Ankara Üniversitesi Veteriner Fakültesi Dergisi
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Identification of pituitary-specific transcription factor-1 (PIT-1) and leptin gene (LEP) polymorphism of Holstein cattle reared in Turkey

Yıl 2017, Cilt: 64 Sayı: 4, 337 - 343, 01.12.2017

Davut BAYRAM Korhan ARSLAN Bilal AKYÜZ Kaan Muhsin İŞCAN

https://doi.org/10.1501/Vetfak_0000002818

Öz

Purpose of this study was to examine leptin (LEP) and pituitary-specific transcription factor-1 (Pit-I) gene polymorphisms in Holstein cattle (n=352) in Turkey. In order to determine the Pit-I-HinfI and LEP-Sau3AI polymorphisms, polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) were performed. A 600 bp length fragment of Pit-I and a 422 bp length fragment of LEP were amplified. In this study, two types of alleles, A and B, for both of the Pit-I and LEP genes were observed. The highest frequencies of the alleles were estimated, and were the B allele (0.68) for the Pit-I gene and the A allele (0.87) for the LEP gene. According to the results of the chi-square test, a significant deviation from the Hardy-Weinberg equilibrium was determined for both of these genes in the investigated breed (in the same order; X2:40.75, p<0.001and X2:8.4, p<0.01)

