Research Article
Effect of ascorbic acid on collagen and some inflammatory mediators in rats
Year 2023, Volume: 70 Issue: 3, 303 - 308, 23.06.2023Abstract
Musculoskeletal injuries as a kind of trauma that the human body is exposed to, adversely affect the quality of life and workforce of individuals due to restriction of movement function. This study aimed to evaluate the effects of dose-dependent ascorbic acid (AA) administration on the repair process after gastrocnemius muscle injury in rats. In this study, 5-month-old 66 male Wistar Albino rats were used and rats were randomly divided into 6 groups of 11 each [control, muscle injury, healthy (with 5 mg/10 mg/kg/day AA-treated group), injury (with 5 mg/10 mg/kg/day AA-treated group)]. A linear incision was made in the gastrocnemius muscle of thirty-three animals included in the muscle injury groups. AA (5-10 mg/kg/day) was administered to the four groups intraperitoneally just after surgery once a day. Animals were sacrificed twenty-one days later. Blood and tissue samples were used for cytokine, collagen, and histological measurements. It was found that a dose of 5 mg/kg/day AA administration reduced serum IL-6 and muscle tissue TNF-α levels, and increased liver tissue IL-10 levels. Muscle tissue collagen levels were not statistically different between the groups in parallel with our histological results. In our study, it was demonstrated that vitamin C has effects on inflammatory mediators during muscle tissue repair to explain the mechanism detailed molecular analyzes are needed.
Keywords
ascorbic acid, collagen, inflammation mediators, muscle, wound healing
Supporting Institution
This study was financially supported by Ankara University Scientific Research Projects Coordinator (Project number: 19L0230013).
Thanks
This manuscript is based on master’s thesis of Sibel IŞIN.
References
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- Al Jameil N, Tabassum H, Fatima S, et al (2017): Ameliorating Effect of Vitamin C Against Potassium Dichromate Induced Oxidative Stress and Inflammatory Response in Rats. Int J Pharmacol, 13, 990-999.
- Boncompagni S, Kern H, Rossini K, et al (2007): Structural differentiation of skeletal muscle fibers in the absence of innervation in humans. Proc Natl Acad Sci U S A, 104, 19339-19344.
- Bowie AG, O’Neill LA (2000): Vitamin C inhibits NF-κB activation by TNF via the activation of p38 mitogen-activated protein kinase. J Immunol, 165, 7180-7188.
- Bozonet SM, Carr AC, Pullar JM, et al (2015): Enhanced human neutrophil vitamin C status, chemotaxis and oxidant generation following dietary supplementation with vitamin C-rich SunGold kiwifruit. Nutrients, 7, 2574-2588.
- Butowicz CM, Dearth CL, Hendershot BD (2017): Impact of traumatic lower extremity injuries beyond acute care: Movement-based considerations for resultant longer term secondary health conditions. Adv Wound Care (New Rochelle), 6, 269-278.
- Carr AC, Maggini S (2017): Vitamin C and immune function. Nutrients, 9, 1211.
- Catani MV, Savini I, Rossi A, et al (2005): Biological role of vitamin C in keratinocytes. Nutr Rev, 63, 81-90.
- Ellulu MS, Rahmat A, Patimah I, et al (2015): Effect of vitamin C on inflammation and metabolic markers in hypertensive and/or diabetic obese adults: a randomized controlled trial. Drug Des Devel Ther, 9, 3405-3412.
- Fu SC, Cheng WH, Cheuk YC, et al (2013): Development of vitamin C irrigation saline to promote graft healing in anterior cruciate ligament reconstruction. J Orthop Translat, 1, 67-77.
- Gonzalez ACDO, Costa TF, Andrade ZDA, et al (2016): Wound healing-A literature review. An Bras Dermatol, 91, 614-620.
- Guo SA, Dipietro LA (2010): Factors affecting wound healing. J Dent Res, 89, 219-229.
- Huijskens MJAJ, Walczak M, Koller N, et al (2014): Technical Advance: Ascorbic acid induces development of double-positive T cells from human hematopoietic stem cells in the absence of stromal cells. J Leukoc Biol, 96, 1165-1175.
- Hung LK, Fu SC, Lee YW, et al (2013): Local vitamin-C injection reduced tendon adhesion in a chicken model of flexor digitorum profundus tendon injury. JBJS, 95, e41.
- Kessler B, Rinchai D, Kewcharoenwong C, et al (2017): Interleukin 10 inhibits pro-inflammatory cytokine responses and killing of Burkholderia pseudomallei. Sci Rep, 7, 1-11.
- Kim AY, Lee EM, Lee EJ, et al (2013): Effects of vitamin C on cytotherapy-mediated muscle regeneration. Cell Transplant, 22, 1845-1858.
