Research Article
Year 2022,
Volume: 69 Issue: 3, 319 - 328, 30.06.2022
Abstract
This study aimed to investigate the effect of creams containing pine and chestnut honey on wound healing in rats. The animals were divided into the following four groups: a control group (A), a group treated with only basic cream ingredients (B), a group treated with pine honey cream (C), and a group treated with chestnut honey cream (D). Full-thickness skin wounds were created on the back of each rat (14 per group) with a 10-mm diameter punch instrument. In each group, skin samples were taken from seven rats on day 7 and from the remaining seven rats on day 14. The effects of the creams on wound healing were assessed by histopathological examination and hydroxyproline assays. The histopathological examination showed that chronic inflammation significantly decreased in group D compared to the control group (P<0.05). Furthermore, the levels of granulation tissue and granulation tissue/fibroblast maturation in skin samples taken on day 14 were also significantly lower in group D compared to the control group (P<0.05). It was observed that the neovascularization values of skin samples taken on day 7 were lower in group D than in the control group (P<0.05). Also, the difference in hydroxyproline levels between groups B and D was statistically significant (P<0.05). The results showed that the cream containing chestnut honey had a positive effect on wound healing.
Keywords
Thanks
This manuscript is derived from the Ph.D. thesis of the first author.
References
- Akershoek JJ, Brouwer KM, Vlig M, et al (2017): Differential effects of Losartan and Atorvastatin in partial and full thickness burn wounds. PLoS One, 12, e0179350.
- Al-Manary M, Al-Meeri A, Al-Habori M (2002): Antioxidant activities and total phenolics of different types of honey. Nutrition Research, 22, 1041-1047.
- Al-Waili NS (2004): Investigating the antimicrobial activity of natural honey and its effects on the pathogenic bacterial infections of surgical wounds and conjunctiva. J Med Food, 7, 210-222.
- Azeredo LC, Azeredo MAA, Souza SR, et al (2003): Protein contents and physiochemical properties in honey samples of Apis mellifera of different floral origins. Food Chem, 80, 249–254.
- Bergman A, Yanai J, Weiss J, et al (1983): Acceleration of wound healing by topical application of honey: An animal model. Am J Surg, 145, 374-376.
- Bogdanov S, Haldimann M, Luginbühl W, et al (2007): Minerals in honey: environmental, geographical and botanical aspects. J Apicult Res, 46, 269–275.
- Castro-Vázquez L, Díaz-Maroto MC, De Torres C, et al (2010): Effect of geographical origin on the chemical and sensory characteristics of chestnut honeys. Food Res Int, 43, 2335-2340.
- Cooper R, Molan P, Harding KG (1999): Antibacterial activity of honey against strains of Staphylococcus aureus from infected wounds. J R Soc Med, 92, 283–285.
- Czubryt MP (2012): Common threads in cardiac fibrosis, infarct scar formation, and wound healing. Fibrogenesis & Tissue Repair, 5, 1-11.
- Delavary BM, Veer WM, Egmond M, et al (2011): Macrophages in skin injury and repair. Immunobiology, 216, 753–762.
- Efem SEE, Iwara CI (1992): The antimicrobial spectrum of honey and its clinical significance. Infection, 20, 227-229.
- Enoch S, Leaper DJ (2005): Basic science of wound healing. Surgery, 23, 37–42.
- Ferreres F, Garcia-Viguera C, Tomas-Lorente F, et al (1993): Hesperetin: a marker of the floral origin of citrus honey. J Sci Food Agric, 61, 121–123.
- Ghaderi R, Afshar M (2004): Topical application of honey for treatment of skin wound in mice. Int J Mol Sci, 29, 185–188.
- Gheldof N, Engeseth NJ (2002): Antioxidant capacity of honeys from various floral sources based on the determination of oxygen radical absorbance capacity and inhibition of in vitro lipoprotein oxidation in human serum samples. J Agric Food Chem, 50, 3050–3055.
- Ghosh PK, Gaba A (2013): Phyto-extracts in wound healing. J Pharm Sci, 16, 760-820.
- Grimble GF (1994): Nutritional antioxidants and the modulation of inflammation: theory and practice. New Horiz, 2, 175–185.
