Research Article
Year 2024,
Volume: 71 Issue: 2, 157 - 163, 01.04.2024
Abstract
References
- Almecija G, Schimmerling M, Del Cont A, et al (2022): Varroa destructor resistance to tau-fluvalinate: relationship between in vitro phenotypic test and VGSC L925V mutation. Pest Manag Sci, 78, 5097-5105.
- Bahreini R, Nasr M, Docherty C, et al (2021): New bioassay cage methodology for in vitro studies on Varroa destructor and Apis mellifera. PLoS One, 16.
- Bruneau E, Jacobs F, Trouiller J (1997): Results of a campaign for the detection of Varroa resistance to pyrethroids in Belgium (In French). Belg Apic, 61, 294–297.
- Colin ME, Vandame R, Jourdan P, et al (1997): Fluvalinate resistance of Varroa jacobsoni Oudemans (Acari: Varroidae) in Mediterranean apiaries of France. Apidologie, 28, 375–384.
- Colin T, Lim MY, Quarrell SR, et al (2019): Effects of thymol on European honey bee hygienic behaviour. Apidologie, 50, 141–152.
- Council Directive 81/852/EEC (2001): repealed by 2001/82/ЕC on the approximation of the laws of the Member States relating to analytical, pharmaco-toxicological and clinical standards and protocols in respect of the testing of veterinary medicinal products.
- Dahlgren L, Johnson RM, Siegfried BD, et al (2012): Comparative toxicity of acaricides to honey bee (Hymenoptera: Apidae) workers and queens. J Econ Entom, 105, 1895-1902.
- Demirel M, Keskin G, Kumral NA (2019): Varroa mücadelesinde sentetik ve organik akarisitlerin kullanım olanakları. Uludag Bee J, 19, 96-109.
- Elzen PJ, Eischen FA, Baxter JR, et al (1999): Detection of resistance in US Varroa jacobsoni Oud. (Mesostigmata: Varroidae) to the acaricide fluvalinate. Apidologie, 30, 13–17.
- Elzen PJ, Westervelt D (2004): A scientific note on reversion of fluvalinate resistance to a degree susceptibility in Varroa destructor, Apidologie, 35, 519–520.
- Etxegarai-Legarreta O, Sanchez-Famoso V (2022): The Role of beekeeping in the generation of goods and services: the interrelation between environmental, socioeconomic, and sociocultural utilities. Agriculture, 12, 551.
- González‐Cabrera J, Bumann H, Rodríguez‐Vargas S, et al (2018): A single mutation is driving resistance to pyrethroids in European populations of the parasitic mite, Varroa destructor. J Pest Sci, 91, 1137–1144.
- Gracia-Salinas MJ, Ferrer-Dufol M, Latorre-Castro E, et al (2006): Detection of fluvalinate resistance in Varroa destructor in Spanish apiaries. J Apic Res, 45, 101-105.
- Guideline on veterinary medicinal products controlling Varroa destructor parasitosis in bees (2008): EMA/CVMP/EWP/459883/2008, Committee for Medicinal Products for Veterinary Use (CVMP), 15 November 2010.
- Gurgulova K, Georgieva T, Tsvetanov T, et al (2011): Comparative testing of veterinary medicinal products against varroоsis. Anim Sci, XLVIII, 86-93.
- Gurgulova K, Zhelyaskova I, Popova V (2004): Metican /Ecostop/ against Varroatosis among bees. Apiacta, 38, 307-316.
- Gurgulova K, Zhelyazkova I, Popova V (2008). Varroa Control in the Republic of Bulgaria, 310-316. In: Book of Abstract and Paper of 1st International Beekeeping and Pine Honey Congress, Mugla.
- Jack CJ, Ellis JD (2021): Integrated pest management control of Varroa destructor (Acari: Varroidae), the most damaging pest of (Apis mellifera L. (Hymenoptera: Apidae)) colonies. J Insect Sci, 21, 6.
- Khan SU, Anjum SI, Ansari MJ, et al (2019): Antimicrobial potentials of medicinal plant’s extract and their derived silver nanoparticles: A focus on honey bee pathogen. Saudi J Biol Sci, 26, 1815-1834.
- Maggi MD, Ruffinengo SR, Mendoza Y, et al (2011): Susceptibility of Varroa destructor (Acari: Varroidae) to synthetic acaricides in Uruguay: Varroa mites' potential to develop acaricide resistance. Parasitol Res, 108, 815–821.
- Milani N (1995): The resistance of Varroa jacobsoni Oud. to pyretroids: a laboratory assay. Apidologie, 26, 415–429.
- Milani N (1999): The resistance of Varroa jacobsoni Oud. to acaricides. Apidologie, 30, 229–234.
- Milani N, Vedova GD (2002): Decline in the proportion of mites resistant to fluvalinate in a population of Varroa destructor not treated with pyrethroids, Apidologie 33, 417–422.
- OIE Terrestrial Manual (2013): Section 3. 2. 7, 2013.
- Ordinance on the requirements to the data contained in the documentation for issuing a license for use of VMP (2017):- № – 60 / SG. 50 of 25.06.2006; SG. 74 of 12 September 2017 (in Bulgarian).
