Ankara Üniversitesi Veteriner Fakültesi Dergisi
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Evaluation of the effect of platelet rich plasma (PRP) on enhancement of bone healing in diaphyseal bone defects by radiography and computed tomography

Yıl 2010, Cilt: 57 Sayı: 2, 77 - 82, 01.06.2010

Ahmet ÖZAK Cenk YARDIMCI Özlem H. NİSBET İlkay Koray BAYRAK Cevat NİSBET

https://doi.org/10.1501/Vetfak_0000002314

Öz

The effect of platelet-rich plasma (PRP) with autogenous cancellous bone graft on enhancement of bone healing in diaphyseal bone defects was evaluated. A 4-mm defect was created in the middiaphysis of the tibias of 20 rabbits. Rabbits were divided into two groups of ten animals each: only autogenous cancellous graft, PRP and autogenous cancellous graft. In animals of group 1, only autogenous cancellous grafts, and to those in group 2, PRP and autogenous cancellous grafts, were applied to the defect. Radiographical and computed tomography (CT) views were taken and evaluated on postoperative days 0, 15, 30, 60, and 90. According to the bone formation, union, and remodeling scores, group 1 had better scores than group 2 on days 30, 60, and 90. The density was significantly increased on day 60 than on days 0, 15, and 30 in group 1. In conclusion, it was evaluated that PRP could not enhance the bone regeneration in diaphyseal defects when used with autogenous cancellous bone graft

Anahtar Kelimeler

Bone regeneration, computed tomography (CT), platelet rich plasma (PRP), radiography

