Ankara Üniversitesi Veteriner Fakültesi Dergisi
 
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Genç ve yaşlı köpeklerin yağ dokusundan elde edilen mezenkimal kök hücrelerin karakterizasyonu

Yıl 2016, Cilt: 63 Sayı: 3, 297 - 302, 01.09.2016

Sancak İrem GÜL Asuman ÖZEN Alev Gürol BAYRAKTAROĞLU Ahmet CEYLAN Pınar CAN

Öz

Mezenkimal kök hücrelerin veteriner hekimlik alanında kullanımı birçok hastalığın tedavisi için umut ışığı olarak görülmektedir. Yağ doku kökenli mezenkimal kök hücrelerin eldesinin kolay olması ve üretiminin ek bir maliyet getirmemesi sebebiyle yağ dokudan kök hücre üretimi en çok kullanılan yöntem olarak karşımıza çıkmaktadır. Yağ doku kökenli hücrelerin multilineage potansiyeline sahip olduğu bilinmektedir. Köpek yağ doku kaynaklı mezenkimal kök hücrelerin başarılı bir şekilde transplante edildiği de daha önceki çalışmalarda da bildirilmiştir. Bu çalışmada 3 adet yaşlı (5 yaş üzeri) ve 3 adet genç (6 ay ve altı) köpekten operasyon sırasında alınan yağ doku örneklerinden elde edilen mezenkimal kök hücrelerin karşılaştırılması, adipojenik, kondrojenik ve osteojenik farklılaşma potansiyeli, farklılaşma hızı, üreme hızı ve karakteristik özellikleri yönünden aralarındaki farkların ortaya konulması, lipoaspiratın içerdiği kök hücre potansiyelinin karşılaştırılması amaçlanmıştır

Anahtar Kelimeler

Farklılaşma, köpek, mezenkimal kök hücre, yağ doku

Kaynakça

  • Can A (2014): Kök hücre. Akademisyen Tıp Kitabevi, Yenişehir, Ankara.
  • Csaki C, Matis U, Mobasheri A, et al. (2007): Chondrogenesis, osteogenesis and adipogenesis of canine mesenchymal stem cells: a biochemical, morphological and ultrastructural study. Histochem Cell Biol, 128, 507- 20.
  • Diaz-Romero J, Quintin A, Schoenholzer E, et al. (2013): S100A1 and S100B Expression Patterns Identify Differentiation Status of Human Articular Chondrocytes. J Cell Physiol, 229, 1106-1117.
  • Gartner LP, Hiatt JL (1997): Colour textbook of histology. WB Sounders Company, Philadelphia, Pensylvania.
  • Gül Sancak İ, Özen A, Alparslan Pınarlı F, et al. (2014): Limbal Stem Cells in dogs and cats their identification culture and differentiation into keratinocytes. Kafkas Univ Vet Fak Derg, 20, 909-914.
  • Halvorsen YD, Bond A, Sen A, et al. (2001): Thiazolidinediones and glucocorticoids induce differentiation of human adipose tissue stromal cells: Biochemical, cellular, and molecular analysis. Metabolism, 50, 407-413. synergistically
  • Awad HA, Butler DL, Boivin GP, et al. (1999): Autologous Mesenchymal Stem Cell-Mediated Repair of Tendon. Tissue Engineering, 5, 267-277.
  • Justesen J, Pedersen SB, Stenderup K, et al. (2004): Subcutaneous adipocytes can differentiate into bone- forming cells in vitro and in vivo. Tissue Eng, 10, 381-391.
  • Karaöz E, Aksoy A, Ayhan S, et al. (2009): Characterization of mesenchymal stem cells from rat bone marrow: potential and immunophenotypic markers. Histochem Cell Biol, 132, 533-546. properties, differentiation
