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The effect of vitamin E on lipid peroxidation and some antioxidants in blood and milk of cows with subclinical mastitis

Yıl 2005, Cilt: 52 Sayı: 2, 71 - 76, 01.06.2005

Halil Şimşek Mesut Aksakal

https://doi.org/10.1501/Vetfak_0000000039

Öz

This study was carried out to determine the effects of vitamin E on lipid peroxidation and some antioxidant substances in subclinical mastitic dairy cows. Forty cows were assigned to two groups by California Mastitis Test (CMT) and somatic cell count (SCC). 2.000 IU vitamin E was injected to cows of mastitic group by intramuscular route every other day for twenty days. Blood and milk samples from cows in healthy, mastitic and treatment groups were taken; milk SCC was counted vitamin E, Se and MDA concentrations in milk and plasma, erytrocyte GSH, GSH-Px and Catalase activities and leucocyte GSH-Px and MDA levels were determined. Differences in milk SCC, vitamin E and MDA, plasma vitamin E, Se and MDA; leucocyte GSH-Px; erytrocyte GSH, GSH-Px and Catalase levels between healthy and mastitic groups were significantly changed while differences in milk Se and leucocyte MDA levels were not significant. Mastitic and treatment groups in milk SCC and MDA, plasma vitamin E and MDA; leucocyte GSH-Px; erythrocyte GSH and GSH-Px levels were found as significant stastistically whereas differences in milk vitamin E and Se levels; plasma Se; leucocyte MDA and erythrocyte Catalase activities were not stastistically significant. Differences in milk SCC and MDA, plasma Se and MDA; erytrocyte GSH and GSH-Px; leucocyte GSH-Px levels between healthy and treatment groups were significantly changed while differences in milk vitamin E and Se, plasma vitamin E, erythrocyte Catalase activities and leucocyte MDA levels were not significant

