Investigation of deformed wing virus, black queen cell virus, and acute bee paralysis virus infections in honey bees using reverse transcriptase-polymerase chain reaction (RT-PCR) method

Ayşegül Usta; Yakup Yıldırım

doi:10.33988/auvfd.824882

Research Article

Investigation of deformed wing virus, black queen cell virus, and acute bee paralysis virus infections in honey bees using reverse transcriptase-polymerase chain reaction (RT-PCR) method

Year 2022, Volume: 69 Issue: 3, 303 - 311, 30.06.2022

Ayşegül Usta , Yakup Yıldırım

https://doi.org/10.33988/auvfd.824882

Cited By: 11

Abstract

Viruses are one of the most potential risk factors that negatively affect the different life stages of honey bees. This study was conducted to determine the presence of infections caused by the deformed wing virus (DWV), black queen cell virus (BQCV), and acute bee paralysis virus (ABPV) in honey bees in the beekeeping regions of Burdur, along with obtaining information about their prevalence in this particular region. In our study, the adult bees were taken from 31 different beehives and comb samples that had different honey bee breeds and were sampled randomly from 15 beekeeping areas within the region. The collected samples were analyzed using the reverse transcriptase-polymerase chain reaction (RT-PCR), and the prevalence of DWV, BQCV, and ABPV infections were determined to be 74.19% (23/31), 25.81%, (8/31) and 74.19% (23/31), respectively. In this study, the distribution of positive samples and the rates of multiple infections were determined in the colonies. Of the positive honey bee samples, 12 (%38.71) were detected only for one virus, 9 (%29.03) were positive for two viruses (DWV-ABPV) and 8 (%25.81) were positive for all three viruses. In the present study, the presence of the three bee viruses that caused significant damage to the colonies by multiple infections in the Burdur region was determined with RT-PCR. To our knowledge, this is the first report of three mentioned bee infections in honey bees in the Burdur region. The revealed epidemiological conditions lead to the conclusion that serious measures are needed to control these infections in this region.

Keywords

Acute bee paralysis virus, black queen cell virus, deformed wing virus, honey bee, RT-PCR

Supporting Institution

This research was supported by Burdur Mehmet Akif Ersoy University Scientific Research Projects Unit with project number 0586-YL-19.

Project Number

0586-YL-19

Thanks

We thank Dr. A. Anıl Çağırgan and İzmir/Bornova Veterinary Control Institute for their contributions. This study represents part of the thesis submitted by A. Usta to the Virology Department of Veterinary Medicine Faculty of Burdur Mehmet Akif University, Burdur, Turkey, to fulfill the requirements for a master's degree in veterinary medicine.

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