- Haryanto TU, Mukai K, Suriadi JS, et al (2012): Effectiveness of Indonesian honey on the acceleration of cutaneous wound healing: an experimental study in mice. Wounds, 24, 110-119.
- Iftikhar F, Arshad M, Rasheed F, et al (2010): Effects of acacia honey on wound healing in various rat models. Phytother Res, 24, 583-586.
- Kaufman T, Eichenlaub EH, Angel MF, et al (1985): Topical acidification promotes healing of experimental deep partial thickness skin burns: a randomised doubleblind preliminary study. Burns, 12, 84–90.
- Kolayli S, Aliyazıcioglu R, Ulusoy E, et al (2008): Antioxidant and Antimicrobial Activities of Selected Turkish Honeys. Hacettepe J Biol & Chem, 36, 163-172.
- Kumar R, Katoch SS, Sharma S (2006): β-Adrenoceptor agonist treatment reverses denervation atrophy with augmentation of collagen proliferation in denervated mice gastrocnemius muscle. Indian J Exp Biol, 44, 371-376.
- Kwakman PHS, Zaat SAJ (2012): Antibacterial components of honey. IUBMB Life, 64, 48-55.
- Mcloone P, Warnock M, Fyfe L (2016): Honey: A realistic antimicrobial for disorders of the skin. J Microbiol Immunol Infect, 49, 161-167.
- Mercan N, Guvensen A, Celik A, et al (2007): Antimicrobial activity and pollen composition of honey samples collected from different provinces in Turkey. Nat Prod Res, 21, 187-195.
- Mohd ZR, Abu Bakar Zakaria Z, Yusof N, et al (2012): Gelam (Melaleuca spp.) honey-based hydrogel as burn wound dressing. J Evid Based Complementary Altern Med, 1-7.
- Molan PC (1999): The role of honey in the management of wounds. J Wound Care, 8, 415–418.
- Molan PC (2001): Potential of Honey in the Treatment of Wounds and Burns. Am J Clin Dermatol, 2, 13-19.
- Molan PC (2002): Re-introducing honey in the management of wounds and ulcers—theory and practice. Ostomy Wound Manag, 48, 28–40.
- Mukai K, Koike M, Nakamura S, et al (2015): Evaluation of the effects of a combination of japanese honey and hydrocolloid dressing on cutaneous wound healing in male mice. J Evid Based Complementary Altern Med, 2, 1-9.
- Mutlu C, Erbaş M, Tontul SA (2017): Bal ve diğer arı ürünlerinin bazı özellikleri ve insan sağlığı üzerine etkileri. Akademik Gıda, 15, 75-83.
- Nisbet HO, Nisbet C, Yarim M, et al (2010): Effects of Three Types of Honey on Cutaneous Wound Healing. Wounds, 22, 275-283.
- Oryan A, Zaker SR (1998): Effects of topical application of honey on cutaneous wound healing in rabbits. J Vet Med Series A, 45, 181-188.
- Park SI, Sunwoo YY, Jung YJ, et al (2012): Therapeutic effects of acupuncture through enhancement of functional angiogenesis and granulogenesis in rat wound healing. Evid Based Complement Alternat Med, Volume 2012.
- Paydar S, Akrami M, Dehghanian A, et al (2016): A Comparison of the Effects of Alpha and Medical-Grade Honey Ointments on Cutaneous Wound Healing in Rats. Int J Pharm, Volume 2016.
- Rozaini MZ, Zuki ABZ, Noordin M, et al (2004): The effects of different types of honey on tensile strength evaluation of burn wound tissue healing. Int J Appl Res Vet Med, 2, 290–296.
- Sami K. Saikaly SK, Khachemoune A (2017): Honey and Wound Healing: An Update. Am J Clin Dermatol, 18, 237-251.
- Sazegar G, Reza S, Hosseini A, et al (2011): The Effects of Supplemental Zinc and Honey on Wound Healing in Rats. Iran J Basic Med Sci, 4, 391-398.
- Schmidt RJ, Chung LY, Andrews AM (1993): Hydrogen peroxide is a murine (L929) fibroblast cell proliferant at micro- to nanomolar concentrations. 20–23. In: Harding KG, Cherry G, Dealey C, Turner TD (eds). Proceedings of the Second European Conference on Advances in Wound Management; London, UK.
