Research Article
Effect of probiotic on mast cell density and expression of tryptase, chymase, and TNF-α in the urinary bladder of rats with high cholesterol
Year 2022, Volume: 69 Issue: 3, 273 - 280, 30.06.2022Abstract
In this study, it was aimed to immunohistochemically evaluate the possible effect of probiotic supplementation on rats fed a high cholesterol diet on mast cell heterogeneity and density in the urinary bladder and on the expression of chymase, tryptase and tumor necrosis factor-alpha (TNF-α) cytokine in the urinary bladder. Three groups were formed in the study. For 8 weeks, Group 1 (control group) was fed regular rat diet. Food for Group 2 (feeding +2% cholesterol) (high cholestrol group) was provided for 8 weeks. For the last four weeks, Group 3 (%2 cholesterol + Lactobacillus acidophilus) (probiotic group) was administered L. acidophilus probiotics. Levels of total cholesterol (TC) was measured by using a spectrophotometric autoanalyzer. Serum TC levels were remarkably increased in group 2 compared to the group 1 (P<0.05). TC decreased significantly (P <0.05) in group 3 compared to group 2. The number of mast cells in the control group and the probiotic group were close to each other. Only safranin O (SO) (+) mast cells was increased in the probiotic group. While there was no difference between control and high cholesterol groups, an increase in the number of tryptase mast cells was found in the probiotic group. The number of chymase mast cells declined in the probiotic group compared to control and high cholesterol groups. There was no significant difference in TNF-α immunoreactivity between the three groups. In conclusion, in this study, possible effects of cholesterol and probiotics on mast cell heterogeneity and expression of chymase, tryptase, TNF-α were revealed.
Keywords
References
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- Akyuz AI, Kocaturk UT, Gunel C, et al (2018): Effect of Pycnogenol® on an experimental rat model of allergic conjunctivitis. Graefes Arch Clin Exp Ophthalmol, 256, 1299-1304.
- Atiakshin D, Buchwalow I, Samoilova V, et al (2018): Tryptase as a polyfunctional component of mast cells. Histochem Cell Biol, 149, 461-477.
- Cassard L, Lalanne AI, Garault P, et al (2016): Individual strains of Lactobacillus paracasei differentially inhibit human basophil and mouse mast cell activation. Immun Inflam Dis, 4, 289-299.
- Cheng Y, Zhu Y, Zhang J, et al (2017): Large accumulation of collagen and increased activation of mast cells in hearts of mice with hyperlipidemia. Arq Bras Cardiol, 109, 404-409.
- Çetinel Ş, Çanıllıoğlu YE, Çikler E, et al (2011): Leukotriene D4 receptor antagonist montelukast alleviates protamine sulphate-induced changes in rat urinary bladder. BJU Int, 107, 1320-1325.
- De Zuani M, Dal Secco C, Frossi B (2018): Mast cells at the crossroads of microbiota and IBD. Eur J Immunol, 48, 1929-1937.
- Demirbağ E, Çınar K, Kutlar MH, et al (2012): Distribution and heterogenity of mast cells in small intestine of rat (Rattus rattus). SDÜ Fen Dergisi, 7, 92-99.
- Doron S, Gorbach SL (2006): Probiotics: Their role in the treatment and prevention of disease. Exp Rev Anti Infect Ther, 4, 261-275.
- Dülger EÇ, Çanıllıoğlu YE, Çetinel Ş, et al (2018): Protective Effects of Montelukast Against Stress-Induced Degeneration of the Urinary Bladder. Clin Exp Health Sci, 8, 211-216.
- Enerback L (1966): Mast cells in rat gastrointestinal mucosa: 1. Effects of fixation. Acta Pathol Microbiol Scand, 66, 289-302.
- Ertuğrul T, Çiftci G, Tütüncü Ş (2017): Heterogeneity and distribution of mast cells in renal tissues of rats exposed to formaldehyde. Vet Hekim Der Derg, 88, 26-37.
- Ertugrul T, Tutuncu S, Kabak M, et al (2018): The distribution and heterogeneity of mast cells in tongue from five different avian species. Anat Histol Embryol, 47, 306-312.
- Hayıroğlu AE, Karaca T, Demirtaş S (2016): Streptozotocin-Induced diabetic that histochemical and immunohistochemical examination of mast cells distribution in ovary and uterus during different stages of estrous cycle in rats. Kafkas J Med Sci, 6, 29-37.
