Research Article
Türkiye kıyılarından Avrupa deniz levreği Dicentrarchus labrax (L.)’ı enfekte eden Diplectanum aequans (Diplectanidae: Monogenea)’in morfolojik ve moleküler karakterizasyonunun bütünleştirici bir çalışması
Abstract
Monogeneanlar deniz balıklarının yaygın ektoparazitik yassı kurtlarıdır. Diplectanid monogenean Diplectanum aequans (Wegener, 1857) (Diplectanidae) dünya çapında doğal ve kültüre edilen Avrupa deniz levreği Dicentrarchus labrax (Linnaeus, 1758) türünün en önemli ektoparazitlerden biridir. Bu parazit türü Avrupa deniz levreklerinin solungaçlarında ciddi patolojik hasarlara ve enfekte levreklerde ölüme neden olur. Halen Türkiye kıyılarından D. aequans'ın moleküler verileri hakkında bilgi bulunmamaktadır. Bu çalışmada Türkiye'nin Karadeniz (FAO 37.4.2) ve Ege Denizi (FAO 37.1.3) kıyılarındaki doğal ve kültüre edilen D. labrax (L.)'dan diplectanid monogeneanlar toplandı, morfolojik olarak identifiye edildi ve 28S rRNA ile 18S rRNA parçalarının DNA dizilenmesiyle genetik olarak karakterize edildi. Toplanan diplectanidler ayrıntılı morfolojik özelliklerine ve 28S rRNA ile 18S rRNA genlerinin dizi karakterizasyonuna bağlı olarak açık bir şekilde D. aequans olarak teşhis edildi. Diplectanum aequans'ın enfeksiyon oranı ve ortalama yoğunluğu sırasıyla %100 ve 15,1 idi. Bu çalışma Türkiye kıyılarından örneklenen D. labrax'tan izole edilen D. aequans'ın moleküler (28S ve 18S rRNA) kanıtlarına ilişkin ilk bilgileri sunmaktadır. Karadeniz (FAO 37.4.2) ve Ege Denizinden (FAO 37.1.3) izole edilen D. aequans için yeni 18S ve 28S rRNA dizileri genetik olarak karakterize edilmiştir. Diplectanum aequans'ın 28S ve 18S rRNA dizileri Karadeniz ve Akdeniz'deki Diplectanidae türlerinin filogenetik pozisyonlarını çözmek için kullanılabilir.
Project Number
PYO.VET.1904.17.007.
References
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- Blasco-Costa I, Cutmore SC, Miller TL, et al (2016): Molecular approaches to trematode systematics: ‘best practice’ and implications for future study. Syst Parasitol, 93, 295‒306.
- Castresana, J (2000): Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Mol Biol Evol, 17, 540–552.
- Chero JD, Cruces CL, Sáez G, et al (2021): First data on the parasites of the Pacific kingcroaker Menticirrhus elongatus (Perciformes:Sciaenidae): description of a new species of Rhamnocercoides (Dactylogyridea: Diplectanidae). Acta Parasitol, 66, 1246‒1250.
- Chotnipat S, Miller TL, Knuckey RM, et al (2015): Molecular and morphological evidence for the widespread distribution of Laticola paralatesi infecting wild and farmed Lates calcarifer in Australia. Dis Aquat Organ, 113, 195‒205.
- Desdevises Y, Jovelin R, Jousson O, et al (2000): Comparison of ribosomal DNA sequences of Lamellodiscus spp. (Monogenea, Diplectanidae) parasitising Pagellus (Sparidae, Teleostei) in the North Mediterranean Sea: species divergence and coevolutionary interactions. Int J Parasitol, 30, 741‒746.
- Dezfuli BS, Giari L, Simoni E, et al (2007): Gill histopathology of cultured European sea bass, Dicentrarchus labrax (L.), infected with Diplectanum aequans (Wagener 1857) Diesing 1958 (Diplectanidae: Monogenea). Parasitol Res, 100, 707–713.