- Kunduracioglu B, Ulkar B, Sabuncuoglu BT, et al (2006): Effects of hypertonic dextrose on injured rat skeletal muscles. Neurosciences Journal, 11, 93-96.
- Lima CC, Pereira APC, Silva JRF, et al (2009): Ascorbic acid for the healing of skin wounds in rats. Braz J Biol, 69, 1195-1201.
- Loizidis T, Sioga A, Economou L, et al (2007): The role of ascorbic acid and exercise in chronic ischemia of skeletal muscle in rats. J Appl Physiol (1985), 102, 321-330.
- Mackay D, Miller AL (2003): Nutritional support for wound healing. Altern Med Rev, 8, 359-377.
- Nieman DC, Peters EM, Henson DA, et al (2000): Influence of vitamin C supplementation on cytokine changes following an ultramarathon. J Interferon Cytokine Res, 20, 1029-1035.
- Omeroğlu S, Peker T, Türközkan N, et al (2009): High-dose vitamin C supplementation accelerates the Achilles tendon healing in healthy rats. Arch Orthop Trauma Surg, 129, 281-286.
- Su M, Liang X, Xu X, et al (2019): Hepatoprotective benefits of vitamin C against perfluorooctane sulfonate-induced liver damage in mice through suppressing inflammatory reaction and ER stress. Environ Toxicol Pharmacol, 65, 60-65.
- Takisawa S, Funakoshi T, Yatsu T, et al (2019): Vitamin C deficiency causes muscle atrophy and a deterioration in physical performance. Sci Rep, 9, 1-10.
- Tang P, Zhu R, Gu Y, et al (2018): Ascorbic Acid Attenuates Multifidus Muscles Injury and Atrophy after Posterior Lumbar Spine Surgery by Suppressing Inflammation and Oxidative Stress in a Rat Model. Spine, 43, E1249-E1259.
- Tonon E, Ferretti R, Shiratori JH, et al (2012): Ascorbic acid protects the diaphragm muscle against myonecrosis in mdx mice. Nutrition, 28, 686-690.
- Wassall HJH, Devereux G, Seaton A, et al (2013): Complex effects of vitamin E and vitamin C supplementation on in vitro neonatal mononuclear cell responses to allergens. Nutrients, 5, 3337-3351.
- Wilkinson HN, Hardman MJ (2020): Wound healing: cellular mechanisms and pathological outcomes. Open Biol, 10, 200223.
- Woolf AD, Pfleger B (2003): Burden of major musculoskeletal conditions. Bull World Health Organ, 81, 646-656.
- Yun SH, Moon YS, Sohn SH, et al (2012): Effects of cyclic heat stress or vitamin C supplementation during cyclic heat stress on HSP70, inflammatory cytokines, and the antioxidant defense system in Sprague Dawley rats. Exp Anim, 61, 543-553.
Year 2023, Volume: 70 Issue: 3, 303 - 308, 23.06.2023
Abstract
References
- Aguiló A, Monjo M, Moreno C, et al (2014): Vitamin C supplementation does not influence plasma and blood mononuclear cell IL-6 and IL-10 levels after exercise. J Sports Sci, 32, 1659-1669.
- Agyare C, Osafo N, Boakye YD (2018): Biomarkers of Wound Healing. 23-31. In: KH Dogan (Ed), Wound Healing-Current Perspectives. IntechOpen, London.
- Al Jameil N, Tabassum H, Fatima S, et al (2017): Ameliorating Effect of Vitamin C Against Potassium Dichromate Induced Oxidative Stress and Inflammatory Response in Rats. Int J Pharmacol, 13, 990-999.
- Boncompagni S, Kern H, Rossini K, et al (2007): Structural differentiation of skeletal muscle fibers in the absence of innervation in humans. Proc Natl Acad Sci U S A, 104, 19339-19344.
- Bowie AG, O’Neill LA (2000): Vitamin C inhibits NF-κB activation by TNF via the activation of p38 mitogen-activated protein kinase. J Immunol, 165, 7180-7188.
- Bozonet SM, Carr AC, Pullar JM, et al (2015): Enhanced human neutrophil vitamin C status, chemotaxis and oxidant generation following dietary supplementation with vitamin C-rich SunGold kiwifruit. Nutrients, 7, 2574-2588.
- Butowicz CM, Dearth CL, Hendershot BD (2017): Impact of traumatic lower extremity injuries beyond acute care: Movement-based considerations for resultant longer term secondary health conditions. Adv Wound Care (New Rochelle), 6, 269-278.
- Carr AC, Maggini S (2017): Vitamin C and immune function. Nutrients, 9, 1211.
- Catani MV, Savini I, Rossi A, et al (2005): Biological role of vitamin C in keratinocytes. Nutr Rev, 63, 81-90.