Anahtar Kelimeler

Holstein, leptin, pit-I, polymorphism, RFLP

Kaynakça

  • Almeida S, Almeida E, Moraes J, et al. (2003): Molecular markers in the LEP gene and reproductive performance of beef cattle. J Anim Breed Genet, 120, 106-113
  • Aytekin I, Boztepe S (2013): Associations of Pit-1 gene polymorphism with milk yield and composition traits in Brown Swiss cattle. J Anim Plant Sci, 23, 1281-1289.
  • Block S, Butler W, Bell A, et al. (2001): Decreased concentration of plasma leptin in periparturient dairy cows is caused by negative energy balance. J Endocrinol, 171, 339-348.
  • Buchanan F, Van Kessel A, Waldner C, et al. (2003): Hot topic: An association between a leptin single nucleotide polymorphism and milk and protein yield. J Dairy Sci, 86, 3164-3166.
  • Clempson AM, Pollott GE, Brickell JS, et al. (2011): Evidence that leptin genotype is associated with fertility, growth, and milk production in Holstein cows. J Dairy Sci, 94, 3618-3628.
  • Cohen LE, Wondisford FE, Radovick S (1997): Role of Pit-I in the gene expression of growth hormone, prolactin, and thyrotropin. Endocrinol Metab Clin N Am, 25, 23-540.
  • Cosier V, Vlaic A, Gaboreanu I (2007): HinfI polymorphism of k-casein and Pit1 genes in Romanian Simmental cattle. Sci P Anim Sci Bio, 40, 59-64.
  • Dario C, Selvaggi M, Carnicella D, et al. (2009): Bufano G: STAT5A/Aval polymorphism in Podolica bulls and its effect on growth performance traits. Livest Sci, 123, 83-87.
  • Doosti A, Arshi A, Bomeni B (2011): Molecular study of PIT1 gene polymorphism in Holstein and Iranian native cattle. Ajar, 6, 4467-4470.
  • Dybus A, Szatkowska I, Czerniawska-Piatkowska E, et al. (2004): PIT1-HinfI gene polymorphism and its associations with milk production traits in polish Black- and-White cattle. Arc Tierzucht, 47, 557-564.
  • Edriss MA, Edriss V, Rahmani HR (2009): Association of PIT-1 gene polymorphism with birth weight, milk and reproduction traits in Isfahan Holstein cows. Arch Tierzucht, 52, 445-447.
  • Heidari M, Azari M, Hasani S, et al. (2012): Effect of polymorphic variants of GH, Pit-1, and β-LG genes on milk production of Holstein cows. Russ J Genet, 48, 417-421.
  • Hua GH, Chen SL, Yu JN, et al (2009): Polymorphism of the growth hormone gene and its association with growth traits in Boer goat bucks. Meat Sci, 81, 391-395.
  • Javanmard A, Khaledi K, Asadzadeh N, et al. (2010): Detection of polymorphism in the bovine leptin (LEP) gene: Association of a single nucleotide polymorphism with breeding value of milk traits in Iranian Holstein cattle. J Mol Genet, 2, 10-14.
  • Javanmard A, Mohammadabadi MR, Zarrigabayi GE, et al. (2008): Polymorphism within the intron region of the bovine leptin gene in Iranian Sarabi cattle (Iranian Bos taurus). Russ J Genet, 44, 495-497.
  • Jiang Z, Gibson JP (1999): Genetic polymorphisms in the leptin gene and their association with fatness in four pig breeds. Mamm Genome, 10, 191-193.
  • Kaçar C, Arı UÇ (2007): Leptinin inek ve koyunlarda enerji metabolizması ve üreme fizyolojisi üzerine etkileri. Kafkas Univ Vet Fak, 13, 209-213.
  • Kadokawa H, Blache D, Yamada Y, et al. (2000): Relationships concentrations of leptin before and after parturition and the timing of first post-partum ovulation in high-producing Holstein dairy cows. Reprod Fertil Dev, 12, 405-411.
  • Khatami SR, Lazebnyĭ OE, Maksimenko VF, et al. (2005): Association of DNA polymorphisms of the growth hormone and prolactin genes with milk productivity in Yaroslavl and Black and White cattle. Genetika, 41, 229- 236.
  • Kulig H, Kmiec M (2009): Association between leptin gene polymorphisms and growth traits in Limousin cattle. Russ J Genet, 45, 738-741.
  • Liefers SC, te Pas MF, Veerkamp RF, et al. (2002): Associations between leptin gene polymorphisms and production, live weight, energy balance, feed intake, and fertility in Holstein heifers. J Dairy Sci, 85, 633-1638.
  • Liefers SC, Veerkamp RF, te Pas MF, et al. (2005): Leptin promoter mutations affect leptin levels and performance traits in dairy cows. Anim Genet, 36, 111-118.
  • Litwinczuk Z, Krol J (2002): Polymorphism of main milk proteins in beef cattle maintained in East-Central Poland. Anim Sci Pap Rep, 1, 33-40.