- Domingues MV, Boeger WA (2008): Phylogeny and revision of Diplectanidae Monticelli, 1903 (Platyhelminthes: Monogenoidea). Zootaxa, 1698, 1–40.
- FAO FishstatJ (2021): FishStatJ-Software for fishery and aquaculture statistical time series. Available at: http://www.fao.org/fishery/statistics/software/fishstatj/en (Accessed June 20, 2021).
- González-Lanza C, Alvarez-Pellitero P, Sitja-Bobadilla A (1991): Diplectanidae (Monogenea) infestations of sea bass, Dicentrarchus labrax (L.), from the Spanish Mediterranean area. Parasitol Res, 77, 307‒314.
- Guindon S, Gascuel O (2003): A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol, 52, 696–704.
- Hassouna N, Michot B, Bachellerie JP (1984): The complete nucleotide sequence of mouse 28S rDNA gene. Implications for the process of size increase of the large subunit rRNA in higher eukaryotes. Nucleic Acids Res, 12, 3568–3583.
- Hillis DM, Bull JJ (1993): An empirical test of bootstrapping as a method for assessing confidence in phylogenetic analysis. Syst Biol, 42, 182−192.
- Justine JL (2005): Species of Pseudorhabdosynochus Yamaguti, 1958 (Monogenea: Diplectanidae) from Epinephelus fasciatus and E. merra (Perciformes: Serranidae) off New Caledonia and other parts of the Indo-Pacific Ocean, with a comparison of measurements of specimens prepared using different methods, and a description of P. caledonicus n. sp. Syst Parasitol, 62, 1–37.
- Justine JL (2009): A redescription of Pseudorhabdosynochus epinepheli (Yamaguti, 1938), the type-species of Pseudorhabdosynochus Yamaguti, 1958 (Monogenea: Diplectanidae), and the description of P. satyui n. sp. from Epinephelus akaara off Japan. Syst Parasitol, 72, 27−55.
- Kearse M, Moir R, Wilson A, et al (2012): Geneious basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics, 28, 1647–1649.
- Kumar S, Stecher G, Li M, et al (2018): MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol, 35, 1547–1549.
- Lambert A, Maillard C (1974): Parasitisme branchial simultanè pardeux especes de Diplectanum Diesing, 1858 (Monogenea, Monopisthocotylea) chez Dicentrarchus labrax (L. 1758). CR Acad Sci, 279, 1345‒1347.
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- Oliver G (1977): Effect pathogène de la fixation de Diplectanum aequans (Wagener, 1857) Diesing, 1858 (Monogenea, Monopisthocotylea, Diplectanidae) sur les branchies de Dicentrarchus labrax (Linnaeus, 1758), (Pisces, Serranidae). ZF Parasitenkunde, 53, 7–11.
- Reiczigel J, Rózsa L (2005): Quantitative Parasitology 3.0. Budapest, Hungary.
- Rózsa L, Reiczigel J, Majoros G (2000): Quantifying parasites in samples of hosts. J Parasitol, 86, 228‒232.
- Saengpheng C, Purivirojkul W (2020): Pseudorhabdosynochus kasetsartensis n. sp. (Monogenea: Diplectanidae) from the cloudy grouper Epinephelus erythrurus (Valenciennes) (Perciformes: Serranidae) in the lower Gulf of Thailand. Syst Parasitol, 97, 99‒106.
- Silan P, Maillard C (1989): Biologie comparée du développement et discrimination des Diplectanidae ectoparasites du Bar (Teleostei). Ann Sci Nat Zool, 10, 31‒45.
- Šimková A, Plaisance L, Matjusová I, et al (2003): Phylogenetic relationships of the Dactylogyridae Bychowsky, 1933 (Monogenea: Dactylogyridea): the need for the systematic revision of the Ancyrocephalinae Bychowsky, 1937. Syst Parasitol, 54, 1‒11.
- Strona G, Stefani F, Galli P (2009): Field preservation of monogenean parasites for molecular and morphological analyses. Parasitol Int, 58, 51‒54.
