ Vet Fak Derg. 2022;69:297–302.
MLA Erdenlig Gürbilek, Sevil et al. “Conventional and Molecular Identification of Brucella Isolates from Livestock in Turkey”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 69, no. 3, 2022, pp. 297-02, doi:10.33988/auvfd.796785.
Vancouver Erdenlig Gürbilek S, Baklan EA, Sağlam G, Karagül MS, Saytekin AM. Conventional and molecular identification of Brucella isolates from livestock in Turkey. Ankara Univ Vet Fak Derg. 2022;69(3):297-302.