- Homayouni A, Bastani P, Ziyadi S, et al (2014): Effects of probiotics on the recurrence of bacterial vaginosis: a review. J Low Genit Tract Dis, 18, 79-86.
- Hu J, La Vecchia C, de Groh M, et al (2012): Dietary cholesterol intake and cancer. Ann Oncol, 23, 491-500.
- Huang ZG, Jin Q, Fan M, et al (2013): Myocardial remodeling in diabetic cardiomyopathy associated with cardiac mast cell activation. PLoS One, 8, e60827.
- Idriss HT, Naismith JH (2000): TNF and the TNF receptor superfamily: structure-function relationship(s). Microsc Res Tech, 50, 184-195.
- Ikonen E (2008): Cellular cholesterol trafficking and compartmentalization. Nat Rev Mol Cell Biol, 9, 125-38.
- Kocatürk T, Ünsal AİA, Günel C, et al (2020): The effect of thymoquınone on experimental model of allergic conjunctivis. KOU Sag Bil Derg, 6, 17-22.
- Kim H, Kwack K, Kim YD, et al (2005): Oral probiotic bacterial administration suppressed allergic responses in an ovalbumin-induced allergy mouse model. FEMS Immunol Med Microbiol, 45, 259-267.
- Li M, Yang K, Wang X, et al (2015): Mast cells infiltration and decreased E-cadherin expression in ketamine-induced cystitis. Toxicol Rep, 2, 205-209.
- Li YJ, Li J, Dai C (2020): The Role of Intestinal Microbiota and mast cell in a rat model of visceral hypersensitivity. J Neurogastroenterol Motil, 6, 529-538.
- Liu C, Ouyang W, Xia J, et al (2018): Tumor Necrosis Factor-α Is Required for Mast Cell-Mediated Host Immunity Against Cutaneous Staphylococcus aureus Infection. J Infect Dis, 218, 64-74.
- Malik ST, Birch BR, Voegeli D, et al (2018). Distribution of mast cell subtypes in interstitial cystitis: implications for novel diagnostic and therapeutic strategies? J Clin Pathol, 71, 840-844.
- Oksaharju A, Kooistra T, Kleemann R, et al (2013): Effects of probiotic Lactobacillus rhamnosus GG and Propionibacterium freudenreichii ssp. Shermanii JS supplementation on intestinal and systemic markers of inflammation in ApoE∗3Leiden mice consuming a high-fat diet. Br J Nutr, 119, 77-85.
- Oksaharju A, Kankainen M, Kekkonen, et al (2011): Probiotic Lactobacillus rhamnosus downregulates FCER1 and HRH4 expression in human mast cells. World J Gastroenterol, 17, 750-759.
- Onody A, Csonka C, Giricz Z, et al (2003): Hyperlipidemia Hinduced by a cholesterol-rich diet leads to enhanced peroxynitrite formation in rat hearts. Cardiovasc Res, 58, 663-670.
- Oottamasathien S, Jia W, Roundy LM, et al (2013): Physiological relevance of LL-37 induced bladder inflammation and mast cells. J Urol, 190, 1596-1602.
- Ozguven AA, Yılmaz O, Taneli F, et al (2014): Protective effect of ketamine against hemorrhagic cystitis in rats receiving ifosfamide. Indian J Pharmacol, 46, 147-151.
- Palm NW, Rosenstein RK, Medzhitov R (2012): Allergic host defences. Nature, 484, 465-472.
- Pejler G (2020): Novel ınsight into the in vivo function of mast cell chymase: lessons from knockouts and ınhibitors. J Innate Immun, 12, 357-372.
- Şenol A, Işler M, Karahan AG, et al (2011): Effect of probiotics on aspirin-induced gastric mucosal lesions. Turk J Gastroenterol, 22, 18-26.
- Traina G (2019): Mast Cells in Gut and Brain and Their Potential Role as an Emerging Therapeutic Target for Neural Diseases. Front Cell Neurosci, 13, 345.
- True LD (1990): Principles of immunohistochemistry. Gower Medical Publishing, New York.
- Tutuncu Ş, Ertugrul T (2019): Immunohistochemical expression of tryptase- chymase and mast cell heterogeneity in capsaicin-treated rat ovaries. IJVAR, 2, 25-31.
- Wang L, Zhou B, Zhou X, et al (2019): Combined lowering effects of rosuvastatin and l. acidophilus on cholesterol levels in rat. J Microbiol Biotechnol, 29, 473-481.
