The aim of this study was to investigate the lipogenic and inflammatory effects of low and high percentage fructose solutions in rats. Wistar albino rats were fed with fructose solutions for 10 weeks. The groups were as follows: Cont (Control), F15 (Fructose 15%), F30 (Fructose 30%), and F60 (Fructose 60%). Rats' body weights were measured weekly. Also, lipogenic and inflammatory gene expression levels, biochemical parameters, and histopathological changes in the liver were investigated. After 10 weeks, it was observed that the animals in the F60 were the heaviest, while the animals in the F30 were the lightest. In all experimental groups, triglycerides were significantly higher than those of controls (P<0.05). In F30 and F60, TNFα, IL-6, and IL-1β were upregulated in the liver compared to control (P<0.05). In addition, SREBP-1c, ChREBP, FAS, ACACA, and SCD-1 were upregulated in all fructose feeding groups compared to Cont (P<0.05). The livers of rats in the F30 and F60 groups had degenerative changes and steatosis. The most detrimental effects of fructose were observed in F60. The concentration of fructose was found to be a very important factor for maintaining normal liver physiology at the molecular level.
This study was financially supported by TUBITAK with 118O381 project number.
Diyetlerdeki karbonhidrat çeşitleri hakkında bilimsel tartışmalar devam etmektedir. Fruktoz, gıda ürünlerinde yaygın olarak kullanılmaktadır. Çalışmada, ratlarda düşük ve yüksek fruktoz solüsyonlarının lipogenik ve inflamatuar etkileri araştırılmıştır. Hayvanlar, 10 hafta süreyle fruktoz solüsyonları ile beslenmiştir. Gruplar: Con (Kontrol), F15 (Fruktoz %15), F30 (Fruktoz %30), F60 (Fruktoz %60) şeklinde olmuştur. F60 en hafif grupken, F30 en ağır grup olarak belirlenmiştir. Trigliserit seviyeleri tüm deneme gruplarında Con'dan önemli ölçüde daha yüksek olmuştur (P<0,05). F30 ve F60'da, TNFα, IL-6 ve IL-1β’nın karaciğerde upregüle olduğu belirlenmiştir (P<0,05). Bununla birlikte tüm fruktoz gruplarında SREBP-1c, ChREBP, FAS, ACACA ve SCD-1 upregüle olmuştur (P<0,05). F30 ve F60 gruplarının karaciğerlerinde dejeneratif değişiklikler ve steatoz belirlenmiştir. Fruktozun en zararlı etkileri F60 grubunda belirlenmiştir. Fruktoz konsantrasyonunun, normal karaciğer fizyolojisini moleküler seviyelerde sürdürmek için çok önemli bir faktör olduğu belirlenmiştir.
Primary Language | English |
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Subjects | Veterinary Surgery |
Journal Section | Research Article |
Authors |
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Project Number | 118O381 |
Publication Date | September 30, 2022 |
Published in Issue | Year 2022Volume: 69 Issue: 4 |
Bibtex | @research article { auvfd855124, journal = {Ankara Üniversitesi Veteriner Fakültesi Dergisi}, issn = {1300-0861}, eissn = {1308-2817}, address = {}, publisher = {Ankara University}, year = {2022}, volume = {69}, number = {4}, pages = {409 - 417}, doi = {10.33988/auvfd.855124}, title = {Molecular, biochemical, and histopathological effects of long-term low and high-percentage fructose consumption on the liver in rats}, key = {cite}, author = {Özkan, Hüseyin and Kutlu, Tuncer and Yakan, Akın and Özsoy, Şule Yurdagül} } |
APA | Özkan, H. , Kutlu, T. , Yakan, A. & Özsoy, Ş. Y. (2022). Molecular, biochemical, and histopathological effects of long-term low and high-percentage fructose consumption on the liver in rats . Ankara Üniversitesi Veteriner Fakültesi Dergisi , 69 (4) , 409-417 . DOI: 10.33988/auvfd.855124 |
MLA | Özkan, H. , Kutlu, T. , Yakan, A. , Özsoy, Ş. Y. "Molecular, biochemical, and histopathological effects of long-term low and high-percentage fructose consumption on the liver in rats" . Ankara Üniversitesi Veteriner Fakültesi Dergisi 69 (2022 ): 409-417 <http://vetjournal.ankara.edu.tr/en/pub/issue/72819/855124> |
Chicago | Özkan, H. , Kutlu, T. , Yakan, A. , Özsoy, Ş. Y. "Molecular, biochemical, and histopathological effects of long-term low and high-percentage fructose consumption on the liver in rats". Ankara Üniversitesi Veteriner Fakültesi Dergisi 69 (2022 ): 409-417 |
RIS | TY - JOUR T1 - Molecular, biochemical, and histopathological effects of long-term low and high-percentage fructose consumption on the liver in rats AU - HüseyinÖzkan, TuncerKutlu, AkınYakan, Şule YurdagülÖzsoy Y1 - 2022 PY - 2022 N1 - doi: 10.33988/auvfd.855124 DO - 10.33988/auvfd.855124 T2 - Ankara Üniversitesi Veteriner Fakültesi Dergisi JF - Journal JO - JOR SP - 409 EP - 417 VL - 69 IS - 4 SN - 1300-0861-1308-2817 M3 - doi: 10.33988/auvfd.855124 UR - https://doi.org/10.33988/auvfd.855124 Y2 - 2021 ER - |
EndNote | %0 Ankara Üniversitesi Veteriner Fakültesi Dergisi Molecular, biochemical, and histopathological effects of long-term low and high-percentage fructose consumption on the liver in rats %A Hüseyin Özkan , Tuncer Kutlu , Akın Yakan , Şule Yurdagül Özsoy %T Molecular, biochemical, and histopathological effects of long-term low and high-percentage fructose consumption on the liver in rats %D 2022 %J Ankara Üniversitesi Veteriner Fakültesi Dergisi %P 1300-0861-1308-2817 %V 69 %N 4 %R doi: 10.33988/auvfd.855124 %U 10.33988/auvfd.855124 |
ISNAD | Özkan, Hüseyin , Kutlu, Tuncer , Yakan, Akın , Özsoy, Şule Yurdagül . "Molecular, biochemical, and histopathological effects of long-term low and high-percentage fructose consumption on the liver in rats". Ankara Üniversitesi Veteriner Fakültesi Dergisi 69 / 4 (September 2022): 409-417 . https://doi.org/10.33988/auvfd.855124 |
AMA | Özkan H. , Kutlu T. , Yakan A. , Özsoy Ş. Y. Molecular, biochemical, and histopathological effects of long-term low and high-percentage fructose consumption on the liver in rats. Ankara Univ Vet Fak Derg. 2022; 69(4): 409-417. |
Vancouver | Özkan H. , Kutlu T. , Yakan A. , Özsoy Ş. Y. Molecular, biochemical, and histopathological effects of long-term low and high-percentage fructose consumption on the liver in rats. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2022; 69(4): 409-417. |
IEEE | H. Özkan , T. Kutlu , A. Yakan and Ş. Y. Özsoy , "Molecular, biochemical, and histopathological effects of long-term low and high-percentage fructose consumption on the liver in rats", Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 69, no. 4, pp. 409-417, Sep. 2022, doi:10.33988/auvfd.855124 |