- Pocol CB, Šedík P, Brumă IS, et al (2021): Organic beekeeping practices in Romania: status and perspectives towards a sustainable development. Agriculture, 11, 281.
- Rinkevich FD, Danka RG, Healy KB (2017): Influence of Varroa Mite (Varroa destructor) management practices on insecticide sensitivity in the honey bee (Apis mellifera). Insects, 8, 9.
- Salkova D (2020): Resistance of Varroa destructor mite to some acaricides), 28-35. In: Proceedings of the 11th Workshop on Experimental Models and Methods in Biomedical Research, ISSN: 1314-9091.
- Salkova D (2020): Toxicity of the most popular acaricides against Varroa destructor mite, 36-42. In: Proceedings of the 11th Workshop on Experimental Models and Methods in Biomedical Research, ISSN: 1314-9091.
- Shumkova R, Neov B, Sirakova D, et al (2018): Molecular detection and phylogenetic assessment of six honeybee viruses in Apis mellifera L. colonies in Bulgaria. Peer J, 6, e5077.
- StatSoft Inc (2014): STATISTICA (data analysis software system), Version 12. Available at: www.statsoft.com.
- Tlak Gajger I, Svečnjak L, Bubalo D, et al (2020): Control of Varroa destructor mite infestations at experimental apiaries situated in Croatia. Diversity, 12, 12.
- Topal E, Cornea-Cipcigan M, İvgin Tunca R, et al (2020): The use of medicinal aromatic plants against bee diseases and pests. Bee Studies, 12, 5-11.
- Vandame R, Colin ME, Belzunces LP, et al (1995): Résistance de Varroa au fluvalinate. Le Carnet Européen, 3, 5–11.
Clinical trial of the efficiency of three different compositions of acaricidal substances against varoosis in honey bee colonies
Abstract
This study aimed to evaluate and compare the varroacidal efficacy and mite mortality dynamic during autumn treatment of honey bee colonies in two experimental areas (Boychinovtsi-Northwestern Bulgaria and Zlatiya-Northeastern Bulgaria), treated with three available veterinary medicinal substances. The clinical studies were conducted on the efficiency of the three acaricidal combinations (AC) - one, based on 3.6 mg flumethrin/strip (AC-1) and two contents of essential oils (first one with composition: 5 g thymol plus 2 g peppermint oil/lamellae (AC-2), and the second one with composition: 4 g thymol plus 2 g peppermint oil/ lamellae (AC-3), in the autumn of 2017. We used the product containing coumaphos and an additive with oxalic acid for the control treatment. Methods for establishing the levels of Varroa destructor infestation in bees and in brood were used according to OIE Terrestrial Manual. After 35 days of AC-1 exposure, 94.5% and 87.82% efficiency were achieved in the apiaries in Boychinovtsi and Zlatiya, respectively. Efficiencies of the combinations tested (AC-2 and AC-3) for 45 days were detected high only in the Zlatiya apiary (97% and 95%), also 91% and 80% in the Boychinovtsi apiary, respectively. The results of the experiments showed the absence of resistance to the tested substances.
References
- Almecija G, Schimmerling M, Del Cont A, et al (2022): Varroa destructor resistance to tau-fluvalinate: relationship between in vitro phenotypic test and VGSC L925V mutation. Pest Manag Sci, 78, 5097-5105.
- Bahreini R, Nasr M, Docherty C, et al (2021): New bioassay cage methodology for in vitro studies on Varroa destructor and Apis mellifera. PLoS One, 16.
- Bruneau E, Jacobs F, Trouiller J (1997): Results of a campaign for the detection of Varroa resistance to pyrethroids in Belgium (In French). Belg Apic, 61, 294–297.
- Colin ME, Vandame R, Jourdan P, et al (1997): Fluvalinate resistance of Varroa jacobsoni Oudemans (Acari: Varroidae) in Mediterranean apiaries of France. Apidologie, 28, 375–384.
- Colin T, Lim MY, Quarrell SR, et al (2019): Effects of thymol on European honey bee hygienic behaviour. Apidologie, 50, 141–152.
- Council Directive 81/852/EEC (2001): repealed by 2001/82/ЕC on the approximation of the laws of the Member States relating to analytical, pharmaco-toxicological and clinical standards and protocols in respect of the testing of veterinary medicinal products.
- Dahlgren L, Johnson RM, Siegfried BD, et al (2012): Comparative toxicity of acaricides to honey bee (Hymenoptera: Apidae) workers and queens. J Econ Entom, 105, 1895-1902.
- Demirel M, Keskin G, Kumral NA (2019): Varroa mücadelesinde sentetik ve organik akarisitlerin kullanım olanakları. Uludag Bee J, 19, 96-109.
- Elzen PJ, Eischen FA, Baxter JR, et al (1999): Detection of resistance in US Varroa jacobsoni Oud. (Mesostigmata: Varroidae) to the acaricide fluvalinate. Apidologie, 30, 13–17.