  • Mangalam HJ, Albert VR, Ingraham HA, et al. (1989): A pituitary POU domain protein, Pit-1, activates both growth hormone and prolactin promoters transcriptionally. Gene Dev, 3, 946-958.
  • Mattos KK, Del Lama SN, Martinez ML, et al. (2004): Association of bGH and Pit-1 gene variants with milk production traits in dairy Gyr bulls. Pesq Agropec Bras, 39, 147-150.
  • Moody DE, Pomp D, Barendse W (1995): Restriction fragment length polymorphism in amplification products of the bovine PIT1 gene and assignment of PIT1 to bovine chromosome 1. Anim Genet, 26, 45-47.
  • Moravčíková N, Trakovická A, Kasarda R (2012): Polymorphism within the intron region of the bovine leptin gene in Slovak Pinzgau cattle. J Anim Sci Biotechnol, 45, 211-214.
  • Moussavi A, Ahouei M, Nassiry M, et al. (2006): Association of leptin polymorphism with production, reproduction and plasma glucose level in Iranian Holstein cows. Asian-Aust J Anim Sci, 19, 627-631.
  • Nei M (1978): Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics, 89, 583-590.
  • Oikonomou G, Angelopoulou K, Arsenos G, et al. (2008): The effects of polymorphisms in the DGAT1, leptin and growth hormone receptor gene loci on body energy, blood metabolic and reproductive traits of Holstein cows. Anim Genet, 40, 10-17.
  • Oshima S, Barreras A (2003): Relationships between DGAT1 and Pit-1 genes polymorphism and milk yield in Holstein cattle. J Anim Sci, 54, 1-3.
  • Özdemir M (2011): Genetic polymorphism of leptin gene in Holstein and native East Anatolian Red (EAR)cattle raised as genetic resource in Turkey. Afr J Agric Res, 6, 6008-6010.
  • Passos DT, Hepp D, Moraes JCF, et al. (2007): Effect of polymorphisms linked to LEP gene on its expression on adipose tissues in beef cattle. J Anim Breed Genet, 124, 157-162.
  • Pfaffle RW, Dimattia GE, Parks JS, et al. (1992): Mutation of the Pou-specific domain of Pit-1 and hypopituitarism without pituitary hypoplasia. Science, 257, 1118-1121.
  • Pomp D, Zou T, Clutter AC, Barendse W (1997): Rapid communication: Mapping of leptin to bovine chromosome 4 by linkage analysis of a PCR-based polymorphism. J Anim Sci, 75, 1427.
  • Renaville R, Gengler N, Vrech E, et al. (1997): Pit-1 gene polymorphism, milk yield, and conformation traits for Italian Holstein-Friesian bulls. J Dairy Sci, 80, 3431-3438.
  • Sambrook J, Fritsch E, Maniatis T (1989): Molecular cloning: A laboratory manual, (2nd) ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
  • Santos-Alvarez J, Goberna R, Sanchez-Maralet V (1999): Human activation of human circulating monocytes. Cell Immunol, 194, 6-11. proliferation and
  • Sharifzadeh A, Doosti A (2010): Genetic polymorphism at the leptin gene in Iranian Holstein cattle by PCR-RFLP. J Microbiol Res, 4, 1343-1345.
  • Silva DB, Crispim BA, Silva LE, et al. (2014): Genetic variations in the leptin gene associated with growth and carcass traits in Nellore cattle. Genet Mol Res, 13, 3002- 3012.
  • Unanian MM, Barreto CC, Cordeiro CMT, et al. (2002): Possible associations between bovine growth hormone gene polymorphism and reproductive traits. Braz Arch Biol Techn, 45, 293-299.
  • Viorica C (2006): RFLP/HinfI polymorphism between exon 5 and exon 6 of the PIT-1 gene in Romanian Simmental cattle. Usamv Journal-Cn, 63, 219-222.
  • Yan L, Fang X, Zhang R, et al. (2011): Analysis of pituitary specific transcription factor-1 gene polymorphism in several indigenous Chinese cattle and crossbred cattle. J Appl Anim Res, 39, 269-274.
  • Yang D, Chen H, Wang X, et al. (2007): Association of polymorphisms of leptin gene with body weight and body sizes indexes in Chinese indigenous cattle. J Genet Genomics, 34, 400-405.
  • Yeh FC, Yang R, Boyle T (1999): POPGENE Version 1.31. Microsoft Window-based free software for Population Genetic Analysis, University of Alberta. Edmonton, AB, Canada.
  • Zhang CL, Liu B, Chen H, et al. (2009): Associations of a HinfI PCR-RFLP of POU1F1 gene with growth traits in Qinchuan cattle. Anim Biotechnol, 20, 71-74.
  • Zhang L, Liu J, Zhao F, et al. (2013): Genome-wide association studies for growth and meat production traits in sheep. PloS one, 8, e66569. Geliş tarihi: 05.01.2016 / Kabul tarihi: 14.10.2016 Address for correspondence: Assist. Prof. Dr. Davut BAYRAM Erciyes University, Faculty of Veterinary Medicine, Department of Animal Breeding and Husbandary, Kayseri, Turkey. e-mail: vetdavut@gmail.com