- Thompson JD, Higgins DG, Gibson TJ (1994): CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res, 22, 4673‒4680.
- Tingbao Y, Kritsky DC, Yuan S, et al (2006): Diplectanids infesting the gills of the barramundi Lates calcarifer (Bloch) (Perciformes: Centropomidae), with the proposal of Laticola n. g. (Monogenoidea: Diplectanidae). Syst Parasitol, 63, 127‒131.
- Verneau O, Renaud F, Catzeflis F (1997): Evolutionary relationships of sibling tapeworm species (Cestoda) parasitizing teleost fishes. Mol Biol Evol, 14, 630‒636.
- Villar-Torres M, Repullés-Albelda A, Montero F, et al (2019): Neither Diplectanum nor specific: a dramatic twist to the taxonomic framework of Diplectanum (Monogenea: Diplectanidae). Int J Parasitol, 49, 365‒374.
- Whittington ID, Chisholm LA (2008): Diseases caused by Monogenea. 683‒816. In: JC Eiras, H Segner, T Wahli, BG Kapoor (Eds), Fish Diseases Volume 2. Science Publishers, Enfield.
- Wu XY, Li AX, Zhu XQ, et al (2005): Description of Pseudorhabdosynochus seabassi sp. n. (Monogenea: Diplectanidae) from Lates calcarifer and revision of the phylogenetic position of Diplectanum grouperi (Monogenea: Diplectanidae) based on rDNA sequence data. Folia Parasitol, 52, 231‒240.
- Yardimci B, Pekmezci GZ (2012): Gill histopathology in cultured sea bass (Dicentrarchus labrax L.) co-infected by Diplectanum aequans (Wagener, 1857) and Lernanthropus kroyeri (van Beneden, 1851). Ankara Univ Vet Fak Derg, 59, 61‒64.
An integrative study of morphological and molecular characterization of Diplectanum aequans (Diplectanidae: Monogenea) infecting European sea bass Dicentrarchus labrax (Linnaeus, 1758) from Turkish coasts
Abstract
The diplectanid monogenean Diplectanum aequans (D. aequans) (Wegener, 1857) is one of the most important ectoparasites in the wild and cultured European sea bass Dicentrarchus labrax (Dic. labrax) (Linnaeus, 1758) worldwide. Presently, there is no information on the molecular data of D. aequans from Turkish coasts. In the present study, diplectanid monogeneans were collected from wild and cultured Dic. labrax in the Black Sea (FAO area 37.4.2) and Aegean Sea (FAO area 37.1.3) coasts of Türkiye, morphologically identified, and genetically characterized by sequencing of 28S rRNA and 18S rRNA fragments. The collected diplectanids were unambiguously identified as D. aequans based on detailed morphological features and sequence characterization of partial 28S rRNA and 18S rRNA genes. The overall prevalence and mean intensity of D. aequans were 100% and 15.1, respectively. This study reports first information of molecular (28S and 18S rRNA) evidence of D. aequans from Dic. labrax in the Turkish coasts. The new 18S and 28S rRNA sequences for D. aequans isolated from the Black Sea and Aegean Sea are genetically characterized. The 28S and 18S rRNA sequences of D. aequans can be used to resolve the phylogenetic positions of species found in the family Diplectanidae from the Black and Mediterranean Sea.
Supporting Institution
This study was supported by the Scientific Research Council of Ondokuz Mayis University, Samsun, Türkiye, under project no PYO.VET.1904.17.007.
Project Number
PYO.VET.1904.17.007.
Thanks
This work is summarized from the master thesis of DVM Coskun Aydin, Ondokuz Mayis University, Türkiye in 2018. The study was supervised and coordinated by DVM, Ph.D., Associate Professor Gokmen Zafer Pekmezci.
References
- Altschul SF, Madden TL, Schaffer AA, et al (1997): Gapped BLASTn and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res, 25, 3389–3402.
- Blasco-Costa I, Cutmore SC, Miller TL, et al (2016): Molecular approaches to trematode systematics: ‘best practice’ and implications for future study. Syst Parasitol, 93, 295‒306.
