Determination of antimicrobial susceptibility patterns of fish and rearing water originated Aeromonas isolates
Öz
Aeromonas spp. are Gram negative bacilli that are widely distributed globally in aquatic environments and cause infections in both human and cold-blooded animals. In this study, we aimed to investigate antimicrobial susceptibility of Aeromonas isolates from fish (Rainbow trout and Mullet) and rearing water in Turkey. A total of 45 Aeromonas isolates (20 A. sobria, 10 A. hydrophila, nine A. salmonicida, four A. bestiarum, two A. veroni) from three different geographic regions (Blacksea, Mediterrian, Aegean) of Turkey were included in the study. Bacterial susceptibilities of the isolates to 13 different antimicrobials were evaluated by disk diffusion method. Among the antimicrobials used in the study, gentamicin was the only antimicrobial agent to which all the isolates were susceptible. Similarly, florfenicol and ciprofloxacin were highly active (resistance rate 8.9%), whereas the lowest susceptibility rates were noted for amoxcillin (17.8%), ampicillin (22.2%), and oxolonic acid (35.6%). In conclusion, detection of different rate of drug resistance in the study, suggested that selection of appropriate antimicrobial agents is important for treatment of both fish and human diseases. Also in the light of the data an effective national antimicrobial resistance monitoring programs are required for Aeromonas spp
Anahtar Kelimeler
Kaynakça
- Akşit A, Kum V (2008): Gökkuşağı Alabalıkları (Oncorhynchus mykiss, Walbaum 1792)’nda sık görülen patojen mikroorganizmaların tespiti ve antibiyotik duyarlılık düzeylerinin belirlenmesi. YYU Vet Fak Derg, 19, 1-7.
- Ansari M, Rahimi E, Raissy M (2011): Antibiotic susceptibility and resistance of Aeromonas spp. isolated from fish. African J Microbiol Res, 5, 5772-5775.
- Aravena-Roman M, Inglis TJJ, Henderson B ve ark. (2012): Antimicrobial susceptibilities of Aeromonas strains isolated from clinical and environmental sources to 26 antimicrobial agents. Antimicrob Agents Chemother, 56, 1110-1112.
- Austin B, Austin DA (2007): Bacterial fish pathogens, Diseases of farmed and wild fish. Springer-Praxis Publishing, Chichester, UK.
- Beaz‐Hidalgo R, Figueras MJ (2013): Aeromonas spp. whole genomes and virulence factors implicated in fish disease. J Fish Dis, 36, 371-388.
- Cizek A, Dolejska M, Sochorova R ve ark. (2010): Antimicrobial resistance and its genetic determinants in aeromonads isolated in ornamental (koi) carp (Cyprinus carpio koi) and common carp (Cyprinus carpio). Vet Microbiol, 142, 435-439.
- Clinical and Laboratory Standards Institute (2012): Performance Susceptibility Tests; Approved, M100- S23, Wayne. for Antimicrobial Disk
- Clinical and Laboratory Standards Institute (2013): Performance Standards for Antimicrobial Susceptibility 23th informational supplement. CLSI Document M100- S23, (ISBN1-56238-865-7-Print:1-56238-866-5–Electronic) Clinical and Laboratory Standards Institute, 950 West Valley Road, Suite 2500, Wayne, Pennsylvania, 19087 USA.
- Daood N (2012): Isolation and antibiotic susceptibility of Aeromonas spp. from freshwater fish farm and farmed carp. Damascus University Journal for Basıc Sciences, 28, 27-39.
- Dias C, Mota V, Martinez-Murcia A ve ark. (2012): Antimicrobial resistance patterns of Aeromonas spp. isolated from Ornamental fish. J Aquacult Res Dev, 3, 131.
- Dinç G, Cengiz S, Söğüt MÜ (2013): Tüketime sunulan alabalıklarda Aeromonas hydrophila varlığı ve antibakteriyel duyarlılıklarının saptanması. Mersin Üniv Sağlık Bilim Derg, 6, 21-25.
- Durmaz Y, Türk N (2009): Alabalık işletmelerinden motil Aeromonasların izolasyonu ve antibiyotiklere duyarlılıklarının saptanması. Kafkas Univ Vet Fak Derg, 15, 357-361.
- Goni-Urriza M, Pineau L, Capdepuy M ve ark. (2000): Antimicrobial resistance of mesophilic Aeromonas spp. isolated from two European rivers. J Antimicrob Chemother, 46, 297-301.
- Guz L, Kozinska A (2004): Antibiotic susceptibility of Aeromonas hydrophila and A. sobria isolated from farmed carp (Cyprinus carpio L). Bull Vet Inst Pulawy, 48, 391- 395.
- Hatha MAA, Vivekanandhan G, Christol JJ (2005): Antibiotic resistance pattern of motile aeromonads from farm raised fresh water fish. Int J Food Microbiol, 98, 131- 134.
- Huddleston JR, Zak JC, Randall MJ (2006): Antimicrobial susceptibilities of Aeromonas spp. isolated from environmental sources. Appl Environment Microbiol, 72, 7036-7042.