Türkiye’de yetiştirilen Holştayn sığırlarda hipofiz bezine özgü transkripsiyon faktörü- 1 (PIT-1) veleptin (LEP) gen polimorfizmlerinin belirlenmesi

Yıl 2017, Cilt: 64 Sayı: 4, 337 - 343, 01.12.2017

Davut BAYRAM Korhan ARSLAN Bilal AKYÜZ Kaan Muhsin İŞCAN

https://doi.org/10.1501/Vetfak_0000002818

Öz

Bu çalışmada Türkiye’de Kayseri civarında yetiştirilen Holştayn ırkı sığırlarda (n=352) leptin (LEP) ve hipofiz bezine özgü transkripsiyon faktörü -1 (Pit-I) genlerinin polimorfizmlerinin belirlenmesi amaçlanmıştır. Pit-I-HinfI ve LEP-Sau3AI polimorfizmlerinin belirlenmesi amacı ile polimeraz zincir reaksiyonu (PZR) ve restriksiyon parça uzunluk polimorfizm (RFLP) analizi yapılmıştır. Pit-1 geni için 600 bç’lik ve LEP geni için 422 bç’lik uzunluk amplifiye edilmiştir. Çalışmada, Pit-1 ve LEP geni allellerinin her ikisinde de A ve B allelleri olmak üzere iki iki tip allel gözlendi. En yüksek frekans Pit-1 geni için A allelinde (0.87), LEP geni için ise B allelinde (0.68) olarak belirlendi. Ki-kare test sonuçlarına gore, incelenen ırkta, her iki gende de Hardy-Weinberg dengesinden anlamlı bir sapma gözlendi (aynı sıra ile; X2:40.75, p<0.001ve X2:8.4, p<0.01)