- Castresana, J (2000): Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Mol Biol Evol, 17, 540–552.
- Chero JD, Cruces CL, Sáez G, et al (2021): First data on the parasites of the Pacific kingcroaker Menticirrhus elongatus (Perciformes:Sciaenidae): description of a new species of Rhamnocercoides (Dactylogyridea: Diplectanidae). Acta Parasitol, 66, 1246‒1250.
- Chotnipat S, Miller TL, Knuckey RM, et al (2015): Molecular and morphological evidence for the widespread distribution of Laticola paralatesi infecting wild and farmed Lates calcarifer in Australia. Dis Aquat Organ, 113, 195‒205.
- Desdevises Y, Jovelin R, Jousson O, et al (2000): Comparison of ribosomal DNA sequences of Lamellodiscus spp. (Monogenea, Diplectanidae) parasitising Pagellus (Sparidae, Teleostei) in the North Mediterranean Sea: species divergence and coevolutionary interactions. Int J Parasitol, 30, 741‒746.
- Dezfuli BS, Giari L, Simoni E, et al (2007): Gill histopathology of cultured European sea bass, Dicentrarchus labrax (L.), infected with Diplectanum aequans (Wagener 1857) Diesing 1958 (Diplectanidae: Monogenea). Parasitol Res, 100, 707–713.
- Domingues MV, Boeger WA (2008): Phylogeny and revision of Diplectanidae Monticelli, 1903 (Platyhelminthes: Monogenoidea). Zootaxa, 1698, 1–40.
- FAO FishstatJ (2021): FishStatJ-Software for fishery and aquaculture statistical time series. Available at: http://www.fao.org/fishery/statistics/software/fishstatj/en (Accessed June 20, 2021).
- González-Lanza C, Alvarez-Pellitero P, Sitja-Bobadilla A (1991): Diplectanidae (Monogenea) infestations of sea bass, Dicentrarchus labrax (L.), from the Spanish Mediterranean area. Parasitol Res, 77, 307‒314.
- Guindon S, Gascuel O (2003): A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol, 52, 696–704.
- Hassouna N, Michot B, Bachellerie JP (1984): The complete nucleotide sequence of mouse 28S rDNA gene. Implications for the process of size increase of the large subunit rRNA in higher eukaryotes. Nucleic Acids Res, 12, 3568–3583.
- Hillis DM, Bull JJ (1993): An empirical test of bootstrapping as a method for assessing confidence in phylogenetic analysis. Syst Biol, 42, 182−192.
- Justine JL (2005): Species of Pseudorhabdosynochus Yamaguti, 1958 (Monogenea: Diplectanidae) from Epinephelus fasciatus and E. merra (Perciformes: Serranidae) off New Caledonia and other parts of the Indo-Pacific Ocean, with a comparison of measurements of specimens prepared using different methods, and a description of P. caledonicus n. sp. Syst Parasitol, 62, 1–37.
- Justine JL (2009): A redescription of Pseudorhabdosynochus epinepheli (Yamaguti, 1938), the type-species of Pseudorhabdosynochus Yamaguti, 1958 (Monogenea: Diplectanidae), and the description of P. satyui n. sp. from Epinephelus akaara off Japan. Syst Parasitol, 72, 27−55.
- Kearse M, Moir R, Wilson A, et al (2012): Geneious basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics, 28, 1647–1649.
- Kumar S, Stecher G, Li M, et al (2018): MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol, 35, 1547–1549.
- Lambert A, Maillard C (1974): Parasitisme branchial simultanè pardeux especes de Diplectanum Diesing, 1858 (Monogenea, Monopisthocotylea) chez Dicentrarchus labrax (L. 1758). CR Acad Sci, 279, 1345‒1347.
- Lefort V, Longueville JE, Gascuel O (2017): SMS: smart model selection in PhyML. Mol Biol Evol, 34, 2422–2424.
- Llorente I, Fernández-Polanco J, Baraibar-Diez E, et al (2020): Assessment of the economic performance of the seabream and seabass aquaculture industry in the European Union. Mar Policy, 117, 103876.