- Yamada T, Murayama T, Mita H, et al (2000): Subtypes of bladder mast cells in interstitial cystitis. Int J Urol, 7, 292-297.
- Yoshida M, Kudoh J, Masunaga K, et al (2010): The effects of chronic hyperlipidemia on bladder function in myocardial infarction-prone Watanabe heritable hyperlipidemic (WHHLMI) rabbits. Neurourol Urodyn, 29, 1350-1354.
- Zhanga X, Huang Q, Wang X, et al (2019): Dietary cholesterol is essential to mast cell activation and associated obesity and diabetes in mice. Biochim Biophys Acta Mol Basis Dis, 1865, 1690-1700.
Year 2022, Volume: 69 Issue: 3, 273 - 280, 30.06.2022
Abstract
References
- Akiyama Y, Maeda D, Morikawa T, et al (2018): Digital quantitative analysis of mast cell infiltration in interstitial cystitis. Neurourol Urodyn, 37, 650-657.
- Akyuz AI, Kocaturk UT, Gunel C, et al (2018): Effect of Pycnogenol® on an experimental rat model of allergic conjunctivitis. Graefes Arch Clin Exp Ophthalmol, 256, 1299-1304.
- Atiakshin D, Buchwalow I, Samoilova V, et al (2018): Tryptase as a polyfunctional component of mast cells. Histochem Cell Biol, 149, 461-477.
- Cassard L, Lalanne AI, Garault P, et al (2016): Individual strains of Lactobacillus paracasei differentially inhibit human basophil and mouse mast cell activation. Immun Inflam Dis, 4, 289-299.
- Cheng Y, Zhu Y, Zhang J, et al (2017): Large accumulation of collagen and increased activation of mast cells in hearts of mice with hyperlipidemia. Arq Bras Cardiol, 109, 404-409.
- Çetinel Ş, Çanıllıoğlu YE, Çikler E, et al (2011): Leukotriene D4 receptor antagonist montelukast alleviates protamine sulphate-induced changes in rat urinary bladder. BJU Int, 107, 1320-1325.
- De Zuani M, Dal Secco C, Frossi B (2018): Mast cells at the crossroads of microbiota and IBD. Eur J Immunol, 48, 1929-1937.
- Demirbağ E, Çınar K, Kutlar MH, et al (2012): Distribution and heterogenity of mast cells in small intestine of rat (Rattus rattus). SDÜ Fen Dergisi, 7, 92-99.
- Doron S, Gorbach SL (2006): Probiotics: Their role in the treatment and prevention of disease. Exp Rev Anti Infect Ther, 4, 261-275.
- Dülger EÇ, Çanıllıoğlu YE, Çetinel Ş, et al (2018): Protective Effects of Montelukast Against Stress-Induced Degeneration of the Urinary Bladder. Clin Exp Health Sci, 8, 211-216.
- Enerback L (1966): Mast cells in rat gastrointestinal mucosa: 1. Effects of fixation. Acta Pathol Microbiol Scand, 66, 289-302.
- Ertuğrul T, Çiftci G, Tütüncü Ş (2017): Heterogeneity and distribution of mast cells in renal tissues of rats exposed to formaldehyde. Vet Hekim Der Derg, 88, 26-37.
- Ertugrul T, Tutuncu S, Kabak M, et al (2018): The distribution and heterogeneity of mast cells in tongue from five different avian species. Anat Histol Embryol, 47, 306-312.
- Hayıroğlu AE, Karaca T, Demirtaş S (2016): Streptozotocin-Induced diabetic that histochemical and immunohistochemical examination of mast cells distribution in ovary and uterus during different stages of estrous cycle in rats. Kafkas J Med Sci, 6, 29-37.
- Homayouni A, Bastani P, Ziyadi S, et al (2014): Effects of probiotics on the recurrence of bacterial vaginosis: a review. J Low Genit Tract Dis, 18, 79-86.
- Hu J, La Vecchia C, de Groh M, et al (2012): Dietary cholesterol intake and cancer. Ann Oncol, 23, 491-500.
- Huang ZG, Jin Q, Fan M, et al (2013): Myocardial remodeling in diabetic cardiomyopathy associated with cardiac mast cell activation. PLoS One, 8, e60827.
- Idriss HT, Naismith JH (2000): TNF and the TNF receptor superfamily: structure-function relationship(s). Microsc Res Tech, 50, 184-195.
- Ikonen E (2008): Cellular cholesterol trafficking and compartmentalization. Nat Rev Mol Cell Biol, 9, 125-38.