Kaynakça

  • Aghaloo TL, Moy PK, Freymiller EG (2004): Evaluation of platelet-rich plasma in combination with anorganic bovine bone in the rabbit cranium: A pilot study. Int J Oral Maxillofac Implants, 19, 59–65.
  • Akca K, Cehreli M, Demiralp B, Guzel E, Dagdeviren A (2007): Platelet-rich plasma and bone healing: A histologic study in titanium bone chambers. Int J Periodontics Restorative Dent, 27, 387–392.
  • Borzini P, Mazzucco L (2007): Platelet- Rich plasma (PRP) and platelet derivatives for topical therapy. What is true from the biological wiev point? ISBT Sci Ser, 2, 272- 281.
  • Boudrieau RJ (2002): Fractures of the tibia and fibula. 2144. In: Slatter D. (Ed), Textbook of Small Animal Surgery. Saunders Co., New York.
  • Choi BH, Im CJ, Huh JY, Suh JJ, Lee SH (2004): Effect of platelet-rich plasma on bone regeneration in autogenous bone graft. Int J Oral Maxillofac Surg, 33, 56– 59.
  • Dallari D, Fini M, Stagni C, Torricelli P, Nicoli Aldini N, Giavaresi G, Cenni E, Baldini N, Cenacchi A, Bassi A, Giardino R, Fornasari PM, Giunti (2006): In vivo study on the healing of bone defects treated with bone marrow stromal cells, platelet-rich plasma, and freeze- dried bone allografts, alone and in combination. J Orthop Res, 24, 877-888.
  • Dugrillon A, Klüter H (2002): Topical application of platelets for improved wound healing. Blood Ther Med, 3, 21–26.
  • Hatakeyama M, Beletti ME, Zanetta-Barbosa D, Dechichi P (2008): Radiographic and histomorphometric analysis of bone healing using autogenous graft associated with platelet-rich plasma obtained by 2 different methods. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 105, 13-18.
  • John PWM (1971): Statistical Design and Analysis of Experiments. Siam, Philadelphia.
  • Kasten P, Vogel J, Geiger F, Niemeyer P, Luginbühl R, Szalay K (2008): The effect of platelet-rich plasma on healing in critical-size long-bone defects. Biomaterials, 29, 3983–3992.
  • Katz MH, Alvarez AF, Kirsner RS, Eaglstein WH, Falanga V (1991): Human wound fluid from acute wounds stimulates fibroblast and endothelial cell growth. J Am Acad Dermatol, 25, 1054-1062.
  • Lane JM, Sandhu HS (1987): Current approaches to experimental bone grafting. Orthop Clin North Am, 18, 213-225.
  • Marcellin-Little DJ (2002): External skeletal fixation. 1820-1826. In: Slatter D. (Ed), Textbook of Small Animal Surgery. Saunders Co., New York.
  • Marx RE, Carlson ER, Eichstaedt RM, Schimmele SR, Strauss JE, Georgeff KR (1998): Platelet-rich plasma: Growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 85, 638–646.
  • Marx RE (2001): The biology of platelet-rich plasma. J Oral Maxillofac Surg, 59, 1119–1121.
  • Mehta S, Watson JT (2008): Platelet rich concentrate: Basic science and current clinical applications. J Orthop Trauma, 22, 432-438.