- Ellulu MS, Rahmat A, Patimah I, et al (2015): Effect of vitamin C on inflammation and metabolic markers in hypertensive and/or diabetic obese adults: a randomized controlled trial. Drug Des Devel Ther, 9, 3405-3412.
- Fu SC, Cheng WH, Cheuk YC, et al (2013): Development of vitamin C irrigation saline to promote graft healing in anterior cruciate ligament reconstruction. J Orthop Translat, 1, 67-77.
- Gonzalez ACDO, Costa TF, Andrade ZDA, et al (2016): Wound healing-A literature review. An Bras Dermatol, 91, 614-620.
- Guo SA, Dipietro LA (2010): Factors affecting wound healing. J Dent Res, 89, 219-229.
- Huijskens MJAJ, Walczak M, Koller N, et al (2014): Technical Advance: Ascorbic acid induces development of double-positive T cells from human hematopoietic stem cells in the absence of stromal cells. J Leukoc Biol, 96, 1165-1175.
- Hung LK, Fu SC, Lee YW, et al (2013): Local vitamin-C injection reduced tendon adhesion in a chicken model of flexor digitorum profundus tendon injury. JBJS, 95, e41.
- Kessler B, Rinchai D, Kewcharoenwong C, et al (2017): Interleukin 10 inhibits pro-inflammatory cytokine responses and killing of Burkholderia pseudomallei. Sci Rep, 7, 1-11.
- Kim AY, Lee EM, Lee EJ, et al (2013): Effects of vitamin C on cytotherapy-mediated muscle regeneration. Cell Transplant, 22, 1845-1858.
- Kunduracioglu B, Ulkar B, Sabuncuoglu BT, et al (2006): Effects of hypertonic dextrose on injured rat skeletal muscles. Neurosciences Journal, 11, 93-96.
- Lima CC, Pereira APC, Silva JRF, et al (2009): Ascorbic acid for the healing of skin wounds in rats. Braz J Biol, 69, 1195-1201.
- Loizidis T, Sioga A, Economou L, et al (2007): The role of ascorbic acid and exercise in chronic ischemia of skeletal muscle in rats. J Appl Physiol (1985), 102, 321-330.
- Mackay D, Miller AL (2003): Nutritional support for wound healing. Altern Med Rev, 8, 359-377.
- Nieman DC, Peters EM, Henson DA, et al (2000): Influence of vitamin C supplementation on cytokine changes following an ultramarathon. J Interferon Cytokine Res, 20, 1029-1035.
- Omeroğlu S, Peker T, Türközkan N, et al (2009): High-dose vitamin C supplementation accelerates the Achilles tendon healing in healthy rats. Arch Orthop Trauma Surg, 129, 281-286.
- Su M, Liang X, Xu X, et al (2019): Hepatoprotective benefits of vitamin C against perfluorooctane sulfonate-induced liver damage in mice through suppressing inflammatory reaction and ER stress. Environ Toxicol Pharmacol, 65, 60-65.
- Takisawa S, Funakoshi T, Yatsu T, et al (2019): Vitamin C deficiency causes muscle atrophy and a deterioration in physical performance. Sci Rep, 9, 1-10.
- Tang P, Zhu R, Gu Y, et al (2018): Ascorbic Acid Attenuates Multifidus Muscles Injury and Atrophy after Posterior Lumbar Spine Surgery by Suppressing Inflammation and Oxidative Stress in a Rat Model. Spine, 43, E1249-E1259.
- Tonon E, Ferretti R, Shiratori JH, et al (2012): Ascorbic acid protects the diaphragm muscle against myonecrosis in mdx mice. Nutrition, 28, 686-690.
- Wassall HJH, Devereux G, Seaton A, et al (2013): Complex effects of vitamin E and vitamin C supplementation on in vitro neonatal mononuclear cell responses to allergens. Nutrients, 5, 3337-3351.
- Wilkinson HN, Hardman MJ (2020): Wound healing: cellular mechanisms and pathological outcomes. Open Biol, 10, 200223.
- Woolf AD, Pfleger B (2003): Burden of major musculoskeletal conditions. Bull World Health Organ, 81, 646-656.