Anahtar Kelimeler

Holştayn, leptin, pit-1, polimorfizm, RFLP

Kaynakça

  • Almeida S, Almeida E, Moraes J, et al. (2003): Molecular markers in the LEP gene and reproductive performance of beef cattle. J Anim Breed Genet, 120, 106-113
  • Aytekin I, Boztepe S (2013): Associations of Pit-1 gene polymorphism with milk yield and composition traits in Brown Swiss cattle. J Anim Plant Sci, 23, 1281-1289.
  • Block S, Butler W, Bell A, et al. (2001): Decreased concentration of plasma leptin in periparturient dairy cows is caused by negative energy balance. J Endocrinol, 171, 339-348.
  • Buchanan F, Van Kessel A, Waldner C, et al. (2003): Hot topic: An association between a leptin single nucleotide polymorphism and milk and protein yield. J Dairy Sci, 86, 3164-3166.
  • Clempson AM, Pollott GE, Brickell JS, et al. (2011): Evidence that leptin genotype is associated with fertility, growth, and milk production in Holstein cows. J Dairy Sci, 94, 3618-3628.
  • Cohen LE, Wondisford FE, Radovick S (1997): Role of Pit-I in the gene expression of growth hormone, prolactin, and thyrotropin. Endocrinol Metab Clin N Am, 25, 23-540.
  • Cosier V, Vlaic A, Gaboreanu I (2007): HinfI polymorphism of k-casein and Pit1 genes in Romanian Simmental cattle. Sci P Anim Sci Bio, 40, 59-64.
  • Dario C, Selvaggi M, Carnicella D, et al. (2009): Bufano G: STAT5A/Aval polymorphism in Podolica bulls and its effect on growth performance traits. Livest Sci, 123, 83-87.
  • Doosti A, Arshi A, Bomeni B (2011): Molecular study of PIT1 gene polymorphism in Holstein and Iranian native cattle. Ajar, 6, 4467-4470.
  • Dybus A, Szatkowska I, Czerniawska-Piatkowska E, et al. (2004): PIT1-HinfI gene polymorphism and its associations with milk production traits in polish Black- and-White cattle. Arc Tierzucht, 47, 557-564.
  • Edriss MA, Edriss V, Rahmani HR (2009): Association of PIT-1 gene polymorphism with birth weight, milk and reproduction traits in Isfahan Holstein cows. Arch Tierzucht, 52, 445-447.
  • Heidari M, Azari M, Hasani S, et al. (2012): Effect of polymorphic variants of GH, Pit-1, and β-LG genes on milk production of Holstein cows. Russ J Genet, 48, 417-421.
  • Hua GH, Chen SL, Yu JN, et al (2009): Polymorphism of the growth hormone gene and its association with growth traits in Boer goat bucks. Meat Sci, 81, 391-395.
  • Javanmard A, Khaledi K, Asadzadeh N, et al. (2010): Detection of polymorphism in the bovine leptin (LEP) gene: Association of a single nucleotide polymorphism with breeding value of milk traits in Iranian Holstein cattle. J Mol Genet, 2, 10-14.
  • Javanmard A, Mohammadabadi MR, Zarrigabayi GE, et al. (2008): Polymorphism within the intron region of the bovine leptin gene in Iranian Sarabi cattle (Iranian Bos taurus). Russ J Genet, 44, 495-497.
  • Jiang Z, Gibson JP (1999): Genetic polymorphisms in the leptin gene and their association with fatness in four pig breeds. Mamm Genome, 10, 191-193.
  • Kaçar C, Arı UÇ (2007): Leptinin inek ve koyunlarda enerji metabolizması ve üreme fizyolojisi üzerine etkileri. Kafkas Univ Vet Fak, 13, 209-213.
  • Kadokawa H, Blache D, Yamada Y, et al. (2000): Relationships concentrations of leptin before and after parturition and the timing of first post-partum ovulation in high-producing Holstein dairy cows. Reprod Fertil Dev, 12, 405-411.
  • Khatami SR, Lazebnyĭ OE, Maksimenko VF, et al. (2005): Association of DNA polymorphisms of the growth hormone and prolactin genes with milk productivity in Yaroslavl and Black and White cattle. Genetika, 41, 229- 236.
  • Kulig H, Kmiec M (2009): Association between leptin gene polymorphisms and growth traits in Limousin cattle. Russ J Genet, 45, 738-741.
  • Liefers SC, te Pas MF, Veerkamp RF, et al. (2002): Associations between leptin gene polymorphisms and production, live weight, energy balance, feed intake, and fertility in Holstein heifers. J Dairy Sci, 85, 633-1638.
  • Liefers SC, Veerkamp RF, te Pas MF, et al. (2005): Leptin promoter mutations affect leptin levels and performance traits in dairy cows. Anim Genet, 36, 111-118.
  • Litwinczuk Z, Krol J (2002): Polymorphism of main milk proteins in beef cattle maintained in East-Central Poland. Anim Sci Pap Rep, 1, 33-40.