- Sell SA, Wolfe PS, Spence AJ, et al (2012): A preliminary study on the potential of manuka honey and platelet-rich plasma in wound healing. Int J Biomater, 1-14.
- Silver A (1982): Basic physiology of wound healing in the horse. Equine Vet J, 14, 7-15.
- Subrahmanyam M (1998): A prospective randomized clinical and histological study of superficial burn wound healing with honey and silver sulfadiazine. Burns, 24, 157–161.
- Sumitra M, Manikandam P, Gayathri VS, et al (2009): Influence of honey on energy metabolism during wound healing in rats. Sch Res Exch, 1–6.
- Taormina PJ, Niemira BA, Beuchat LR (2001): Inhibitory activity of honey against foodborne pathogens as influenced by the presence of hydrogen peroxide and level of antioxidant power. Int J Food Microbiol, 69, 217-225.
- Tonks A, Cooper RA, Price AJ, et al (2001): Stimulation of TNF-alpha release in monocytes by honey. Cytokine, 14, 240–242.
- Tutun H, Kahraman HA, Aluc Y, et al (2019): Investigation of some metals in honey samples from West Mediterranean region of Turkey. Vet Res Forum, 10, 181.
- Ulusoy E (2012): Bal ve apiterapi. U Bee J, 12, 89-97.
- Yeşil A, Gürkan B, Saraçoğlu Ö, et al (2005): Effect of the pest Marchalina hellenica Gennadius (Homoptera, Margarodidae) on the growth parameters of Pinus brutia Ten. in Muğla region (Turkey). Pol J Ecol, 53, 451-458.
Year 2022,
Volume: 69 Issue: 3, 319 - 328, 30.06.2022
Abstract
Bu çalışmada, ülkemize özgü çam balı ve kestane balı içeren krem formülasyonlarınin yaralar üzerine iyileştirici etkilerinin inclenmesi amaçlanmıştır. Çalışmada, herhangi bir uygulama yapılmayan kontrol grubu (A), sadece krem taşıt maddesi uygulanan grup (B), Çam Balı kremi uygulanan grup (C) ve Kestane balı kremi uygulanan grup (D) olmak üzere 4 farklı denek grubu oluşturuldu. Her grupta (n=14) yer alan sıçanların sırt kısmına 10 mm çapında punch aparatı ile tam kat yara oluşturuldu. Farklı gruplarda yer alan sıçanların yarısının deri örnekleri yara oluşumunu takip eden ilk 7. günde alınırken geri kalan yarısının deri örnekleri 14. günde alındı. Alınan bu deri örneklerine histopatoloji incelemesi ve hidroksiprolin analizi yapıldı. Histopatolojik değerlendirme sonucuna göre, kronik yangının, kestane balı kremi uygulanan grupta kontrol grubuna göre önemli derecede azaldığı tespit edildi (P<0.05). Buna ek olarak, granülasyon doku ve granülasyon dokusu/fibroblast olgunlaşmasının da kestane balı kremi uygulanan ve 14. gün alınan deri örneklerinde, kontrol grubuna göre önemli derecede azaldığı belirlendi (P<0.05). Kestane balı kremi uygulanan grubun 7. günde alınan deri örnekleri neovaskülarizasyon değerlerinin, kontrol grubu neovaskülarizasyon değerlerine göre daha düşük değerlerde olduğu gözlendi (P<0.05). Ayrıca, hidroksiprolin düzeyleri çalışma grupları yönüyle değerlendirildiğinde B ve D grupları arasındaki farklılık istatistiksel açıdan önemli bulundu (P<0.05). Sonuçlar bütün halinde ele alındığında, kestane balı içeren kremin yara iyileşmesi üzerine katkısının olumlu yönde olduğu belirtilmiştir.
Keywords
References
- Akershoek JJ, Brouwer KM, Vlig M, et al (2017): Differential effects of Losartan and Atorvastatin in partial and full thickness burn wounds. PLoS One, 12, e0179350.
- Al-Manary M, Al-Meeri A, Al-Habori M (2002): Antioxidant activities and total phenolics of different types of honey. Nutrition Research, 22, 1041-1047.
- Al-Waili NS (2004): Investigating the antimicrobial activity of natural honey and its effects on the pathogenic bacterial infections of surgical wounds and conjunctiva. J Med Food, 7, 210-222.
