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Coxiella burnetii’nin kenelerden Polimeraz Zincir Reaksiyonu ve Restriction Fragment LengthPolymorphism ile saptanması

Yıl 2013, Cilt: 60 Sayı: 4, 263 - 268, 01.12.2013
https://doi.org/10.1501/Vetfak_0000002590

Öz

Coxiella burnetii’nin teşhisi amacıyla Türkiye’nin 38 ilinden toplam 2472 (1446 dişi, 1021 erkek, 5 nymph) adet kene toplandı. Keneler toplandıkları il, tür ve cinsiyetlerine göre 1-7 adedi bir araya getirilerek gruplandırıldı. Gruplar DNA ekstraksiyonu sonrasında CB1 ve CB2 primerleri kullanılarak PCR ile C. burnetii’nin varlığı yönünde incelendi. Denizli’ye ait 6 grup (Toplam 56 kene ve 13 grup) ile Ankara’ya ait bir grup (Toplam 160 kene ve 53 grup) C. burnetii yönünden pozitif bulundu. Rhipicephalus turanicus, Rhipicephalus bursa ve Hyalomma excavatum türlerinin C. burnetii taşıdıkları belirlendi. Cinsiyetin etkenin taşınmasında etkili olmadığı görüldü. PCR ürünlerinin spesifitesi restriction fragment length polimorphism (RFLP) analizi ile değerlendirildi. Pozitif PCR ürünleri TaqІ enzimi ile kesilerek, C. burnetii Nine Mile RSA493 suşunda görüldüğü gibi, 118, 57, 43 ve 39 bp’lik 4 bant ortaya konuldu