  • Messora MR, Nagata MJ, Dornelles RC, Bomfim SR, Furlaneto FA, de Melo LG, Deliberador TM, Bosco AF, Garcia VG, Fucini SE (2008): Bone healing in critical- size defects treated with platelet-rich plasma activated by two different methods. A histologic and histometric study in rat calvaria. J Periodontal Res, 43, 723-729.
  • Mooren RE, Merkx MA, Bronkhorst EM, Jansen JA, Stoelinga PJ (2007): The effect of platelet-rich plasma on early and late bone healing: An experimental study in goats. Int J Oral Maxillofac Surg, 36, 626-631.
  • Rai B, Oest ME, Dupont KM, Ho KH, Teoh SH, Guldberg RE (2007): Combination of platelet-rich plasma with polycaprolactone-tricalcium phosphate scaffolds for segmental bone defect repair. J Biomed Mater Res A, 81, 888-899.
  • Sarkar MR, Augat P, Shefelbine SJ, Schorlemmer S, Huber-Lang M, Claes L, Kinzl L, Ignatius A (2006): Bone formation in a long bone defect model using a platelet-rich plasma-loaded collagen scaffold. Biomaterials, 27, 1817-1823.
  • Thibault L, Beausejour A, de Grandmont MJ, Lemieux R, Leblanc JF (2006) : Characterization of blood components prepared from whole-blood donations after a 24-hour hold with the platelet-rich plasma method. Transfusion, 46, 1292-1299.
  • Wiltfang J, Kloss FR, Kessler P, Nkenke E, Schultze- Mosgau S, Zimmermann R, Schlegel KA (2004): Effects of platelet-rich plasma on bone healing in combination with autogenous bone and bone substitutes in critical-size defects. Clin Oral Implants Res, 15, 187–193.

Diyafizeal kemik defektlerinde trombositten zengin plazmanın (PRP) kemik iyileşmesini artırmaüzerindeki etkisinin radyografi ve bilgisayarlı tomografi ile değerlendirilmesi

Yıl 2010, Cilt: 57 Sayı: 2, 77 - 82, 01.06.2010

Ahmet ÖZAK Cenk YARDIMCI Özlem H. NİSBET İlkay Koray BAYRAK Cevat NİSBET

https://doi.org/10.1501/Vetfak_0000002314

Öz

Bu çalışmada; trombositten zengin plazmanın (PRP), otojen kansellöz greftler ile birlikte diyafizer defektlerde kemik rejenerasyonuna olan etkisi değerlendirildi. Yirmi tavşanda tibianın orta 1/3 diyafizinde 4 mm. genişliğinde defekt oluşturuldu. Tavşanlar, her birinde 10 tavşan içeren iki gruba ayrıldı, birinci gruptaki tavşanlara sadece otojenöz kansellöz greft, ikinci gruptakilere PRP ile birlikte otojenöz kansellöz greft yerleştirildi. Operasyon sonrası 0., 15., 30., 60. ve 90. günlerde radyografi ve bilgisayarlı tomografi (BT) çekimleri yapıldı ve değerlendirildi. Kemik oluşumu, kaynama ve yeniden şekillenme derecelendirmesine göre 30., 60. ve 90. günlerde birinci grupta, ikinci gruba göre daha iyi sonuçlar elde edildi. Birinci grupta dansite, 60. günde 0., 15. ve 30. güne göre daha belirgin bir artış gösterdi. Sonuç olarak; PRP, otojenöz kansellöz greftler ile birlikte diyafizer kemik defektlerine uygulandığında, kemik rejenerasyonunu artırıcı hiçbir katkısı olmadığı anlaşıldı