- Azeredo LC, Azeredo MAA, Souza SR, et al (2003): Protein contents and physiochemical properties in honey samples of Apis mellifera of different floral origins. Food Chem, 80, 249–254.
- Bergman A, Yanai J, Weiss J, et al (1983): Acceleration of wound healing by topical application of honey: An animal model. Am J Surg, 145, 374-376.
- Bogdanov S, Haldimann M, Luginbühl W, et al (2007): Minerals in honey: environmental, geographical and botanical aspects. J Apicult Res, 46, 269–275.
- Castro-Vázquez L, Díaz-Maroto MC, De Torres C, et al (2010): Effect of geographical origin on the chemical and sensory characteristics of chestnut honeys. Food Res Int, 43, 2335-2340.
- Cooper R, Molan P, Harding KG (1999): Antibacterial activity of honey against strains of Staphylococcus aureus from infected wounds. J R Soc Med, 92, 283–285.
- Czubryt MP (2012): Common threads in cardiac fibrosis, infarct scar formation, and wound healing. Fibrogenesis & Tissue Repair, 5, 1-11.
- Delavary BM, Veer WM, Egmond M, et al (2011): Macrophages in skin injury and repair. Immunobiology, 216, 753–762.
- Efem SEE, Iwara CI (1992): The antimicrobial spectrum of honey and its clinical significance. Infection, 20, 227-229.
- Enoch S, Leaper DJ (2005): Basic science of wound healing. Surgery, 23, 37–42.
- Ferreres F, Garcia-Viguera C, Tomas-Lorente F, et al (1993): Hesperetin: a marker of the floral origin of citrus honey. J Sci Food Agric, 61, 121–123.
- Ghaderi R, Afshar M (2004): Topical application of honey for treatment of skin wound in mice. Int J Mol Sci, 29, 185–188.
- Gheldof N, Engeseth NJ (2002): Antioxidant capacity of honeys from various floral sources based on the determination of oxygen radical absorbance capacity and inhibition of in vitro lipoprotein oxidation in human serum samples. J Agric Food Chem, 50, 3050–3055.
- Ghosh PK, Gaba A (2013): Phyto-extracts in wound healing. J Pharm Sci, 16, 760-820.
- Grimble GF (1994): Nutritional antioxidants and the modulation of inflammation: theory and practice. New Horiz, 2, 175–185.
- Haryanto TU, Mukai K, Suriadi JS, et al (2012): Effectiveness of Indonesian honey on the acceleration of cutaneous wound healing: an experimental study in mice. Wounds, 24, 110-119.
- Iftikhar F, Arshad M, Rasheed F, et al (2010): Effects of acacia honey on wound healing in various rat models. Phytother Res, 24, 583-586.
- Kaufman T, Eichenlaub EH, Angel MF, et al (1985): Topical acidification promotes healing of experimental deep partial thickness skin burns: a randomised doubleblind preliminary study. Burns, 12, 84–90.
- Kolayli S, Aliyazıcioglu R, Ulusoy E, et al (2008): Antioxidant and Antimicrobial Activities of Selected Turkish Honeys. Hacettepe J Biol & Chem, 36, 163-172.
- Kumar R, Katoch SS, Sharma S (2006): β-Adrenoceptor agonist treatment reverses denervation atrophy with augmentation of collagen proliferation in denervated mice gastrocnemius muscle. Indian J Exp Biol, 44, 371-376.
- Kwakman PHS, Zaat SAJ (2012): Antibacterial components of honey. IUBMB Life, 64, 48-55.
- Mcloone P, Warnock M, Fyfe L (2016): Honey: A realistic antimicrobial for disorders of the skin. J Microbiol Immunol Infect, 49, 161-167.
- Mercan N, Guvensen A, Celik A, et al (2007): Antimicrobial activity and pollen composition of honey samples collected from different provinces in Turkey. Nat Prod Res, 21, 187-195.
- Mohd ZR, Abu Bakar Zakaria Z, Yusof N, et al (2012): Gelam (Melaleuca spp.) honey-based hydrogel as burn wound dressing. J Evid Based Complementary Altern Med, 1-7.
- Molan PC (1999): The role of honey in the management of wounds. J Wound Care, 8, 415–418.