Kaynakça

  • Aitken, I.D., Bögel, K., Cracea, E. et al. (1987) Q Fever in Europe: Current aspects of aetiology, epidemiology, human infection, diagnosis and therapy. Infection, 15,323
  • Akgün, E., Yılmaz, M., Pınarbaşı, E. (2006) Q-fever şüphesi olan hasta serum ve kan örneklerinde nested-PCR yöntemiyle Coxiella burnetii’nin saptanması. Cumhuriyet Üniv Tıp Fak Derg, 28, 50-54.
  • Bernasconi, M.V., Casati, S., Peter, O., Piffaretti, J.C. (2002) Rhipicephalus ticks infected with Rickettsia and Coxiella in Southern Switzerland (Canton Ticino). Infect Genet Evol, 2,111-120.
  • Çetinkaya, B., Kalender, H., Ertaş, H.B. et al. (2000) Seroprevalence of coxiellosis in cattle, sheep and people in the east of Turkey. Vet Rec, 146, 131-136.
  • Fard, S.R.N. and Khalili, M. (2011) PCR detection of Coxiella burnetii in ticks collected from sheep and goats in southeast Iran. Iran J Arthropod-Borne Dis, 5,1-6.
  • Fournier, P.E., Marrie, T.J., Raoult, D. (1998) Diagnosis of Q-Fever. J Clin Microbiol, 36, 1823-1834.
  • Higgins, J.A., Azad, A.F. (1995) Use of polymerase chain reaction to detect bacteria in arthropods: a review. J Med Entomol, 32, 213-222.
  • Houver, A.T., Vodkin, M.H., Williams, J.C. (1992) A Coxiella burnetii repeated DNA element resembling a bacterial insertion sequence. J Bacteriol, 174, 5540-5548.
  • Inokuma, H., Yoshizaki, Y., Shimada, Y. et al. (2003) Epidemiological survey of Babesia species in Japan performed with specimens from ticks collected from dogs and detection of new Babesia DNA closely related to Babesia odocoilei and Babesia divergens DNA. J Clin Microbiol, 41, 3494-3498.
  • Kenneth, L.G., Schrumpf, M.E., Karstens, R.H. et al. (1994) DNA typing of Rickettsiae in naturally infected ticks using polymerase chain reaction/restriction fragment length polymorphism system. Am J Trop Med Hyg, 50, 247-260.
  • Kılıç, S ve Çelebi, B. (2008) Türkiye’de C. burnetii’nin epidemiyolojisi (2. Bölüm). Türk Hij Den Biyol Derg, 65 S3, 21-31.
  • Kırkan, Ş., Kaya, O., Tekbıyık, S. and Parın, U. (2008) Detection of Coxiella burnetii in cattle by PCR. Turk J Vet Anim Sci, 32, 215-220.
  • Lee, J.H., Park, H.S., Jang, W.J. et al. (2004) Identification of the Coxiella sp. detected from Haemaphysalis longicornis ticks in Korea. Microbiol Immunol, 48,125-130.
  • Masala, G., Porcu, R., Sana, G. et al. (2004) Occurence, distribution, and role in abortion of Coxiella burnetii in sheep and goats in Sardinia, Italy. Vet Microbiol, 99, 301- 302.
  • Maurin, M. and Raoult, D. (1999) Q Fever. Clin Microbiol Rev, 12, 518-553.
  • Mediannikov, O., Fenollar, F., Socolovschi, C. et al. (2010) Coxiella burnetii in Humans and ticks in rural Senegal. PLOS Negl Trop Dis, 4, e654, doi:10.1371/journal.pntd.0000654
  • Nguyen, S.V. and Hirai, K. (1999) Differentiation of Coxiella burnetii isolates by sequence determination and PCR-restriction fragment length polymorphism analysis of isocitrate dehydrogenase gene. FEMS Microbiol Lett, 180, 249-254.
  • Öngör, H., Çetinkaya, B., Karahan, M. et al. (2004) Detection of Coxiella burnetii by immunomagnetic separation-PCR in the milk of sheep in Turkey. Vet Rec, 154, 570-572.
  • Özgür, N.Y., Haköksüz, M., Yılmaz, H. et al. (1997) İnfertilite sorunu olan dişi sığırlarda ve insanlarda Coxiella burnetii antikorlarının ELISA testi ile belirlenmesi ve seroprevalansının saptanması. Pendik Vet Mikrobiyol Derg, 28, 207-217.
  • Özyer, M., Miroğlu, M., Köksal, F. (1990) Çukurova bölgesinde yaşayan insan ve hayvanlarda Q fever infeksiyonu insidansının komplement fiksasyon testi ile araştırılması. Pendik Hay Hast Merk Araşt Enst Derg, 21, 28-39.
  • Parola, P. and Raoult, D. (2001) Tick-borne bacterial diseases emerging in Europe. Clin Microbiol Infect, 7, 80- 83.
  • Psaroulaki, A., Ragiadakou, D., Kouris, G. et al. (2006) Ticks, tick-borne Rickettsiae, and Coxiella burnetii in the Greek Island of Cephalonia. Ann N Y Acad Sci, 1078, 389-399.
  • Rehacek, J. (1987) Epidemiology and significance of Q Fever in Czechoslovakia. Zentralbl Bakteriol Microbiol Hyg A, 267,16-19.
  • Rehacek, J, Urvölgyi, J., Kocianova, E. et al. (1991) Extensive examination of different tick species for infestation with Coxiella burnetii in Slovakia. Eur J Epidemiol, 7, 299-303.
  • Reinthaler, F.F., Mascher, F., Sixl, W., Arbesser, C.H. (1988) Incidence of Q Fever among cattle, sheep and goats in the Upper Nile Province in Southern Sudan. Vet Rec, 122, 137.
  • Rolain, J.M., Gouriet, F., Brouqui, P. et al. (2005) Concomitant or consecutive infection with Coxiella burnetii and tickborne diseases. Clin Infect Dis, 40, 82-88.
  • Satta, G., Chisu, V., Cabras, P. et al. (2011) Pathogens and symbionts in ticks: A survey on tick species distribution and presence of tick-transmitted micro- organisms in Sardinia, Italy. J Med Microbiol, 60, 63-68.
  • Seyitoğlu, Ş., Özkurt, Z., Dinler, U. et al. (2006) The seroprevalence of coxiellosis in farmers and cattle in Erzurum district in Turkey. Turk J Vet Anim Sci, 30, 71- 75.
  • Spitalska, E., Kocianova, E. (2003) Detection of Coxiella burnetii in ticks collected in Slovakia and Hungary. Eur J Epidemiol, 18, 263-266.
  • Spyridaki, I., Gikas, A., Kofteridis, D., Psauroulaki, A. and Tselentis, Y. (1998) Q fever in the Greek island of Crete: Detection, isolation and molecular identification of eight strains of Coxiella burnetii from clinical samples. J Clin Microbiol, 36, 2063-2067.
  • Spyridaki, J., Psaroulaki, A., Loukaides, F. et al. (2002) Isolation of Coxiella burnetii by a centrifugation shell-vial assay from ticks collected in Cyprus: detection by nested polymerase chain reaction (PCR) and by PCR-restriction fragment length polymorphism analyses. Am J Trop Hyg, 66, 86-90.
  • Stein, A., Raoult, D. (1992) Detection of Coxiella burnetii by DNA amplification using polymerase chain reaction. J Clin Microbiol, 30, 2462-2466.
  • Zhang, G.Q., Hotta, A., Mizutani, M. et al. (1998) Direct identification of Coxiella burnetii plasmids in human sera by nested PCR. J Clin Microbiol, 36, 2210