Anahtar Kelimeler

Bilgisayarlı tomografi (BT), kemik rejenerasyonu, radyografi trombositten zengin plazma (PRP)

Kaynakça

  • Aghaloo TL, Moy PK, Freymiller EG (2004): Evaluation of platelet-rich plasma in combination with anorganic bovine bone in the rabbit cranium: A pilot study. Int J Oral Maxillofac Implants, 19, 59–65.
  • Akca K, Cehreli M, Demiralp B, Guzel E, Dagdeviren A (2007): Platelet-rich plasma and bone healing: A histologic study in titanium bone chambers. Int J Periodontics Restorative Dent, 27, 387–392.
  • Borzini P, Mazzucco L (2007): Platelet- Rich plasma (PRP) and platelet derivatives for topical therapy. What is true from the biological wiev point? ISBT Sci Ser, 2, 272- 281.
  • Boudrieau RJ (2002): Fractures of the tibia and fibula. 2144. In: Slatter D. (Ed), Textbook of Small Animal Surgery. Saunders Co., New York.
  • Choi BH, Im CJ, Huh JY, Suh JJ, Lee SH (2004): Effect of platelet-rich plasma on bone regeneration in autogenous bone graft. Int J Oral Maxillofac Surg, 33, 56– 59.
  • Dallari D, Fini M, Stagni C, Torricelli P, Nicoli Aldini N, Giavaresi G, Cenni E, Baldini N, Cenacchi A, Bassi A, Giardino R, Fornasari PM, Giunti (2006): In vivo study on the healing of bone defects treated with bone marrow stromal cells, platelet-rich plasma, and freeze- dried bone allografts, alone and in combination. J Orthop Res, 24, 877-888.
  • Dugrillon A, Klüter H (2002): Topical application of platelets for improved wound healing. Blood Ther Med, 3, 21–26.
  • Hatakeyama M, Beletti ME, Zanetta-Barbosa D, Dechichi P (2008): Radiographic and histomorphometric analysis of bone healing using autogenous graft associated with platelet-rich plasma obtained by 2 different methods. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 105, 13-18.
  • John PWM (1971): Statistical Design and Analysis of Experiments. Siam, Philadelphia.
  • Kasten P, Vogel J, Geiger F, Niemeyer P, Luginbühl R, Szalay K (2008): The effect of platelet-rich plasma on healing in critical-size long-bone defects. Biomaterials, 29, 3983–3992.
  • Katz MH, Alvarez AF, Kirsner RS, Eaglstein WH, Falanga V (1991): Human wound fluid from acute wounds stimulates fibroblast and endothelial cell growth. J Am Acad Dermatol, 25, 1054-1062.
  • Lane JM, Sandhu HS (1987): Current approaches to experimental bone grafting. Orthop Clin North Am, 18, 213-225.
  • Marcellin-Little DJ (2002): External skeletal fixation. 1820-1826. In: Slatter D. (Ed), Textbook of Small Animal Surgery. Saunders Co., New York.
  • Marx RE, Carlson ER, Eichstaedt RM, Schimmele SR, Strauss JE, Georgeff KR (1998): Platelet-rich plasma: Growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 85, 638–646.
  • Marx RE (2001): The biology of platelet-rich plasma. J Oral Maxillofac Surg, 59, 1119–1121.
  • Mehta S, Watson JT (2008): Platelet rich concentrate: Basic science and current clinical applications. J Orthop Trauma, 22, 432-438.
  • Messora MR, Nagata MJ, Dornelles RC, Bomfim SR, Furlaneto FA, de Melo LG, Deliberador TM, Bosco AF, Garcia VG, Fucini SE (2008): Bone healing in critical- size defects treated with platelet-rich plasma activated by two different methods. A histologic and histometric study in rat calvaria. J Periodontal Res, 43, 723-729.
  • Mooren RE, Merkx MA, Bronkhorst EM, Jansen JA, Stoelinga PJ (2007): The effect of platelet-rich plasma on early and late bone healing: An experimental study in goats. Int J Oral Maxillofac Surg, 36, 626-631.
  • Rai B, Oest ME, Dupont KM, Ho KH, Teoh SH, Guldberg RE (2007): Combination of platelet-rich plasma with polycaprolactone-tricalcium phosphate scaffolds for segmental bone defect repair. J Biomed Mater Res A, 81, 888-899.
  • Sarkar MR, Augat P, Shefelbine SJ, Schorlemmer S, Huber-Lang M, Claes L, Kinzl L, Ignatius A (2006): Bone formation in a long bone defect model using a platelet-rich plasma-loaded collagen scaffold. Biomaterials, 27, 1817-1823.
  • Thibault L, Beausejour A, de Grandmont MJ, Lemieux R, Leblanc JF (2006) : Characterization of blood components prepared from whole-blood donations after a 24-hour hold with the platelet-rich plasma method. Transfusion, 46, 1292-1299.
  • Wiltfang J, Kloss FR, Kessler P, Nkenke E, Schultze- Mosgau S, Zimmermann R, Schlegel KA (2004): Effects of platelet-rich plasma on bone healing in combination with autogenous bone and bone substitutes in critical-size defects. Clin Oral Implants Res, 15, 187–193.