- Yun SH, Moon YS, Sohn SH, et al (2012): Effects of cyclic heat stress or vitamin C supplementation during cyclic heat stress on HSP70, inflammatory cytokines, and the antioxidant defense system in Sprague Dawley rats. Exp Anim, 61, 543-553.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery, Veterinary Medicine |
| Journal Section | Research Article |
| Authors |
|
| Project Number | 19L0230013 |
| Publication Date | June 23, 2023 |
| Published in Issue | Year 2023Volume: 70 Issue: 3 |
Cite
| Bibtex | @research article { auvfd1019201, journal = {Ankara Üniversitesi Veteriner Fakültesi Dergisi}, issn = {1300-0861}, eissn = {1308-2817}, address = {}, publisher = {Ankara University}, year = {2023}, volume = {70}, number = {3}, pages = {303 - 308}, doi = {10.33988/auvfd.1019201}, title = {Effect of ascorbic acid on collagen and some inflammatory mediators in rats}, key = {cite}, author = {Işın, Sibel and Yazıhan, Nuray and Kesikli, Burcu and Biriken, Derya and Akdaş, Sevginur and Topal Celikkan, Ferda and Bakırarar, Batuhan and Arıbal-ayral, Pelin} } |
| APA | Işın, S. , Yazıhan, N. , Kesikli, B. , Biriken, D. , Akdaş, S. , Topal Celikkan, F. , Bakırarar, B. & Arıbal-ayral, P. (2023). Effect of ascorbic acid on collagen and some inflammatory mediators in rats . Ankara Üniversitesi Veteriner Fakültesi Dergisi , 70 (3) , 303-308 . DOI: 10.33988/auvfd.1019201 |
| MLA | Işın, S. , Yazıhan, N. , Kesikli, B. , Biriken, D. , Akdaş, S. , Topal Celikkan, F. , Bakırarar, B. , Arıbal-ayral, P. "Effect of ascorbic acid on collagen and some inflammatory mediators in rats" . Ankara Üniversitesi Veteriner Fakültesi Dergisi 70 (2023 ): 303-308 <http://vetjournal.ankara.edu.tr/en/pub/issue/78151/1019201> |
| Chicago | Işın, S. , Yazıhan, N. , Kesikli, B. , Biriken, D. , Akdaş, S. , Topal Celikkan, F. , Bakırarar, B. , Arıbal-ayral, P. "Effect of ascorbic acid on collagen and some inflammatory mediators in rats". Ankara Üniversitesi Veteriner Fakültesi Dergisi 70 (2023 ): 303-308 |
| RIS | TY - JOUR T1 - Effect of ascorbic acid on collagen and some inflammatory mediators in rats AU - SibelIşın, NurayYazıhan, BurcuKesikli, DeryaBiriken, SevginurAkdaş, FerdaTopal Celikkan, BatuhanBakırarar, PelinArıbal-ayral Y1 - 2023 PY - 2023 N1 - doi: 10.33988/auvfd.1019201 DO - 10.33988/auvfd.1019201 T2 - Ankara Üniversitesi Veteriner Fakültesi Dergisi JF - Journal JO - JOR SP - 303 EP - 308 VL - 70 IS - 3 SN - 1300-0861-1308-2817 M3 - doi: 10.33988/auvfd.1019201 UR - https://doi.org/10.33988/auvfd.1019201 Y2 - 2022 ER - |
| EndNote | %0 Ankara Üniversitesi Veteriner Fakültesi Dergisi Effect of ascorbic acid on collagen and some inflammatory mediators in rats %A Sibel Işın , Nuray Yazıhan , Burcu Kesikli , Derya Biriken , Sevginur Akdaş , Ferda Topal Celikkan , Batuhan Bakırarar , Pelin Arıbal-ayral %T Effect of ascorbic acid on collagen and some inflammatory mediators in rats %D 2023 %J Ankara Üniversitesi Veteriner Fakültesi Dergisi %P 1300-0861-1308-2817 %V 70 %N 3 %R doi: 10.33988/auvfd.1019201 %U 10.33988/auvfd.1019201 |
| ISNAD | Işın, Sibel , Yazıhan, Nuray , Kesikli, Burcu , Biriken, Derya , Akdaş, Sevginur , Topal Celikkan, Ferda , Bakırarar, Batuhan , Arıbal-ayral, Pelin . "Effect of ascorbic acid on collagen and some inflammatory mediators in rats". Ankara Üniversitesi Veteriner Fakültesi Dergisi 70 / 3 (June 2023): 303-308 . https://doi.org/10.33988/auvfd.1019201 |
| AMA | Işın S. , Yazıhan N. , Kesikli B. , Biriken D. , Akdaş S. , Topal Celikkan F. , Bakırarar B. , Arıbal-ayral P. Effect of ascorbic acid on collagen and some inflammatory mediators in rats. Ankara Univ Vet Fak Derg. 2023; 70(3): 303-308. |
| Vancouver | Işın S. , Yazıhan N. , Kesikli B. , Biriken D. , Akdaş S. , Topal Celikkan F. , Bakırarar B. , Arıbal-ayral P. Effect of ascorbic acid on collagen and some inflammatory mediators in rats. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2023; 70(3): 303-308. |
| IEEE | S. Işın , N. Yazıhan , B. Kesikli , D. Biriken , S. Akdaş , F. Topal Celikkan , B. Bakırarar and P. Arıbal-ayral , "Effect of ascorbic acid on collagen and some inflammatory mediators in rats", Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 70, no. 3, pp. 303-308, Jun. 2023, doi:10.33988/auvfd.1019201 |