Detection of Coxiella burnetii from ticks by Polymerase Chain Reaction and Restriction Fragment Length Polymorphism

Yıl 2013, Cilt: 60 Sayı: 4, 263 - 268, 01.12.2013
https://doi.org/10.1501/Vetfak_0000002590

Öz

For the detection of Coxiella burnetii, a total of 2472 ticks (1446 female, 1021 male and 5 nymphs) was collected from cattle and sheep of 38 provinces of Turkey. The ticks were pooled into groups of 1-7 ticks of the same province, species and gender for DNA extraction. Following DNA extraction, the groups were examined for the presence of C. burnetii DNA by using the primers CB1 and CB2. Six groups from the province of Denizli (13 groups of total 56 ticks), and one group from the province of Ankara (53 groups of total 160 ticks) were found to be positive for C. burnetii. The species of Rhipicephalus turanicus, Rhipicephalus bursa and Hyalomma excavatum were found to be infected with C. burnetii. The gender was not seem to have a role in transmission of the agent. The specificities of the PCR products were evaluated by the restriction fragment length polimorphism (RFLP) analysis. The positive PCR products were digested with the enzyme TaqІ and four bands in order of 118, 57, 43 and 39 bp’s were appeared such as seen in the positive control DNA (C. burnetii Nine Mile RSA493)