- Kocatürk T, Ünsal AİA, Günel C, et al (2020): The effect of thymoquınone on experimental model of allergic conjunctivis. KOU Sag Bil Derg, 6, 17-22.
- Kim H, Kwack K, Kim YD, et al (2005): Oral probiotic bacterial administration suppressed allergic responses in an ovalbumin-induced allergy mouse model. FEMS Immunol Med Microbiol, 45, 259-267.
- Li M, Yang K, Wang X, et al (2015): Mast cells infiltration and decreased E-cadherin expression in ketamine-induced cystitis. Toxicol Rep, 2, 205-209.
- Li YJ, Li J, Dai C (2020): The Role of Intestinal Microbiota and mast cell in a rat model of visceral hypersensitivity. J Neurogastroenterol Motil, 6, 529-538.
- Liu C, Ouyang W, Xia J, et al (2018): Tumor Necrosis Factor-α Is Required for Mast Cell-Mediated Host Immunity Against Cutaneous Staphylococcus aureus Infection. J Infect Dis, 218, 64-74.
- Malik ST, Birch BR, Voegeli D, et al (2018). Distribution of mast cell subtypes in interstitial cystitis: implications for novel diagnostic and therapeutic strategies? J Clin Pathol, 71, 840-844.
- Oksaharju A, Kooistra T, Kleemann R, et al (2013): Effects of probiotic Lactobacillus rhamnosus GG and Propionibacterium freudenreichii ssp. Shermanii JS supplementation on intestinal and systemic markers of inflammation in ApoE∗3Leiden mice consuming a high-fat diet. Br J Nutr, 119, 77-85.
- Oksaharju A, Kankainen M, Kekkonen, et al (2011): Probiotic Lactobacillus rhamnosus downregulates FCER1 and HRH4 expression in human mast cells. World J Gastroenterol, 17, 750-759.
- Onody A, Csonka C, Giricz Z, et al (2003): Hyperlipidemia Hinduced by a cholesterol-rich diet leads to enhanced peroxynitrite formation in rat hearts. Cardiovasc Res, 58, 663-670.
- Oottamasathien S, Jia W, Roundy LM, et al (2013): Physiological relevance of LL-37 induced bladder inflammation and mast cells. J Urol, 190, 1596-1602.
- Ozguven AA, Yılmaz O, Taneli F, et al (2014): Protective effect of ketamine against hemorrhagic cystitis in rats receiving ifosfamide. Indian J Pharmacol, 46, 147-151.
- Palm NW, Rosenstein RK, Medzhitov R (2012): Allergic host defences. Nature, 484, 465-472.
- Pejler G (2020): Novel ınsight into the in vivo function of mast cell chymase: lessons from knockouts and ınhibitors. J Innate Immun, 12, 357-372.
- Şenol A, Işler M, Karahan AG, et al (2011): Effect of probiotics on aspirin-induced gastric mucosal lesions. Turk J Gastroenterol, 22, 18-26.
- Traina G (2019): Mast Cells in Gut and Brain and Their Potential Role as an Emerging Therapeutic Target for Neural Diseases. Front Cell Neurosci, 13, 345.
- True LD (1990): Principles of immunohistochemistry. Gower Medical Publishing, New York.
- Tutuncu Ş, Ertugrul T (2019): Immunohistochemical expression of tryptase- chymase and mast cell heterogeneity in capsaicin-treated rat ovaries. IJVAR, 2, 25-31.
- Wang L, Zhou B, Zhou X, et al (2019): Combined lowering effects of rosuvastatin and l. acidophilus on cholesterol levels in rat. J Microbiol Biotechnol, 29, 473-481.
- Yamada T, Murayama T, Mita H, et al (2000): Subtypes of bladder mast cells in interstitial cystitis. Int J Urol, 7, 292-297.
- Yoshida M, Kudoh J, Masunaga K, et al (2010): The effects of chronic hyperlipidemia on bladder function in myocardial infarction-prone Watanabe heritable hyperlipidemic (WHHLMI) rabbits. Neurourol Urodyn, 29, 1350-1354.