Anahtar Kelimeler

Antioxidants lipid peroxidation selenium subclinical mastitis vitamin E

Kaynakça

  • Aksakal M, Çay M, Nazıroğlu M (1997): Ratlarda E vitamininin alveolar ve peritonal makrofajların fagositik aktivitesi üzerindeki etkisi. Fırat Üniv Sağ Bil Derg, 11, 183-189.
  • Anne Marie STL, Hıdıroglu M, Snoddon M, Nicholson JWG (1990): Effect of α-tocopherol supplementation to dairy cows on milk and plasma α-tocopherol concentrations and on spontaneous oxidazed flavor in milk. Can J Anim Sci, 70, 561-570.
  • Atroshi F, Parantainen J, Sankari S, Osterman T (1986): Prostaglandins and glutathione proxidase in bovine mastitis. Res Vet Sci, 40, 361-366.
  • Atroshi F, Sankari S, Rizzo A, Westermarck T, Parantainen J (1990): Prostoglandins, Glutathione Metabolism and Lipid Peroxidation in Relation to Inflammation in Bovine Mastitis. Antioxidants in Therapy and Preventive Medicine Plenum Press, New York.
  • Atroshi F, Työppönen J, Sankari S, Kangasniemi R, Parantainen J (1986): Possible roles of vitamin E and glutathione metabolism in bovine mastitis. Internat J Vit Nutr Res, 57, 37-43.
  • Aydın F, Leloğlu N, Çolak A, Otlu S (1995): Kars yöresi süt ineklerinde klinik ve subklinik mastitislere neden olan mikroorganizmaların identifikasyonları ve antibiyotiklere duyarlılıkları üzerine araştırmalar. Pendik Vet Mikrob Derg, 26, 55-65.
  • Aydın N (1989): T.C. Tarım Orman ve Köyişleri Bakanlı- ğı, Koruma ve Kontrol Genel Müdürlüğü. Mastitis Projesi. MPDH-HH-113 T No: 22. ANKARA.
  • Aziz ES, Klesius PH, Frandsen JC (1984): Effects of selenium on polymorphonuclear leukocyte function in goats. Am J Vet Res, 45, 1715-1718.
  • Babior BM (1984): The respiratory burst of phagocytes. J Clin Invest, 8, 599-601.
  • Batra TR, Singh K, Ho SK, Hıdıroglu M (1991): Concentration of plasma and milk vitamin E and plasma β- carotene of mastitic and healthy cows. J Vit Nutr Res, 62, 233-237.
  • Braun U, Forrer WF, Lutz H (1991): Selenium and vitamin E in blood sera of cows from farms with increased incidence of disease. Vet Rec, 128, 543-547.
  • Colitti M, Stradaidi G, Stefanon B (2000): Effect of α- tocopherol deprivation on the involution of mammary gland in sheep. J Dairy Sci, 83, 345-350.
  • Combs GF, Combs BS (1986): The Role of Selenium Nutrition. Academic Press. Inc London.
  • Craven N, Williams MR (1985): Defense of bovine mammary gland against infection and prospect for their enhancements. Vet Immunol Immunopat, 2, 71-78.
  • Çay M (1996): Ratlarda Selenyum ve Vitamin E’nin Alveolar ve Peritoneal Makrofajların Fagositik Aktivitesi Üzerindeki Etkisi. Fırat Üniversitesi Sağlık Bilimleri Ensti- tüsü Doktora Tezi, Elazığ.
  • Dowel LRM (1989): Vitamins in Animal Nutrition Comparative Aspects to Human Nutrition. Vitamin E. Academic Press, London
  • Dündar Y, Eryavuz A, Aslan R, Uçar M (2000): Malandialdehyde and glucose-6-phosphate dehydrogenase levels in healthy and subclinical mastitic cows, Yüzüncü Yıl Üniv Sağ Bil Derg, 6, 84-86.
  • Ergun H, Mert N (1984): Sütte mastitis nedeniyle meyda- na gelen biyokimyasal değişmeler. 1 Mastitis Semineri 24- Kasım Ankara Üniversitesi Veteriner Fakültesi Ankara.
  • Gornal AG, Bardawill CJ, David MM (1975): Determination of serum proteins by means of the biüret reaction. J Biol Chem, 177: 751.
  • Goth L (1991): A simple method for determination of serum catalase activity and revision of reference range. Clin Chim Acta, 196, 143-152.
  • Hogan JS, Smith KL, Weiss WP, Todhunter DA, Schockey WL (1990): Relationships among vitamin E, selenium and bovine blood neutrophils. J Dairy Sci, 73, 2372-2378.
  • Hogan JS, Weiss WP, Smith K, Soridillo LM, Williams SN (1996): α-tocopherol concentrations in milk and plasma during clinical Escherichia coli mastitis. J Dairy Sci, 79, 71-75.
  • Jain NC (1979): Common mammary pathogens and factors in infection and mastitis. J Dairy Sci, 62, 128-134.
  • Johnston LA, Chew BP (1984): Peripartum of plasma and milk vitamin A and β-carotene among dairy cows with or without mastitis. J Dairy Sci, 67, 1832-1840.
  • Kayden HJ, Chow CK, Bjarnson LK. (1973): Spectrophotometric method for determination of tocopherol in red blood cells. J Lip Res, 14, 533-540.
  • Lawrence RA, Burk RF (1976): Glutathione peroxidase activity in selenium-deficient rat liver. Bioch Bioph Res Commun, 71, 952-958.
  • Mates JM, Sanchez-Jimenes F (1999): Antioxidant enzymes and their implications in pathophsiologic processes. Front Biosci, 15, 339-345.
  • Matkovics B, Szabo I, Varga IS. (1988): Determination of enzyme activities in lipid peroxidation and glutathionep pathways (in Hungarian). Lab Diag, 15, 248-249.
  • Mayer SJ, Wterman AE, Keen PM, Craven N (1988): Oxygen concentration in milk of healthy and mastitic cows and implications of oxygen tension. J Dairy Sci, 55, 513- 519.
  • Morgante M, Beghelli D, Pausellim M, Dallara P, Capucella M, Ranucci S (1999): Effect of administration of vitamin E and selenium during the dary period on mammaray health and milk cell counts in dairy ewes. J Dairy Sci, 82, 623-631.
  • Ndiweni N, Field TR, Williams MR, Booth JM, Finc JM (1991): Studies on the incidence of clinical mastitis and blood levels of vitamin E and selenium in dairy herds in England. Vet Rec, 129, 86-88.
  • Nizamlıoğlu M, Dinç DA, Erganiş O, Özeren F, Uçan S. (1992): Subklinik mastitisli koyunlarda N- asetil B-D glikozaminidaz (NAG ase) enzimi ve bazı biyokimyasal de- ğerlerin araştırılması.Veterinarium, 3, 12-16.
  • Placer ZA, Cushmann LL, Johnson BC (1966): Estimation of products of lipid peroxidation in biochhemical systems. Anal Biochem, 16, 359-364.
  • Sandholm M, Mattila T (1986): Biochemical aspects of bovine mastitis. Isr J Vet Med, 42, 405-415.
  • Schalm OW, Carrol EJ, Jain NC (1971): Bovine Mastitis. First Ed-Lea-Febriger London.
  • Scholz RW, Hutchinson LJ (1979): Distribution of glutathione peroxidase activity and selenium in the blood of dairy cows. Am J Vet Res, 40, 245-249.
  • Sedlak J, Lindsay RHC (1968): Estimation of total prote- in bound and nonprotein sulfhydryl groups in tissue with Ellmann’s reagent. Anal Biochem, 25, 192-205.
  • Serfass RE, Ganther HE (1976): Effects of dietary selenium and tocopherol on glutathione peroxidase and superoxide dismutase activities in Rat. Phago-Life Sci, 19, 1139-1144.
  • Smith KL (1983): Mastitis control: A discussion. J Dairy Sci, 66, 1790-1794.
  • Smith KL, Conrad HR, Amiet BA, Todhunter DA (1985): Incidence of enviromental mastitis as influenced by dietary vitamin E and selenium. Kieler Milch Forsc Benic, 37, 482-486.
  • Stocchini A, Coni E, Baldini M, Beccalno E, Caroli S (1989): Selenium intake with diet in Italy. A plat study. J Trace Elem Electrol Health Dis, 3, 193-198.
  • Stratton SP, Liebler DC (1997): Determination of siglet oxygen-specific versus radical-mediated lipid peroxidation in photosensitized oxidation of lipid bilayers; effect of β- carotene and α-tocopherol. Biochemistry, 36, 12911- 12920.
  • Sümbüloğlu K, Sümbüloğlu V (1995): Biyoistatistik. Özdemir Basım Yayım ve Dağıtım LTD Şti 6. Baskı, An- kara.
  • Wuryastuti H, Stowe HD, Bull RW, Miller ER (1993): Effects of vitamin E and seleniım on immune responses of peripheral blood, colostrum and milk leukocytes of sows. J Anim Sci, 71, 2464-2472.
  • Yarım G, Salmanoğlu B (2002): Subklinik mastitisli süt ineklerinde meme içi levamizol uygulanmasında süt ve kanda glutatyon peroksidaz, süperoksit dismutaz, alkalen fosfotaz ve immunoglobulin G düzeyleri. Ankara Üniv Vet Fak Derg, 49, 89-94.