- Janda JM, Abbott SL (1998): Evolving concepts regarding the genus Aeromonas: an expanding panorama of species, disease presentations, and unanswered questions. Clin Infect Dis, 27, 332-344.
- Janda JM, Abbott SL (2010): The Genus Aeromonas: Taxonomy, pathogenicity, and infection. Clin Microbiol Rev, 23, 35-73.
- Kaskhedikar M, Chhabra D (2010): Multiple drug resistance in Aeromonas hydrophila isolates of fish. Food Microbiol, 28, 157-168. AR, 20. Laith Najiah M. Antimicrobial susceptibility and histopathology of isolates from diseased catfish, Clarias gariepinus (Burchell). J Aquac Res Development, 5, 2. Aeromonas hydrophila:
- Martin-Carnahan E, Joseph WS (2005): Aeromonas. 556-578. In: DJ Brenner, NR Krieg, JT Staley, GM Garrity (Ed), Bergey´s Manual of Systematic Bacteriology, 2nd Ed., Vol. 2, Springer-Verlag, New York.
- Onuk EE, Çaycı YT, Çoban AY ve ark. (2015): Türkiye’de su kaynaklı Aeromonas izolatlarında saptanan ilk QnrS gen pozitifliği. Mikrobiyol Bul, 49, 114-123.
- Rahman MM, Hossain MN (2013): Antibiotic and herbal sensitivity of some Aeromonas sp. isolates collected from diseased carp fishes. Progress Agricul, 21, 117-129.
- Sreedharan K, Philip R, Singh ISB (2012): Virulence potential and antibiotic susceptibility pattern of motile aeromonads associated with freshwater ornamental fish culture systems: A possible threat to public health. Brazil J Microbiol, 43, 754-765.
- Vila J, Marco F, Soler L ve ark. (2002): In vitro antimicrobial susceptibility of clinical isolates of Aeromonas caviae, Aeromonas hydrophila and Aeromonas veronii biotype sobria. J Antimicrob Chemother, 49, 701- 702.
Balık ve yetiştirme suyu kökenli Aeromonas izolatlarının antimikrobiyal duyarlılıklarının saptanması
Öz
Aeromonas türleri sucul ortamda yaygın olarak bulunan ve hem soğuk kanlı hayvanlarda hem de insanlar da çeşitli hastalıklara neden olabilen Gram negatif basillerdir. Bu çalışma, ülkemizde balık ve yetiştirme sularından izole edilmiş olan Aeromonas izolatlarının antimikrobiyal duyarlılık profillerinin belirlenmesi amacıyla gerçekleştirildi. Çalışmaya üç farklı coğrafik bölgeden (Karadeniz, Akdeniz, Ege) izole edilmiş olan toplam 45 Aeromonas izolatı (20 A. sobria, 10 A. hydrophila, dokuz A. salmonicida, dört A. bestiarum, iki A. veroni) dahil edildi. İzolatların 13 farklı antimikrobiyal ajana karşı antimikrobiyal aktiviteleri disk difüzyon yöntemiyle değerlendirildi. Test edilen antimikrobiyaller içerisinde gentamisin bütün izolatların duyarlı olduğu tek antimikrobiyal olarak belirlendi. Florfenikol ve siprofloksasin’in yüksek düzeyde etkin oldukları (direnç oranı %8.9), en düşük duyarlılık oranın ise amoksisilin (%17.8), ampisilin (%22.2) ve oksolonik asit’e (%35.6) karşı şekillendiği belirlendi. Sonuç olarak, çalışmada farklı oranlarda ilaç direncinin saptanmış olması hem balık hem de insan hastalıklarının tedavisinde uygun antimikrobiyel ajanların seçilmesinin önemini ortaya koymakla birlikte Aeromonas türleri için etkin ulusal antimikrobiyal direnç izleme sistemlerine ihtiyaç duyulduğunu göstermektedir
Anahtar Kelimeler
Aeromonas spp. antimikrobiyal duyarlılık balık yetiştirme suyu
Kaynakça
- Akşit A, Kum V (2008): Gökkuşağı Alabalıkları (Oncorhynchus mykiss, Walbaum 1792)’nda sık görülen patojen mikroorganizmaların tespiti ve antibiyotik duyarlılık düzeylerinin belirlenmesi. YYU Vet Fak Derg, 19, 1-7.
- Ansari M, Rahimi E, Raissy M (2011): Antibiotic susceptibility and resistance of Aeromonas spp. isolated from fish. African J Microbiol Res, 5, 5772-5775.
- Aravena-Roman M, Inglis TJJ, Henderson B ve ark. (2012): Antimicrobial susceptibilities of Aeromonas strains isolated from clinical and environmental sources to 26 antimicrobial agents. Antimicrob Agents Chemother, 56, 1110-1112.
- Austin B, Austin DA (2007): Bacterial fish pathogens, Diseases of farmed and wild fish. Springer-Praxis Publishing, Chichester, UK.