  • Lettry V, Hosoya K, Takagi S, et al. (2010): Coculture of equine mesenchymal stem cells and mature equine articular chondrocytes results in improved chondrogenic differentiation of the stem cells. Jpn J Vet Res, 58, 5-15.
  • Meliga E, Strem BM, Duckers HJ, et al. (2007): Adipose-derived cells. Cell Trans, 16, 963-970.
  • Miranville A, Heeschen C, Sengenes C, et al. (2002): Improvement of postnatal neovascularization by human adipose tissue-derived stem cells. Circulation, 110, 349- 355.
  • Mizuno H, Zuk PA, Zhu M, et al. (2002): Myogenic differentiation by human processed lipoaspirate cells. Plast Reconstr Surg, 109, 199-209.
  • Orbay H, Tobita M, Mizuno H (2012): Mesenchymal Stem Cells Isolated from Adipose and Other Tissues: Basic Biological Properties and Clinical Applications. Stem Cells International Volume, Article ID 461718, 9. http://dx.doi.org/10.1155/2012/461718.
  • Ozen A, Gul Sancak I, Koch S, et al. (2013): Ultrastructural characteristics of sheep and horse mesenchymal stem cells (MSCs). Mic Res, 1, 17-23.
  • Özen A, Gül Sancak İ (2014): Mezenkimal kök hücreler ve veteriner hekimlikte kullanımı. Ankara Üniv Vet Fak Derg, 61, 79-84.
  • Özen A, Gül Sancak İ, Tiryaki M, et al. (2013): Mesenchymal stem cells (Mscs) in scanning electron microscopy (SEM) world. Niche, 2: 22-4. DOI: 10.5152/niche.2013.162
  • Planat-Benard V, Silvestre J S, Cousin B, et al. (2004): Plasticity of human adipose lineage cells toward endothelial perspectives. Circulation, 109, 656-663. and therapeutic
  • Rehman J, Traktuev D, Li J, et al. (2004): Secretion of angiogenic and antiapoptotic factors by human adipose stromal cells. Circulation, 109, 1292.
  • Safford KM, Hicok KC, Safford SD, et al. (2002): Neurogenic differentiation of murine and human adipose- derived stromal cells. Biochem Biophys Res Commun, 294, 371-379.
  • Sarraf CE, Otto WR, Eastwood M (2011): In vitro mesenchymal stem cell differentiation after mechanical stimulation. Cell Prolif. Feb, 44, 99-108.
  • Seo MJ, Suh SY, Bae YC, et al. (2005): Differentiation of human adipose stromal cells into hepatic lineage in vitro and in vivo. Biochem. Biophys. Res. Commun, 328, 258- 264.
  • Vieira NM, Brandalise V, Zucconi E, et al. (2010): Isolation, Characterization, and Differentiation Potential of Canine Adipose-Derived Stem Cells. Cell Transplantation, 19, 279-289.
  • Yamamoto N, Akamatsu H, Hasegawa S, et al. (2007): Isolation of multipotent stem cells from mouse adipose tissue. J Dermatol Sci, 48, 43-52.
  • Zuk PA, Zhu M, Mizuno H, et al. (2001): Multilineage cells from human adipose tissue: implications for cell- based therapies. Tissue Eng, 7, 211-28. Geliş tarihi: 24.04.2015 / Kabul tarihi: 27.11.2015 Address for correspondence: Prof. Dr. Asuman Özen Ankara University, Faculty of Veterinary Medicine, Department of Histology and Embryology, 06110 Dışkapı, Ankara-Turkey. e-mail: ozen@veterinary.ankara.edu.tr