- Molan PC (2001): Potential of Honey in the Treatment of Wounds and Burns. Am J Clin Dermatol, 2, 13-19.
- Molan PC (2002): Re-introducing honey in the management of wounds and ulcers—theory and practice. Ostomy Wound Manag, 48, 28–40.
- Mukai K, Koike M, Nakamura S, et al (2015): Evaluation of the effects of a combination of japanese honey and hydrocolloid dressing on cutaneous wound healing in male mice. J Evid Based Complementary Altern Med, 2, 1-9.
- Mutlu C, Erbaş M, Tontul SA (2017): Bal ve diğer arı ürünlerinin bazı özellikleri ve insan sağlığı üzerine etkileri. Akademik Gıda, 15, 75-83.
- Nisbet HO, Nisbet C, Yarim M, et al (2010): Effects of Three Types of Honey on Cutaneous Wound Healing. Wounds, 22, 275-283.
- Oryan A, Zaker SR (1998): Effects of topical application of honey on cutaneous wound healing in rabbits. J Vet Med Series A, 45, 181-188.
- Park SI, Sunwoo YY, Jung YJ, et al (2012): Therapeutic effects of acupuncture through enhancement of functional angiogenesis and granulogenesis in rat wound healing. Evid Based Complement Alternat Med, Volume 2012.
- Paydar S, Akrami M, Dehghanian A, et al (2016): A Comparison of the Effects of Alpha and Medical-Grade Honey Ointments on Cutaneous Wound Healing in Rats. Int J Pharm, Volume 2016.
- Rozaini MZ, Zuki ABZ, Noordin M, et al (2004): The effects of different types of honey on tensile strength evaluation of burn wound tissue healing. Int J Appl Res Vet Med, 2, 290–296.
- Sami K. Saikaly SK, Khachemoune A (2017): Honey and Wound Healing: An Update. Am J Clin Dermatol, 18, 237-251.
- Sazegar G, Reza S, Hosseini A, et al (2011): The Effects of Supplemental Zinc and Honey on Wound Healing in Rats. Iran J Basic Med Sci, 4, 391-398.
- Schmidt RJ, Chung LY, Andrews AM (1993): Hydrogen peroxide is a murine (L929) fibroblast cell proliferant at micro- to nanomolar concentrations. 20–23. In: Harding KG, Cherry G, Dealey C, Turner TD (eds). Proceedings of the Second European Conference on Advances in Wound Management; London, UK.
- Sell SA, Wolfe PS, Spence AJ, et al (2012): A preliminary study on the potential of manuka honey and platelet-rich plasma in wound healing. Int J Biomater, 1-14.
- Silver A (1982): Basic physiology of wound healing in the horse. Equine Vet J, 14, 7-15.
- Subrahmanyam M (1998): A prospective randomized clinical and histological study of superficial burn wound healing with honey and silver sulfadiazine. Burns, 24, 157–161.
- Sumitra M, Manikandam P, Gayathri VS, et al (2009): Influence of honey on energy metabolism during wound healing in rats. Sch Res Exch, 1–6.
- Taormina PJ, Niemira BA, Beuchat LR (2001): Inhibitory activity of honey against foodborne pathogens as influenced by the presence of hydrogen peroxide and level of antioxidant power. Int J Food Microbiol, 69, 217-225.
- Tonks A, Cooper RA, Price AJ, et al (2001): Stimulation of TNF-alpha release in monocytes by honey. Cytokine, 14, 240–242.
- Tutun H, Kahraman HA, Aluc Y, et al (2019): Investigation of some metals in honey samples from West Mediterranean region of Turkey. Vet Res Forum, 10, 181.
- Ulusoy E (2012): Bal ve apiterapi. U Bee J, 12, 89-97.
- Yeşil A, Gürkan B, Saraçoğlu Ö, et al (2005): Effect of the pest Marchalina hellenica Gennadius (Homoptera, Margarodidae) on the growth parameters of Pinus brutia Ten. in Muğla region (Turkey). Pol J Ecol, 53, 451-458.
There are 48 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery |
| Journal Section | Research Article |
| Authors | |
| Publication Date | June 30, 2022 |
| Published in Issue | Year 2022Volume: 69 Issue: 3 |