- Beaz‐Hidalgo R, Figueras MJ (2013): Aeromonas spp. whole genomes and virulence factors implicated in fish disease. J Fish Dis, 36, 371-388.
- Cizek A, Dolejska M, Sochorova R ve ark. (2010): Antimicrobial resistance and its genetic determinants in aeromonads isolated in ornamental (koi) carp (Cyprinus carpio koi) and common carp (Cyprinus carpio). Vet Microbiol, 142, 435-439.
- Clinical and Laboratory Standards Institute (2012): Performance Susceptibility Tests; Approved, M100- S23, Wayne. for Antimicrobial Disk
- Clinical and Laboratory Standards Institute (2013): Performance Standards for Antimicrobial Susceptibility 23th informational supplement. CLSI Document M100- S23, (ISBN1-56238-865-7-Print:1-56238-866-5–Electronic) Clinical and Laboratory Standards Institute, 950 West Valley Road, Suite 2500, Wayne, Pennsylvania, 19087 USA.
- Daood N (2012): Isolation and antibiotic susceptibility of Aeromonas spp. from freshwater fish farm and farmed carp. Damascus University Journal for Basıc Sciences, 28, 27-39.
- Dias C, Mota V, Martinez-Murcia A ve ark. (2012): Antimicrobial resistance patterns of Aeromonas spp. isolated from Ornamental fish. J Aquacult Res Dev, 3, 131.
- Dinç G, Cengiz S, Söğüt MÜ (2013): Tüketime sunulan alabalıklarda Aeromonas hydrophila varlığı ve antibakteriyel duyarlılıklarının saptanması. Mersin Üniv Sağlık Bilim Derg, 6, 21-25.
- Durmaz Y, Türk N (2009): Alabalık işletmelerinden motil Aeromonasların izolasyonu ve antibiyotiklere duyarlılıklarının saptanması. Kafkas Univ Vet Fak Derg, 15, 357-361.
- Goni-Urriza M, Pineau L, Capdepuy M ve ark. (2000): Antimicrobial resistance of mesophilic Aeromonas spp. isolated from two European rivers. J Antimicrob Chemother, 46, 297-301.
- Guz L, Kozinska A (2004): Antibiotic susceptibility of Aeromonas hydrophila and A. sobria isolated from farmed carp (Cyprinus carpio L). Bull Vet Inst Pulawy, 48, 391- 395.
- Hatha MAA, Vivekanandhan G, Christol JJ (2005): Antibiotic resistance pattern of motile aeromonads from farm raised fresh water fish. Int J Food Microbiol, 98, 131- 134.
- Huddleston JR, Zak JC, Randall MJ (2006): Antimicrobial susceptibilities of Aeromonas spp. isolated from environmental sources. Appl Environment Microbiol, 72, 7036-7042.
- Janda JM, Abbott SL (1998): Evolving concepts regarding the genus Aeromonas: an expanding panorama of species, disease presentations, and unanswered questions. Clin Infect Dis, 27, 332-344.
- Janda JM, Abbott SL (2010): The Genus Aeromonas: Taxonomy, pathogenicity, and infection. Clin Microbiol Rev, 23, 35-73.
- Kaskhedikar M, Chhabra D (2010): Multiple drug resistance in Aeromonas hydrophila isolates of fish. Food Microbiol, 28, 157-168. AR, 20. Laith Najiah M. Antimicrobial susceptibility and histopathology of isolates from diseased catfish, Clarias gariepinus (Burchell). J Aquac Res Development, 5, 2. Aeromonas hydrophila:
- Martin-Carnahan E, Joseph WS (2005): Aeromonas. 556-578. In: DJ Brenner, NR Krieg, JT Staley, GM Garrity (Ed), Bergey´s Manual of Systematic Bacteriology, 2nd Ed., Vol. 2, Springer-Verlag, New York.
- Onuk EE, Çaycı YT, Çoban AY ve ark. (2015): Türkiye’de su kaynaklı Aeromonas izolatlarında saptanan ilk QnrS gen pozitifliği. Mikrobiyol Bul, 49, 114-123.
- Rahman MM, Hossain MN (2013): Antibiotic and herbal sensitivity of some Aeromonas sp. isolates collected from diseased carp fishes. Progress Agricul, 21, 117-129.
- Sreedharan K, Philip R, Singh ISB (2012): Virulence potential and antibiotic susceptibility pattern of motile aeromonads associated with freshwater ornamental fish culture systems: A possible threat to public health. Brazil J Microbiol, 43, 754-765.
- Vila J, Marco F, Soler L ve ark. (2002): In vitro antimicrobial susceptibility of clinical isolates of Aeromonas caviae, Aeromonas hydrophila and Aeromonas veronii biotype sobria. J Antimicrob Chemother, 49, 701- 702.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Veteriner Cerrahi |
| Diğer ID | JA72NU59DC |
| Bölüm | Araştırma Makalesi |
| Yazarlar | |
| Yayımlanma Tarihi | 1 Mart 2017 |
| Yayımlandığı Sayı | Yıl 2017 |