  • Mangalam HJ, Albert VR, Ingraham HA, et al. (1989): A pituitary POU domain protein, Pit-1, activates both growth hormone and prolactin promoters transcriptionally. Gene Dev, 3, 946-958.
  • Mattos KK, Del Lama SN, Martinez ML, et al. (2004): Association of bGH and Pit-1 gene variants with milk production traits in dairy Gyr bulls. Pesq Agropec Bras, 39, 147-150.
  • Moody DE, Pomp D, Barendse W (1995): Restriction fragment length polymorphism in amplification products of the bovine PIT1 gene and assignment of PIT1 to bovine chromosome 1. Anim Genet, 26, 45-47.
  • Moravčíková N, Trakovická A, Kasarda R (2012): Polymorphism within the intron region of the bovine leptin gene in Slovak Pinzgau cattle. J Anim Sci Biotechnol, 45, 211-214.
  • Moussavi A, Ahouei M, Nassiry M, et al. (2006): Association of leptin polymorphism with production, reproduction and plasma glucose level in Iranian Holstein cows. Asian-Aust J Anim Sci, 19, 627-631.
  • Nei M (1978): Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics, 89, 583-590.
  • Oikonomou G, Angelopoulou K, Arsenos G, et al. (2008): The effects of polymorphisms in the DGAT1, leptin and growth hormone receptor gene loci on body energy, blood metabolic and reproductive traits of Holstein cows. Anim Genet, 40, 10-17.
  • Oshima S, Barreras A (2003): Relationships between DGAT1 and Pit-1 genes polymorphism and milk yield in Holstein cattle. J Anim Sci, 54, 1-3.
  • Özdemir M (2011): Genetic polymorphism of leptin gene in Holstein and native East Anatolian Red (EAR)cattle raised as genetic resource in Turkey. Afr J Agric Res, 6, 6008-6010.
  • Passos DT, Hepp D, Moraes JCF, et al. (2007): Effect of polymorphisms linked to LEP gene on its expression on adipose tissues in beef cattle. J Anim Breed Genet, 124, 157-162.
  • Pfaffle RW, Dimattia GE, Parks JS, et al. (1992): Mutation of the Pou-specific domain of Pit-1 and hypopituitarism without pituitary hypoplasia. Science, 257, 1118-1121.
  • Pomp D, Zou T, Clutter AC, Barendse W (1997): Rapid communication: Mapping of leptin to bovine chromosome 4 by linkage analysis of a PCR-based polymorphism. J Anim Sci, 75, 1427.
  • Renaville R, Gengler N, Vrech E, et al. (1997): Pit-1 gene polymorphism, milk yield, and conformation traits for Italian Holstein-Friesian bulls. J Dairy Sci, 80, 3431-3438.
  • Sambrook J, Fritsch E, Maniatis T (1989): Molecular cloning: A laboratory manual, (2nd) ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
  • Santos-Alvarez J, Goberna R, Sanchez-Maralet V (1999): Human activation of human circulating monocytes. Cell Immunol, 194, 6-11. proliferation and
  • Sharifzadeh A, Doosti A (2010): Genetic polymorphism at the leptin gene in Iranian Holstein cattle by PCR-RFLP. J Microbiol Res, 4, 1343-1345.
  • Silva DB, Crispim BA, Silva LE, et al. (2014): Genetic variations in the leptin gene associated with growth and carcass traits in Nellore cattle. Genet Mol Res, 13, 3002- 3012.
  • Unanian MM, Barreto CC, Cordeiro CMT, et al. (2002): Possible associations between bovine growth hormone gene polymorphism and reproductive traits. Braz Arch Biol Techn, 45, 293-299.
  • Viorica C (2006): RFLP/HinfI polymorphism between exon 5 and exon 6 of the PIT-1 gene in Romanian Simmental cattle. Usamv Journal-Cn, 63, 219-222.
  • Yan L, Fang X, Zhang R, et al. (2011): Analysis of pituitary specific transcription factor-1 gene polymorphism in several indigenous Chinese cattle and crossbred cattle. J Appl Anim Res, 39, 269-274.
  • Yang D, Chen H, Wang X, et al. (2007): Association of polymorphisms of leptin gene with body weight and body sizes indexes in Chinese indigenous cattle. J Genet Genomics, 34, 400-405.
  • Yeh FC, Yang R, Boyle T (1999): POPGENE Version 1.31. Microsoft Window-based free software for Population Genetic Analysis, University of Alberta. Edmonton, AB, Canada.
  • Zhang CL, Liu B, Chen H, et al. (2009): Associations of a HinfI PCR-RFLP of POU1F1 gene with growth traits in Qinchuan cattle. Anim Biotechnol, 20, 71-74.
  • Zhang L, Liu J, Zhao F, et al. (2013): Genome-wide association studies for growth and meat production traits in sheep. PloS one, 8, e66569. Geliş tarihi: 05.01.2016 / Kabul tarihi: 14.10.2016 Address for correspondence: Assist. Prof. Dr. Davut BAYRAM Erciyes University, Faculty of Veterinary Medicine, Department of Animal Breeding and Husbandary, Kayseri, Turkey. e-mail: vetdavut@gmail.com