- Elzen PJ, Westervelt D (2004): A scientific note on reversion of fluvalinate resistance to a degree susceptibility in Varroa destructor, Apidologie, 35, 519–520.
- Etxegarai-Legarreta O, Sanchez-Famoso V (2022): The Role of beekeeping in the generation of goods and services: the interrelation between environmental, socioeconomic, and sociocultural utilities. Agriculture, 12, 551.
- González‐Cabrera J, Bumann H, Rodríguez‐Vargas S, et al (2018): A single mutation is driving resistance to pyrethroids in European populations of the parasitic mite, Varroa destructor. J Pest Sci, 91, 1137–1144.
- Gracia-Salinas MJ, Ferrer-Dufol M, Latorre-Castro E, et al (2006): Detection of fluvalinate resistance in Varroa destructor in Spanish apiaries. J Apic Res, 45, 101-105.
- Guideline on veterinary medicinal products controlling Varroa destructor parasitosis in bees (2008): EMA/CVMP/EWP/459883/2008, Committee for Medicinal Products for Veterinary Use (CVMP), 15 November 2010.
- Gurgulova K, Georgieva T, Tsvetanov T, et al (2011): Comparative testing of veterinary medicinal products against varroоsis. Anim Sci, XLVIII, 86-93.
- Gurgulova K, Zhelyaskova I, Popova V (2004): Metican /Ecostop/ against Varroatosis among bees. Apiacta, 38, 307-316.
- Gurgulova K, Zhelyazkova I, Popova V (2008). Varroa Control in the Republic of Bulgaria, 310-316. In: Book of Abstract and Paper of 1st International Beekeeping and Pine Honey Congress, Mugla.
- Jack CJ, Ellis JD (2021): Integrated pest management control of Varroa destructor (Acari: Varroidae), the most damaging pest of (Apis mellifera L. (Hymenoptera: Apidae)) colonies. J Insect Sci, 21, 6.
- Khan SU, Anjum SI, Ansari MJ, et al (2019): Antimicrobial potentials of medicinal plant’s extract and their derived silver nanoparticles: A focus on honey bee pathogen. Saudi J Biol Sci, 26, 1815-1834.
- Maggi MD, Ruffinengo SR, Mendoza Y, et al (2011): Susceptibility of Varroa destructor (Acari: Varroidae) to synthetic acaricides in Uruguay: Varroa mites' potential to develop acaricide resistance. Parasitol Res, 108, 815–821.
- Milani N (1995): The resistance of Varroa jacobsoni Oud. to pyretroids: a laboratory assay. Apidologie, 26, 415–429.
- Milani N (1999): The resistance of Varroa jacobsoni Oud. to acaricides. Apidologie, 30, 229–234.
- Milani N, Vedova GD (2002): Decline in the proportion of mites resistant to fluvalinate in a population of Varroa destructor not treated with pyrethroids, Apidologie 33, 417–422.
- OIE Terrestrial Manual (2013): Section 3. 2. 7, 2013.
- Ordinance on the requirements to the data contained in the documentation for issuing a license for use of VMP (2017):- № – 60 / SG. 50 of 25.06.2006; SG. 74 of 12 September 2017 (in Bulgarian).
- Pocol CB, Šedík P, Brumă IS, et al (2021): Organic beekeeping practices in Romania: status and perspectives towards a sustainable development. Agriculture, 11, 281.
- Rinkevich FD, Danka RG, Healy KB (2017): Influence of Varroa Mite (Varroa destructor) management practices on insecticide sensitivity in the honey bee (Apis mellifera). Insects, 8, 9.
- Salkova D (2020): Resistance of Varroa destructor mite to some acaricides), 28-35. In: Proceedings of the 11th Workshop on Experimental Models and Methods in Biomedical Research, ISSN: 1314-9091.
- Salkova D (2020): Toxicity of the most popular acaricides against Varroa destructor mite, 36-42. In: Proceedings of the 11th Workshop on Experimental Models and Methods in Biomedical Research, ISSN: 1314-9091.
- Shumkova R, Neov B, Sirakova D, et al (2018): Molecular detection and phylogenetic assessment of six honeybee viruses in Apis mellifera L. colonies in Bulgaria. Peer J, 6, e5077.
- StatSoft Inc (2014): STATISTICA (data analysis software system), Version 12. Available at: www.statsoft.com.
- Tlak Gajger I, Svečnjak L, Bubalo D, et al (2020): Control of Varroa destructor mite infestations at experimental apiaries situated in Croatia. Diversity, 12, 12.
- Topal E, Cornea-Cipcigan M, İvgin Tunca R, et al (2020): The use of medicinal aromatic plants against bee diseases and pests. Bee Studies, 12, 5-11.
- Vandame R, Colin ME, Belzunces LP, et al (1995): Résistance de Varroa au fluvalinate. Le Carnet Européen, 3, 5–11.
There are 34 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Sciences (Other) |
| Journal Section | Research Article |
| Authors | |
| Early Pub Date | September 21, 2023 |
| Publication Date | April 1, 2024 |
| Published in Issue | Year 2024Volume: 71 Issue: 2 |