- Zhanga X, Huang Q, Wang X, et al (2019): Dietary cholesterol is essential to mast cell activation and associated obesity and diabetes in mice. Biochim Biophys Acta Mol Basis Dis, 1865, 1690-1700.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary |
| Journal Section | Research Article |
| Authors |
|
| Publication Date | June 30, 2022 |
| Published in Issue | Year 2022Volume: 69 Issue: 3 |
Cite
| Bibtex | @research article { auvfd866431, journal = {Ankara Üniversitesi Veteriner Fakültesi Dergisi}, issn = {1300-0861}, eissn = {1308-2817}, address = {}, publisher = {Ankara University}, year = {2022}, volume = {69}, number = {3}, pages = {273 - 280}, doi = {10.33988/auvfd.866431}, title = {Effect of probiotic on mast cell density and expression of tryptase, chymase, and TNF-α in the urinary bladder of rats with high cholesterol}, key = {cite}, author = {Ertuğrul, Tuğrul and Tütüncü, Şerife and Çiftci, Gülay} } |
| APA | Ertuğrul, T. , Tütüncü, Ş. & Çiftci, G. (2022). Effect of probiotic on mast cell density and expression of tryptase, chymase, and TNF-α in the urinary bladder of rats with high cholesterol . Ankara Üniversitesi Veteriner Fakültesi Dergisi , 69 (3) , 273-280 . DOI: 10.33988/auvfd.866431 |
| MLA | Ertuğrul, T. , Tütüncü, Ş. , Çiftci, G. "Effect of probiotic on mast cell density and expression of tryptase, chymase, and TNF-α in the urinary bladder of rats with high cholesterol" . Ankara Üniversitesi Veteriner Fakültesi Dergisi 69 (2022 ): 273-280 <http://vetjournal.ankara.edu.tr/en/pub/issue/70947/866431> |
| Chicago | Ertuğrul, T. , Tütüncü, Ş. , Çiftci, G. "Effect of probiotic on mast cell density and expression of tryptase, chymase, and TNF-α in the urinary bladder of rats with high cholesterol". Ankara Üniversitesi Veteriner Fakültesi Dergisi 69 (2022 ): 273-280 |
| RIS | TY - JOUR T1 - Effect of probiotic on mast cell density and expression of tryptase, chymase, and TNF-α in the urinary bladder of rats with high cholesterol AU - TuğrulErtuğrul, ŞerifeTütüncü, GülayÇiftci Y1 - 2022 PY - 2022 N1 - doi: 10.33988/auvfd.866431 DO - 10.33988/auvfd.866431 T2 - Ankara Üniversitesi Veteriner Fakültesi Dergisi JF - Journal JO - JOR SP - 273 EP - 280 VL - 69 IS - 3 SN - 1300-0861-1308-2817 M3 - doi: 10.33988/auvfd.866431 UR - https://doi.org/10.33988/auvfd.866431 Y2 - 2021 ER - |
| EndNote | %0 Ankara Üniversitesi Veteriner Fakültesi Dergisi Effect of probiotic on mast cell density and expression of tryptase, chymase, and TNF-α in the urinary bladder of rats with high cholesterol %A Tuğrul Ertuğrul , Şerife Tütüncü , Gülay Çiftci %T Effect of probiotic on mast cell density and expression of tryptase, chymase, and TNF-α in the urinary bladder of rats with high cholesterol %D 2022 %J Ankara Üniversitesi Veteriner Fakültesi Dergisi %P 1300-0861-1308-2817 %V 69 %N 3 %R doi: 10.33988/auvfd.866431 %U 10.33988/auvfd.866431 |
| ISNAD | Ertuğrul, Tuğrul , Tütüncü, Şerife , Çiftci, Gülay . "Effect of probiotic on mast cell density and expression of tryptase, chymase, and TNF-α in the urinary bladder of rats with high cholesterol". Ankara Üniversitesi Veteriner Fakültesi Dergisi 69 / 3 (June 2022): 273-280 . https://doi.org/10.33988/auvfd.866431 |
| AMA | Ertuğrul T. , Tütüncü Ş. , Çiftci G. Effect of probiotic on mast cell density and expression of tryptase, chymase, and TNF-α in the urinary bladder of rats with high cholesterol. Ankara Univ Vet Fak Derg. 2022; 69(3): 273-280. |
| Vancouver | Ertuğrul T. , Tütüncü Ş. , Çiftci G. Effect of probiotic on mast cell density and expression of tryptase, chymase, and TNF-α in the urinary bladder of rats with high cholesterol. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2022; 69(3): 273-280. |
| IEEE | T. Ertuğrul , Ş. Tütüncü and G. Çiftci , "Effect of probiotic on mast cell density and expression of tryptase, chymase, and TNF-α in the urinary bladder of rats with high cholesterol", Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 69, no. 3, pp. 273-280, Jun. 2022, doi:10.33988/auvfd.866431 |