Ayrıntılar

Birincil Dil İngilizce
Konular Veteriner Cerrahi
Diğer ID JA59GT96VH
Bölüm Araştırma Makalesi
Yazarlar

Davut BAYRAM
Erciyes University, Department of Animal Breeding and Husbandary, Faculty of Veterinary Medicine;

Korhan ARSLAN
Erciyes University, Department of Animal Breeding and Husbandary, Faculty of Veterinary Medicine;

Bilal AKYÜZ
Erciyes University, Department of Animal Breeding and Husbandary, Faculty of Veterinary Medicine;

Kaan Muhsin İŞCAN
Erciyes University, Department of Animal Breeding and Husbandary, Faculty of Veterinary Medicine;

Yayımlanma Tarihi 1 Aralık 2017
Yayınlandığı Sayı Yıl 2017Cilt: 64 Sayı: 4

Kaynak Göster

Bibtex @editöre mektup { auvfd525967, journal = {Ankara Üniversitesi Veteriner Fakültesi Dergisi}, issn = {1300-0861}, eissn = {1308-2817}, address = {}, publisher = {Ankara Üniversitesi}, year = {2017}, volume = {64}, number = {4}, pages = {337 - 343}, doi = {10.1501/Vetfak\_0000002818}, title = {Identification of pituitary-specific transcription factor-1 (PIT-1) and leptin gene (LEP) polymorphism of Holstein cattle reared in Turkey}, key = {cite}, author = {Bayram, Davut and Arslan, Korhan and Akyüz, Bilal and İşcan, Kaan Muhsin} }
APA Bayram, D. , Arslan, K. , Akyüz, B. & İşcan, K. M. (2017). Identification of pituitary-specific transcription factor-1 (PIT-1) and leptin gene (LEP) polymorphism of Holstein cattle reared in Turkey . Ankara Üniversitesi Veteriner Fakültesi Dergisi , 64 (4) , 337-343 . DOI: 10.1501/Vetfak_0000002818
MLA Bayram, D. , Arslan, K. , Akyüz, B. , İşcan, K. M. "Identification of pituitary-specific transcription factor-1 (PIT-1) and leptin gene (LEP) polymorphism of Holstein cattle reared in Turkey" . Ankara Üniversitesi Veteriner Fakültesi Dergisi 64 (2017 ): 337-343 <http://vetjournal.ankara.edu.tr/tr/pub/issue/43284/525967>
Chicago Bayram, D. , Arslan, K. , Akyüz, B. , İşcan, K. M. "Identification of pituitary-specific transcription factor-1 (PIT-1) and leptin gene (LEP) polymorphism of Holstein cattle reared in Turkey". Ankara Üniversitesi Veteriner Fakültesi Dergisi 64 (2017 ): 337-343
RIS TY - JOUR T1 - Türkiye’de yetiştirilen Holştayn sığırlarda hipofiz bezine özgü transkripsiyon faktörü- 1 (PIT-1) veleptin (LEP) gen polimorfizmlerinin belirlenmesi AU - DavutBayram, KorhanArslan, BilalAkyüz, Kaan Muhsinİşcan Y1 - 2017 PY - 2017 N1 - doi: 10.1501/Vetfak_0000002818 DO - 10.1501/Vetfak_0000002818 T2 - Ankara Üniversitesi Veteriner Fakültesi Dergisi JF - Journal JO - JOR SP - 337 EP - 343 VL - 64 IS - 4 SN - 1300-0861-1308-2817 M3 - doi: 10.1501/Vetfak_0000002818 UR - https://doi.org/10.1501/Vetfak_0000002818 Y2 - 2023 ER -
EndNote %0 Ankara Üniversitesi Veteriner Fakültesi Dergisi Identification of pituitary-specific transcription factor-1 (PIT-1) and leptin gene (LEP) polymorphism of Holstein cattle reared in Turkey %A Davut Bayram , Korhan Arslan , Bilal Akyüz , Kaan Muhsin İşcan %T Identification of pituitary-specific transcription factor-1 (PIT-1) and leptin gene (LEP) polymorphism of Holstein cattle reared in Turkey %D 2017 %J Ankara Üniversitesi Veteriner Fakültesi Dergisi %P 1300-0861-1308-2817 %V 64 %N 4 %R doi: 10.1501/Vetfak_0000002818 %U 10.1501/Vetfak_0000002818
ISNAD Bayram, Davut , Arslan, Korhan , Akyüz, Bilal , İşcan, Kaan Muhsin . "Identification of pituitary-specific transcription factor-1 (PIT-1) and leptin gene (LEP) polymorphism of Holstein cattle reared in Turkey". Ankara Üniversitesi Veteriner Fakültesi Dergisi 64 / 4 (Aralık 2017): 337-343 . https://doi.org/10.1501/Vetfak_0000002818
AMA Bayram D. , Arslan K. , Akyüz B. , İşcan K. M. Identification of pituitary-specific transcription factor-1 (PIT-1) and leptin gene (LEP) polymorphism of Holstein cattle reared in Turkey. Ankara Univ Vet Fak Derg. 2017; 64(4): 337-343.
Vancouver Bayram D. , Arslan K. , Akyüz B. , İşcan K. M. Identification of pituitary-specific transcription factor-1 (PIT-1) and leptin gene (LEP) polymorphism of Holstein cattle reared in Turkey. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2017; 64(4): 337-343.
IEEE D. Bayram , K. Arslan , B. Akyüz ve K. M. İşcan , "Identification of pituitary-specific transcription factor-1 (PIT-1) and leptin gene (LEP) polymorphism of Holstein cattle reared in Turkey", Ankara Üniversitesi Veteriner Fakültesi Dergisi, c. 64, sayı. 4, ss. 337-343, Ara. 2017, doi:10.1501/Vetfak_0000002818