Characterization of mesenchymal stem cells isolated from the adipose tissue of young and old dogs

Yıl 2016, Cilt: 63 Sayı: 3, 297 - 302, 01.09.2016

Sancak İrem GÜL Asuman ÖZEN Alev Gürol BAYRAKTAROĞLU Ahmet CEYLAN Pınar CAN

Öz

The use of mesenchymal cells in veterinary medicine is considered to be promising for the treatment of several diseases. Due to adipose mesenchymal stem cells being readily available and the expansion of these cells not incurring any additional cost, the culturing of adipose mesenchymal cells has become a commonly applied method. Adipose mesenchymal cells are known to have a multilineage potential of differentiation. Previous research has demonstrated success in the transplantation of canine adipose mesenchymal cells. This study was aimed at the comparative evaluation of mesenchymal stem cells isolated from adipose tissue samples taken at surgery from 3 old dogs (above 5 years of age) and 3 young dogs (1 to 6-months-old or younger), and at the determination of any differences in the adipogenic, chondrogenic, and osteogenic differentiation potential, differentiation rate, proliferation rate, and characteristics of these cells. Furthermore, a comparison was made for the stem cell potential of the lipoaspirates

Anahtar Kelimeler

Adipose tissue, differentiation, dog, mesenchymal stem cell

Kaynakça

  • Can A (2014): Kök hücre. Akademisyen Tıp Kitabevi, Yenişehir, Ankara.
  • Csaki C, Matis U, Mobasheri A, et al. (2007): Chondrogenesis, osteogenesis and adipogenesis of canine mesenchymal stem cells: a biochemical, morphological and ultrastructural study. Histochem Cell Biol, 128, 507- 20.
  • Diaz-Romero J, Quintin A, Schoenholzer E, et al. (2013): S100A1 and S100B Expression Patterns Identify Differentiation Status of Human Articular Chondrocytes. J Cell Physiol, 229, 1106-1117.
  • Gartner LP, Hiatt JL (1997): Colour textbook of histology. WB Sounders Company, Philadelphia, Pensylvania.
  • Gül Sancak İ, Özen A, Alparslan Pınarlı F, et al. (2014): Limbal Stem Cells in dogs and cats their identification culture and differentiation into keratinocytes. Kafkas Univ Vet Fak Derg, 20, 909-914.
  • Halvorsen YD, Bond A, Sen A, et al. (2001): Thiazolidinediones and glucocorticoids induce differentiation of human adipose tissue stromal cells: Biochemical, cellular, and molecular analysis. Metabolism, 50, 407-413. synergistically
  • Awad HA, Butler DL, Boivin GP, et al. (1999): Autologous Mesenchymal Stem Cell-Mediated Repair of Tendon. Tissue Engineering, 5, 267-277.
  • Justesen J, Pedersen SB, Stenderup K, et al. (2004): Subcutaneous adipocytes can differentiate into bone- forming cells in vitro and in vivo. Tissue Eng, 10, 381-391.
  • Karaöz E, Aksoy A, Ayhan S, et al. (2009): Characterization of mesenchymal stem cells from rat bone marrow: potential and immunophenotypic markers. Histochem Cell Biol, 132, 533-546. properties, differentiation
  • Lettry V, Hosoya K, Takagi S, et al. (2010): Coculture of equine mesenchymal stem cells and mature equine articular chondrocytes results in improved chondrogenic differentiation of the stem cells. Jpn J Vet Res, 58, 5-15.
  • Meliga E, Strem BM, Duckers HJ, et al. (2007): Adipose-derived cells. Cell Trans, 16, 963-970.
  • Miranville A, Heeschen C, Sengenes C, et al. (2002): Improvement of postnatal neovascularization by human adipose tissue-derived stem cells. Circulation, 110, 349- 355.
  • Mizuno H, Zuk PA, Zhu M, et al. (2002): Myogenic differentiation by human processed lipoaspirate cells. Plast Reconstr Surg, 109, 199-209.
  • Orbay H, Tobita M, Mizuno H (2012): Mesenchymal Stem Cells Isolated from Adipose and Other Tissues: Basic Biological Properties and Clinical Applications. Stem Cells International Volume, Article ID 461718, 9. http://dx.doi.org/10.1155/2012/461718.
  • Ozen A, Gul Sancak I, Koch S, et al. (2013): Ultrastructural characteristics of sheep and horse mesenchymal stem cells (MSCs). Mic Res, 1, 17-23.
  • Özen A, Gül Sancak İ (2014): Mezenkimal kök hücreler ve veteriner hekimlikte kullanımı. Ankara Üniv Vet Fak Derg, 61, 79-84.
  • Özen A, Gül Sancak İ, Tiryaki M, et al. (2013): Mesenchymal stem cells (Mscs) in scanning electron microscopy (SEM) world. Niche, 2: 22-4. DOI: 10.5152/niche.2013.162
  • Planat-Benard V, Silvestre J S, Cousin B, et al. (2004): Plasticity of human adipose lineage cells toward endothelial perspectives. Circulation, 109, 656-663. and therapeutic
  • Rehman J, Traktuev D, Li J, et al. (2004): Secretion of angiogenic and antiapoptotic factors by human adipose stromal cells. Circulation, 109, 1292.
  • Safford KM, Hicok KC, Safford SD, et al. (2002): Neurogenic differentiation of murine and human adipose- derived stromal cells. Biochem Biophys Res Commun, 294, 371-379.
  • Sarraf CE, Otto WR, Eastwood M (2011): In vitro mesenchymal stem cell differentiation after mechanical stimulation. Cell Prolif. Feb, 44, 99-108.
  • Seo MJ, Suh SY, Bae YC, et al. (2005): Differentiation of human adipose stromal cells into hepatic lineage in vitro and in vivo. Biochem. Biophys. Res. Commun, 328, 258- 264.
  • Vieira NM, Brandalise V, Zucconi E, et al. (2010): Isolation, Characterization, and Differentiation Potential of Canine Adipose-Derived Stem Cells. Cell Transplantation, 19, 279-289.
  • Yamamoto N, Akamatsu H, Hasegawa S, et al. (2007): Isolation of multipotent stem cells from mouse adipose tissue. J Dermatol Sci, 48, 43-52.
  • Zuk PA, Zhu M, Mizuno H, et al. (2001): Multilineage cells from human adipose tissue: implications for cell- based therapies. Tissue Eng, 7, 211-28. Geliş tarihi: 24.04.2015 / Kabul tarihi: 27.11.2015 Address for correspondence: Prof. Dr. Asuman Özen Ankara University, Faculty of Veterinary Medicine, Department of Histology and Embryology, 06110 Dışkapı, Ankara-Turkey. e-mail: ozen@veterinary.ankara.edu.tr