Subklinik mastitisli ineklerde kan ve sütte lipit peroksidasyon ve bazı antioksidanlar üzerine E vitamininin etkisi

Yıl 2005, Cilt: 52 Sayı: 2, 71 - 76, 01.06.2005

Halil Şimşek Mesut Aksakal

https://doi.org/10.1501/Vetfak_0000000039

Öz

Bu araştırmada, subklinik mastitisli ineklerde E vitamininin lipit peroksidasyon ve bazı antioksidan düzeylerine etkisi araştırıldı. Araştırmada, 40 adet inek kullanıldı. Sağlıklı ve mastitisli inekler, Kaliforniya Mastitis Testi (CMT) ve somatik hücre sayısına (SHS) göre belirlendi. Mastitisli ineklere 20 gün boyunca gün aşırı 2.000 IU E vitamini kas içi uygulandı. Sağlıklı, mastitisli ve tedavi gruplarındaki ineklerden alınan kan ve süt örneklerinde süt SHS, süt ve plazma E vitamini, Se ve MDA, eritrosit GSH, GSH-Px ve Katalaz, lökosit GSH-Px ve MDA değerlerine bakıldı. Sağlıklı ve mastitisli grup süt SHS, E vitamini ve MDA, plazma E vitamini, Se ve MDA, lökosit GSH-Px, eritrosit GSH, GSH-Px ve Katalaz düzeyleri arasındaki fark istatistiksel olarak önemli bulundu. Süt Se ve lökosit MDA ortalamaları ise önemsiz bulundu. Mastitisli ve tedavi grupları arasında süt SHS ve MDA, plazma E vitamini ve MDA, lökosit GSH-Px, eritrosit GSH ve GSH-Px düzeyleri arasındaki fark istatistiksel olarak önemli bulundu. Süt E vitamini ve Se, plazma Se, lökosit MDA ve eritrosit Katalaz ortalamaları farkının ise istatistiksel yönden önemsiz olduğu saptandı. Sağlıklı ve tedavi grupları arasında süt SHS ve MDA, plazma Se ve MDA, eritrosit GSH ve GSH-Px, lökosit GSH-Px, düzeyleri arasındaki fark istatistiksel olarak önemli bulundu. Süt E vitamini ve Se, plazma E vitamini, eritrosit Katalaz, lökosit MDA ortalamaları farkının ise istatistiksel yönden önemsiz olduğu saptandı