Ayrıntılar

Birincil Dil İngilizce
Konular Veteriner Hekimlik
Diğer ID JA87RT67ER
Bölüm Araştırma Makalesi
Yazarlar

Ahmet ÖZAK
Department of Surgery, Faculty of Veterinary Medicine, Ondokuz Mayıs University

Cenk YARDIMCI
Department of Surgery, Faculty of Veterinary Medicine, Ondokuz Mayıs University

Özlem H. NİSBET
Department of Surgery, Faculty of Veterinary Medicine, Ondokuz Mayıs University

İlkay Koray BAYRAK
Department of Radiology, Faculty of Medicine, Ondokuz Mayıs University

Cevat NİSBET
Department of Biochemistry, Faculty of Veterinary Medicine, Ondokuz Mayıs University

Yayımlanma Tarihi 1 Haziran 2010
Yayınlandığı Sayı Yıl 2010Cilt: 57 Sayı: 2

Kaynak Göster

Bibtex @editöre mektup { auvfd556049, journal = {Ankara Üniversitesi Veteriner Fakültesi Dergisi}, issn = {1300-0861}, eissn = {1308-2817}, address = {}, publisher = {Ankara Üniversitesi}, year = {2010}, volume = {57}, number = {2}, pages = {77 - 82}, doi = {10.1501/Vetfak\_0000002314}, title = {Evaluation of the effect of platelet rich plasma (PRP) on enhancement of bone healing in diaphyseal bone defects by radiography and computed tomography}, key = {cite}, author = {Özak, Ahmet and Yardımcı, Cenk and Nisbet, Özlem H. and Bayrak, İlkay Koray and Nisbet, Cevat} }
APA Özak, A. , Yardımcı, C. , Nisbet, Ö. H. , Bayrak, İ. K. & Nisbet, C. (2010). Evaluation of the effect of platelet rich plasma (PRP) on enhancement of bone healing in diaphyseal bone defects by radiography and computed tomography . Ankara Üniversitesi Veteriner Fakültesi Dergisi , 57 (2) , 77-82 . DOI: 10.1501/Vetfak_0000002314
MLA Özak, A. , Yardımcı, C. , Nisbet, Ö. H. , Bayrak, İ. K. , Nisbet, C. "Evaluation of the effect of platelet rich plasma (PRP) on enhancement of bone healing in diaphyseal bone defects by radiography and computed tomography" . Ankara Üniversitesi Veteriner Fakültesi Dergisi 57 (2010 ): 77-82 <http://vetjournal.ankara.edu.tr/tr/pub/issue/44740/556049>
Chicago Özak, A. , Yardımcı, C. , Nisbet, Ö. H. , Bayrak, İ. K. , Nisbet, C. "Evaluation of the effect of platelet rich plasma (PRP) on enhancement of bone healing in diaphyseal bone defects by radiography and computed tomography". Ankara Üniversitesi Veteriner Fakültesi Dergisi 57 (2010 ): 77-82
RIS TY - JOUR T1 - Diyafizeal kemik defektlerinde trombositten zengin plazmanın (PRP) kemik iyileşmesini artırmaüzerindeki etkisinin radyografi ve bilgisayarlı tomografi ile değerlendirilmesi AU - AhmetÖzak, CenkYardımcı, Özlem H.Nisbet, İlkay KorayBayrak, CevatNisbet Y1 - 2010 PY - 2010 N1 - doi: 10.1501/Vetfak_0000002314 DO - 10.1501/Vetfak_0000002314 T2 - Ankara Üniversitesi Veteriner Fakültesi Dergisi JF - Journal JO - JOR SP - 77 EP - 82 VL - 57 IS - 2 SN - 1300-0861-1308-2817 M3 - doi: 10.1501/Vetfak_0000002314 UR - https://doi.org/10.1501/Vetfak_0000002314 Y2 - 2023 ER -
EndNote %0 Ankara Üniversitesi Veteriner Fakültesi Dergisi Evaluation of the effect of platelet rich plasma (PRP) on enhancement of bone healing in diaphyseal bone defects by radiography and computed tomography %A Ahmet Özak , Cenk Yardımcı , Özlem H. Nisbet , İlkay Koray Bayrak , Cevat Nisbet %T Evaluation of the effect of platelet rich plasma (PRP) on enhancement of bone healing in diaphyseal bone defects by radiography and computed tomography %D 2010 %J Ankara Üniversitesi Veteriner Fakültesi Dergisi %P 1300-0861-1308-2817 %V 57 %N 2 %R doi: 10.1501/Vetfak_0000002314 %U 10.1501/Vetfak_0000002314
ISNAD Özak, Ahmet , Yardımcı, Cenk , Nisbet, Özlem H. , Bayrak, İlkay Koray , Nisbet, Cevat . "Evaluation of the effect of platelet rich plasma (PRP) on enhancement of bone healing in diaphyseal bone defects by radiography and computed tomography". Ankara Üniversitesi Veteriner Fakültesi Dergisi 57 / 2 (Haziran 2010): 77-82 . https://doi.org/10.1501/Vetfak_0000002314
AMA Özak A. , Yardımcı C. , Nisbet Ö. H. , Bayrak İ. K. , Nisbet C. Evaluation of the effect of platelet rich plasma (PRP) on enhancement of bone healing in diaphyseal bone defects by radiography and computed tomography. Ankara Univ Vet Fak Derg. 2010; 57(2): 77-82.
Vancouver Özak A. , Yardımcı C. , Nisbet Ö. H. , Bayrak İ. K. , Nisbet C. Evaluation of the effect of platelet rich plasma (PRP) on enhancement of bone healing in diaphyseal bone defects by radiography and computed tomography. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2010; 57(2): 77-82.
IEEE A. Özak , C. Yardımcı , Ö. H. Nisbet , İ. K. Bayrak ve C. Nisbet , "Evaluation of the effect of platelet rich plasma (PRP) on enhancement of bone healing in diaphyseal bone defects by radiography and computed tomography", Ankara Üniversitesi Veteriner Fakültesi Dergisi, c. 57, sayı. 2, ss. 77-82, Haz. 2010, doi:10.1501/Vetfak_0000002314