Ayrıntılar

Diğer ID JA32AR38VT
Bölüm Araştırma Makalesi
Yazarlar

Sancak İrem GÜL

Asuman ÖZEN

Alev Gürol BAYRAKTAROĞLU

Ahmet CEYLAN

Pınar CAN

Yayımlanma Tarihi 1 Eylül 2016
Yayınlandığı Sayı Yıl 2016Cilt: 63 Sayı: 3

Kaynak Göster

Bibtex @ { auvfd526312, journal = {Ankara Üniversitesi Veteriner Fakültesi Dergisi}, issn = {1300-0861}, eissn = {1308-2817}, address = {}, publisher = {Ankara Üniversitesi}, year = {2016}, volume = {63}, number = {3}, pages = {297 - 302}, title = {Characterization of mesenchymal stem cells isolated from the adipose tissue of young and old dogs}, key = {cite}, author = {Gül, Sancak İrem and Özen, Asuman and Bayraktaroğlu, Alev Gürol and Ceylan, Ahmet and Can, Pınar} }
APA Gül, S. İ. , Özen, A. , Bayraktaroğlu, A. G. , Ceylan, A. & Can, P. (2016). Characterization of mesenchymal stem cells isolated from the adipose tissue of young and old dogs . Ankara Üniversitesi Veteriner Fakültesi Dergisi , 63 (3) , 297-302 . Retrieved from http://vetjournal.ankara.edu.tr/tr/pub/issue/43296/526312
MLA Gül, S. İ. , Özen, A. , Bayraktaroğlu, A. G. , Ceylan, A. , Can, P. "Characterization of mesenchymal stem cells isolated from the adipose tissue of young and old dogs" . Ankara Üniversitesi Veteriner Fakültesi Dergisi 63 (2016 ): 297-302 <http://vetjournal.ankara.edu.tr/tr/pub/issue/43296/526312>
Chicago Gül, S. İ. , Özen, A. , Bayraktaroğlu, A. G. , Ceylan, A. , Can, P. "Characterization of mesenchymal stem cells isolated from the adipose tissue of young and old dogs". Ankara Üniversitesi Veteriner Fakültesi Dergisi 63 (2016 ): 297-302
RIS TY - JOUR T1 - Genç ve yaşlı köpeklerin yağ dokusundan elde edilen mezenkimal kök hücrelerin karakterizasyonu AU - Sancak İremGül, AsumanÖzen, Alev GürolBayraktaroğlu, AhmetCeylan, PınarCan Y1 - 2016 PY - 2016 N1 - DO - T2 - Ankara Üniversitesi Veteriner Fakültesi Dergisi JF - Journal JO - JOR SP - 297 EP - 302 VL - 63 IS - 3 SN - 1300-0861-1308-2817 M3 - UR - Y2 - 2023 ER -
EndNote %0 Ankara Üniversitesi Veteriner Fakültesi Dergisi Characterization of mesenchymal stem cells isolated from the adipose tissue of young and old dogs %A Sancak İrem Gül , Asuman Özen , Alev Gürol Bayraktaroğlu , Ahmet Ceylan , Pınar Can %T Characterization of mesenchymal stem cells isolated from the adipose tissue of young and old dogs %D 2016 %J Ankara Üniversitesi Veteriner Fakültesi Dergisi %P 1300-0861-1308-2817 %V 63 %N 3 %R %U
ISNAD Gül, Sancak İrem , Özen, Asuman , Bayraktaroğlu, Alev Gürol , Ceylan, Ahmet , Can, Pınar . "Characterization of mesenchymal stem cells isolated from the adipose tissue of young and old dogs". Ankara Üniversitesi Veteriner Fakültesi Dergisi 63 / 3 (Eylül 2016): 297-302 .
AMA Gül S. İ. , Özen A. , Bayraktaroğlu A. G. , Ceylan A. , Can P. Characterization of mesenchymal stem cells isolated from the adipose tissue of young and old dogs. Ankara Univ Vet Fak Derg. 2016; 63(3): 297-302.
Vancouver Gül S. İ. , Özen A. , Bayraktaroğlu A. G. , Ceylan A. , Can P. Characterization of mesenchymal stem cells isolated from the adipose tissue of young and old dogs. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2016; 63(3): 297-302.
IEEE S. İ. Gül , A. Özen , A. G. Bayraktaroğlu , A. Ceylan ve P. Can , "Characterization of mesenchymal stem cells isolated from the adipose tissue of young and old dogs", Ankara Üniversitesi Veteriner Fakültesi Dergisi, c. 63, sayı. 3, ss. 297-302, Eyl. 2016