Anahtar Kelimeler

Antioksidanlar E vitamini lipit peroksidasyon selenyum subklinik mastitis

Kaynakça

  • Aksakal M, Çay M, Nazıroğlu M (1997): Ratlarda E vitamininin alveolar ve peritonal makrofajların fagositik aktivitesi üzerindeki etkisi. Fırat Üniv Sağ Bil Derg, 11, 183-189.
  • Anne Marie STL, Hıdıroglu M, Snoddon M, Nicholson JWG (1990): Effect of α-tocopherol supplementation to dairy cows on milk and plasma α-tocopherol concentrations and on spontaneous oxidazed flavor in milk. Can J Anim Sci, 70, 561-570.
  • Atroshi F, Parantainen J, Sankari S, Osterman T (1986): Prostaglandins and glutathione proxidase in bovine mastitis. Res Vet Sci, 40, 361-366.
  • Atroshi F, Sankari S, Rizzo A, Westermarck T, Parantainen J (1990): Prostoglandins, Glutathione Metabolism and Lipid Peroxidation in Relation to Inflammation in Bovine Mastitis. Antioxidants in Therapy and Preventive Medicine Plenum Press, New York.
  • Atroshi F, Työppönen J, Sankari S, Kangasniemi R, Parantainen J (1986): Possible roles of vitamin E and glutathione metabolism in bovine mastitis. Internat J Vit Nutr Res, 57, 37-43.
  • Aydın F, Leloğlu N, Çolak A, Otlu S (1995): Kars yöresi süt ineklerinde klinik ve subklinik mastitislere neden olan mikroorganizmaların identifikasyonları ve antibiyotiklere duyarlılıkları üzerine araştırmalar. Pendik Vet Mikrob Derg, 26, 55-65.
  • Aydın N (1989): T.C. Tarım Orman ve Köyişleri Bakanlı- ğı, Koruma ve Kontrol Genel Müdürlüğü. Mastitis Projesi. MPDH-HH-113 T No: 22. ANKARA.
  • Aziz ES, Klesius PH, Frandsen JC (1984): Effects of selenium on polymorphonuclear leukocyte function in goats. Am J Vet Res, 45, 1715-1718.
  • Babior BM (1984): The respiratory burst of phagocytes. J Clin Invest, 8, 599-601.
  • Batra TR, Singh K, Ho SK, Hıdıroglu M (1991): Concentration of plasma and milk vitamin E and plasma β- carotene of mastitic and healthy cows. J Vit Nutr Res, 62, 233-237.
  • Braun U, Forrer WF, Lutz H (1991): Selenium and vitamin E in blood sera of cows from farms with increased incidence of disease. Vet Rec, 128, 543-547.
  • Colitti M, Stradaidi G, Stefanon B (2000): Effect of α- tocopherol deprivation on the involution of mammary gland in sheep. J Dairy Sci, 83, 345-350.
  • Combs GF, Combs BS (1986): The Role of Selenium Nutrition. Academic Press. Inc London.
  • Craven N, Williams MR (1985): Defense of bovine mammary gland against infection and prospect for their enhancements. Vet Immunol Immunopat, 2, 71-78.
  • Çay M (1996): Ratlarda Selenyum ve Vitamin E’nin Alveolar ve Peritoneal Makrofajların Fagositik Aktivitesi Üzerindeki Etkisi. Fırat Üniversitesi Sağlık Bilimleri Ensti- tüsü Doktora Tezi, Elazığ.
  • Dowel LRM (1989): Vitamins in Animal Nutrition Comparative Aspects to Human Nutrition. Vitamin E. Academic Press, London
  • Dündar Y, Eryavuz A, Aslan R, Uçar M (2000): Malandialdehyde and glucose-6-phosphate dehydrogenase levels in healthy and subclinical mastitic cows, Yüzüncü Yıl Üniv Sağ Bil Derg, 6, 84-86.
  • Ergun H, Mert N (1984): Sütte mastitis nedeniyle meyda- na gelen biyokimyasal değişmeler. 1 Mastitis Semineri 24- Kasım Ankara Üniversitesi Veteriner Fakültesi Ankara.
  • Gornal AG, Bardawill CJ, David MM (1975): Determination of serum proteins by means of the biüret reaction. J Biol Chem, 177: 751.
  • Goth L (1991): A simple method for determination of serum catalase activity and revision of reference range. Clin Chim Acta, 196, 143-152.
  • Hogan JS, Smith KL, Weiss WP, Todhunter DA, Schockey WL (1990): Relationships among vitamin E, selenium and bovine blood neutrophils. J Dairy Sci, 73, 2372-2378.
  • Hogan JS, Weiss WP, Smith K, Soridillo LM, Williams SN (1996): α-tocopherol concentrations in milk and plasma during clinical Escherichia coli mastitis. J Dairy Sci, 79, 71-75.