Kaynakça

  • Aitken, I.D., Bögel, K., Cracea, E. et al. (1987) Q Fever in Europe: Current aspects of aetiology, epidemiology, human infection, diagnosis and therapy. Infection, 15,323
  • Akgün, E., Yılmaz, M., Pınarbaşı, E. (2006) Q-fever şüphesi olan hasta serum ve kan örneklerinde nested-PCR yöntemiyle Coxiella burnetii’nin saptanması. Cumhuriyet Üniv Tıp Fak Derg, 28, 50-54.
  • Bernasconi, M.V., Casati, S., Peter, O., Piffaretti, J.C. (2002) Rhipicephalus ticks infected with Rickettsia and Coxiella in Southern Switzerland (Canton Ticino). Infect Genet Evol, 2,111-120.
  • Çetinkaya, B., Kalender, H., Ertaş, H.B. et al. (2000) Seroprevalence of coxiellosis in cattle, sheep and people in the east of Turkey. Vet Rec, 146, 131-136.
  • Fard, S.R.N. and Khalili, M. (2011) PCR detection of Coxiella burnetii in ticks collected from sheep and goats in southeast Iran. Iran J Arthropod-Borne Dis, 5,1-6.
  • Fournier, P.E., Marrie, T.J., Raoult, D. (1998) Diagnosis of Q-Fever. J Clin Microbiol, 36, 1823-1834.
  • Higgins, J.A., Azad, A.F. (1995) Use of polymerase chain reaction to detect bacteria in arthropods: a review. J Med Entomol, 32, 213-222.
  • Houver, A.T., Vodkin, M.H., Williams, J.C. (1992) A Coxiella burnetii repeated DNA element resembling a bacterial insertion sequence. J Bacteriol, 174, 5540-5548.
  • Inokuma, H., Yoshizaki, Y., Shimada, Y. et al. (2003) Epidemiological survey of Babesia species in Japan performed with specimens from ticks collected from dogs and detection of new Babesia DNA closely related to Babesia odocoilei and Babesia divergens DNA. J Clin Microbiol, 41, 3494-3498.
  • Kenneth, L.G., Schrumpf, M.E., Karstens, R.H. et al. (1994) DNA typing of Rickettsiae in naturally infected ticks using polymerase chain reaction/restriction fragment length polymorphism system. Am J Trop Med Hyg, 50, 247-260.
  • Kılıç, S ve Çelebi, B. (2008) Türkiye’de C. burnetii’nin epidemiyolojisi (2. Bölüm). Türk Hij Den Biyol Derg, 65 S3, 21-31.
  • Kırkan, Ş., Kaya, O., Tekbıyık, S. and Parın, U. (2008) Detection of Coxiella burnetii in cattle by PCR. Turk J Vet Anim Sci, 32, 215-220.
  • Lee, J.H., Park, H.S., Jang, W.J. et al. (2004) Identification of the Coxiella sp. detected from Haemaphysalis longicornis ticks in Korea. Microbiol Immunol, 48,125-130.
  • Masala, G., Porcu, R., Sana, G. et al. (2004) Occurence, distribution, and role in abortion of Coxiella burnetii in sheep and goats in Sardinia, Italy. Vet Microbiol, 99, 301- 302.
  • Maurin, M. and Raoult, D. (1999) Q Fever. Clin Microbiol Rev, 12, 518-553.
  • Mediannikov, O., Fenollar, F., Socolovschi, C. et al. (2010) Coxiella burnetii in Humans and ticks in rural Senegal. PLOS Negl Trop Dis, 4, e654, doi:10.1371/journal.pntd.0000654
  • Nguyen, S.V. and Hirai, K. (1999) Differentiation of Coxiella burnetii isolates by sequence determination and PCR-restriction fragment length polymorphism analysis of isocitrate dehydrogenase gene. FEMS Microbiol Lett, 180, 249-254.
  • Öngör, H., Çetinkaya, B., Karahan, M. et al. (2004) Detection of Coxiella burnetii by immunomagnetic separation-PCR in the milk of sheep in Turkey. Vet Rec, 154, 570-572.
  • Özgür, N.Y., Haköksüz, M., Yılmaz, H. et al. (1997) İnfertilite sorunu olan dişi sığırlarda ve insanlarda Coxiella burnetii antikorlarının ELISA testi ile belirlenmesi ve seroprevalansının saptanması. Pendik Vet Mikrobiyol Derg, 28, 207-217.
  • Özyer, M., Miroğlu, M., Köksal, F. (1990) Çukurova bölgesinde yaşayan insan ve hayvanlarda Q fever infeksiyonu insidansının komplement fiksasyon testi ile araştırılması. Pendik Hay Hast Merk Araşt Enst Derg, 21, 28-39.
  • Parola, P. and Raoult, D. (2001) Tick-borne bacterial diseases emerging in Europe. Clin Microbiol Infect, 7, 80- 83.
  • Psaroulaki, A., Ragiadakou, D., Kouris, G. et al. (2006) Ticks, tick-borne Rickettsiae, and Coxiella burnetii in the Greek Island of Cephalonia. Ann N Y Acad Sci, 1078, 389-399.
  • Rehacek, J. (1987) Epidemiology and significance of Q Fever in Czechoslovakia. Zentralbl Bakteriol Microbiol Hyg A, 267,16-19.
  • Rehacek, J, Urvölgyi, J., Kocianova, E. et al. (1991) Extensive examination of different tick species for infestation with Coxiella burnetii in Slovakia. Eur J Epidemiol, 7, 299-303.
  • Reinthaler, F.F., Mascher, F., Sixl, W., Arbesser, C.H. (1988) Incidence of Q Fever among cattle, sheep and goats in the Upper Nile Province in Southern Sudan. Vet Rec, 122, 137.
  • Rolain, J.M., Gouriet, F., Brouqui, P. et al. (2005) Concomitant or consecutive infection with Coxiella burnetii and tickborne diseases. Clin Infect Dis, 40, 82-88.
  • Satta, G., Chisu, V., Cabras, P. et al. (2011) Pathogens and symbionts in ticks: A survey on tick species distribution and presence of tick-transmitted micro- organisms in Sardinia, Italy. J Med Microbiol, 60, 63-68.
  • Seyitoğlu, Ş., Özkurt, Z., Dinler, U. et al. (2006) The seroprevalence of coxiellosis in farmers and cattle in Erzurum district in Turkey. Turk J Vet Anim Sci, 30, 71- 75.
  • Spitalska, E., Kocianova, E. (2003) Detection of Coxiella burnetii in ticks collected in Slovakia and Hungary. Eur J Epidemiol, 18, 263-266.
  • Spyridaki, I., Gikas, A., Kofteridis, D., Psauroulaki, A. and Tselentis, Y. (1998) Q fever in the Greek island of Crete: Detection, isolation and molecular identification of eight strains of Coxiella burnetii from clinical samples. J Clin Microbiol, 36, 2063-2067.
  • Spyridaki, J., Psaroulaki, A., Loukaides, F. et al. (2002) Isolation of Coxiella burnetii by a centrifugation shell-vial assay from ticks collected in Cyprus: detection by nested polymerase chain reaction (PCR) and by PCR-restriction fragment length polymorphism analyses. Am J Trop Hyg, 66, 86-90.
  • Stein, A., Raoult, D. (1992) Detection of Coxiella burnetii by DNA amplification using polymerase chain reaction. J Clin Microbiol, 30, 2462-2466.
  • Zhang, G.Q., Hotta, A., Mizutani, M. et al. (1998) Direct identification of Coxiella burnetii plasmids in human sera by nested PCR. J Clin Microbiol, 36, 2210
Toplam 33 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Veteriner Cerrahi
Diğer ID JA59YJ79GE
Bölüm Araştırma Makalesi
Yazarlar