- Oliver G (1968): Recherches sur les Diplectanidae (Monogenea) parasites de téléostéens du Golfe du Lion. I. Diplectaninae Monticelli, 1903. Vie Milieu A Biol Mar, 19, 95‒138.
- Oliver G (1977): Effect pathogène de la fixation de Diplectanum aequans (Wagener, 1857) Diesing, 1858 (Monogenea, Monopisthocotylea, Diplectanidae) sur les branchies de Dicentrarchus labrax (Linnaeus, 1758), (Pisces, Serranidae). ZF Parasitenkunde, 53, 7–11.
- Reiczigel J, Rózsa L (2005): Quantitative Parasitology 3.0. Budapest, Hungary.
- Rózsa L, Reiczigel J, Majoros G (2000): Quantifying parasites in samples of hosts. J Parasitol, 86, 228‒232.
- Saengpheng C, Purivirojkul W (2020): Pseudorhabdosynochus kasetsartensis n. sp. (Monogenea: Diplectanidae) from the cloudy grouper Epinephelus erythrurus (Valenciennes) (Perciformes: Serranidae) in the lower Gulf of Thailand. Syst Parasitol, 97, 99‒106.
- Silan P, Maillard C (1989): Biologie comparée du développement et discrimination des Diplectanidae ectoparasites du Bar (Teleostei). Ann Sci Nat Zool, 10, 31‒45.
- Šimková A, Plaisance L, Matjusová I, et al (2003): Phylogenetic relationships of the Dactylogyridae Bychowsky, 1933 (Monogenea: Dactylogyridea): the need for the systematic revision of the Ancyrocephalinae Bychowsky, 1937. Syst Parasitol, 54, 1‒11.
- Strona G, Stefani F, Galli P (2009): Field preservation of monogenean parasites for molecular and morphological analyses. Parasitol Int, 58, 51‒54.
- Thompson JD, Higgins DG, Gibson TJ (1994): CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res, 22, 4673‒4680.
- Tingbao Y, Kritsky DC, Yuan S, et al (2006): Diplectanids infesting the gills of the barramundi Lates calcarifer (Bloch) (Perciformes: Centropomidae), with the proposal of Laticola n. g. (Monogenoidea: Diplectanidae). Syst Parasitol, 63, 127‒131.
- Verneau O, Renaud F, Catzeflis F (1997): Evolutionary relationships of sibling tapeworm species (Cestoda) parasitizing teleost fishes. Mol Biol Evol, 14, 630‒636.
- Villar-Torres M, Repullés-Albelda A, Montero F, et al (2019): Neither Diplectanum nor specific: a dramatic twist to the taxonomic framework of Diplectanum (Monogenea: Diplectanidae). Int J Parasitol, 49, 365‒374.
- Whittington ID, Chisholm LA (2008): Diseases caused by Monogenea. 683‒816. In: JC Eiras, H Segner, T Wahli, BG Kapoor (Eds), Fish Diseases Volume 2. Science Publishers, Enfield.
- Wu XY, Li AX, Zhu XQ, et al (2005): Description of Pseudorhabdosynochus seabassi sp. n. (Monogenea: Diplectanidae) from Lates calcarifer and revision of the phylogenetic position of Diplectanum grouperi (Monogenea: Diplectanidae) based on rDNA sequence data. Folia Parasitol, 52, 231‒240.
- Yardimci B, Pekmezci GZ (2012): Gill histopathology in cultured sea bass (Dicentrarchus labrax L.) co-infected by Diplectanum aequans (Wagener, 1857) and Lernanthropus kroyeri (van Beneden, 1851). Ankara Univ Vet Fak Derg, 59, 61‒64.
There are 35 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery, Veterinary Sciences (Other) |
| Journal Section | Research Article |
| Authors | |
| Project Number | PYO.VET.1904.17.007. |
| Publication Date | June 23, 2023 |
| Published in Issue | Year 2023Volume: 70 Issue: 3 |