  • Jain NC (1979): Common mammary pathogens and factors in infection and mastitis. J Dairy Sci, 62, 128-134.
  • Johnston LA, Chew BP (1984): Peripartum of plasma and milk vitamin A and β-carotene among dairy cows with or without mastitis. J Dairy Sci, 67, 1832-1840.
  • Kayden HJ, Chow CK, Bjarnson LK. (1973): Spectrophotometric method for determination of tocopherol in red blood cells. J Lip Res, 14, 533-540.
  • Lawrence RA, Burk RF (1976): Glutathione peroxidase activity in selenium-deficient rat liver. Bioch Bioph Res Commun, 71, 952-958.
  • Mates JM, Sanchez-Jimenes F (1999): Antioxidant enzymes and their implications in pathophsiologic processes. Front Biosci, 15, 339-345.
  • Matkovics B, Szabo I, Varga IS. (1988): Determination of enzyme activities in lipid peroxidation and glutathionep pathways (in Hungarian). Lab Diag, 15, 248-249.
  • Mayer SJ, Wterman AE, Keen PM, Craven N (1988): Oxygen concentration in milk of healthy and mastitic cows and implications of oxygen tension. J Dairy Sci, 55, 513- 519.
  • Morgante M, Beghelli D, Pausellim M, Dallara P, Capucella M, Ranucci S (1999): Effect of administration of vitamin E and selenium during the dary period on mammaray health and milk cell counts in dairy ewes. J Dairy Sci, 82, 623-631.
  • Ndiweni N, Field TR, Williams MR, Booth JM, Finc JM (1991): Studies on the incidence of clinical mastitis and blood levels of vitamin E and selenium in dairy herds in England. Vet Rec, 129, 86-88.
  • Nizamlıoğlu M, Dinç DA, Erganiş O, Özeren F, Uçan S. (1992): Subklinik mastitisli koyunlarda N- asetil B-D glikozaminidaz (NAG ase) enzimi ve bazı biyokimyasal de- ğerlerin araştırılması.Veterinarium, 3, 12-16.
  • Placer ZA, Cushmann LL, Johnson BC (1966): Estimation of products of lipid peroxidation in biochhemical systems. Anal Biochem, 16, 359-364.
  • Sandholm M, Mattila T (1986): Biochemical aspects of bovine mastitis. Isr J Vet Med, 42, 405-415.
  • Schalm OW, Carrol EJ, Jain NC (1971): Bovine Mastitis. First Ed-Lea-Febriger London.
  • Scholz RW, Hutchinson LJ (1979): Distribution of glutathione peroxidase activity and selenium in the blood of dairy cows. Am J Vet Res, 40, 245-249.
  • Sedlak J, Lindsay RHC (1968): Estimation of total prote- in bound and nonprotein sulfhydryl groups in tissue with Ellmann’s reagent. Anal Biochem, 25, 192-205.
  • Serfass RE, Ganther HE (1976): Effects of dietary selenium and tocopherol on glutathione peroxidase and superoxide dismutase activities in Rat. Phago-Life Sci, 19, 1139-1144.
  • Smith KL (1983): Mastitis control: A discussion. J Dairy Sci, 66, 1790-1794.
  • Smith KL, Conrad HR, Amiet BA, Todhunter DA (1985): Incidence of enviromental mastitis as influenced by dietary vitamin E and selenium. Kieler Milch Forsc Benic, 37, 482-486.
  • Stocchini A, Coni E, Baldini M, Beccalno E, Caroli S (1989): Selenium intake with diet in Italy. A plat study. J Trace Elem Electrol Health Dis, 3, 193-198.
  • Stratton SP, Liebler DC (1997): Determination of siglet oxygen-specific versus radical-mediated lipid peroxidation in photosensitized oxidation of lipid bilayers; effect of β- carotene and α-tocopherol. Biochemistry, 36, 12911- 12920.
  • Sümbüloğlu K, Sümbüloğlu V (1995): Biyoistatistik. Özdemir Basım Yayım ve Dağıtım LTD Şti 6. Baskı, An- kara.
  • Wuryastuti H, Stowe HD, Bull RW, Miller ER (1993): Effects of vitamin E and seleniım on immune responses of peripheral blood, colostrum and milk leukocytes of sows. J Anim Sci, 71, 2464-2472.
  • Yarım G, Salmanoğlu B (2002): Subklinik mastitisli süt ineklerinde meme içi levamizol uygulanmasında süt ve kanda glutatyon peroksidaz, süperoksit dismutaz, alkalen fosfotaz ve immunoglobulin G düzeyleri. Ankara Üniv Vet Fak Derg, 49, 89-94.
Toplam 45 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Veteriner Cerrahi
Bölüm Araştırma Makalesi
Yazarlar