Çapin Gülay Altay

Zişan Emre

Seyit Canpolat

Yusuf Vatansever

Ali Düzgün

Yayımlanma Tarihi 1 Aralık 2013
Yayımlandığı Sayı Yıl 2013Cilt: 60 Sayı: 4

Kaynak Göster

APA Altay, Ç. G., Emre, Z., Canpolat, S., Vatansever, Y., vd. (2013). Detection of Coxiella burnetii from ticks by Polymerase Chain Reaction and Restriction Fragment Length Polymorphism. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 60(4), 263-268. https://doi.org/10.1501/Vetfak_0000002590
AMA Altay ÇG, Emre Z, Canpolat S, Vatansever Y, Düzgün A. Detection of Coxiella burnetii from ticks by Polymerase Chain Reaction and Restriction Fragment Length Polymorphism. Ankara Univ Vet Fak Derg. Aralık 2013;60(4):263-268. doi:10.1501/Vetfak_0000002590
Chicago Altay, Çapin Gülay, Zişan Emre, Seyit Canpolat, Yusuf Vatansever, ve Ali Düzgün. “Detection of Coxiella Burnetii from Ticks by Polymerase Chain Reaction and Restriction Fragment Length Polymorphism”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 60, sy. 4 (Aralık 2013): 263-68. https://doi.org/10.1501/Vetfak_0000002590.
EndNote Altay ÇG, Emre Z, Canpolat S, Vatansever Y, Düzgün A (01 Aralık 2013) Detection of Coxiella burnetii from ticks by Polymerase Chain Reaction and Restriction Fragment Length Polymorphism. Ankara Üniversitesi Veteriner Fakültesi Dergisi 60 4 263–268.
IEEE Ç. G. Altay, Z. Emre, S. Canpolat, Y. Vatansever, ve A. Düzgün, “Detection of Coxiella burnetii from ticks by Polymerase Chain Reaction and Restriction Fragment Length Polymorphism”, Ankara Univ Vet Fak Derg, c. 60, sy. 4, ss. 263–268, 2013, doi: 10.1501/Vetfak_0000002590.
ISNAD Altay, Çapin Gülay vd. “Detection of Coxiella Burnetii from Ticks by Polymerase Chain Reaction and Restriction Fragment Length Polymorphism”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 60/4 (Aralık 2013), 263-268. https://doi.org/10.1501/Vetfak_0000002590.
JAMA Altay ÇG, Emre Z, Canpolat S, Vatansever Y, Düzgün A. Detection of Coxiella burnetii from ticks by Polymerase Chain Reaction and Restriction Fragment Length Polymorphism. Ankara Univ Vet Fak Derg. 2013;60:263–268.
MLA Altay, Çapin Gülay vd. “Detection of Coxiella Burnetii from Ticks by Polymerase Chain Reaction and Restriction Fragment Length Polymorphism”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, c. 60, sy. 4, 2013, ss. 263-8, doi:10.1501/Vetfak_0000002590.
Vancouver Altay ÇG, Emre Z, Canpolat S, Vatansever Y, Düzgün A. Detection of Coxiella burnetii from ticks by Polymerase Chain Reaction and Restriction Fragment Length Polymorphism. Ankara Univ Vet Fak Derg. 2013;60(4):263-8.