Halil Şimşek

Mesut Aksakal

Yayımlanma Tarihi 1 Haziran 2005
Yayımlandığı Sayı Yıl 2005Cilt: 52 Sayı: 2

Kaynak Göster

APA Şimşek, H., & Aksakal, M. (2005). Subklinik mastitisli ineklerde kan ve sütte lipit peroksidasyon ve bazı antioksidanlar üzerine E vitamininin etkisi. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 52(2), 71-76. https://doi.org/10.1501/Vetfak_0000000039
AMA Şimşek H, Aksakal M. Subklinik mastitisli ineklerde kan ve sütte lipit peroksidasyon ve bazı antioksidanlar üzerine E vitamininin etkisi. Ankara Univ Vet Fak Derg. Haziran 2005;52(2):71-76. doi:10.1501/Vetfak_0000000039
Chicago Şimşek, Halil, ve Mesut Aksakal. “Subklinik Mastitisli Ineklerde Kan Ve sütte Lipit Peroksidasyon Ve Bazı Antioksidanlar üzerine E Vitamininin Etkisi”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 52, sy. 2 (Haziran 2005): 71-76. https://doi.org/10.1501/Vetfak_0000000039.
EndNote Şimşek H, Aksakal M (01 Haziran 2005) Subklinik mastitisli ineklerde kan ve sütte lipit peroksidasyon ve bazı antioksidanlar üzerine E vitamininin etkisi. Ankara Üniversitesi Veteriner Fakültesi Dergisi 52 2 71–76.
IEEE H. Şimşek ve M. Aksakal, “Subklinik mastitisli ineklerde kan ve sütte lipit peroksidasyon ve bazı antioksidanlar üzerine E vitamininin etkisi”, Ankara Univ Vet Fak Derg, c. 52, sy. 2, ss. 71–76, 2005, doi: 10.1501/Vetfak_0000000039.
ISNAD Şimşek, Halil - Aksakal, Mesut. “Subklinik Mastitisli Ineklerde Kan Ve sütte Lipit Peroksidasyon Ve Bazı Antioksidanlar üzerine E Vitamininin Etkisi”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 52/2 (Haziran 2005), 71-76. https://doi.org/10.1501/Vetfak_0000000039.
JAMA Şimşek H, Aksakal M. Subklinik mastitisli ineklerde kan ve sütte lipit peroksidasyon ve bazı antioksidanlar üzerine E vitamininin etkisi. Ankara Univ Vet Fak Derg. 2005;52:71–76.
MLA Şimşek, Halil ve Mesut Aksakal. “Subklinik Mastitisli Ineklerde Kan Ve sütte Lipit Peroksidasyon Ve Bazı Antioksidanlar üzerine E Vitamininin Etkisi”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, c. 52, sy. 2, 2005, ss. 71-76, doi:10.1501/Vetfak_0000000039.
Vancouver Şimşek H, Aksakal M. Subklinik mastitisli ineklerde kan ve sütte lipit peroksidasyon ve bazı antioksidanlar üzerine E vitamininin etkisi. Ankara Univ Vet Fak Derg. 2005;52(2):71-6.